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The map label for this gene is ywnE [H]

Identifier: 188585539

GI number: 188585539

Start: 965470

End: 966927

Strand: Reverse

Name: ywnE [H]

Synonym: Nther_0911

Alternate gene names: 188585539

Gene position: 966927-965470 (Counterclockwise)

Preceding gene: 188585547

Following gene: 188585535

Centisome position: 30.55

GC content: 34.98

Gene sequence:

>1458_bases
ATGTTGCTGGAATTGGTGGTTCTCATTGAATTAGTTTTAATTATCTCAGTTATATTTATTGAACGTAAACAACCCAGTGA
GGCATTCTCCTGGGTTTTAATAATTTTATTCCTACCTGGATTAGGTTATATCCTATACTTAATATTTGGAGAAACCTGGA
GTAAAAAAAGGGCTAAAAGATTGAAAGAAAGCGTTGTTAGAGATAATTACAAAAATGAATTATCAAGATACTATCAATAT
TTAGCTCAACACAATTATACGAAAAACGATTACTTTAACCCGTATGTTAAAGATTTAATAGACTTTCATAGAAATTATAG
TTACAGTTTATACACCTCAGACAATGATATCGAAGTTTTTACATGGGGGGAAGACAAATTTTCACGCCTTTTTCAAGATA
TAGACCAGGCTCAGGACAGTATTCATGTGATGTATTTTAAGATATATAGAGATAGCACCGGAAAGCAATTAGTTGACCAC
CTTACTCAAAAGGCCAAAGAAGGAATAAAAGTAAGGGTAATTTACGATGCCATCGGAAGTATGACACCAAAGTCTTTTTT
CCATCCTTTAATTGAAGCCGGTGGACAAGTTCAGTGTTATGAAGAATCCATTTTTAAAAACCTCTTAAGATTAAACTTCC
GTAATCACCGAAAAATGGCCATTATTGACGGTAAAATAGGTTATATGGGTGGAATGAATGTAGGCGACACCTACTTGAGC
CAGCATAAAATAAAAACACCCTGGCGTGATACCCACATGCGTATCACTGGTTCCAGCGTGTTAGTATTGCAGTTTTATTT
TTTTCAAGACTGGGCTCGATTAAGCTCTGACATAACCAAAGATTTACCCAGGGAAAAAATAGCTAGATATTTCCCTTCAA
ACTCCAGCTCTACCAGCACCACAAACACCAATGGATCTGGTATGCAACTGGTAATCAACAGCGCTGATACTGATAGTGAA
AGAATTAAGATGGGCATTATCAAAATGATTAATTCTGCCACGGAAAATATCCAAATTCAAAGCCCTTATTTCGTACCTGA
CAGGAGCATGATACAAGCTTTAAAAATTGCCATTTATTCTGGTGTAAAAGTTCAAATCATGATACCTGAAATTCCAAGCT
CCTTTTTTCTTAAACCAGTTTCCCTGTCATTTGCTGGTGAGTTGTTAGAAGCTGGTGCTGAAGTTTATTTATATCAGGGA
TATATACACGCAAAAACTGTCCAGATAGATGGAGAAGTCTGTACAATAGGTTCCACCAATATAGATATCAGGAGCTTTAG
GCTTAGTGATGAAATCAACGCTTTTATTTATGATAAAGATTTCTCCGAAAAACACAGGCAAATATTCTTACAAGATATTT
ACAACTCCTCTCCTCTTTATTGGGAAGAGTTTAAAAATAGAGGTGCCATAAGAAAAATAATTGAAGACATCTTAAGACTA
TTTTCACCTTTAATGTAA

Upstream 100 bases:

>100_bases
TCCTTAACTCCACTTTATATCAATGCTTTACTCTTTGAAAAAAATAAATAATTAAAATTTTATGACAATGAATTTCATAA
TAAAGAAAGGTTGATTATAT

Downstream 100 bases:

>100_bases
TATAGACTTGGCTCCAGAGTTATCGGGTTTTAATTTGAAAAAATTGCTCTCGATTCCGTGAGCACTAAATTTCTCTTTTC
CGAGCTCACGGACAATTTTT

Product: phospholipase D/Transphosphatidylase

Products: NA

Alternate protein names: CL synthase 2 [H]

Number of amino acids: Translated: 485; Mature: 485

Protein sequence:

>485_residues
MLLELVVLIELVLIISVIFIERKQPSEAFSWVLIILFLPGLGYILYLIFGETWSKKRAKRLKESVVRDNYKNELSRYYQY
LAQHNYTKNDYFNPYVKDLIDFHRNYSYSLYTSDNDIEVFTWGEDKFSRLFQDIDQAQDSIHVMYFKIYRDSTGKQLVDH
LTQKAKEGIKVRVIYDAIGSMTPKSFFHPLIEAGGQVQCYEESIFKNLLRLNFRNHRKMAIIDGKIGYMGGMNVGDTYLS
QHKIKTPWRDTHMRITGSSVLVLQFYFFQDWARLSSDITKDLPREKIARYFPSNSSSTSTTNTNGSGMQLVINSADTDSE
RIKMGIIKMINSATENIQIQSPYFVPDRSMIQALKIAIYSGVKVQIMIPEIPSSFFLKPVSLSFAGELLEAGAEVYLYQG
YIHAKTVQIDGEVCTIGSTNIDIRSFRLSDEINAFIYDKDFSEKHRQIFLQDIYNSSPLYWEEFKNRGAIRKIIEDILRL
FSPLM

Sequences:

>Translated_485_residues
MLLELVVLIELVLIISVIFIERKQPSEAFSWVLIILFLPGLGYILYLIFGETWSKKRAKRLKESVVRDNYKNELSRYYQY
LAQHNYTKNDYFNPYVKDLIDFHRNYSYSLYTSDNDIEVFTWGEDKFSRLFQDIDQAQDSIHVMYFKIYRDSTGKQLVDH
LTQKAKEGIKVRVIYDAIGSMTPKSFFHPLIEAGGQVQCYEESIFKNLLRLNFRNHRKMAIIDGKIGYMGGMNVGDTYLS
QHKIKTPWRDTHMRITGSSVLVLQFYFFQDWARLSSDITKDLPREKIARYFPSNSSSTSTTNTNGSGMQLVINSADTDSE
RIKMGIIKMINSATENIQIQSPYFVPDRSMIQALKIAIYSGVKVQIMIPEIPSSFFLKPVSLSFAGELLEAGAEVYLYQG
YIHAKTVQIDGEVCTIGSTNIDIRSFRLSDEINAFIYDKDFSEKHRQIFLQDIYNSSPLYWEEFKNRGAIRKIIEDILRL
FSPLM
>Mature_485_residues
MLLELVVLIELVLIISVIFIERKQPSEAFSWVLIILFLPGLGYILYLIFGETWSKKRAKRLKESVVRDNYKNELSRYYQY
LAQHNYTKNDYFNPYVKDLIDFHRNYSYSLYTSDNDIEVFTWGEDKFSRLFQDIDQAQDSIHVMYFKIYRDSTGKQLVDH
LTQKAKEGIKVRVIYDAIGSMTPKSFFHPLIEAGGQVQCYEESIFKNLLRLNFRNHRKMAIIDGKIGYMGGMNVGDTYLS
QHKIKTPWRDTHMRITGSSVLVLQFYFFQDWARLSSDITKDLPREKIARYFPSNSSSTSTTNTNGSGMQLVINSADTDSE
RIKMGIIKMINSATENIQIQSPYFVPDRSMIQALKIAIYSGVKVQIMIPEIPSSFFLKPVSLSFAGELLEAGAEVYLYQG
YIHAKTVQIDGEVCTIGSTNIDIRSFRLSDEINAFIYDKDFSEKHRQIFLQDIYNSSPLYWEEFKNRGAIRKIIEDILRL
FSPLM

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol [H]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=492, Percent_Identity=27.6422764227642, Blast_Score=163, Evalue=2e-41,
Organism=Escherichia coli, GI1787007, Length=343, Percent_Identity=26.530612244898, Blast_Score=114, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022924
- InterPro:   IPR015679
- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.-

Molecular weight: Translated: 56312; Mature: 56312

Theoretical pI: Translated: 8.45; Mature: 8.45

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLELVVLIELVLIISVIFIERKQPSEAFSWVLIILFLPGLGYILYLIFGETWSKKRAKR
CHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
LKESVVRDNYKNELSRYYQYLAQHNYTKNDYFNPYVKDLIDFHRNYSYSLYTSDNDIEVF
HHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCEEEE
TWGEDKFSRLFQDIDQAQDSIHVMYFKIYRDSTGKQLVDHLTQKAKEGIKVRVIYDAIGS
ECCCHHHHHHHHHHHHHCCCEEEEEEEEEECCCHHHHHHHHHHHHHCCCEEEEEEHHHCC
MTPKSFFHPLIEAGGQVQCYEESIFKNLLRLNFRNHRKMAIIDGKIGYMGGMNVGDTYLS
CCHHHHHHHHHHCCCCEEEHHHHHHHHHHHCCCCCCCEEEEEECCEECCCCCCCCHHHHH
QHKIKTPWRDTHMRITGSSVLVLQFYFFQDWARLSSDITKDLPREKIARYFPSNSSSTST
CCCCCCCCCCCEEEEECCEEEEHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCC
TNTNGSGMQLVINSADTDSERIKMGIIKMINSATENIQIQSPYFVPDRSMIQALKIAIYS
CCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEECCEECCCHHHHHHHHHHHHC
GVKVQIMIPEIPSSFFLKPVSLSFAGELLEAGAEVYLYQGYIHAKTVQIDGEVCTIGSTN
CCEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCEEEEEEEEEEEEEEEECCCEEEECCCC
IDIRSFRLSDEINAFIYDKDFSEKHRQIFLQDIYNSSPLYWEEFKNRGAIRKIIEDILRL
CEEEEEEECCCCCEEEECCCHHHHHHHHHHHHHHCCCCCCHHHHHCCCHHHHHHHHHHHH
FSPLM
HCCCC
>Mature Secondary Structure
MLLELVVLIELVLIISVIFIERKQPSEAFSWVLIILFLPGLGYILYLIFGETWSKKRAKR
CHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
LKESVVRDNYKNELSRYYQYLAQHNYTKNDYFNPYVKDLIDFHRNYSYSLYTSDNDIEVF
HHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCEEEE
TWGEDKFSRLFQDIDQAQDSIHVMYFKIYRDSTGKQLVDHLTQKAKEGIKVRVIYDAIGS
ECCCHHHHHHHHHHHHHCCCEEEEEEEEEECCCHHHHHHHHHHHHHCCCEEEEEEHHHCC
MTPKSFFHPLIEAGGQVQCYEESIFKNLLRLNFRNHRKMAIIDGKIGYMGGMNVGDTYLS
CCHHHHHHHHHHCCCCEEEHHHHHHHHHHHCCCCCCCEEEEEECCEECCCCCCCCHHHHH
QHKIKTPWRDTHMRITGSSVLVLQFYFFQDWARLSSDITKDLPREKIARYFPSNSSSTST
CCCCCCCCCCCEEEEECCEEEEHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCC
TNTNGSGMQLVINSADTDSERIKMGIIKMINSATENIQIQSPYFVPDRSMIQALKIAIYS
CCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEECCEECCCHHHHHHHHHHHHC
GVKVQIMIPEIPSSFFLKPVSLSFAGELLEAGAEVYLYQGYIHAKTVQIDGEVCTIGSTN
CCEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCEEEEEEEEEEEEEEEECCCEEEECCCC
IDIRSFRLSDEINAFIYDKDFSEKHRQIFLQDIYNSSPLYWEEFKNRGAIRKIIEDILRL
CEEEEEEECCCCCEEEECCCHHHHHHHHHHHHHHCCCCCCHHHHHCCCHHHHHHHHHHHH
FSPLM
HCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9150240; 9384377 [H]