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The map label for this gene is pulA [H]

Identifier: 188585496

GI number: 188585496

Start: 914676

End: 917006

Strand: Direct

Name: pulA [H]

Synonym: Nther_0868

Alternate gene names: 188585496

Gene position: 914676-917006 (Clockwise)

Preceding gene: 188585495

Following gene: 188585497

Centisome position: 28.89

GC content: 39.47

Gene sequence:

>2331_bases
ATGAACGAAATTCGTTATGGTCTACTTAAAGAAAGAACTAGGATAGAACTTGGTTTTGCTACCATCCATGGCCTGACAGT
GGAAGAATTAGAGGAACATTTGTCAATTACAGACCGCAATCAAGAGAGAATTTATTTTGATAAAGTTTTGATTGAAGATG
CTGAGGAGAGTGTTTCTGTCTACGGGGAGTTTGATGTCCATAAAGCACCTTACCAAGTAACGTTTCATGAAATGTCAGCT
ACGGTAATTGTAAGTTGGCAACTCAAAGATGAACTTTACGCTTATAATGGATCTTTAGGCCCGGAACTTCATCAAGATGG
ATCAGCTACATTGAAACTTTGGTCTCCTAGTGCAGATAATGTTTCCGTTATTTTATACGATCGCTATGACCAATATAAGA
TCGTAGCAAATAGTATTGATATGACTCGCGGTCAGCGCGGTGTATGGGAAGTGACATTAGATCAAAGGAATACGGGAATT
CATGACTTAACCGGTTATTATTATCACTATAAAATAAAACGTTGTGATGAAGTCGTGACTGCCCTAGACCCTTACGCCCC
TTCTATGGCAGCTTGGGATAGCAATCAAGAAAATAAACTTAGAATTGGCAAAGCTGCCATTGTGGATCCATCTTCAATAG
GGCCAGAGTTAGATTTTGCCCGTATCGAAGGGTTCAAAAAACGGGAAGATGCAATTATCTACGAAATCCACGTCCGTGAT
TTTACTTCTGATCCATCCCTTGACAGTGAATTGGAATCTCAGTTCGGGACTTTTTCTGCCTTTATTGAAAAACTTGATTA
TATTAAAAATTTGGGTGTTACACATATTCAATTACTCCCGGTGATGAACTATTACATTGTGGATGAACTTAATAATGATC
AGCGCTTCATGGAGTACTCCTCAGCCCACAATAACTATAATTGGGGTTATGATCCCCATAACTATTTTTCTTTAACAGGT
ATGTATTCAGAAAACCCCGAAGACCCAAAGCAGCGGATTAGAGAATTTAAAAATCTGGTAAATGAAATTCACCGCCGTGG
CATGGGTGTGATCTTGGATGCAGTTTATAATCACACAGCAAGGGTTCATATTTTCGAAGATTTAGAGCCTAATTATTATC
ATTTTATGGAAGCTGACGGGACACCTCGTACTAGTTTTGGAGGCGGTCGTCTGGGTACAACACATAAAATGGCTCGGCGC
ATTGTTGTGGATTCAATTTTGTACTGGGTTAAAGAGTTCAAAGTAGATGGATTTCGATTTGACATGATGGGTGACCATGA
CGCAGAAACTATCCAGATGGCTTACGATAAAGCTAAAGAGATTAACCCTGAAATTGTCATGATCGGTGAAGGTTGGTTGA
CTTTTGCCGGAGATCAATTAGATCCTGAAGTACCGGCGGCAGATCAACACTGGATGCAATTTACAAATAGTGTTGGTTGT
TTTTCCGATGAGTTTCGTAATGAATTAAAATCAGGTTTTGGAATGGAAGGTGAGCCTCGCTTTATCACTGGAGGCGGTCG
TCATATTGGGAAAATTTTTGATAATTTAAGGGCGAACCCTCATAATTTTAAGGCAACTAACCCTGGAGATGTAGTTCCCT
ATATTGCGGCTCATGACAATTTAACCCTGCACGATGTAATTGCATTTTCCATCCAAAAAGATCCCGAATACCATCAAGAA
GAAATTCAAAAACGTATTCGACTGGGAAATCTCATGACTCTGACTGCCCAGGGAACATCTTTTCTCCATGCAGGCCAAGA
ATTTGGGCGTACAAAACAATTTCGTCATCGAGATTATCAAGGATGGGTGGATGACCTACCAGCTAATTCCACATTTATGA
CCAATAAAGACGGTGAACCCTTTAATTTCCCTTATTTTATTCACAATTCTTATGATTCTTCTGATGCTGTAAATAAGTTT
GATTGGCAGAAAGCTACTAATATAGAAGAGTACCCGATGAATGTCATGACGCGAGAATTTACACGAGGTTTGATAAAACT
TCGCAGGTCAACAGATGCTTTCAGACATGGAACAATTTCAGAAATTGATGAACATGTGAAAATGATCCATGCTCCAGAGA
TTCATGATCACGACCTTTTAATTGGGTATAGAACCGAGTCTTCAGACGAAACTGAAGAATACCTGGTTTTTGTCAATGCA
GACGAACGTGAACGTGAATTGACTGTAACTGATTATGATTTAGCCCGGGGGGAAGTGATAGTGGATAGTAATGAAGCAGG
TACTGAAGAGGTCAAGGAGCCATCAGGATTTGAATTGACTTCTGATGGCATAAGAATTGATCCTTTATCTGCAGTAATTA
TTAGATTGTAA

Upstream 100 bases:

>100_bases
TTGTATGGTATTGAACTTTTTCTGGAAAACTAAAATTAAATACCCATCCACTAGATAATTAAGTTGTGTTATTTTATTTG
GAAAACTTTGGAGGTATAAC

Downstream 100 bases:

>100_bases
TCCGGTGAAGCATAGCTGATGTCTGGAGGGAGTATATGTTTGGACAATTATTTTTGGGAATTTATTTTATTATTAAAGGC
ATAGTAGAATTTAGGGTAAA

Product: pullulanase, extracellular

Products: NA

Alternate protein names: Alpha-dextrin endo-1,6-alpha-glucosidase; Pullulan 6-glucanohydrolase [H]

Number of amino acids: Translated: 776; Mature: 776

Protein sequence:

>776_residues
MNEIRYGLLKERTRIELGFATIHGLTVEELEEHLSITDRNQERIYFDKVLIEDAEESVSVYGEFDVHKAPYQVTFHEMSA
TVIVSWQLKDELYAYNGSLGPELHQDGSATLKLWSPSADNVSVILYDRYDQYKIVANSIDMTRGQRGVWEVTLDQRNTGI
HDLTGYYYHYKIKRCDEVVTALDPYAPSMAAWDSNQENKLRIGKAAIVDPSSIGPELDFARIEGFKKREDAIIYEIHVRD
FTSDPSLDSELESQFGTFSAFIEKLDYIKNLGVTHIQLLPVMNYYIVDELNNDQRFMEYSSAHNNYNWGYDPHNYFSLTG
MYSENPEDPKQRIREFKNLVNEIHRRGMGVILDAVYNHTARVHIFEDLEPNYYHFMEADGTPRTSFGGGRLGTTHKMARR
IVVDSILYWVKEFKVDGFRFDMMGDHDAETIQMAYDKAKEINPEIVMIGEGWLTFAGDQLDPEVPAADQHWMQFTNSVGC
FSDEFRNELKSGFGMEGEPRFITGGGRHIGKIFDNLRANPHNFKATNPGDVVPYIAAHDNLTLHDVIAFSIQKDPEYHQE
EIQKRIRLGNLMTLTAQGTSFLHAGQEFGRTKQFRHRDYQGWVDDLPANSTFMTNKDGEPFNFPYFIHNSYDSSDAVNKF
DWQKATNIEEYPMNVMTREFTRGLIKLRRSTDAFRHGTISEIDEHVKMIHAPEIHDHDLLIGYRTESSDETEEYLVFVNA
DERERELTVTDYDLARGEVIVDSNEAGTEEVKEPSGFELTSDGIRIDPLSAVIIRL

Sequences:

>Translated_776_residues
MNEIRYGLLKERTRIELGFATIHGLTVEELEEHLSITDRNQERIYFDKVLIEDAEESVSVYGEFDVHKAPYQVTFHEMSA
TVIVSWQLKDELYAYNGSLGPELHQDGSATLKLWSPSADNVSVILYDRYDQYKIVANSIDMTRGQRGVWEVTLDQRNTGI
HDLTGYYYHYKIKRCDEVVTALDPYAPSMAAWDSNQENKLRIGKAAIVDPSSIGPELDFARIEGFKKREDAIIYEIHVRD
FTSDPSLDSELESQFGTFSAFIEKLDYIKNLGVTHIQLLPVMNYYIVDELNNDQRFMEYSSAHNNYNWGYDPHNYFSLTG
MYSENPEDPKQRIREFKNLVNEIHRRGMGVILDAVYNHTARVHIFEDLEPNYYHFMEADGTPRTSFGGGRLGTTHKMARR
IVVDSILYWVKEFKVDGFRFDMMGDHDAETIQMAYDKAKEINPEIVMIGEGWLTFAGDQLDPEVPAADQHWMQFTNSVGC
FSDEFRNELKSGFGMEGEPRFITGGGRHIGKIFDNLRANPHNFKATNPGDVVPYIAAHDNLTLHDVIAFSIQKDPEYHQE
EIQKRIRLGNLMTLTAQGTSFLHAGQEFGRTKQFRHRDYQGWVDDLPANSTFMTNKDGEPFNFPYFIHNSYDSSDAVNKF
DWQKATNIEEYPMNVMTREFTRGLIKLRRSTDAFRHGTISEIDEHVKMIHAPEIHDHDLLIGYRTESSDETEEYLVFVNA
DERERELTVTDYDLARGEVIVDSNEAGTEEVKEPSGFELTSDGIRIDPLSAVIIRL
>Mature_776_residues
MNEIRYGLLKERTRIELGFATIHGLTVEELEEHLSITDRNQERIYFDKVLIEDAEESVSVYGEFDVHKAPYQVTFHEMSA
TVIVSWQLKDELYAYNGSLGPELHQDGSATLKLWSPSADNVSVILYDRYDQYKIVANSIDMTRGQRGVWEVTLDQRNTGI
HDLTGYYYHYKIKRCDEVVTALDPYAPSMAAWDSNQENKLRIGKAAIVDPSSIGPELDFARIEGFKKREDAIIYEIHVRD
FTSDPSLDSELESQFGTFSAFIEKLDYIKNLGVTHIQLLPVMNYYIVDELNNDQRFMEYSSAHNNYNWGYDPHNYFSLTG
MYSENPEDPKQRIREFKNLVNEIHRRGMGVILDAVYNHTARVHIFEDLEPNYYHFMEADGTPRTSFGGGRLGTTHKMARR
IVVDSILYWVKEFKVDGFRFDMMGDHDAETIQMAYDKAKEINPEIVMIGEGWLTFAGDQLDPEVPAADQHWMQFTNSVGC
FSDEFRNELKSGFGMEGEPRFITGGGRHIGKIFDNLRANPHNFKATNPGDVVPYIAAHDNLTLHDVIAFSIQKDPEYHQE
EIQKRIRLGNLMTLTAQGTSFLHAGQEFGRTKQFRHRDYQGWVDDLPANSTFMTNKDGEPFNFPYFIHNSYDSSDAVNKF
DWQKATNIEEYPMNVMTREFTRGLIKLRRSTDAFRHGTISEIDEHVKMIHAPEIHDHDLLIGYRTESSDETEEYLVFVNA
DERERELTVTDYDLARGEVIVDSNEAGTEEVKEPSGFELTSDGIRIDPLSAVIIRL

Specific function: Hydrolyzes The Alpha-1,6-Glucosidic Linkages In Glycogen Which Has First Been Partially Depolymerized By Phosphorylase. Shows Only Very Little Activity With Native Glycogen. [C]

COG id: COG1523

COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI2367229, Length=413, Percent_Identity=26.1501210653753, Blast_Score=96, Evalue=7e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783
- InterPro:   IPR014756
- InterPro:   IPR005323
- InterPro:   IPR011840 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48; PF03714 PUD [H]

EC number: =3.2.1.41 [H]

Molecular weight: Translated: 89390; Mature: 89390

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS00430 TONB_DEPENDENT_REC_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEIRYGLLKERTRIELGFATIHGLTVEELEEHLSITDRNQERIYFDKVLIEDAEESVSV
CCCCHHHHHCCCCEEEEEEEEECCCCHHHHHHHCCCCCCCCCEEEEEHHHHHCCCCCEEE
YGEFDVHKAPYQVTFHEMSATVIVSWQLKDELYAYNGSLGPELHQDGSATLKLWSPSADN
EEEECCCCCCEEEEEEECCEEEEEEEEECCEEEEECCCCCCCCCCCCCCEEEEECCCCCC
VSVILYDRYDQYKIVANSIDMTRGQRGVWEVTLDQRNTGIHDLTGYYYHYKIKRCDEVVT
EEEEEEECCCCEEEEECCCCCCCCCCCEEEEEECCCCCCCCCCCCEEEEEEEHHHHHHHH
ALDPYAPSMAAWDSNQENKLRIGKAAIVDPSSIGPELDFARIEGFKKREDAIIYEIHVRD
HCCCCCCCHHCCCCCCCCEEEECEEEEECCHHCCCCCCHHHHCCCCCCCCEEEEEEEEEE
FTSDPSLDSELESQFGTFSAFIEKLDYIKNLGVTHIQLLPVMNYYIVDELNNDQRFMEYS
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHCEEEEECCCCCHHHHHHH
SAHNNYNWGYDPHNYFSLTGMYSENPEDPKQRIREFKNLVNEIHRRGMGVILDAVYNHTA
HCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEHHHHCCCE
RVHIFEDLEPNYYHFMEADGTPRTSFGGGRLGTTHKMARRIVVDSILYWVKEFKVDGFRF
EEEEEECCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEE
DMMGDHDAETIQMAYDKAKEINPEIVMIGEGWLTFAGDQLDPEVPAADQHWMQFTNSVGC
ECCCCCCHHHHHHHHHHHHCCCCCEEEECCCEEEECCCCCCCCCCCCHHHHHHHHCCCCC
FSDEFRNELKSGFGMEGEPRFITGGGRHIGKIFDNLRANPHNFKATNPGDVVPYIAAHDN
CHHHHHHHHHHCCCCCCCCCEEECCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCC
LTLHDVIAFSIQKDPEYHQEEIQKRIRLGNLMTLTAQGTSFLHAGQEFGRTKQFRHRDYQ
CEEEHEEEEEECCCCCHHHHHHHHHHHCCCEEEEEECCCHHHHCCHHHCCCHHHHCCCCC
GWVDDLPANSTFMTNKDGEPFNFPYFIHNSYDSSDAVNKFDWQKATNIEEYPMNVMTREF
CCHHCCCCCCEEEECCCCCCCCCCEEEECCCCCCCCCCCCCCHHCCCCHHCCHHHHHHHH
TRGLIKLRRSTDAFRHGTISEIDEHVKMIHAPEIHDHDLLIGYRTESSDETEEYLVFVNA
HHHHHHHHHHCHHHHCCCHHHHHHHHHHEECCCCCCCEEEEEEECCCCCCCEEEEEEECC
DERERELTVTDYDLARGEVIVDSNEAGTEEVKEPSGFELTSDGIRIDPLSAVIIRL
CCCCCEEEEEECCCCCCEEEEECCCCCHHHHCCCCCCEEECCCEEECCHHEEEEEC
>Mature Secondary Structure
MNEIRYGLLKERTRIELGFATIHGLTVEELEEHLSITDRNQERIYFDKVLIEDAEESVSV
CCCCHHHHHCCCCEEEEEEEEECCCCHHHHHHHCCCCCCCCCEEEEEHHHHHCCCCCEEE
YGEFDVHKAPYQVTFHEMSATVIVSWQLKDELYAYNGSLGPELHQDGSATLKLWSPSADN
EEEECCCCCCEEEEEEECCEEEEEEEEECCEEEEECCCCCCCCCCCCCCEEEEECCCCCC
VSVILYDRYDQYKIVANSIDMTRGQRGVWEVTLDQRNTGIHDLTGYYYHYKIKRCDEVVT
EEEEEEECCCCEEEEECCCCCCCCCCCEEEEEECCCCCCCCCCCCEEEEEEEHHHHHHHH
ALDPYAPSMAAWDSNQENKLRIGKAAIVDPSSIGPELDFARIEGFKKREDAIIYEIHVRD
HCCCCCCCHHCCCCCCCCEEEECEEEEECCHHCCCCCCHHHHCCCCCCCCEEEEEEEEEE
FTSDPSLDSELESQFGTFSAFIEKLDYIKNLGVTHIQLLPVMNYYIVDELNNDQRFMEYS
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHCEEEEECCCCCHHHHHHH
SAHNNYNWGYDPHNYFSLTGMYSENPEDPKQRIREFKNLVNEIHRRGMGVILDAVYNHTA
HCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEHHHHCCCE
RVHIFEDLEPNYYHFMEADGTPRTSFGGGRLGTTHKMARRIVVDSILYWVKEFKVDGFRF
EEEEEECCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEE
DMMGDHDAETIQMAYDKAKEINPEIVMIGEGWLTFAGDQLDPEVPAADQHWMQFTNSVGC
ECCCCCCHHHHHHHHHHHHCCCCCEEEECCCEEEECCCCCCCCCCCCHHHHHHHHCCCCC
FSDEFRNELKSGFGMEGEPRFITGGGRHIGKIFDNLRANPHNFKATNPGDVVPYIAAHDN
CHHHHHHHHHHCCCCCCCCCEEECCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCC
LTLHDVIAFSIQKDPEYHQEEIQKRIRLGNLMTLTAQGTSFLHAGQEFGRTKQFRHRDYQ
CEEEHEEEEEECCCCCHHHHHHHHHHHCCCEEEEEECCCHHHHCCHHHCCCHHHHCCCCC
GWVDDLPANSTFMTNKDGEPFNFPYFIHNSYDSSDAVNKFDWQKATNIEEYPMNVMTREF
CCHHCCCCCCEEEECCCCCCCCCCEEEECCCCCCCCCCCCCCHHCCCCHHCCHHHHHHHH
TRGLIKLRRSTDAFRHGTISEIDEHVKMIHAPEIHDHDLLIGYRTESSDETEEYLVFVNA
HHHHHHHHHHCHHHHCCCHHHHHHHHHHEECCCCCCCEEEEEEECCCCCCCEEEEEEECC
DERERELTVTDYDLARGEVIVDSNEAGTEEVKEPSGFELTSDGIRIDPLSAVIIRL
CCCCCEEEEEECCCCCCEEEEECCCCCHHHHCCCCCCEEECCCEEECCHHEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9453151; 10360571 [H]