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The map label for this gene is mcrB [C]

Identifier: 188585418

GI number: 188585418

Start: 818982

End: 820988

Strand: Direct

Name: mcrB [C]

Synonym: Nther_0788

Alternate gene names: 188585418

Gene position: 818982-820988 (Clockwise)

Preceding gene: 188585417

Following gene: 188585419

Centisome position: 25.87

GC content: 29.4

Gene sequence:

>2007_bases
ATGTCAATTGTTAATAGATTTGATGAAAAGTTTAAACAACGGCAAACTTCATATAAGATGGTTACTGTTTTAGCATTATT
GGAAAATGTAACTACAGATGGGAGTGCAGACTTCGATAATGTTGTTAACAGTTTTTTGAACTTTTATTTAGATAGACAAG
AGAAAGGTAAGAATCCAGAAATTGAAAGAGCTAAAATGTCTATACCTGAACAACTTTCTTCATCTCAAATTAAGAATACT
ATATTAAAAGGTCCTGTTACTCACTTAGAAGAGCTCATAGAATTTGATCCAGAAAACAATCAAATAAAATTTTCAGATAA
TACATATAAAGATCTTAATCAAAATGATAACAGAATGGAGTTAAAAAAAGTAGCTTATAAGCATCTTTATCAATATTACA
AAAATATAGATAATAATTGGATTAAGTTAGAAGATTTGTCTGACTTAGATCGTGGGTACCCAGTAAATGCAAATGACATT
TCTTCATTATCAGAACAAAACTCAATTAAAGGAATTCATCCAGTTGATTCACAAGAATTCCAAGGTACTATAATTTTATG
TACAATTAGTGGACAAGAATATCCTAATGAGTGGCTGGATGCAAATAAACAGTTTTTAAAATATTATTTAGAGGGTCGAA
GGGATAAAAATACTGGAATAAAAAAATATAACCAAAATTCCAAAAATAATCAATCTGTCATAAAGTCTAAAGAAGAAGGA
TATCCTATTTTGGCATTTGTCAGATATTCTAAAAACTCATACTTTTACTATGAAGGTCAATTTGTATTTGAAGAAATCAG
AAGAGATGAACAAGAACATCCTTATTTTGTTTTAAAAAGACAAGATCAAGCGAGTAATGAAATAGAATTTAATCAATCTG
TAAATACATGGGAGTCAATGAATTTTATTACAGAAGACAACAGCGAATATAATGGCTATAGCGAATATAATAGCTTTGAT
GTTGAAAGTCTTAATGAAGTCTATCCCCATATTATTTCTTTCATTAGAAATAGAGGTTTTGTTTTCTCTGATGACTTAAT
AAAAAATTTTTTATTAGCTCTAAAAAGTAAACCTTTTGTTATATTAGCAGGTTTATCAGGAACAGGAAAAAGTAAATTAG
TAGAATTAGTGGCCAATTCACTAGGAGCAACTAGAGAAAATGGACAGTTTACTCTTATTCCAGTACGTCCAGATTGGAAT
GATAGTACTGATTTACTAGGTTATAAAAACTTGCAAGGTGATTTTATTGAAGGTGAACTCACAAAAGTGATTAGAAAAGC
GAAAGCAAATCAAGATCACCCTTATATTGTCTGTTTAGATGAAATGAACTTAGCAAGAGTGGAATACTATTTTAGCGATA
TCTTAAGTGTTATGGAAACACGGAAAAAACAAGGTGAAAGAATAATAACTGCACCCATGCATGTTGAAGGATTAGATGAT
TCCCTAACTTTCCCGGACAATTTATACATTGTTGGTACTGTAAACATGGATGAAACTACTCATTCTTTTAGTCCTAAGGT
TCTCGATCGAGCTAATACAATTGAATTTAATGAAGTAAATTTAGACTATCTCCCTGATCATGAAGGAGAAGAAATATTAC
CCAAAAAAGCTCATAACAATATATTTAAAACTGAATATATAAAGTTAAAAGATTGCTATTATGGAAATGAAGATTTTATC
AAACAGCAAGTAGAGTTTTTAAAACAATTAAACTCAATTCTTAATAAACCAGGTTTACAACTTCAAATAGGATATCGTAT
ACGAGATGAATTTTGCTTTTATTTGTTAAATAATAATAAATTTAAATTACTTCCTGAAAACACTGCTAAAGACTTTCAAA
TAATGCAAAAAATATTGCCAAGAATTCAGGGGAGCTCATTAAAAATTGAATCCGTATTAAAAGAACTGATAGAACTGTGT
TCAGACAGTTTGCCACACAGTAAAGAAAAAGCAGAATTTATGTTAGAAAGGTTTGAAGAAGATGGCTTCACTTCATTCTG
GCTCTAA

Upstream 100 bases:

>100_bases
GCACTAATGGGGATGTCTTATTTAGAAATTGATAAAACGCAAGTATCTCAAATTCAAAGGGTTGCATAATAAAATAATTT
TAATATTTAGGAGTGTGATT

Downstream 100 bases:

>100_bases
ACTTAAAGAATTACTAGTTATAGAAACTGATAGATTTACATTGTCAATCAAAGGCTTGCCTGTAAATAAAAATAACGATG
GAAAGCTACATAATATCAAG

Product: ATPase associated with various cellular activities AAA_5

Products: NA

Alternate protein names: GTPase Subunit Of Restriction Endonuclease-Like Protein; 5-Methylcytosine-Specific Restriction ; 5-Methylcytosine-Specific Restriction McrB Related Protein; GTPase Subunit Of Restriction Endonuclease-Like; Site-Specific DNA-Methyltransferase Cytosine-Specific; 5-Methylcytosine-Specific Restriction-Like Protein; AAA Family ATPase; AAA ATPase; ATPase AAA Family

Number of amino acids: Translated: 668; Mature: 667

Protein sequence:

>668_residues
MSIVNRFDEKFKQRQTSYKMVTVLALLENVTTDGSADFDNVVNSFLNFYLDRQEKGKNPEIERAKMSIPEQLSSSQIKNT
ILKGPVTHLEELIEFDPENNQIKFSDNTYKDLNQNDNRMELKKVAYKHLYQYYKNIDNNWIKLEDLSDLDRGYPVNANDI
SSLSEQNSIKGIHPVDSQEFQGTIILCTISGQEYPNEWLDANKQFLKYYLEGRRDKNTGIKKYNQNSKNNQSVIKSKEEG
YPILAFVRYSKNSYFYYEGQFVFEEIRRDEQEHPYFVLKRQDQASNEIEFNQSVNTWESMNFITEDNSEYNGYSEYNSFD
VESLNEVYPHIISFIRNRGFVFSDDLIKNFLLALKSKPFVILAGLSGTGKSKLVELVANSLGATRENGQFTLIPVRPDWN
DSTDLLGYKNLQGDFIEGELTKVIRKAKANQDHPYIVCLDEMNLARVEYYFSDILSVMETRKKQGERIITAPMHVEGLDD
SLTFPDNLYIVGTVNMDETTHSFSPKVLDRANTIEFNEVNLDYLPDHEGEEILPKKAHNNIFKTEYIKLKDCYYGNEDFI
KQQVEFLKQLNSILNKPGLQLQIGYRIRDEFCFYLLNNNKFKLLPENTAKDFQIMQKILPRIQGSSLKIESVLKELIELC
SDSLPHSKEKAEFMLERFEEDGFTSFWL

Sequences:

>Translated_668_residues
MSIVNRFDEKFKQRQTSYKMVTVLALLENVTTDGSADFDNVVNSFLNFYLDRQEKGKNPEIERAKMSIPEQLSSSQIKNT
ILKGPVTHLEELIEFDPENNQIKFSDNTYKDLNQNDNRMELKKVAYKHLYQYYKNIDNNWIKLEDLSDLDRGYPVNANDI
SSLSEQNSIKGIHPVDSQEFQGTIILCTISGQEYPNEWLDANKQFLKYYLEGRRDKNTGIKKYNQNSKNNQSVIKSKEEG
YPILAFVRYSKNSYFYYEGQFVFEEIRRDEQEHPYFVLKRQDQASNEIEFNQSVNTWESMNFITEDNSEYNGYSEYNSFD
VESLNEVYPHIISFIRNRGFVFSDDLIKNFLLALKSKPFVILAGLSGTGKSKLVELVANSLGATRENGQFTLIPVRPDWN
DSTDLLGYKNLQGDFIEGELTKVIRKAKANQDHPYIVCLDEMNLARVEYYFSDILSVMETRKKQGERIITAPMHVEGLDD
SLTFPDNLYIVGTVNMDETTHSFSPKVLDRANTIEFNEVNLDYLPDHEGEEILPKKAHNNIFKTEYIKLKDCYYGNEDFI
KQQVEFLKQLNSILNKPGLQLQIGYRIRDEFCFYLLNNNKFKLLPENTAKDFQIMQKILPRIQGSSLKIESVLKELIELC
SDSLPHSKEKAEFMLERFEEDGFTSFWL
>Mature_667_residues
SIVNRFDEKFKQRQTSYKMVTVLALLENVTTDGSADFDNVVNSFLNFYLDRQEKGKNPEIERAKMSIPEQLSSSQIKNTI
LKGPVTHLEELIEFDPENNQIKFSDNTYKDLNQNDNRMELKKVAYKHLYQYYKNIDNNWIKLEDLSDLDRGYPVNANDIS
SLSEQNSIKGIHPVDSQEFQGTIILCTISGQEYPNEWLDANKQFLKYYLEGRRDKNTGIKKYNQNSKNNQSVIKSKEEGY
PILAFVRYSKNSYFYYEGQFVFEEIRRDEQEHPYFVLKRQDQASNEIEFNQSVNTWESMNFITEDNSEYNGYSEYNSFDV
ESLNEVYPHIISFIRNRGFVFSDDLIKNFLLALKSKPFVILAGLSGTGKSKLVELVANSLGATRENGQFTLIPVRPDWND
STDLLGYKNLQGDFIEGELTKVIRKAKANQDHPYIVCLDEMNLARVEYYFSDILSVMETRKKQGERIITAPMHVEGLDDS
LTFPDNLYIVGTVNMDETTHSFSPKVLDRANTIEFNEVNLDYLPDHEGEEILPKKAHNNIFKTEYIKLKDCYYGNEDFIK
QQVEFLKQLNSILNKPGLQLQIGYRIRDEFCFYLLNNNKFKLLPENTAKDFQIMQKILPRIQGSSLKIESVLKELIELCS
DSLPHSKEKAEFMLERFEEDGFTSFWL

Specific function: Recognizes N4- And C5-Methylcytosine (And 5-Hydroxy- Methylcytosines) Produced By A Broad Range Of DNA Methylases And Appears To Act Against 5-Methylcytosine Preceded By A Purine Residue. Binds To DNA Containing Methylated Cytosines; Also Binds To GTP. [

COG id: COG1401

COG function: function code V; GTPase subunit of restriction endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 3.1.21.- [C]

Molecular weight: Translated: 77890; Mature: 77758

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIVNRFDEKFKQRQTSYKMVTVLALLENVTTDGSADFDNVVNSFLNFYLDRQEKGKNPE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCHHHCCCCCC
IERAKMSIPEQLSSSQIKNTILKGPVTHLEELIEFDPENNQIKFSDNTYKDLNQNDNRME
HHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCEEEECCCCHHCCCCCCCHHH
LKKVAYKHLYQYYKNIDNNWIKLEDLSDLDRGYPVNANDISSLSEQNSIKGIHPVDSQEF
HHHHHHHHHHHHHHCCCCCEEEECCCHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCC
QGTIILCTISGQEYPNEWLDANKQFLKYYLEGRRDKNTGIKKYNQNSKNNQSVIKSKEEG
CCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCCCCCCHHHHHHHCCC
YPILAFVRYSKNSYFYYEGQFVFEEIRRDEQEHPYFVLKRQDQASNEIEFNQSVNTWESM
CCEEEEEEECCCEEEEECCHHHHHHHHCCCCCCCEEEEEECCCCCCCEEECCCCCCHHHC
NFITEDNSEYNGYSEYNSFDVESLNEVYPHIISFIRNRGFVFSDDLIKNFLLALKSKPFV
CEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHCCCCEE
ILAGLSGTGKSKLVELVANSLGATRENGQFTLIPVRPDWNDSTDLLGYKNLQGDFIEGEL
EEECCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCHHHH
TKVIRKAKANQDHPYIVCLDEMNLARVEYYFSDILSVMETRKKQGERIITAPMHVEGLDD
HHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCEEECCCCC
SLTFPDNLYIVGTVNMDETTHSFSPKVLDRANTIEFNEVNLDYLPDHEGEEILPKKAHNN
CCCCCCCEEEEEEECCCCCCCCCCCHHHCCCCCEEEEEECCCCCCCCCCCCCCCHHHCCC
IFKTEYIKLKDCYYGNEDFIKQQVEFLKQLNSILNKPGLQLQIGYRIRDEFCFYLLNNNK
CEEEHEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCEEEEEECCCE
FKLLPENTAKDFQIMQKILPRIQGSSLKIESVLKELIELCSDSLPHSKEKAEFMLERFEE
EEECCCCCHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHC
DGFTSFWL
CCCEECCC
>Mature Secondary Structure 
SIVNRFDEKFKQRQTSYKMVTVLALLENVTTDGSADFDNVVNSFLNFYLDRQEKGKNPE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCHHHCCCCCC
IERAKMSIPEQLSSSQIKNTILKGPVTHLEELIEFDPENNQIKFSDNTYKDLNQNDNRME
HHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCEEEECCCCHHCCCCCCCHHH
LKKVAYKHLYQYYKNIDNNWIKLEDLSDLDRGYPVNANDISSLSEQNSIKGIHPVDSQEF
HHHHHHHHHHHHHHCCCCCEEEECCCHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCC
QGTIILCTISGQEYPNEWLDANKQFLKYYLEGRRDKNTGIKKYNQNSKNNQSVIKSKEEG
CCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCCCCCCHHHHHHHCCC
YPILAFVRYSKNSYFYYEGQFVFEEIRRDEQEHPYFVLKRQDQASNEIEFNQSVNTWESM
CCEEEEEEECCCEEEEECCHHHHHHHHCCCCCCCEEEEEECCCCCCCEEECCCCCCHHHC
NFITEDNSEYNGYSEYNSFDVESLNEVYPHIISFIRNRGFVFSDDLIKNFLLALKSKPFV
CEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHCCCCEE
ILAGLSGTGKSKLVELVANSLGATRENGQFTLIPVRPDWNDSTDLLGYKNLQGDFIEGEL
EEECCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCHHHH
TKVIRKAKANQDHPYIVCLDEMNLARVEYYFSDILSVMETRKKQGERIITAPMHVEGLDD
HHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCEEECCCCC
SLTFPDNLYIVGTVNMDETTHSFSPKVLDRANTIEFNEVNLDYLPDHEGEEILPKKAHNN
CCCCCCCEEEEEEECCCCCCCCCCCHHHCCCCCEEEEEECCCCCCCCCCCCCCCHHHCCC
IFKTEYIKLKDCYYGNEDFIKQQVEFLKQLNSILNKPGLQLQIGYRIRDEFCFYLLNNNK
CEEEHEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCEEEEEECCCE
FKLLPENTAKDFQIMQKILPRIQGSSLKIESVLKELIELCSDSLPHSKEKAEFMLERFEE
EEECCCCCHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHC
DGFTSFWL
CCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds; Endodeoxyribonucleases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA