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The map label for this gene is fadH [C]

Identifier: 188585401

GI number: 188585401

Start: 799177

End: 800169

Strand: Reverse

Name: fadH [C]

Synonym: Nther_0771

Alternate gene names: 188585401

Gene position: 800169-799177 (Counterclockwise)

Preceding gene: 188585412

Following gene: 188585394

Centisome position: 25.28

GC content: 41.09

Gene sequence:

>993_bases
ATGTCACATTTACTTAAATCATTGGAAATCGGTCCATTAAGTTTAAAGAATCGTCTGGTTATGCCACCTATGGCTACAGC
AAAGGCTGAACCTGATGGATCTGTTAATCAGTCTATCTTTGACTACTATTCCGAAAAATCTGATGGGGGATATATCTCAT
TAATCATAGTCGAGCACAGTTTTATCTCCCATGAAGGTCAAGCTAGCGAATATCAAGTTTCTATCGCTGATGATCAGAAA
ATAGATGGTTTGGAAAAGTTAGCAGAGATTATTCAAAACAATGGTTCTAAGGCAGTAATGCAAATAAATCACGCCGGGAG
TGCAGCCCCCGAAGATATCATAGGGACAAAACCCGTAGGACCGTCGGCAGTTCCAAACCCACGCAAAGGAAATACCCCCC
ATGAGCTCACTGAAAAAGAGATTCAAGATATTGTGGAAGCTTTTAGCAATGCTGCCAGAAGAACTAAAGAAGCTGGATTT
GACGGTGTAGAAATTCATTCTGCCCATGGTTATCTGCTTAATCAGTTTTATTCACCCCTTACTAACAAGAGAACAGATAA
ATATGGCGGAGATGTACATAAGCGAATTAGAATTCACCTTGAGGTAATTGAAGCAGTTAAAAAAGAAGTTGGAGAAGATT
TCCCTATTCTTTTACGACTCGGAGCTTCAGATTATCAAGAAGGTGGCACTACTATTGAAGACAGTAAAATAGCAGCCAGG
GAATTTGAGAAAGCGGGTGTAGATATTCTTGATATTTCGGGAGGCCATTCGGGATATATGGTGAATGGTCTTGACGGACA
GGGTTTCTTCGCTCCACTGACAGAAGCTATTAAAGAAGTAGTGTCAATTCCGGTTATCCTAACTGGTGGAATTACAGAAC
CCGAGGCAGCCGAAAAACTACTTATTGAAGGGAAAGCTGATTTAATTGGGGTAGGTAGAGCTATACTCGAGGATTCCACT
TGGGCTAAACGGGCCATTGAATCCCTACAATAA

Upstream 100 bases:

>100_bases
CTGTATAATTTGTAAGGGAAAACCCCGACTTAGCTTAGTTAGCAGGATTAAAGTAGTTATAATAGAATATAAAACTTATA
GTACTAAAGGAGGAATCTTT

Downstream 100 bases:

>100_bases
AAACAAAACCAGCCCTTAGGAGCACTTTGATATGCTCCTCAGGGGCTGGATAGTCAACACTTAATTGGACAATTATTTTA
AGGTACTAGACTTATATTCA

Product: NADH:flavin oxidoreductase/NADH oxidase

Products: trans-transtetradehydroacyl-CoA; NADPH [C]

Alternate protein names: NA

Number of amino acids: Translated: 330; Mature: 329

Protein sequence:

>330_residues
MSHLLKSLEIGPLSLKNRLVMPPMATAKAEPDGSVNQSIFDYYSEKSDGGYISLIIVEHSFISHEGQASEYQVSIADDQK
IDGLEKLAEIIQNNGSKAVMQINHAGSAAPEDIIGTKPVGPSAVPNPRKGNTPHELTEKEIQDIVEAFSNAARRTKEAGF
DGVEIHSAHGYLLNQFYSPLTNKRTDKYGGDVHKRIRIHLEVIEAVKKEVGEDFPILLRLGASDYQEGGTTIEDSKIAAR
EFEKAGVDILDISGGHSGYMVNGLDGQGFFAPLTEAIKEVVSIPVILTGGITEPEAAEKLLIEGKADLIGVGRAILEDST
WAKRAIESLQ

Sequences:

>Translated_330_residues
MSHLLKSLEIGPLSLKNRLVMPPMATAKAEPDGSVNQSIFDYYSEKSDGGYISLIIVEHSFISHEGQASEYQVSIADDQK
IDGLEKLAEIIQNNGSKAVMQINHAGSAAPEDIIGTKPVGPSAVPNPRKGNTPHELTEKEIQDIVEAFSNAARRTKEAGF
DGVEIHSAHGYLLNQFYSPLTNKRTDKYGGDVHKRIRIHLEVIEAVKKEVGEDFPILLRLGASDYQEGGTTIEDSKIAAR
EFEKAGVDILDISGGHSGYMVNGLDGQGFFAPLTEAIKEVVSIPVILTGGITEPEAAEKLLIEGKADLIGVGRAILEDST
WAKRAIESLQ
>Mature_329_residues
SHLLKSLEIGPLSLKNRLVMPPMATAKAEPDGSVNQSIFDYYSEKSDGGYISLIIVEHSFISHEGQASEYQVSIADDQKI
DGLEKLAEIIQNNGSKAVMQINHAGSAAPEDIIGTKPVGPSAVPNPRKGNTPHELTEKEIQDIVEAFSNAARRTKEAGFD
GVEIHSAHGYLLNQFYSPLTNKRTDKYGGDVHKRIRIHLEVIEAVKKEVGEDFPILLRLGASDYQEGGTTIEDSKIAARE
FEKAGVDILDISGGHSGYMVNGLDGQGFFAPLTEAIKEVVSIPVILTGGITEPEAAEKLLIEGKADLIGVGRAILEDSTW
AKRAIESLQ

Specific function: Reduces a range of alternative electron acceptors [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the N-terminal section; belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1789463, Length=326, Percent_Identity=32.2085889570552, Blast_Score=168, Evalue=4e-43,
Organism=Escherichia coli, GI1787939, Length=340, Percent_Identity=30.8823529411765, Blast_Score=117, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17540738, Length=245, Percent_Identity=35.1020408163265, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17559802, Length=263, Percent_Identity=34.2205323193916, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17565138, Length=263, Percent_Identity=34.2205323193916, Blast_Score=139, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI17559804, Length=256, Percent_Identity=34.765625, Blast_Score=138, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI72001454, Length=321, Percent_Identity=29.9065420560748, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17564188, Length=256, Percent_Identity=32.421875, Blast_Score=130, Evalue=8e-31,
Organism=Caenorhabditis elegans, GI17566914, Length=308, Percent_Identity=28.5714285714286, Blast_Score=109, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17566916, Length=323, Percent_Identity=27.2445820433437, Blast_Score=84, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6321973, Length=233, Percent_Identity=34.3347639484979, Blast_Score=115, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6325086, Length=224, Percent_Identity=30.3571428571429, Blast_Score=108, Evalue=8e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013027
- InterPro:   IPR016040
- InterPro:   IPR001155
- InterPro:   IPR001327
- InterPro:   IPR000103 [H]

Pfam domain/function: PF00724 Oxidored_FMN; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.3.1.34 [C]

Molecular weight: Translated: 35699; Mature: 35567

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHLLKSLEIGPLSLKNRLVMPPMATAKAEPDGSVNQSIFDYYSEKSDGGYISLIIVEHS
CCHHHHHCCCCCCCCCCEEECCCCHHCCCCCCCCCHHHHHHHHHCCCCCCEEEEEEEEEH
FISHEGQASEYQVSIADDQKIDGLEKLAEIIQNNGSKAVMQINHAGSAAPEDIIGTKPVG
HHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCHHCCCCCCCC
PSAVPNPRKGNTPHELTEKEIQDIVEAFSNAARRTKEAGFDGVEIHSAHGYLLNQFYSPL
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHH
TNKRTDKYGGDVHKRIRIHLEVIEAVKKEVGEDFPILLRLGASDYQEGGTTIEDSKIAAR
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHCCCCEECCCHHHHH
EFEKAGVDILDISGGHSGYMVNGLDGQGFFAPLTEAIKEVVSIPVILTGGITEPEAAEKL
HHHHCCCEEEEECCCCCCCEEECCCCCCCHHHHHHHHHHHHHCCEEEECCCCCHHHHHHH
LIEGKADLIGVGRAILEDSTWAKRAIESLQ
HHCCCCCHHHHHHHHHCCCHHHHHHHHHCC
>Mature Secondary Structure 
SHLLKSLEIGPLSLKNRLVMPPMATAKAEPDGSVNQSIFDYYSEKSDGGYISLIIVEHS
CHHHHHCCCCCCCCCCEEECCCCHHCCCCCCCCCHHHHHHHHHCCCCCCEEEEEEEEEH
FISHEGQASEYQVSIADDQKIDGLEKLAEIIQNNGSKAVMQINHAGSAAPEDIIGTKPVG
HHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCHHCCCCCCCC
PSAVPNPRKGNTPHELTEKEIQDIVEAFSNAARRTKEAGFDGVEIHSAHGYLLNQFYSPL
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHH
TNKRTDKYGGDVHKRIRIHLEVIEAVKKEVGEDFPILLRLGASDYQEGGTTIEDSKIAAR
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHCCCCEECCCHHHHH
EFEKAGVDILDISGGHSGYMVNGLDGQGFFAPLTEAIKEVVSIPVILTGGITEPEAAEKL
HHHHCCCEEEEECCCCCCCEEECCCCCCCHHHHHHHHHHHHHCCEEEECCCCCHHHHHHH
LIEGKADLIGVGRAILEDSTWAKRAIESLQ
HHCCCCCHHHHHHHHHCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FAD. [C]

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: transDidehydroacyl-CoA; NADP+ [C]

Specific reaction: transDidehydroacyl-CoA + NADP+ =trans-transtetradehydroacyl-CoA + NADPH [C]

General reaction: Redox reaction [C]

Inhibitor: Iodoacetic acid; N-Ethyl maleimide [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8357835 [H]