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The map label for this gene is pps [H]

Identifier: 188585384

GI number: 188585384

Start: 779609

End: 782272

Strand: Direct

Name: pps [H]

Synonym: Nther_0751

Alternate gene names: 188585384

Gene position: 779609-782272 (Clockwise)

Preceding gene: 188585383

Following gene: 188585385

Centisome position: 24.63

GC content: 35.55

Gene sequence:

>2664_bases
ATGAACAATTATAAATTTATAAAATTGTTGGAGAATATCACAAAAGATGATCAATCACTAGTTGGAGGAAAGGGAGCTAA
TTTGGGTGAGATGATAAATGCCGGGCTTCCTGTACCAGGCGGTTTTGTATTACTAACAACTGCTTATAAAAGATTTGTTC
AGACCAATGGGTTACAAGAAAAAATTGATACCTTGATAAAGGGAATTGAAACCAGGGATGATAATTTAGCCGAGTTAACA
CAAATCTCAGAGGAAATCCAGAATTTATTTGAACAAGGTGATATACCTGAGGATATCCTGGAGGAAATAGAAAGTTCATA
TCAACAAATAGGAGAGCCAGAAGTGGCCGTTCGGTCATCTGCTACTTCTGAGGACCTGCCGGGAACATCTTTTGCAGGTC
AATATGAAACCTATTTGAATGTTTTGGGTAAAGAAGAACTCTGCAAATATATAAAAAAATGTTGGGCATCATTATGGAAC
CTAAGGGCGCTAAGCTATCGAATTAAACAAAAAACAAATATTACCGACTTAGCCCATGGTGTCGTTGTACAAAAACTAAT
TTATGCCGATAAATCAGGGATTTTATTTACAGCAAACCCAGTTAATGGACGTAGAGATCAAATGCTTCTTAACTCTTCCT
GGGGTTTAGGAGAGGCAATAGTAGGAGGGGAGGTGACACCGGATCAGTGGATTTTAGATAAGAATAACAATGAAATCGTT
ACAGAACAGATAGCAAAGAAAGAAATAATGACAATTAGACAAAATCAAGGTATTGATCATGTTAAAGTACCAGATGAACA
GCAAACTCAGGTTACACTAGATAATGAAGAAGTATTACAGTTATTGCAATTGGGAAGTCAGGTAGAAAATTACTATGGAT
TTCCTCAAGATATTGAGTGGGCATATTCAGATGACAAGTTTTATTTAGTACAAACACGGCCTATAACATCACTTTTCCCC
ATGCCTGAACCTTTAGATGAAGGGGATGATCTCCGGATATATTTAAATATGAGTTTATATTCTCAAGCTCTACACGAACC
TTTTACACCTATGGGTGAGGCTTTCTTTACAGGCATGTTTAAAAGCTTTGCTAAACGAATAAATAGGGATTATCAAAAGA
AACCTGTAAAGTGGTGCAAAACGGCCGGGGGTAGGCTATTTATAGATTTAACTGAGCTATTAAGATACGAAAGGGTCCGG
GAAAAGTTTAAAAATAATCCGGCTGATAAGGATCCTATAACAACTGAAGCTTTATTGCAGGTTGTAGAAAGAAATAAAGA
TGAACTGATCAAGAACAAAAAATCACCCATAAGTGTGGCATTTAAAATGATTTCAAAAATGAACCCCTGGGTAATTAAAT
TTTTGTTAACTTCCATTCATAAAGTTATGTACGGAGTAGTTTCTCCTGATAAAGCTGTTACAAAAGCTTTTGAGTACGGG
CAAAGTCAAATAGCAAATATTGAACAGTCCAGAAATCAGTTAACAGAGATTGAAGATAAACTAAGGTATATAGAGGAAGT
TGCTACTCGAGACATTTTTGTTGGCTTGTTTGAAGTAGTTTTTTATGTATCAGCTTCAACGAACTATATTAAAAAAGCTA
AAGCCATATTAAAAAAATACAATGAGGACACATCACAACTAGACAAAGTAGAGCAATCTGTTCCCAATAATGTTACTACT
GAAATGGGTATGGAATTATTAAATATTGCAAAAGAATTAGGTGAAAATGGTGAACAACCAGATGCTGATCATCCACAGAT
CCGAGAGTTTTTAAATAAGTACGGTCATAGATCTTCAAACGAAGTTGATATAGGTATCCCCAGATGGTATGAAAAGCCTC
AGTATGTTGTAGATCTAGTGCAAGCTTATATGGACAATAAAAGCTATGATGATGGGATATCCAAATTTTATCAGGGTATG
GAAGAGGCCCAACAAGTAATTGGAAATATAACAAATAGATTAAGAGAACAAGGTGGTCATAAGGATGCAAAAAAAGTTAA
AAAACTATTAGAAAACTACCGAAAAATGTTTGGTGTTAGGGAACTACCAAAATTTTATTTAACAAAAGCTTTCAGCATCT
TAAGAGAAATGTTAAAAGATATCGGTACAGAACTGCAACAACAAGGGAAATTAGACGACAAACTGGACATTTTCTTTGTT
AATTTCCATGATCTCAAATCTATGGAAAATTTAAAGGAAATTGTAACAGAAAACAAGGAAAAATATTTCAGAGAAATGCA
AAGGCCTTCTGTCCCTAGGATTATGACAAATACAGGTGAATCTATTTATTCAGCAGCAGTAGACAATGAAAGTGAAGGTG
TATATAGTGGAGTGCCTGTGTCTCCCGGGGTTATTGAAGGAGTTGTCAGAGTAATAAATCACCCAGAAGAAGGAAGCAAA
TTGAAGAAAGGTGAAATTTTAGTAACAAAAGGTACCAATCCAGCTTGGACACCATTATTTCTTAAAATTAGTGGCTTAAT
TATGGAAACAGGTGGACCTATTTCCCATGGCTCTGTTGTAGCAAGAGAGTACGGTTTACCAGCAGTAGCCGGTGTTAGTG
CTGCTACAGATAGATTGGTAGATGGACAAAAAGTAAGACTAAATGGAGAAACTGGGAATGTAGAAATATTAGAGGATCCC
GAATCACATGCTAGTGATAAATAA

Upstream 100 bases:

>100_bases
AAAGTGTAGAGTATTATCGGTAAATCGGAAATAGGAAGCTTTTTAGCATTGCGACATACCGTCAGTCGGTATGCCGACAA
TCAAAAGGAGGAAATCTCCC

Downstream 100 bases:

>100_bases
ATTCAGAAAGATATTCCTTTGTTAAACTACCTGGATTACTCAGATAAAGCCTGTAAGTTGCCTCTTCATCATAATGAGAA
ATATCCGTATAGGAAAAATA

Product: pyruvate phosphate dikinase PEP/pyruvate-binding

Products: AMP; phosphoenolpyruvate; phosphate

Alternate protein names: Putative PEP synthase; Pyruvate, water dikinase [H]

Number of amino acids: Translated: 887; Mature: 887

Protein sequence:

>887_residues
MNNYKFIKLLENITKDDQSLVGGKGANLGEMINAGLPVPGGFVLLTTAYKRFVQTNGLQEKIDTLIKGIETRDDNLAELT
QISEEIQNLFEQGDIPEDILEEIESSYQQIGEPEVAVRSSATSEDLPGTSFAGQYETYLNVLGKEELCKYIKKCWASLWN
LRALSYRIKQKTNITDLAHGVVVQKLIYADKSGILFTANPVNGRRDQMLLNSSWGLGEAIVGGEVTPDQWILDKNNNEIV
TEQIAKKEIMTIRQNQGIDHVKVPDEQQTQVTLDNEEVLQLLQLGSQVENYYGFPQDIEWAYSDDKFYLVQTRPITSLFP
MPEPLDEGDDLRIYLNMSLYSQALHEPFTPMGEAFFTGMFKSFAKRINRDYQKKPVKWCKTAGGRLFIDLTELLRYERVR
EKFKNNPADKDPITTEALLQVVERNKDELIKNKKSPISVAFKMISKMNPWVIKFLLTSIHKVMYGVVSPDKAVTKAFEYG
QSQIANIEQSRNQLTEIEDKLRYIEEVATRDIFVGLFEVVFYVSASTNYIKKAKAILKKYNEDTSQLDKVEQSVPNNVTT
EMGMELLNIAKELGENGEQPDADHPQIREFLNKYGHRSSNEVDIGIPRWYEKPQYVVDLVQAYMDNKSYDDGISKFYQGM
EEAQQVIGNITNRLREQGGHKDAKKVKKLLENYRKMFGVRELPKFYLTKAFSILREMLKDIGTELQQQGKLDDKLDIFFV
NFHDLKSMENLKEIVTENKEKYFREMQRPSVPRIMTNTGESIYSAAVDNESEGVYSGVPVSPGVIEGVVRVINHPEEGSK
LKKGEILVTKGTNPAWTPLFLKISGLIMETGGPISHGSVVAREYGLPAVAGVSAATDRLVDGQKVRLNGETGNVEILEDP
ESHASDK

Sequences:

>Translated_887_residues
MNNYKFIKLLENITKDDQSLVGGKGANLGEMINAGLPVPGGFVLLTTAYKRFVQTNGLQEKIDTLIKGIETRDDNLAELT
QISEEIQNLFEQGDIPEDILEEIESSYQQIGEPEVAVRSSATSEDLPGTSFAGQYETYLNVLGKEELCKYIKKCWASLWN
LRALSYRIKQKTNITDLAHGVVVQKLIYADKSGILFTANPVNGRRDQMLLNSSWGLGEAIVGGEVTPDQWILDKNNNEIV
TEQIAKKEIMTIRQNQGIDHVKVPDEQQTQVTLDNEEVLQLLQLGSQVENYYGFPQDIEWAYSDDKFYLVQTRPITSLFP
MPEPLDEGDDLRIYLNMSLYSQALHEPFTPMGEAFFTGMFKSFAKRINRDYQKKPVKWCKTAGGRLFIDLTELLRYERVR
EKFKNNPADKDPITTEALLQVVERNKDELIKNKKSPISVAFKMISKMNPWVIKFLLTSIHKVMYGVVSPDKAVTKAFEYG
QSQIANIEQSRNQLTEIEDKLRYIEEVATRDIFVGLFEVVFYVSASTNYIKKAKAILKKYNEDTSQLDKVEQSVPNNVTT
EMGMELLNIAKELGENGEQPDADHPQIREFLNKYGHRSSNEVDIGIPRWYEKPQYVVDLVQAYMDNKSYDDGISKFYQGM
EEAQQVIGNITNRLREQGGHKDAKKVKKLLENYRKMFGVRELPKFYLTKAFSILREMLKDIGTELQQQGKLDDKLDIFFV
NFHDLKSMENLKEIVTENKEKYFREMQRPSVPRIMTNTGESIYSAAVDNESEGVYSGVPVSPGVIEGVVRVINHPEEGSK
LKKGEILVTKGTNPAWTPLFLKISGLIMETGGPISHGSVVAREYGLPAVAGVSAATDRLVDGQKVRLNGETGNVEILEDP
ESHASDK
>Mature_887_residues
MNNYKFIKLLENITKDDQSLVGGKGANLGEMINAGLPVPGGFVLLTTAYKRFVQTNGLQEKIDTLIKGIETRDDNLAELT
QISEEIQNLFEQGDIPEDILEEIESSYQQIGEPEVAVRSSATSEDLPGTSFAGQYETYLNVLGKEELCKYIKKCWASLWN
LRALSYRIKQKTNITDLAHGVVVQKLIYADKSGILFTANPVNGRRDQMLLNSSWGLGEAIVGGEVTPDQWILDKNNNEIV
TEQIAKKEIMTIRQNQGIDHVKVPDEQQTQVTLDNEEVLQLLQLGSQVENYYGFPQDIEWAYSDDKFYLVQTRPITSLFP
MPEPLDEGDDLRIYLNMSLYSQALHEPFTPMGEAFFTGMFKSFAKRINRDYQKKPVKWCKTAGGRLFIDLTELLRYERVR
EKFKNNPADKDPITTEALLQVVERNKDELIKNKKSPISVAFKMISKMNPWVIKFLLTSIHKVMYGVVSPDKAVTKAFEYG
QSQIANIEQSRNQLTEIEDKLRYIEEVATRDIFVGLFEVVFYVSASTNYIKKAKAILKKYNEDTSQLDKVEQSVPNNVTT
EMGMELLNIAKELGENGEQPDADHPQIREFLNKYGHRSSNEVDIGIPRWYEKPQYVVDLVQAYMDNKSYDDGISKFYQGM
EEAQQVIGNITNRLREQGGHKDAKKVKKLLENYRKMFGVRELPKFYLTKAFSILREMLKDIGTELQQQGKLDDKLDIFFV
NFHDLKSMENLKEIVTENKEKYFREMQRPSVPRIMTNTGESIYSAAVDNESEGVYSGVPVSPGVIEGVVRVINHPEEGSK
LKKGEILVTKGTNPAWTPLFLKISGLIMETGGPISHGSVVAREYGLPAVAGVSAATDRLVDGQKVRLNGETGNVEILEDP
ESHASDK

Specific function: Might catalyze the phosphorylation of pyruvate to phosphoenolpyruvate (Potential) [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1787994, Length=324, Percent_Identity=35.4938271604938, Blast_Score=177, Evalue=3e-45,
Organism=Caenorhabditis elegans, GI17564524, Length=355, Percent_Identity=29.5774647887324, Blast_Score=128, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR002192 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF01326 PPDK_N [H]

EC number: 2.7.9.2

Molecular weight: Translated: 100508; Mature: 100508

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNYKFIKLLENITKDDQSLVGGKGANLGEMINAGLPVPGGFVLLTTAYKRFVQTNGLQE
CCCCHHHHHHHHHCCCHHHHCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCHHH
KIDTLIKGIETRDDNLAELTQISEEIQNLFEQGDIPEDILEEIESSYQQIGEPEVAVRSS
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEEHCCC
ATSEDLPGTSFAGQYETYLNVLGKEELCKYIKKCWASLWNLRALSYRIKQKTNITDLAHG
CCCCCCCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
VVVQKLIYADKSGILFTANPVNGRRDQMLLNSSWGLGEAIVGGEVTPDQWILDKNNNEIV
HHHHHHHHHCCCCEEEECCCCCCCHHHEEECCCCCCCCCCCCCCCCCCCEEEECCCCCHH
TEQIAKKEIMTIRQNQGIDHVKVPDEQQTQVTLDNEEVLQLLQLGSQVENYYGFPQDIEW
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHCCCCCCCCE
AYSDDKFYLVQTRPITSLFPMPEPLDEGDDLRIYLNMSLYSQALHEPFTPMGEAFFTGMF
EECCCEEEEEECCCCHHHCCCCCCCCCCCCEEEEEEHHHHHHHHHCCCCCHHHHHHHHHH
KSFAKRINRDYQKKPVKWCKTAGGRLFIDLTELLRYERVREKFKNNPADKDPITTEALLQ
HHHHHHHCCHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHH
VVERNKDELIKNKKSPISVAFKMISKMNPWVIKFLLTSIHKVMYGVVSPDKAVTKAFEYG
HHHCCHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
QSQIANIEQSRNQLTEIEDKLRYIEEVATRDIFVGLFEVVFYVSASTNYIKKAKAILKKY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
NEDTSQLDKVEQSVPNNVTTEMGMELLNIAKELGENGEQPDADHPQIREFLNKYGHRSSN
CCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCC
EVDIGIPRWYEKPQYVVDLVQAYMDNKSYDDGISKFYQGMEEAQQVIGNITNRLREQGGH
CEECCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
KDAKKVKKLLENYRKMFGVRELPKFYLTKAFSILREMLKDIGTELQQQGKLDDKLDIFFV
CHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEE
NFHDLKSMENLKEIVTENKEKYFREMQRPSVPRIMTNTGESIYSAAVDNESEGVYSGVPV
EHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHCCCHHHHHHHCCCCCCCCCCCCCC
SPGVIEGVVRVINHPEEGSKLKKGEILVTKGTNPAWTPLFLKISGLIMETGGPISHGSVV
CCHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCCCHHHEEEEEEHEECCCCCCCCCHH
AREYGLPAVAGVSAATDRLVDGQKVRLNGETGNVEILEDPESHASDK
HHHCCCCHHHCCHHHHHHHCCCCEEEEECCCCCEEEEECCHHCCCCC
>Mature Secondary Structure
MNNYKFIKLLENITKDDQSLVGGKGANLGEMINAGLPVPGGFVLLTTAYKRFVQTNGLQE
CCCCHHHHHHHHHCCCHHHHCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCHHH
KIDTLIKGIETRDDNLAELTQISEEIQNLFEQGDIPEDILEEIESSYQQIGEPEVAVRSS
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEEHCCC
ATSEDLPGTSFAGQYETYLNVLGKEELCKYIKKCWASLWNLRALSYRIKQKTNITDLAHG
CCCCCCCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
VVVQKLIYADKSGILFTANPVNGRRDQMLLNSSWGLGEAIVGGEVTPDQWILDKNNNEIV
HHHHHHHHHCCCCEEEECCCCCCCHHHEEECCCCCCCCCCCCCCCCCCCEEEECCCCCHH
TEQIAKKEIMTIRQNQGIDHVKVPDEQQTQVTLDNEEVLQLLQLGSQVENYYGFPQDIEW
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHCCCCCCCCE
AYSDDKFYLVQTRPITSLFPMPEPLDEGDDLRIYLNMSLYSQALHEPFTPMGEAFFTGMF
EECCCEEEEEECCCCHHHCCCCCCCCCCCCEEEEEEHHHHHHHHHCCCCCHHHHHHHHHH
KSFAKRINRDYQKKPVKWCKTAGGRLFIDLTELLRYERVREKFKNNPADKDPITTEALLQ
HHHHHHHCCHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHH
VVERNKDELIKNKKSPISVAFKMISKMNPWVIKFLLTSIHKVMYGVVSPDKAVTKAFEYG
HHHCCHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
QSQIANIEQSRNQLTEIEDKLRYIEEVATRDIFVGLFEVVFYVSASTNYIKKAKAILKKY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
NEDTSQLDKVEQSVPNNVTTEMGMELLNIAKELGENGEQPDADHPQIREFLNKYGHRSSN
CCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCC
EVDIGIPRWYEKPQYVVDLVQAYMDNKSYDDGISKFYQGMEEAQQVIGNITNRLREQGGH
CEECCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
KDAKKVKKLLENYRKMFGVRELPKFYLTKAFSILREMLKDIGTELQQQGKLDDKLDIFFV
CHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEE
NFHDLKSMENLKEIVTENKEKYFREMQRPSVPRIMTNTGESIYSAAVDNESEGVYSGVPV
EHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHCCCHHHHHHHCCCCCCCCCCCCCC
SPGVIEGVVRVINHPEEGSKLKKGEILVTKGTNPAWTPLFLKISGLIMETGGPISHGSVV
CCHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCCCHHHEEEEEEHEECCCCCCCCCHH
AREYGLPAVAGVSAATDRLVDGQKVRLNGETGNVEILEDPESHASDK
HHHCCCCHHHCCHHHHHHHCCCCEEEEECCCCCEEEEECCHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Mg2+; Mn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 10.5 {phosphate}} 0.083 {pyruvate}} 0.028 {ATP}} [C]

Substrates: ATP; pyruvate; H2O

Specific reaction: ATP + pyruvate + H2O = AMP + phosphoenolpyruvate + phosphate

General reaction: Phospho group transfer [C]

Inhibitor: 2-Oxoglutarate; 3-Phosphoglyceraldehyde; 5'-Adenylyl methylen ediphosphonate; ADP; ADP glucose; AMP; ATP; Ca2+; F-; Iodoacetate; Malate; Mg2+; Mn2+; Oxalacetate; PCMB; Phosphoenolpyruvate; Sulfhydryl reagents [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 7704256 [H]