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The map label for this gene is mleN [H]

Identifier: 188585369

GI number: 188585369

Start: 763142

End: 764545

Strand: Direct

Name: mleN [H]

Synonym: Nther_0736

Alternate gene names: 188585369

Gene position: 763142-764545 (Clockwise)

Preceding gene: 188585362

Following gene: 188585371

Centisome position: 24.11

GC content: 36.61

Gene sequence:

>1404_bases
ATGAACGAAAGTAATCAGAATTTTAAAAAGCCTTCTGCTGGACAAGCTTTCACACCTTTAATTGCACTAATAGTATTAGT
TGCTTATGCTGCTATTTTCTTGGGAGCAGATGTGCATATTCCGCTTTTTATTGCAGTTGTGATTACAGCTATAGTAGCTG
TTGCCTGGATTGGCCATGAATGGAAGGTAGTTCAAGATGGTATGATTAAAGGGATTAATTTTGCCCTTGATGCAGTAATA
ATTTTATCTATTGTTGGAATTTTAGTTGGTAGTTGGGTTGAAGGTGGAATTGTACCTACAATAGTTTACTACGGTTTACA
AATGATTTCACCACAAATATTTTTAATTGCAACACTTATTATTTGTGCACTAGTATCAATATTTACTGGAAGTTCATGGA
GTTCTCTTGCTACGGTTGGTTTAGCTATGTTTGGAATTGGTGAAGGTCTTGGTATACCAGGTGGTATGACTGTAGGTGCT
ATTGTATCTGGAGCTTACTTTGGAGATAAATTATCTCCACTTTCTGATACTACTGTTGTAGCTTCAGGTGCTGCAGGAGT
AAATATTTATGATCACATTAAGCATATGTTATGGGTATCAGGTCCGGGATTTGTAATTTCTCTTATAGTTTTTGGTATAT
TTGGTTTACAGTATGCAGGATCAGATTTGGATGCTGATAGAATTAGTGAAATTACTGGAGCTATTTCAGGCAGCTTTAAT
GTAAGTCCAATAATGTTTATACCTTTAATTGTAGTACTAGTGATGATTCTTATGAGAGTTCCCCCGATACCTGCTTTAGT
AGGTGGTTGTTTCTTGGGTCTTATTTGGGCTATGGTATTTCAGGGATCTTCATTCGCCGATGTATTAGGGTCAATGAACT
ATGGATTTGTCATGGAAAGTAACGTAGAACTTGTCGATGACTTGTTTACTACCGGTGGACTTCAAAGTATGATGTGGACT
ATATCACTAATATTAATAGCTTTGACCTTTGGTGGAATAATTGAGCATACGAAAATGATGGAAGTTATTCTGGAAAAGAT
CCTCAATATAGCGACTACAAAAAGATCTCTAATAATTGTTTCTCACATTACCACATTGATTCTAATTTTGACTGTACTTT
CTCACTATTTAACCCACGTACTAACTTGTAGAGCATACAAGCACGCCTTTGAAGAAAGAGGACTACACGCTAAAAACGTT
TCCCGTATCTCTGAAGCCTGGGGTACTCTACCTTCTTCCTTAATTCCATGGGGACCATGTGGTGCTTATGTATTTGGTCT
TTTTGGAGTACCTGTAATAGAGTATTTCCCATTTGCAATTTATATTCTTGCAACAATGGTCGTTTCGATGGTATTTGCCA
TTGTAGGTTTCAGTATGGAACCATATGAGAAAACAGAGGCTTAA

Upstream 100 bases:

>100_bases
AAACTAGATGATTTTTACAATCCTTTGTAACAGATTCTGTAACATTAAGCTTGGTGAACTTGGTAATTATTTCAGAACAT
ACCATTTAGGAGGGATATTT

Downstream 100 bases:

>100_bases
AAATTAATAAAATAGTTTAGATTTTAGGAAGTTGATCTTCTTTAAAAGTAAGTATCTTTTAAAGAAGATTGAAGGGCTAT
CATAACTGGATGGCCCTTCT

Product: Na+/H+ antiporter NhaC

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 467; Mature: 467

Protein sequence:

>467_residues
MNESNQNFKKPSAGQAFTPLIALIVLVAYAAIFLGADVHIPLFIAVVITAIVAVAWIGHEWKVVQDGMIKGINFALDAVI
ILSIVGILVGSWVEGGIVPTIVYYGLQMISPQIFLIATLIICALVSIFTGSSWSSLATVGLAMFGIGEGLGIPGGMTVGA
IVSGAYFGDKLSPLSDTTVVASGAAGVNIYDHIKHMLWVSGPGFVISLIVFGIFGLQYAGSDLDADRISEITGAISGSFN
VSPIMFIPLIVVLVMILMRVPPIPALVGGCFLGLIWAMVFQGSSFADVLGSMNYGFVMESNVELVDDLFTTGGLQSMMWT
ISLILIALTFGGIIEHTKMMEVILEKILNIATTKRSLIIVSHITTLILILTVLSHYLTHVLTCRAYKHAFEERGLHAKNV
SRISEAWGTLPSSLIPWGPCGAYVFGLFGVPVIEYFPFAIYILATMVVSMVFAIVGFSMEPYEKTEA

Sequences:

>Translated_467_residues
MNESNQNFKKPSAGQAFTPLIALIVLVAYAAIFLGADVHIPLFIAVVITAIVAVAWIGHEWKVVQDGMIKGINFALDAVI
ILSIVGILVGSWVEGGIVPTIVYYGLQMISPQIFLIATLIICALVSIFTGSSWSSLATVGLAMFGIGEGLGIPGGMTVGA
IVSGAYFGDKLSPLSDTTVVASGAAGVNIYDHIKHMLWVSGPGFVISLIVFGIFGLQYAGSDLDADRISEITGAISGSFN
VSPIMFIPLIVVLVMILMRVPPIPALVGGCFLGLIWAMVFQGSSFADVLGSMNYGFVMESNVELVDDLFTTGGLQSMMWT
ISLILIALTFGGIIEHTKMMEVILEKILNIATTKRSLIIVSHITTLILILTVLSHYLTHVLTCRAYKHAFEERGLHAKNV
SRISEAWGTLPSSLIPWGPCGAYVFGLFGVPVIEYFPFAIYILATMVVSMVFAIVGFSMEPYEKTEA
>Mature_467_residues
MNESNQNFKKPSAGQAFTPLIALIVLVAYAAIFLGADVHIPLFIAVVITAIVAVAWIGHEWKVVQDGMIKGINFALDAVI
ILSIVGILVGSWVEGGIVPTIVYYGLQMISPQIFLIATLIICALVSIFTGSSWSSLATVGLAMFGIGEGLGIPGGMTVGA
IVSGAYFGDKLSPLSDTTVVASGAAGVNIYDHIKHMLWVSGPGFVISLIVFGIFGLQYAGSDLDADRISEITGAISGSFN
VSPIMFIPLIVVLVMILMRVPPIPALVGGCFLGLIWAMVFQGSSFADVLGSMNYGFVMESNVELVDDLFTTGGLQSMMWT
ISLILIALTFGGIIEHTKMMEVILEKILNIATTKRSLIIVSHITTLILILTVLSHYLTHVLTCRAYKHAFEERGLHAKNV
SRISEAWGTLPSSLIPWGPCGAYVFGLFGVPVIEYFPFAIYILATMVVSMVFAIVGFSMEPYEKTEA

Specific function: Couples proton uptake and Na(+) efflux to the substrate- product malate/lactate antiport, in an electroneutral malate- 2H(+)/Na(+)-lactate exchange. Plays a role in supporting growth to high density on malate at reduced protonmotive force [H]

COG id: COG1757

COG function: function code C; Na+/H+ antiporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nhaC Na(+)/H(+) antiporter family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004770
- InterPro:   IPR018461 [H]

Pfam domain/function: PF03553 Na_H_antiporter [H]

EC number: NA

Molecular weight: Translated: 49998; Mature: 49998

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377; 10903309 [H]