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The map label for this gene is opuD [H]

Identifier: 188585246

GI number: 188585246

Start: 633491

End: 634963

Strand: Reverse

Name: opuD [H]

Synonym: Nther_0608

Alternate gene names: 188585246

Gene position: 634963-633491 (Counterclockwise)

Preceding gene: 188585247

Following gene: 188585242

Centisome position: 20.06

GC content: 39.78

Gene sequence:

>1473_bases
ATGAAAATGCAAACAGTATTTATAGTTTCCCTGGCCATTACATTAATAGTTCTGGGTTTGGGTATTTTCAATCAACCAGC
CTTGGAATACTATGTGGAAACGGCCTACCAAGGCATTCTCGAATATTTTGGCTGGGTTTACATGTTAGCCACCTTTTTCT
TTTTGGTTTTTGCCATAGGCCTTTCATTTAGTAGGTTTGGGCAGATCAAACTAGGAGCTGATCATGAAAAACCTCAGTAT
AGTTACTTTGGCTGGTTTAGTATGCTATTTGCAGCTGGAATGGGAATTGGGTTAATTTTTTGGGGTGTAAGTGAACCTCT
GGTTCATTATCTTGAACCCCCGGCCCATATTCCAGAAGCTTCTGGGGATGCTGCTGGCTTTGCCATGAGGTACAGTTTTT
TCCACTGGGGATTACAGCCATGGGCTATCTACATTATTATGAGCCTGTCAATTGCCTATTTTTCCTTTAGGAGAGGGATG
CCTCCCCTAATCAGTTCGTGCTTCTATCCCATGTTGGGAGAAAACATATACAAATTCCCGGGATACTTGATTGACATTTT
AGCCGTAGTTGCAACTATATTTGGTGTGGCCACCTCCTTGGGATTGGGAGCTACACAAATTCATAGTGGTTTAAGCTTTC
TCTACGGATTACCCGATGGTTTTAACATTACTTTAATAATCGTAATAATAGCTACAATTTTATATATGATTTCCAGTGTA
GTAGGATTGGACAAGGGTATTCAAGCATTAAGTAAATTAAATATGTTTATTGCCTTAATTTTGATGGTATTCATGTTAAT
AATGGGTCCCACCTCTTATATCTTTAACATATTTACAAGTACCATCGGAACATATACTAACCGTTTACTTGAGATGAGCC
TTGATACTAACCCTTTTACTGGACATGAATGGATAGAATCCTGGACTCTTTTCTATTGGGCCTGGTGGATTTCCTGGTCA
CCTTTTGTCGGTTTATTTGTAGCGAGAATTTCCAGAGGTAGGACTATTAAGGAATTTATCCACGGAGTGTTGCTGGTACC
AACCCTGATGACATTTTTATGGTTCAGCGTATTCGGTGGAGCCGGTTTTCAACTACAATTGACCGAAGGTATCCATATAG
CGGAAATTGCTCAACAGGACGCTTCCCTGGCATTATTCCACTTATTTGAAGCTTTCCCATTGGGAAGTATTTTATCGTCA
GTCACTATCCTGTTACTATTTGTGTTTTTCATTACTAGTGCAGACTCGGCAACCTTCGTCCTTGGTATTTTAAGCTCAAA
TGGTACTCTCAATCCATCACTAGCTAAAAAGATGACCTGGGGAGCTAGCCAGTCAGGGATAGCTCTTATCCTGTTATTCA
CAGGTGGAATTCAAGCTCTACAACAAATGTCAATAACAGCAGCTCTACCTTTTTCAATTATTATGGTCTTGTTATGCTAT
AACCTATTGTCAGCCTTGAAAGAAGAAGTTTAG

Upstream 100 bases:

>100_bases
TGGGGAAATTATCATGGGTACAACTGCTGAACGAGTTTTAAAAGGAACCCAGTCTTCAGTTTTAATTATTCCGTAAATAA
CTTAAATTGGGGTGATCAGA

Downstream 100 bases:

>100_bases
TCAACGAACTATACAGCACTTTTTATAGACTGTATTAAAGTTAAATGTTAATAACTGTATAACAACACCTTTTTATAAGG
ACTGAAGCAGTTTCTTGACC

Product: choline/carnitine/betaine transporter

Products: Proton [Cytoplasm]; choline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 490; Mature: 490

Protein sequence:

>490_residues
MKMQTVFIVSLAITLIVLGLGIFNQPALEYYVETAYQGILEYFGWVYMLATFFFLVFAIGLSFSRFGQIKLGADHEKPQY
SYFGWFSMLFAAGMGIGLIFWGVSEPLVHYLEPPAHIPEASGDAAGFAMRYSFFHWGLQPWAIYIIMSLSIAYFSFRRGM
PPLISSCFYPMLGENIYKFPGYLIDILAVVATIFGVATSLGLGATQIHSGLSFLYGLPDGFNITLIIVIIATILYMISSV
VGLDKGIQALSKLNMFIALILMVFMLIMGPTSYIFNIFTSTIGTYTNRLLEMSLDTNPFTGHEWIESWTLFYWAWWISWS
PFVGLFVARISRGRTIKEFIHGVLLVPTLMTFLWFSVFGGAGFQLQLTEGIHIAEIAQQDASLALFHLFEAFPLGSILSS
VTILLLFVFFITSADSATFVLGILSSNGTLNPSLAKKMTWGASQSGIALILLFTGGIQALQQMSITAALPFSIIMVLLCY
NLLSALKEEV

Sequences:

>Translated_490_residues
MKMQTVFIVSLAITLIVLGLGIFNQPALEYYVETAYQGILEYFGWVYMLATFFFLVFAIGLSFSRFGQIKLGADHEKPQY
SYFGWFSMLFAAGMGIGLIFWGVSEPLVHYLEPPAHIPEASGDAAGFAMRYSFFHWGLQPWAIYIIMSLSIAYFSFRRGM
PPLISSCFYPMLGENIYKFPGYLIDILAVVATIFGVATSLGLGATQIHSGLSFLYGLPDGFNITLIIVIIATILYMISSV
VGLDKGIQALSKLNMFIALILMVFMLIMGPTSYIFNIFTSTIGTYTNRLLEMSLDTNPFTGHEWIESWTLFYWAWWISWS
PFVGLFVARISRGRTIKEFIHGVLLVPTLMTFLWFSVFGGAGFQLQLTEGIHIAEIAQQDASLALFHLFEAFPLGSILSS
VTILLLFVFFITSADSATFVLGILSSNGTLNPSLAKKMTWGASQSGIALILLFTGGIQALQQMSITAALPFSIIMVLLCY
NLLSALKEEV
>Mature_490_residues
MKMQTVFIVSLAITLIVLGLGIFNQPALEYYVETAYQGILEYFGWVYMLATFFFLVFAIGLSFSRFGQIKLGADHEKPQY
SYFGWFSMLFAAGMGIGLIFWGVSEPLVHYLEPPAHIPEASGDAAGFAMRYSFFHWGLQPWAIYIIMSLSIAYFSFRRGM
PPLISSCFYPMLGENIYKFPGYLIDILAVVATIFGVATSLGLGATQIHSGLSFLYGLPDGFNITLIIVIIATILYMISSV
VGLDKGIQALSKLNMFIALILMVFMLIMGPTSYIFNIFTSTIGTYTNRLLEMSLDTNPFTGHEWIESWTLFYWAWWISWS
PFVGLFVARISRGRTIKEFIHGVLLVPTLMTFLWFSVFGGAGFQLQLTEGIHIAEIAQQDASLALFHLFEAFPLGSILSS
VTILLLFVFFITSADSATFVLGILSSNGTLNPSLAKKMTWGASQSGIALILLFTGGIQALQQMSITAALPFSIIMVLLCY
NLLSALKEEV

Specific function: High-affinity uptake of glycine betaine [H]

COG id: COG1292

COG function: function code M; Choline-glycine betaine transporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the BCCT transporter (TC 2.A.15) family [H]

Homologues:

Organism=Escherichia coli, GI1786506, Length=454, Percent_Identity=40.5286343612335, Blast_Score=348, Evalue=3e-97,
Organism=Escherichia coli, GI1788102, Length=435, Percent_Identity=31.0344827586207, Blast_Score=224, Evalue=8e-60,
Organism=Escherichia coli, GI1786224, Length=496, Percent_Identity=28.0241935483871, Blast_Score=199, Evalue=4e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000060
- InterPro:   IPR018093 [H]

Pfam domain/function: PF02028 BCCT [H]

EC number: NA

Molecular weight: Translated: 54400; Mature: 54400

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: PS01303 BCCT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHCC
>Mature Secondary Structure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HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; choline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + choline [Periplasm] = Proton [Cytoplasm] + choline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8752321; 9387221; 9384377 [H]