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The map label for this gene is pyk [H]

Identifier: 188585189

GI number: 188585189

Start: 573630

End: 575372

Strand: Direct

Name: pyk [H]

Synonym: Nther_0550

Alternate gene names: 188585189

Gene position: 573630-575372 (Clockwise)

Preceding gene: 188585188

Following gene: 188585190

Centisome position: 18.12

GC content: 39.93

Gene sequence:

>1743_bases
ATGTTAAGAAAAACCAAAATTGTGGCAACTATAGGCCCTGCTTGTGAAAACAAAGAAAGTTTAGCCAGGATGGCCCAAAA
CGGAATGGATGTCTGTCGGTTAAATTTTTCACATGGTGAACACCAGGAGCACCTGGATAGGATAAATTTAATCAGGGAAG
TTTATCAGGAATTAAAGTTTCCCCCAGCTATTTTACTTGATACCAGAGGTCCTGAAATCAGATTGGGAACTTTTGAAAAT
GGTGAAGTGGATTTGGAAAAAGGACAAGAATTTACTTTATCAACTGAAGAAATAGAAGGGGATACTACAAGAGTACATGT
AAATTATCCACAAGTCACTTCTGATATAAGTCCCGGAGATAAGGTGCTTCTTGATGATGGTTTGATTGAGCTATTAGTTA
ATGAAGTGACTGAAACAGAGGTTAAAACAGTCGTCAATAATGACGGTACCATTAAGGATAATAAAGGTGTCAATTTACCA
GGTGTGACTCTATCTTTACCAGCTATGACTGACAAAGACCAACAAGATATTTTATTTGGTATCGAAAACCAGGTGGATTT
TATTGCAGCTTCATTTATTAGAAAGCCAGAAGATGTTTTAGAAATTAGAAAATTCCTTGAAGAACATGGTTCAGATATTC
CCGTAATATCAAAAATTGAGAATAAAGAAGGTGTTGAAAATTTAGAAAGAATCTTAGAAGTTTCCGATGGCCTAATGGTG
GCACGAGGAGACCTGGGTGTTGATATACCACCAGAAGAAGTGCCCTTAGTGCAAAAAAGAATGATTAACAGATGTAATGA
TGAAGGTAAGCCTGTTATAACAGCTACACAAATGCTAGAGTCCATGACGGAAAACCCACGCCCAACTAGGGCAGAAGCCA
GCGACGTTGCTAATGCTATCATAGACGGAACTGATGCGGTTATGCTTAGTGGAGAAACGGCTGCTGGAGCTTACCCCGTG
GAATCGGTAAGTACTATGGCAAATATAGCAAAAAGTACGGAGAGGGAGCTATCTAGAAAACCACAAGAAGTTACCGCTAA
AGACACTGGTATGACCGAAGCTATTGGGTACTCCACCTGTCAGATAGCAGGCCAATTAGAAGCTAATGCCATATTAACAG
CTACCCAATCTGGGTATACGGCAAGAATGGTTGCCAAATACAAACCAAAAGCGCCAATTGTGGCAGTGACGCCAATTGAA
AAAGTTGCCAGGAGATTATCTTTATCATGGGGTGTTTATCCAATAACCTGTGACCCTTCAGAAACAACAGATGAAATGTT
TCAGGAAGCAATTCAAACAGCCCTGATAACTCAATATATTAGTGAAGGGGACCTTATTGTTATAACAGCAGGAATTCCAG
TAGGAGTTTCGGGAACAACTAACTTAATTAGAGTACATACTGTGGGAGAAATCTTAGTTTCAGGTGCAGGAATCGGGAAA
AAACCGGCAACTGGCAAAGTGGTCAAAGTAGAAGATAGCAATGATTTATCTAAAATTTCTAGTGGTGATATAGTAGTGTT
GCGGGGTACTACAGATGAAATGGTTCCTCATCTGGCTCAGGTTAGTGGTCTTGTTGCCGAAGAAGGTGGTTACACATCAC
CAAGTGCAATTGTAGGACTGGATCTAGGATTACCTGTTATTGTTGGTGCTGAAGGAGCTTTAGAAAAACTGGCAGATGGT
GATCAGGTTACTATAGATCCTTTACGAGGTTTTGTCTATAAAGGGCAAGCAACCATATTGTAA

Upstream 100 bases:

>100_bases
TCCAAGAAATAATGGGAACTGATTCTATTAAATTAAAATATTCACTGTAAGAAAATACGTGCCCTTTTTTAATTTAGTAA
ATAAAAGGAGGAAACACTCA

Downstream 100 bases:

>100_bases
ACCATGAGTAGATTAGAGGTGAGTTAAATGATTTCAAAAACAAATATAAGGGTACGATACCAGGAAACAGACCAAATGGG
TGTGGTTTATTATGCCAATT

Product: pyruvate kinase

Products: NA

Alternate protein names: PK; Vegetative protein 17; VEG17 [H]

Number of amino acids: Translated: 580; Mature: 580

Protein sequence:

>580_residues
MLRKTKIVATIGPACENKESLARMAQNGMDVCRLNFSHGEHQEHLDRINLIREVYQELKFPPAILLDTRGPEIRLGTFEN
GEVDLEKGQEFTLSTEEIEGDTTRVHVNYPQVTSDISPGDKVLLDDGLIELLVNEVTETEVKTVVNNDGTIKDNKGVNLP
GVTLSLPAMTDKDQQDILFGIENQVDFIAASFIRKPEDVLEIRKFLEEHGSDIPVISKIENKEGVENLERILEVSDGLMV
ARGDLGVDIPPEEVPLVQKRMINRCNDEGKPVITATQMLESMTENPRPTRAEASDVANAIIDGTDAVMLSGETAAGAYPV
ESVSTMANIAKSTERELSRKPQEVTAKDTGMTEAIGYSTCQIAGQLEANAILTATQSGYTARMVAKYKPKAPIVAVTPIE
KVARRLSLSWGVYPITCDPSETTDEMFQEAIQTALITQYISEGDLIVITAGIPVGVSGTTNLIRVHTVGEILVSGAGIGK
KPATGKVVKVEDSNDLSKISSGDIVVLRGTTDEMVPHLAQVSGLVAEEGGYTSPSAIVGLDLGLPVIVGAEGALEKLADG
DQVTIDPLRGFVYKGQATIL

Sequences:

>Translated_580_residues
MLRKTKIVATIGPACENKESLARMAQNGMDVCRLNFSHGEHQEHLDRINLIREVYQELKFPPAILLDTRGPEIRLGTFEN
GEVDLEKGQEFTLSTEEIEGDTTRVHVNYPQVTSDISPGDKVLLDDGLIELLVNEVTETEVKTVVNNDGTIKDNKGVNLP
GVTLSLPAMTDKDQQDILFGIENQVDFIAASFIRKPEDVLEIRKFLEEHGSDIPVISKIENKEGVENLERILEVSDGLMV
ARGDLGVDIPPEEVPLVQKRMINRCNDEGKPVITATQMLESMTENPRPTRAEASDVANAIIDGTDAVMLSGETAAGAYPV
ESVSTMANIAKSTERELSRKPQEVTAKDTGMTEAIGYSTCQIAGQLEANAILTATQSGYTARMVAKYKPKAPIVAVTPIE
KVARRLSLSWGVYPITCDPSETTDEMFQEAIQTALITQYISEGDLIVITAGIPVGVSGTTNLIRVHTVGEILVSGAGIGK
KPATGKVVKVEDSNDLSKISSGDIVVLRGTTDEMVPHLAQVSGLVAEEGGYTSPSAIVGLDLGLPVIVGAEGALEKLADG
DQVTIDPLRGFVYKGQATIL
>Mature_580_residues
MLRKTKIVATIGPACENKESLARMAQNGMDVCRLNFSHGEHQEHLDRINLIREVYQELKFPPAILLDTRGPEIRLGTFEN
GEVDLEKGQEFTLSTEEIEGDTTRVHVNYPQVTSDISPGDKVLLDDGLIELLVNEVTETEVKTVVNNDGTIKDNKGVNLP
GVTLSLPAMTDKDQQDILFGIENQVDFIAASFIRKPEDVLEIRKFLEEHGSDIPVISKIENKEGVENLERILEVSDGLMV
ARGDLGVDIPPEEVPLVQKRMINRCNDEGKPVITATQMLESMTENPRPTRAEASDVANAIIDGTDAVMLSGETAAGAYPV
ESVSTMANIAKSTERELSRKPQEVTAKDTGMTEAIGYSTCQIAGQLEANAILTATQSGYTARMVAKYKPKAPIVAVTPIE
KVARRLSLSWGVYPITCDPSETTDEMFQEAIQTALITQYISEGDLIVITAGIPVGVSGTTNLIRVHTVGEILVSGAGIGK
KPATGKVVKVEDSNDLSKISSGDIVVLRGTTDEMVPHLAQVSGLVAEEGGYTSPSAIVGLDLGLPVIVGAEGALEKLADG
DQVTIDPLRGFVYKGQATIL

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the PEP- utilizing enzyme family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=486, Percent_Identity=44.238683127572, Blast_Score=387, Evalue=1e-107,
Organism=Homo sapiens, GI33286420, Length=486, Percent_Identity=44.238683127572, Blast_Score=387, Evalue=1e-107,
Organism=Homo sapiens, GI10835121, Length=499, Percent_Identity=42.685370741483, Blast_Score=383, Evalue=1e-106,
Organism=Homo sapiens, GI32967597, Length=499, Percent_Identity=42.685370741483, Blast_Score=383, Evalue=1e-106,
Organism=Homo sapiens, GI33286418, Length=486, Percent_Identity=44.0329218106996, Blast_Score=380, Evalue=1e-105,
Organism=Homo sapiens, GI310128732, Length=287, Percent_Identity=47.3867595818815, Blast_Score=245, Evalue=1e-64,
Organism=Homo sapiens, GI310128730, Length=287, Percent_Identity=47.3867595818815, Blast_Score=245, Evalue=1e-64,
Organism=Homo sapiens, GI310128736, Length=235, Percent_Identity=43.8297872340426, Blast_Score=181, Evalue=1e-45,
Organism=Homo sapiens, GI310128734, Length=235, Percent_Identity=43.8297872340426, Blast_Score=181, Evalue=1e-45,
Organism=Homo sapiens, GI310128738, Length=214, Percent_Identity=43.4579439252336, Blast_Score=163, Evalue=4e-40,
Organism=Escherichia coli, GI1787965, Length=471, Percent_Identity=50.7430997876858, Blast_Score=434, Evalue=1e-123,
Organism=Escherichia coli, GI1788160, Length=475, Percent_Identity=39.7894736842105, Blast_Score=317, Evalue=1e-87,
Organism=Escherichia coli, GI1787994, Length=112, Percent_Identity=32.1428571428571, Blast_Score=70, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17544584, Length=480, Percent_Identity=44.375, Blast_Score=400, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI17506829, Length=486, Percent_Identity=43.4156378600823, Blast_Score=351, Evalue=5e-97,
Organism=Caenorhabditis elegans, GI71984413, Length=486, Percent_Identity=43.4156378600823, Blast_Score=351, Evalue=6e-97,
Organism=Caenorhabditis elegans, GI71984406, Length=486, Percent_Identity=43.4156378600823, Blast_Score=351, Evalue=7e-97,
Organism=Caenorhabditis elegans, GI17506831, Length=486, Percent_Identity=43.4156378600823, Blast_Score=349, Evalue=2e-96,
Organism=Saccharomyces cerevisiae, GI6319279, Length=486, Percent_Identity=43.2098765432099, Blast_Score=354, Evalue=2e-98,
Organism=Saccharomyces cerevisiae, GI6324923, Length=486, Percent_Identity=40.3292181069959, Blast_Score=340, Evalue=3e-94,
Organism=Drosophila melanogaster, GI24648964, Length=494, Percent_Identity=44.331983805668, Blast_Score=384, Evalue=1e-106,
Organism=Drosophila melanogaster, GI28571814, Length=494, Percent_Identity=44.331983805668, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24648966, Length=408, Percent_Identity=46.5686274509804, Blast_Score=329, Evalue=3e-90,
Organism=Drosophila melanogaster, GI24581235, Length=483, Percent_Identity=36.231884057971, Blast_Score=288, Evalue=8e-78,
Organism=Drosophila melanogaster, GI24646914, Length=276, Percent_Identity=42.0289855072464, Blast_Score=195, Evalue=9e-50,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 62483; Mature: 62483

Theoretical pI: Translated: 4.36; Mature: 4.36

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRKTKIVATIGPACENKESLARMAQNGMDVCRLNFSHGEHQEHLDRINLIREVYQELKF
CCCCEEEEEEECCCCCCHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCC
PPAILLDTRGPEIRLGTFENGEVDLEKGQEFTLSTEEIEGDTTRVHVNYPQVTSDISPGD
CCEEEEECCCCEEEEECCCCCCEECCCCCEEEEEHHHCCCCEEEEEEECCCCCCCCCCCC
KVLLDDGLIELLVNEVTETEVKTVVNNDGTIKDNKGVNLPGVTLSLPAMTDKDQQDILFG
EEEECCHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCEEECCCCCCCCCCCEEEE
IENQVDFIAASFIRKPEDVLEIRKFLEEHGSDIPVISKIENKEGVENLERILEVSDGLMV
CCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHHCCCEEE
ARGDLGVDIPPEEVPLVQKRMINRCNDEGKPVITATQMLESMTENPRPTRAEASDVANAI
EECCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEHHHHHHHHHHCCCCCCCCCHHHHHHHH
IDGTDAVMLSGETAAGAYPVESVSTMANIAKSTERELSRKPQEVTAKDTGMTEAIGYSTC
HCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCEE
QIAGQLEANAILTATQSGYTARMVAKYKPKAPIVAVTPIEKVARRLSLSWGVYPITCDPS
EEECCCCCCEEEEEECCCCEEEEEEEECCCCCEEEECCHHHHHHHHHCCCCEEEEECCCC
ETTDEMFQEAIQTALITQYISEGDLIVITAGIPVGVSGTTNLIRVHTVGEILVSGAGIGK
CCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCEEEEEEHHHHHHCCCCCCC
KPATGKVVKVEDSNDLSKISSGDIVVLRGTTDEMVPHLAQVSGLVAEEGGYTSPSAIVGL
CCCCCCEEEEECCCCHHHCCCCCEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEE
DLGLPVIVGAEGALEKLADGDQVTIDPLRGFVYKGQATIL
CCCCCEEECCCHHHHHHCCCCEEEEECCCCEEECCCEECC
>Mature Secondary Structure
MLRKTKIVATIGPACENKESLARMAQNGMDVCRLNFSHGEHQEHLDRINLIREVYQELKF
CCCCEEEEEEECCCCCCHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCC
PPAILLDTRGPEIRLGTFENGEVDLEKGQEFTLSTEEIEGDTTRVHVNYPQVTSDISPGD
CCEEEEECCCCEEEEECCCCCCEECCCCCEEEEEHHHCCCCEEEEEEECCCCCCCCCCCC
KVLLDDGLIELLVNEVTETEVKTVVNNDGTIKDNKGVNLPGVTLSLPAMTDKDQQDILFG
EEEECCHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCEEECCCCCCCCCCCEEEE
IENQVDFIAASFIRKPEDVLEIRKFLEEHGSDIPVISKIENKEGVENLERILEVSDGLMV
CCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHHCCCEEE
ARGDLGVDIPPEEVPLVQKRMINRCNDEGKPVITATQMLESMTENPRPTRAEASDVANAI
EECCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEHHHHHHHHHHCCCCCCCCCHHHHHHHH
IDGTDAVMLSGETAAGAYPVESVSTMANIAKSTERELSRKPQEVTAKDTGMTEAIGYSTC
HCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCEE
QIAGQLEANAILTATQSGYTARMVAKYKPKAPIVAVTPIEKVARRLSLSWGVYPITCDPS
EEECCCCCCEEEEEECCCCEEEEEEEECCCCCEEEECCHHHHHHHHHCCCCEEEEECCCC
ETTDEMFQEAIQTALITQYISEGDLIVITAGIPVGVSGTTNLIRVHTVGEILVSGAGIGK
CCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCEEEEEEHHHHHHCCCCCCC
KPATGKVVKVEDSNDLSKISSGDIVVLRGTTDEMVPHLAQVSGLVAEEGGYTSPSAIVGL
CCCCCCEEEEECCCCHHHCCCCCEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEE
DLGLPVIVGAEGALEKLADGDQVTIDPLRGFVYKGQATIL
CCCCCEEECCCHHHHHHCCCCEEEEECCCCEEECCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 9298659 [H]