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The map label for this gene is purK [H]

Identifier: 188585092

GI number: 188585092

Start: 478122

End: 479285

Strand: Direct

Name: purK [H]

Synonym: Nther_0453

Alternate gene names: 188585092

Gene position: 478122-479285 (Clockwise)

Preceding gene: 188585091

Following gene: 188585093

Centisome position: 15.1

GC content: 37.63

Gene sequence:

>1164_bases
ATGTACTCCCTGTATTCACCTGAACTAACTTTGGGAATAGTTGGTGGCGGACAACTTGGTAGAATGATGACCTTTGAAGC
AAAAAAACTTGGCTTAAAAGTTGTGGTTTTGGACCCAACAGAACAATCTCCAGCTGCCCAAGTTGCTGATGAACAGATTG
TCTCATCCTTTGATGATCTAGATGGTTATCGTACTCTGGCCAAACTTAGTGATATTATCACATACGAATTTGAACATATC
CATGTGTCTGGATTACAAGAAATTGAAGCAGCAGGGGTGCCAGTTAGACCAGGAAGCGCTACTTTAGCTTCAATTCAACA
TAAAGGAAAACAAAAAGAGCTGTTAAGAACACACGGGATAGCTGTGCCGTCTTTTCAGTATGCCAAAGGATATCAACAAG
CTGCCTCAGAACTTAATAAATTCAAATTTCCAGTGATGGCTAAATTATGCCATGGTGGATACGATGGAAAAGGTAATATC
TTACTTGAAAATGAATCTGATTTACGGAAATTAGAGACTATGGCAGGAGAAAATCGGGAATTAATGTTGGAAAAATACTT
AAGATTTGATAAAGAACTGTCTGTTATGGCTGCCAAAAGCGCCAATGGTGATACAGCAGTTTTTCCTGTAGTAGAAAATG
TTCACAAAGATGAGATATTACATTTAACCAAAGCTCCAGCTGATATCAGTTCATATGCAAGAGATCAATTAATAAGTACT
GCCGAAAAAATCCTTGAAATATTTTCAGAACCAGGTATCTATGGAATAGAGTTTTTTCTTCTCGAAGATGACAGCATCAT
GGTAAATGAAATCGCACCCAGGGTACATAACTCGGGCCATTACACTATAGAAGCTTGTAATTTATGTCAATTTGCTGTTC
ATTTAAGAAGTATTCTCGGTCTACCTTTAATTCATCCTAAACTAGAAAAGAACGCTGTGATGATCAATTTACTGGGAAGT
GAGCAGGGAGAACTACCCATTACCAATTATGATTCACTTCTGACCCACGAAGGTGTTTACTTACATCTGTACGGTAAACA
AGAAACTCGTCCTGGAAGGAAAATGGGTCATATCACAATTACTGGACAAAATGAACAGGACCTTGTATCTAAAGTCAATA
ACTTGGAAATTATTGATAAAAGGGGGATCTTGGATGAATTATAA

Upstream 100 bases:

>100_bases
ATTAAGGGTACACCGAAGGGATAAAAGCCCAGGCGGGTAGGTTTCTCTCGATTCCTACTTGCCTGGGTTTTTTTGAAATA
TTTTAATGGAGGGTTGTTGT

Downstream 100 bases:

>100_bases
AGTTGGAGTGATAATGGGAAGTGATTCTGATCTACCTGTTTTACAAGATGCCTGTGATGTTTTGGAAGAATTTCAAATTC
CCTACGAGGTAGATATAGTT

Product: phosphoribosylaminoimidazole carboxylase, ATPase subunit

Products: NA

Alternate protein names: N5-CAIR synthase; 5-(carboxyamino)imidazole ribonucleotide synthetase [H]

Number of amino acids: Translated: 387; Mature: 387

Protein sequence:

>387_residues
MYSLYSPELTLGIVGGGQLGRMMTFEAKKLGLKVVVLDPTEQSPAAQVADEQIVSSFDDLDGYRTLAKLSDIITYEFEHI
HVSGLQEIEAAGVPVRPGSATLASIQHKGKQKELLRTHGIAVPSFQYAKGYQQAASELNKFKFPVMAKLCHGGYDGKGNI
LLENESDLRKLETMAGENRELMLEKYLRFDKELSVMAAKSANGDTAVFPVVENVHKDEILHLTKAPADISSYARDQLIST
AEKILEIFSEPGIYGIEFFLLEDDSIMVNEIAPRVHNSGHYTIEACNLCQFAVHLRSILGLPLIHPKLEKNAVMINLLGS
EQGELPITNYDSLLTHEGVYLHLYGKQETRPGRKMGHITITGQNEQDLVSKVNNLEIIDKRGILDEL

Sequences:

>Translated_387_residues
MYSLYSPELTLGIVGGGQLGRMMTFEAKKLGLKVVVLDPTEQSPAAQVADEQIVSSFDDLDGYRTLAKLSDIITYEFEHI
HVSGLQEIEAAGVPVRPGSATLASIQHKGKQKELLRTHGIAVPSFQYAKGYQQAASELNKFKFPVMAKLCHGGYDGKGNI
LLENESDLRKLETMAGENRELMLEKYLRFDKELSVMAAKSANGDTAVFPVVENVHKDEILHLTKAPADISSYARDQLIST
AEKILEIFSEPGIYGIEFFLLEDDSIMVNEIAPRVHNSGHYTIEACNLCQFAVHLRSILGLPLIHPKLEKNAVMINLLGS
EQGELPITNYDSLLTHEGVYLHLYGKQETRPGRKMGHITITGQNEQDLVSKVNNLEIIDKRGILDEL
>Mature_387_residues
MYSLYSPELTLGIVGGGQLGRMMTFEAKKLGLKVVVLDPTEQSPAAQVADEQIVSSFDDLDGYRTLAKLSDIITYEFEHI
HVSGLQEIEAAGVPVRPGSATLASIQHKGKQKELLRTHGIAVPSFQYAKGYQQAASELNKFKFPVMAKLCHGGYDGKGNI
LLENESDLRKLETMAGENRELMLEKYLRFDKELSVMAAKSANGDTAVFPVVENVHKDEILHLTKAPADISSYARDQLIST
AEKILEIFSEPGIYGIEFFLLEDDSIMVNEIAPRVHNSGHYTIEACNLCQFAVHLRSILGLPLIHPKLEKNAVMINLLGS
EQGELPITNYDSLLTHEGVYLHLYGKQETRPGRKMGHITITGQNEQDLVSKVNNLEIIDKRGILDEL

Specific function: Catalyzes the ATP-dependent conversion of 5- aminoimidazole ribonucleotide (AIR) and HCO(3)(-) to N5- carboxyaminoimidazole ribonucleotide (N5-CAIR) [H]

COG id: COG0026

COG function: function code F; Phosphoribosylaminoimidazole carboxylase (NCAIR synthetase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786733, Length=364, Percent_Identity=29.6703296703297, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI1788155, Length=376, Percent_Identity=23.4042553191489, Blast_Score=101, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6324702, Length=381, Percent_Identity=37.7952755905512, Blast_Score=227, Evalue=2e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006115
- InterPro:   IPR005875
- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR011054 [H]

Pfam domain/function: PF02222 ATP-grasp; PF03446 NAD_binding_2 [H]

EC number: =6.3.4.18 [H]

Molecular weight: Translated: 42904; Mature: 42904

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYSLYSPELTLGIVGGGQLGRMMTFEAKKLGLKVVVLDPTEQSPAAQVADEQIVSSFDDL
CCCCCCCCEEEEEECCCCCCCEEEEEHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHHHH
DGYRTLAKLSDIITYEFEHIHVSGLQEIEAAGVPVRPGSATLASIQHKGKQKELLRTHGI
HHHHHHHHHHHHHEEEEEEEEECCHHHHHHCCCCCCCCCHHHHHHHHCCHHHHHHHHCCC
AVPSFQYAKGYQQAASELNKFKFPVMAKLCHGGYDGKGNILLENESDLRKLETMAGENRE
CCCCHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCEEEECCHHHHHHHHHCCCCHH
LMLEKYLRFDKELSVMAAKSANGDTAVFPVVENVHKDEILHLTKAPADISSYARDQLIST
HHHHHHHCCCCHHEEEEECCCCCCEEEEHHHHHCCHHHEEEEECCCCHHHHHHHHHHHHH
AEKILEIFSEPGIYGIEFFLLEDDSIMVNEIAPRVHNSGHYTIEACNLCQFAVHLRSILG
HHHHHHHHHCCCCEEEEEEEEECCCEEEHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHC
LPLIHPKLEKNAVMINLLGSEQGELPITNYDSLLTHEGVYLHLYGKQETRPGRKMGHITI
CCCCCCCCCCCEEEEEEECCCCCCCCCCCCHHHHCCCCEEEEEECCCCCCCCCEEEEEEE
TGQNEQDLVSKVNNLEIIDKRGILDEL
ECCCHHHHHHHCCCCEEEECCCCCCCC
>Mature Secondary Structure
MYSLYSPELTLGIVGGGQLGRMMTFEAKKLGLKVVVLDPTEQSPAAQVADEQIVSSFDDL
CCCCCCCCEEEEEECCCCCCCEEEEEHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHHHH
DGYRTLAKLSDIITYEFEHIHVSGLQEIEAAGVPVRPGSATLASIQHKGKQKELLRTHGI
HHHHHHHHHHHHHEEEEEEEEECCHHHHHHCCCCCCCCCHHHHHHHHCCHHHHHHHHCCC
AVPSFQYAKGYQQAASELNKFKFPVMAKLCHGGYDGKGNILLENESDLRKLETMAGENRE
CCCCHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCEEEECCHHHHHHHHHCCCCHH
LMLEKYLRFDKELSVMAAKSANGDTAVFPVVENVHKDEILHLTKAPADISSYARDQLIST
HHHHHHHCCCCHHEEEEECCCCCCEEEEHHHHHCCHHHEEEEECCCCHHHHHHHHHHHHH
AEKILEIFSEPGIYGIEFFLLEDDSIMVNEIAPRVHNSGHYTIEACNLCQFAVHLRSILG
HHHHHHHHHCCCCEEEEEEEEECCCEEEHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHC
LPLIHPKLEKNAVMINLLGSEQGELPITNYDSLLTHEGVYLHLYGKQETRPGRKMGHITI
CCCCCCCCCCCEEEEEEECCCCCCCCCCCCHHHHCCCCEEEEEECCCCCCCCCEEEEEEE
TGQNEQDLVSKVNNLEIIDKRGILDEL
ECCCHHHHHHHCCCCEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377 [H]