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The map label for this gene is groL

Identifier: 188585089

GI number: 188585089

Start: 473698

End: 475344

Strand: Direct

Name: groL

Synonym: Nther_0450

Alternate gene names: 188585089

Gene position: 473698-475344 (Clockwise)

Preceding gene: 188585088

Following gene: 188585090

Centisome position: 14.96

GC content: 40.62

Gene sequence:

>1647_bases
ATGGCAAAGGACATTAAATTTAGAGAAGATGCTCGTGCCAGATTAGAGCAAGGTGTAAATAAGTTAGCTGACACCTTAAA
AGTAACTTTGGGACCTAAAGGTCGCAATGTTGTTCTTGATAAAAAGTTTGGCTCACCTCAAATAACAAATGACGGTGTAA
CTATTGCTAGAGATATTGATTTAGAAGATAATTATGAAAATATGGGAGCTCAGTTGGTTAAAGAGGTTGCAACTCAAACC
AATGACGTAGCTGGAGACGGTACAACTACTGCAACTATTCTAGCACAGGCTATGGTTAATGAAGGAATTAAGAACGTAAC
TGCTGGTGCCAACCCCATGATAATCAGAAAAGGAATCCAAAAAGCTGTCGATAGAGCAGTTGAAGAGTTGCAGAAAAACG
CTGTCAGTGTTGAAGATAAAGAGTCCATTTCTCAAGTTGCTTCAATCTCAGCTAATGACGAAGAAGTAGGTAAGCTAATT
GCTGAAGCTATGGAAAAAGTAGGCAAAGATGGAGTAATCACAGTAGAAGAATCCAAGAGCTTCAAAACTGATTTAAACGT
AGTTGAAGGTATGCAGTTTGATAGAGGATATGTTTCACCTTATATGGTAACTGATAATGAAAAGATGGAAGCTCACTTAG
AAGAGCCATACATCTTGATTACCGATAAGAAGATCGGTAATATCCAAGAGATTTTACCAGTACTAGAAAAAATCGTTGAA
CAAGGTAAAGAAGTTCTGTTAATCGCAGAAGACATCGAAGGCGAAGCCTTGGCTACATTAGTTGTTAACAAGTTGAGAGG
AACCTTCACTTGTGTGGGAGTTAAAGCTCCTGGTTTTGGTGACAGAAGAAAAGCTATGCTTGAAGATATTGCCGTACTTA
CAGGTGGTCAGGTAATCAGTGAAGATGTAGGACTTGAACTTAAGAATGCTGATATCAGCATGTTAGGTCGAGCTAGACAG
GTCACCATCACTAAAGACGATACAACTATCGTAGATGGCTATGGAAATGAAGAAGATATCCAAAAACGAATTACTCAATT
AAGAACTCAAATTGAAGAAACTACCTCAGATTTCGATAGAGAAAAACTAGAAGAGCGCTTAGCTAAACTAGCTGGAGGAG
TTGCAGTAGTTGAAGTTGGAGCAGCTACTGAAACTGAAATGAAAGAAAAGAAACTACGAATTGAAGACGCACTTAACTCT
ACTAGAGCTGCTGTGGAAGAAGGAATCGTAGCTGGTGGTGGAACAGCCCTAATTGATGTATTACCTTCCCTTGAAGAAGT
TCAAGCGGATGGTGACGAGTCTACAGGAGTTAGCATCGTTAGACGTGCTCTAGAAGAACCTGTACGTCAATTGGCACATA
ACTCTGGTGCTGAGGGCTCCATAGTTGCTGAACAAGTTAAGCAAAAAGGAACAAACATAGGATTTAATGCCCTTGAAAAC
GATTACACTAATATGCTCGATGCTGGTGTAGTTGATCCTAAGAAAGTTACTAGAAGCGCCCTTGAAAATGCAGGAAGTAT
CGCTGCAATGTTCTTAACAACTGAAGCAGTAGTAGCAGATCTACCAGATGAGGACGATAACGATGATGGAGACATGGGCG
GTGGCGCCCCAGGAATGGGAGGCATGGGCGGAATGCCCGGAATGTAA

Upstream 100 bases:

>100_bases
GAAATGAAGTTGAGGTTGATGGTGAAGAATACTTAATCATGCGCCAGGATGATATCTTGGCGGTTATAGAATAAAACCCA
CATAAAGGAGGTAATGTGCT

Downstream 100 bases:

>100_bases
GCCCATTCCCCTAATAGGTAATTAGAAATTAGAGACCCCGGTCCCCTTTAAAGGACCGGGGTCTTTTGTTTGACATTGCA
TTTAGCAAGCTATATAATTT

Product: chaperonin GroEL

Products: NA

Alternate protein names: GroEL protein; Protein Cpn60

Number of amino acids: Translated: 548; Mature: 547

Protein sequence:

>548_residues
MAKDIKFREDARARLEQGVNKLADTLKVTLGPKGRNVVLDKKFGSPQITNDGVTIARDIDLEDNYENMGAQLVKEVATQT
NDVAGDGTTTATILAQAMVNEGIKNVTAGANPMIIRKGIQKAVDRAVEELQKNAVSVEDKESISQVASISANDEEVGKLI
AEAMEKVGKDGVITVEESKSFKTDLNVVEGMQFDRGYVSPYMVTDNEKMEAHLEEPYILITDKKIGNIQEILPVLEKIVE
QGKEVLLIAEDIEGEALATLVVNKLRGTFTCVGVKAPGFGDRRKAMLEDIAVLTGGQVISEDVGLELKNADISMLGRARQ
VTITKDDTTIVDGYGNEEDIQKRITQLRTQIEETTSDFDREKLEERLAKLAGGVAVVEVGAATETEMKEKKLRIEDALNS
TRAAVEEGIVAGGGTALIDVLPSLEEVQADGDESTGVSIVRRALEEPVRQLAHNSGAEGSIVAEQVKQKGTNIGFNALEN
DYTNMLDAGVVDPKKVTRSALENAGSIAAMFLTTEAVVADLPDEDDNDDGDMGGGAPGMGGMGGMPGM

Sequences:

>Translated_548_residues
MAKDIKFREDARARLEQGVNKLADTLKVTLGPKGRNVVLDKKFGSPQITNDGVTIARDIDLEDNYENMGAQLVKEVATQT
NDVAGDGTTTATILAQAMVNEGIKNVTAGANPMIIRKGIQKAVDRAVEELQKNAVSVEDKESISQVASISANDEEVGKLI
AEAMEKVGKDGVITVEESKSFKTDLNVVEGMQFDRGYVSPYMVTDNEKMEAHLEEPYILITDKKIGNIQEILPVLEKIVE
QGKEVLLIAEDIEGEALATLVVNKLRGTFTCVGVKAPGFGDRRKAMLEDIAVLTGGQVISEDVGLELKNADISMLGRARQ
VTITKDDTTIVDGYGNEEDIQKRITQLRTQIEETTSDFDREKLEERLAKLAGGVAVVEVGAATETEMKEKKLRIEDALNS
TRAAVEEGIVAGGGTALIDVLPSLEEVQADGDESTGVSIVRRALEEPVRQLAHNSGAEGSIVAEQVKQKGTNIGFNALEN
DYTNMLDAGVVDPKKVTRSALENAGSIAAMFLTTEAVVADLPDEDDNDDGDMGGGAPGMGGMGGMPGM
>Mature_547_residues
AKDIKFREDARARLEQGVNKLADTLKVTLGPKGRNVVLDKKFGSPQITNDGVTIARDIDLEDNYENMGAQLVKEVATQTN
DVAGDGTTTATILAQAMVNEGIKNVTAGANPMIIRKGIQKAVDRAVEELQKNAVSVEDKESISQVASISANDEEVGKLIA
EAMEKVGKDGVITVEESKSFKTDLNVVEGMQFDRGYVSPYMVTDNEKMEAHLEEPYILITDKKIGNIQEILPVLEKIVEQ
GKEVLLIAEDIEGEALATLVVNKLRGTFTCVGVKAPGFGDRRKAMLEDIAVLTGGQVISEDVGLELKNADISMLGRARQV
TITKDDTTIVDGYGNEEDIQKRITQLRTQIEETTSDFDREKLEERLAKLAGGVAVVEVGAATETEMKEKKLRIEDALNST
RAAVEEGIVAGGGTALIDVLPSLEEVQADGDESTGVSIVRRALEEPVRQLAHNSGAEGSIVAEQVKQKGTNIGFNALEND
YTNMLDAGVVDPKKVTRSALENAGSIAAMFLTTEAVVADLPDEDDNDDGDMGGGAPGMGGMGGMPGM

Specific function: Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions

COG id: COG0459

COG function: function code O; Chaperonin GroEL (HSP60 family)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the chaperonin (HSP60) family

Homologues:

Organism=Homo sapiens, GI41399285, Length=530, Percent_Identity=47.3584905660377, Blast_Score=513, Evalue=1e-145,
Organism=Homo sapiens, GI31542947, Length=530, Percent_Identity=47.3584905660377, Blast_Score=513, Evalue=1e-145,
Organism=Homo sapiens, GI4502643, Length=234, Percent_Identity=26.4957264957265, Blast_Score=70, Evalue=5e-12,
Organism=Homo sapiens, GI58331173, Length=216, Percent_Identity=25.9259259259259, Blast_Score=68, Evalue=2e-11,
Organism=Homo sapiens, GI38455427, Length=139, Percent_Identity=30.2158273381295, Blast_Score=67, Evalue=4e-11,
Organism=Escherichia coli, GI1790586, Length=529, Percent_Identity=61.0586011342155, Blast_Score=644, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17555558, Length=530, Percent_Identity=46.6037735849057, Blast_Score=482, Evalue=1e-136,
Organism=Caenorhabditis elegans, GI193210679, Length=210, Percent_Identity=46.1904761904762, Blast_Score=193, Evalue=3e-49,
Organism=Caenorhabditis elegans, GI25144674, Length=177, Percent_Identity=31.638418079096, Blast_Score=69, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6323288, Length=524, Percent_Identity=49.8091603053435, Blast_Score=512, Evalue=1e-146,
Organism=Saccharomyces cerevisiae, GI6320058, Length=167, Percent_Identity=29.3413173652695, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6322446, Length=228, Percent_Identity=26.7543859649123, Blast_Score=64, Evalue=7e-11,
Organism=Drosophila melanogaster, GI24641193, Length=527, Percent_Identity=48.1973434535104, Blast_Score=514, Evalue=1e-146,
Organism=Drosophila melanogaster, GI24641191, Length=527, Percent_Identity=48.1973434535104, Blast_Score=514, Evalue=1e-146,
Organism=Drosophila melanogaster, GI45550936, Length=527, Percent_Identity=45.9203036053131, Blast_Score=483, Evalue=1e-136,
Organism=Drosophila melanogaster, GI45550132, Length=527, Percent_Identity=45.9203036053131, Blast_Score=483, Evalue=1e-136,
Organism=Drosophila melanogaster, GI45550935, Length=527, Percent_Identity=45.9203036053131, Blast_Score=483, Evalue=1e-136,
Organism=Drosophila melanogaster, GI17864606, Length=528, Percent_Identity=41.0984848484849, Blast_Score=422, Evalue=1e-118,
Organism=Drosophila melanogaster, GI24584129, Length=529, Percent_Identity=34.4045368620038, Blast_Score=296, Evalue=2e-80,
Organism=Drosophila melanogaster, GI19921262, Length=529, Percent_Identity=34.4045368620038, Blast_Score=296, Evalue=2e-80,
Organism=Drosophila melanogaster, GI24583944, Length=175, Percent_Identity=28, Blast_Score=69, Evalue=1e-11,
Organism=Drosophila melanogaster, GI17647245, Length=518, Percent_Identity=23.7451737451737, Blast_Score=67, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24652903, Length=518, Percent_Identity=23.7451737451737, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: 2180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 480 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 15012 Molecules/Cell In: Growth Phase,

Swissprot (AC and ID): CH60_NATTJ (B2A5V3)

Other databases:

- EMBL:   CP001034
- RefSeq:   YP_001916634.1
- ProteinModelPortal:   B2A5V3
- SMR:   B2A5V3
- GeneID:   6315275
- GenomeReviews:   CP001034_GR
- KEGG:   nth:Nther_0450
- HOGENOM:   HBG625289
- OMA:   SANDEEV
- GO:   GO:0005737
- HAMAP:   MF_00600
- InterPro:   IPR018370
- InterPro:   IPR001844
- InterPro:   IPR002423
- PANTHER:   PTHR11353
- PRINTS:   PR00298
- TIGRFAMs:   TIGR02348

Pfam domain/function: PF00118 Cpn60_TCP1; SSF48592 GroEL-ATPase

EC number: NA

Molecular weight: Translated: 58679; Mature: 58548

Theoretical pI: Translated: 4.26; Mature: 4.26

Prosite motif: PS00296 CHAPERONINS_CPN60

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKDIKFREDARARLEQGVNKLADTLKVTLGPKGRNVVLDKKFGSPQITNDGVTIARDID
CCCCCCCHHHHHHHHHHHHHHHHHHHEEEECCCCCEEEEECCCCCCCCCCCCEEEEEECC
LEDNYENMGAQLVKEVATQTNDVAGDGTTTATILAQAMVNEGIKNVTAGANPMIIRKGIQ
CCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
KAVDRAVEELQKNAVSVEDKESISQVASISANDEEVGKLIAEAMEKVGKDGVITVEESKS
HHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCC
FKTDLNVVEGMQFDRGYVSPYMVTDNEKMEAHLEEPYILITDKKIGNIQEILPVLEKIVE
HHHHHHHHHCCCCCCCCCCCEEEECCHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHH
QGKEVLLIAEDIEGEALATLVVNKLRGTFTCVGVKAPGFGDRRKAMLEDIAVLTGGQVIS
CCCEEEEEEECCCCHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCHHHH
EDVGLELKNADISMLGRARQVTITKDDTTIVDGYGNEEDIQKRITQLRTQIEETTSDFDR
HCCCCEECCCCHHHHCCCEEEEEECCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
EKLEERLAKLAGGVAVVEVGAATETEMKEKKLRIEDALNSTRAAVEEGIVAGGGTALIDV
HHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
LPSLEEVQADGDESTGVSIVRRALEEPVRQLAHNSGAEGSIVAEQVKQKGTNIGFNALEN
HCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCHHHHH
DYTNMLDAGVVDPKKVTRSALENAGSIAAMFLTTEAVVADLPDEDDNDDGDMGGGAPGMG
HHHHHHHCCCCCHHHHHHHHHHCCCCEEEHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
GMGGMPGM
CCCCCCCC
>Mature Secondary Structure 
AKDIKFREDARARLEQGVNKLADTLKVTLGPKGRNVVLDKKFGSPQITNDGVTIARDID
CCCCCCHHHHHHHHHHHHHHHHHHHEEEECCCCCEEEEECCCCCCCCCCCCEEEEEECC
LEDNYENMGAQLVKEVATQTNDVAGDGTTTATILAQAMVNEGIKNVTAGANPMIIRKGIQ
CCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
KAVDRAVEELQKNAVSVEDKESISQVASISANDEEVGKLIAEAMEKVGKDGVITVEESKS
HHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCC
FKTDLNVVEGMQFDRGYVSPYMVTDNEKMEAHLEEPYILITDKKIGNIQEILPVLEKIVE
HHHHHHHHHCCCCCCCCCCCEEEECCHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHH
QGKEVLLIAEDIEGEALATLVVNKLRGTFTCVGVKAPGFGDRRKAMLEDIAVLTGGQVIS
CCCEEEEEEECCCCHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCHHHH
EDVGLELKNADISMLGRARQVTITKDDTTIVDGYGNEEDIQKRITQLRTQIEETTSDFDR
HCCCCEECCCCHHHHCCCEEEEEECCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
EKLEERLAKLAGGVAVVEVGAATETEMKEKKLRIEDALNSTRAAVEEGIVAGGGTALIDV
HHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
LPSLEEVQADGDESTGVSIVRRALEEPVRQLAHNSGAEGSIVAEQVKQKGTNIGFNALEN
HCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCHHHHH
DYTNMLDAGVVDPKKVTRSALENAGSIAAMFLTTEAVVADLPDEDDNDDGDMGGGAPGMG
HHHHHHHCCCCCHHHHHHHHHHCCCCEEEHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
GMGGMPGM
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA