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The map label for this gene is proS

Identifier: 188585074

GI number: 188585074

Start: 456275

End: 457726

Strand: Direct

Name: proS

Synonym: Nther_0435

Alternate gene names: 188585074

Gene position: 456275-457726 (Clockwise)

Preceding gene: 188585072

Following gene: 188585075

Centisome position: 14.41

GC content: 38.36

Gene sequence:

>1452_bases
ATGTCTAAAAACAATGAGGAATTCGTTAAAGAAATAACCCCTCAATCTGAAGACTATTCCCAGTGGTATCTGGATGTGAT
TAAAAAGACTAAACTTGTGGATTACGCTCCTGTAAAAGGTTGTATGGTGATTAGACCCTATGGTTATGCAATTTGGGAAA
AAATGAAGGAAGGCCTAGATCGTAGAATCAAAGAGACGGGTCACGAAAATGCTTATTTTCCGTTATTTATTCCAGAATCT
TTACTACAAAAGGAAGCAGATCACGTAGAAGGGTTTGCTCCTGAAGTTGCCTGGATTACTAAAGGTGGAGATGAAGAACT
ATCAGAGTCTTTAGCTGTCAGGCCTACTTCGGAAGCTATGTTTGGTGAAATGTACAGCGATTGGATTCAATCATGGAGAG
ACCTGCCTGTATTAATAAATCAGTGGGCCAATGTGGTTCGCTGGGAAAAATCCACAAAACCTTTCTTGAGAACTTCTGAA
TTCCTATGGCAAGAAGGACATACCGCCCATAGAACTGAAGAAGACGCTGAAGAAGAAGCTTTACAAATGCTTGATGTATA
TAAGGATTTTGTTGAAAATGACATGGCAATTCCTGTGTTAAATGGCTTGAAATCGGAAAAAGAAAAGTTTGCTGGAGCTC
TGCGAACTTTCTGTATTGAGGCCTTGATGTCAGATGGTAGAGCTTTACAAGCCGGTACCAGTCACAATTTGGGTCAACAC
TTTGCTAAGGTATTCGATATCACCTTCTTAGATCAGGATGATGAGCGGAAATATGTTTGGCAGACTTCATGGGGTGTTTC
AACCAGGTTAATAGGTGCATTGATTATGGTACATGGTGATAACCGTGGCCTAAAAATTCCACCAAAAGTAGCACCACACC
AGCTGGTAATGGTTCCAATTACACCTAAAAAACAGCGTGAACAGGTATTGGAAGAAAGCGATAAACTATATCAAGAGCTT
AAAGATAAATTCCGAGTTAAATTAGATAACAGAGAAGAACATACTCCTGGTTGGAAATTCAATGAATGGGAAATGAAAGG
AGTACCTATCAGGCTTGAAATTGGACCTAAAGACATAGAAAAAGATCAAGTTGTCCTTGTAAGACGGGATACAGATGAAA
AAATGTTTGTTAAACGTGATGAACTAATAGATAAACTTGAGGAATTAATTGAGGATATTCAAAACAAAATGCTTCAAACA
GCTAAGAACTTTTTAGAAGAAAATACCCATACAGCTTCGAGTTTAGATGAACTAGGACAAATTCTGGAACAAAAACGAGG
AATGATTAAGGCTTATTGGTGTGGAAATCAAGCATGTGAAGAAAAGGTTAAAGATGATACCAAAGCCACAATAAGAGTTA
TTCCTTTTGAGGCTGAAACAGGGGGTAGCTGTATCGCATGTGGCTATCATAATGACGATAATAAGGAAGTGTTTTTTGCC
AGGGCATATTAA

Upstream 100 bases:

>100_bases
GAGTTAGTATTCTGAAGTTGATAACTCTAAATCTAAGTTATCAACAATCAACTGAAACAAAGTTTAAATTTATAAGACTT
TGAAACTAAGGAGGGATCAT

Downstream 100 bases:

>100_bases
GTCAAAACACTTGATAACTAATAGACTTAATAACTAATAACTATGATCTTAGGGATTTTAAAACAGTGATGATGGGGAAG
TCCTACTAAATATAATAAGG

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS

Number of amino acids: Translated: 483; Mature: 482

Protein sequence:

>483_residues
MSKNNEEFVKEITPQSEDYSQWYLDVIKKTKLVDYAPVKGCMVIRPYGYAIWEKMKEGLDRRIKETGHENAYFPLFIPES
LLQKEADHVEGFAPEVAWITKGGDEELSESLAVRPTSEAMFGEMYSDWIQSWRDLPVLINQWANVVRWEKSTKPFLRTSE
FLWQEGHTAHRTEEDAEEEALQMLDVYKDFVENDMAIPVLNGLKSEKEKFAGALRTFCIEALMSDGRALQAGTSHNLGQH
FAKVFDITFLDQDDERKYVWQTSWGVSTRLIGALIMVHGDNRGLKIPPKVAPHQLVMVPITPKKQREQVLEESDKLYQEL
KDKFRVKLDNREEHTPGWKFNEWEMKGVPIRLEIGPKDIEKDQVVLVRRDTDEKMFVKRDELIDKLEELIEDIQNKMLQT
AKNFLEENTHTASSLDELGQILEQKRGMIKAYWCGNQACEEKVKDDTKATIRVIPFEAETGGSCIACGYHNDDNKEVFFA
RAY

Sequences:

>Translated_483_residues
MSKNNEEFVKEITPQSEDYSQWYLDVIKKTKLVDYAPVKGCMVIRPYGYAIWEKMKEGLDRRIKETGHENAYFPLFIPES
LLQKEADHVEGFAPEVAWITKGGDEELSESLAVRPTSEAMFGEMYSDWIQSWRDLPVLINQWANVVRWEKSTKPFLRTSE
FLWQEGHTAHRTEEDAEEEALQMLDVYKDFVENDMAIPVLNGLKSEKEKFAGALRTFCIEALMSDGRALQAGTSHNLGQH
FAKVFDITFLDQDDERKYVWQTSWGVSTRLIGALIMVHGDNRGLKIPPKVAPHQLVMVPITPKKQREQVLEESDKLYQEL
KDKFRVKLDNREEHTPGWKFNEWEMKGVPIRLEIGPKDIEKDQVVLVRRDTDEKMFVKRDELIDKLEELIEDIQNKMLQT
AKNFLEENTHTASSLDELGQILEQKRGMIKAYWCGNQACEEKVKDDTKATIRVIPFEAETGGSCIACGYHNDDNKEVFFA
RAY
>Mature_482_residues
SKNNEEFVKEITPQSEDYSQWYLDVIKKTKLVDYAPVKGCMVIRPYGYAIWEKMKEGLDRRIKETGHENAYFPLFIPESL
LQKEADHVEGFAPEVAWITKGGDEELSESLAVRPTSEAMFGEMYSDWIQSWRDLPVLINQWANVVRWEKSTKPFLRTSEF
LWQEGHTAHRTEEDAEEEALQMLDVYKDFVENDMAIPVLNGLKSEKEKFAGALRTFCIEALMSDGRALQAGTSHNLGQHF
AKVFDITFLDQDDERKYVWQTSWGVSTRLIGALIMVHGDNRGLKIPPKVAPHQLVMVPITPKKQREQVLEESDKLYQELK
DKFRVKLDNREEHTPGWKFNEWEMKGVPIRLEIGPKDIEKDQVVLVRRDTDEKMFVKRDELIDKLEELIEDIQNKMLQTA
KNFLEENTHTASSLDELGQILEQKRGMIKAYWCGNQACEEKVKDDTKATIRVIPFEAETGGSCIACGYHNDDNKEVFFAR
AY

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro)

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 3 subfamily

Homologues:

Organism=Homo sapiens, GI62241042, Length=494, Percent_Identity=44.331983805668, Blast_Score=429, Evalue=1e-120,
Organism=Escherichia coli, GI1786392, Length=162, Percent_Identity=27.1604938271605, Blast_Score=71, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI71991342, Length=446, Percent_Identity=44.3946188340807, Blast_Score=410, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI71991346, Length=357, Percent_Identity=49.2997198879552, Blast_Score=382, Evalue=1e-106,
Organism=Saccharomyces cerevisiae, GI6321808, Length=482, Percent_Identity=43.5684647302905, Blast_Score=423, Evalue=1e-119,
Organism=Drosophila melanogaster, GI24649468, Length=493, Percent_Identity=43.0020283975659, Blast_Score=410, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24649466, Length=493, Percent_Identity=43.0020283975659, Blast_Score=409, Evalue=1e-114,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): SYP_NATTJ (B2A5T8)

Other databases:

- EMBL:   CP001034
- RefSeq:   YP_001916619.1
- ProteinModelPortal:   B2A5T8
- SMR:   B2A5T8
- GeneID:   6314422
- GenomeReviews:   CP001034_GR
- KEGG:   nth:Nther_0435
- HOGENOM:   HBG334108
- OMA:   PGWKFNE
- ProtClustDB:   PRK08661
- GO:   GO:0005737
- HAMAP:   MF_01571
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004499
- InterPro:   IPR017449
- InterPro:   IPR016061
- Gene3D:   G3DSA:3.40.50.800
- Gene3D:   G3DSA:3.30.110.30
- PANTHER:   PTHR11451:SF6
- PRINTS:   PR01046
- SMART:   SM00946
- TIGRFAMs:   TIGR00408

Pfam domain/function: PF03129 HGTP_anticodon; PF09180 ProRS-C_1; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd; SSF64586 Pro-tRNA_synth_II_C

EC number: =6.1.1.15

Molecular weight: Translated: 55971; Mature: 55840

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKNNEEFVKEITPQSEDYSQWYLDVIKKTKLVDYAPVKGCMVIRPYGYAIWEKMKEGLD
CCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHH
RRIKETGHENAYFPLFIPESLLQKEADHVEGFAPEVAWITKGGDEELSESLAVRPTSEAM
HHHHHCCCCCCEEEEECCHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHCCCCCHHHH
FGEMYSDWIQSWRDLPVLINQWANVVRWEKSTKPFLRTSEFLWQEGHTAHRTEEDAEEEA
HHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHH
LQMLDVYKDFVENDMAIPVLNGLKSEKEKFAGALRTFCIEALMSDGRALQAGTSHNLGQH
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCHHHH
FAKVFDITFLDQDDERKYVWQTSWGVSTRLIGALIMVHGDNRGLKIPPKVAPHQLVMVPI
HHHHHHEEEECCCCCCCEEEEECCCHHHHHHHEEEEEECCCCCCCCCCCCCCCCEEEEEC
TPKKQREQVLEESDKLYQELKDKFRVKLDNREEHTPGWKFNEWEMKGVPIRLEIGPKDIE
CCHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCCEEECCEEEEEEECCCCCC
KDQVVLVRRDTDEKMFVKRDELIDKLEELIEDIQNKMLQTAKNFLEENTHTASSLDELGQ
CCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
ILEQKRGMIKAYWCGNQACEEKVKDDTKATIRVIPFEAETGGSCIACGYHNDDNKEVFFA
HHHHHCCCEEEEECCCHHHHHHHCCCCCCEEEEEEEECCCCCCEEEECCCCCCCCEEEEE
RAY
ECC
>Mature Secondary Structure 
SKNNEEFVKEITPQSEDYSQWYLDVIKKTKLVDYAPVKGCMVIRPYGYAIWEKMKEGLD
CCCHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHH
RRIKETGHENAYFPLFIPESLLQKEADHVEGFAPEVAWITKGGDEELSESLAVRPTSEAM
HHHHHCCCCCCEEEEECCHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHCCCCCHHHH
FGEMYSDWIQSWRDLPVLINQWANVVRWEKSTKPFLRTSEFLWQEGHTAHRTEEDAEEEA
HHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHH
LQMLDVYKDFVENDMAIPVLNGLKSEKEKFAGALRTFCIEALMSDGRALQAGTSHNLGQH
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCHHHH
FAKVFDITFLDQDDERKYVWQTSWGVSTRLIGALIMVHGDNRGLKIPPKVAPHQLVMVPI
HHHHHHEEEECCCCCCCEEEEECCCHHHHHHHEEEEEECCCCCCCCCCCCCCCCEEEEEC
TPKKQREQVLEESDKLYQELKDKFRVKLDNREEHTPGWKFNEWEMKGVPIRLEIGPKDIE
CCHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCCEEECCEEEEEEECCCCCC
KDQVVLVRRDTDEKMFVKRDELIDKLEELIEDIQNKMLQTAKNFLEENTHTASSLDELGQ
CCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
ILEQKRGMIKAYWCGNQACEEKVKDDTKATIRVIPFEAETGGSCIACGYHNDDNKEVFFA
HHHHHCCCEEEEECCCHHHHHHHCCCCCCEEEEEEEECCCCCCEEEECCCCCCCCEEEEE
RAY
ECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA