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The map label for this gene is dagK [H]

Identifier: 188585069

GI number: 188585069

Start: 449511

End: 450407

Strand: Direct

Name: dagK [H]

Synonym: Nther_0430

Alternate gene names: 188585069

Gene position: 449511-450407 (Clockwise)

Preceding gene: 188585067

Following gene: 188585071

Centisome position: 14.2

GC content: 34.45

Gene sequence:

>897_bases
ATGTTTTTGATTGTTTATAATCCCAAGGCTGGTCATGTCCAAAGTCAAAACTTATCGGACTTGACGAGGAAAATTAAAGA
AAGAAATTTTCAAGTTAAATCAATAGCCTTAACTGAGACAGATTTTTATGAACAACTGATGGATAATTTGACAAAGACTC
GATGGCTAATATCTTTTGGCGGTGATGGGACTTTAAATACATTGATAAGTTATTTGTCACCTTTAGCTGATGAGAAATTG
CCTGTATTGATTCCCTATCCTTCTGGAACAGCTAATGATTTTTCCTATCAGTTATATGGGGGTACTCCAGAAATAGAGCA
AATTTTAACCGCTGTGGAAAGGGAAGAATATTGTTATTTAGATGTGGGGAAAGTCAATGACAATTATTTTATTAATGTTC
TTGGTGCGGGAATTTTTGTTGATGTAGCTTATAAAACAGATTCAGCTTTGAAACAACGGATCGGCATGTGGGCGTATTGG
TTCGAAGCTATTAAGCAAATTCCTAATTTTAAACCTATAACATTTTTTATAGATGATGGGGTTAATTGTCAAGAAATTGA
AGGTTATTTGCTTCTTGTACTAAACGGGAAAGGAGCCGGGGGCTTTAAAAGCCTAGCACCTGAATCTTCTCTGAGAGACG
GTAAATTGGATCTGGTTGTAATTAAGGAATCAAGAACTTTATTATCCCTTGCCTCTTTATTCCCTAAAATATTACGAGGT
GATCATCTGGATGATCATCGGGTTACTTATATACAAAAAAACACTTTTAAAATTAAATTAGATAATAAAACCGATAGTGA
TGTTGTTTGTGTTGTAGATGGTGAAAAGGGACCCGATTTTCCTTTGGAAGTACAGGTTTTAAATCAGAAGTTACCCTTTT
TGCATTGTCAATATTAA

Upstream 100 bases:

>100_bases
GAAATTCCATGTTAAGATTCACCTCGTTTACCTATTTTATGAATCAATCACTAAAATTGTATCATATACCTTGTATTTTG
ATAATAGGAGGAAAATAAAT

Downstream 100 bases:

>100_bases
AGTAATTAATATTTTAACTGTCTCCTTAAGTTATTTATTTCAACAGTTTTTCCAGTAATTCTTGATTGTTCTGCCGCAAA
AGCTGTAATATGGCTTTCTA

Product: diacylglycerol kinase catalytic region

Products: NA

Alternate protein names: DAG kinase; DAGK [H]

Number of amino acids: Translated: 298; Mature: 298

Protein sequence:

>298_residues
MFLIVYNPKAGHVQSQNLSDLTRKIKERNFQVKSIALTETDFYEQLMDNLTKTRWLISFGGDGTLNTLISYLSPLADEKL
PVLIPYPSGTANDFSYQLYGGTPEIEQILTAVEREEYCYLDVGKVNDNYFINVLGAGIFVDVAYKTDSALKQRIGMWAYW
FEAIKQIPNFKPITFFIDDGVNCQEIEGYLLLVLNGKGAGGFKSLAPESSLRDGKLDLVVIKESRTLLSLASLFPKILRG
DHLDDHRVTYIQKNTFKIKLDNKTDSDVVCVVDGEKGPDFPLEVQVLNQKLPFLHCQY

Sequences:

>Translated_298_residues
MFLIVYNPKAGHVQSQNLSDLTRKIKERNFQVKSIALTETDFYEQLMDNLTKTRWLISFGGDGTLNTLISYLSPLADEKL
PVLIPYPSGTANDFSYQLYGGTPEIEQILTAVEREEYCYLDVGKVNDNYFINVLGAGIFVDVAYKTDSALKQRIGMWAYW
FEAIKQIPNFKPITFFIDDGVNCQEIEGYLLLVLNGKGAGGFKSLAPESSLRDGKLDLVVIKESRTLLSLASLFPKILRG
DHLDDHRVTYIQKNTFKIKLDNKTDSDVVCVVDGEKGPDFPLEVQVLNQKLPFLHCQY
>Mature_298_residues
MFLIVYNPKAGHVQSQNLSDLTRKIKERNFQVKSIALTETDFYEQLMDNLTKTRWLISFGGDGTLNTLISYLSPLADEKL
PVLIPYPSGTANDFSYQLYGGTPEIEQILTAVEREEYCYLDVGKVNDNYFINVLGAGIFVDVAYKTDSALKQRIGMWAYW
FEAIKQIPNFKPITFFIDDGVNCQEIEGYLLLVLNGKGAGGFKSLAPESSLRDGKLDLVVIKESRTLLSLASLFPKILRG
DHLDDHRVTYIQKNTFKIKLDNKTDSDVVCVVDGEKGPDFPLEVQVLNQKLPFLHCQY

Specific function: Catalyzes the phosphorylation of diacylglycerol (DAG) into phosphatidic acid. Is a key enzyme involved in the production of lipoteichoic acid by reintroducing DAG formed from the breakdown of membrane phospholipids into the phosphatidylglycerol biosynthet

COG id: COG1597

COG function: function code IR; Sphingosine kinase and enzymes related to eukaryotic diacylglycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DAGKc domain [H]

Homologues:

Organism=Escherichia coli, GI1788402, Length=243, Percent_Identity=26.3374485596708, Blast_Score=68, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017438
- InterPro:   IPR005218
- InterPro:   IPR001206 [H]

Pfam domain/function: PF00781 DAGK_cat [H]

EC number: =2.7.1.107 [H]

Molecular weight: Translated: 33699; Mature: 33699

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFLIVYNPKAGHVQSQNLSDLTRKIKERNFQVKSIALTETDFYEQLMDNLTKTRWLISFG
CEEEEECCCCCCCCCCCHHHHHHHHHHCCCEEEEEEEECHHHHHHHHHHHHHEEEEEEEC
GDGTLNTLISYLSPLADEKLPVLIPYPSGTANDFSYQLYGGTPEIEQILTAVEREEYCYL
CCCHHHHHHHHHHHHCCCCCCEEEECCCCCCCCEEEEEECCCHHHHHHHHHHCCCCEEEE
DVGKVNDNYFINVLGAGIFVDVAYKTDSALKQRIGMWAYWFEAIKQIPNFKPITFFIDDG
EECCCCCCEEEEEEECCEEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECC
VNCQEIEGYLLLVLNGKGAGGFKSLAPESSLRDGKLDLVVIKESRTLLSLASLFPKILRG
CCCEEECCEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHCC
DHLDDHRVTYIQKNTFKIKLDNKTDSDVVCVVDGEKGPDFPLEVQVLNQKLPFLHCQY
CCCCCCEEEEEECCEEEEEECCCCCCCEEEEEECCCCCCCCEEEEEECCCCCEEEECC
>Mature Secondary Structure
MFLIVYNPKAGHVQSQNLSDLTRKIKERNFQVKSIALTETDFYEQLMDNLTKTRWLISFG
CEEEEECCCCCCCCCCCHHHHHHHHHHCCCEEEEEEEECHHHHHHHHHHHHHEEEEEEEC
GDGTLNTLISYLSPLADEKLPVLIPYPSGTANDFSYQLYGGTPEIEQILTAVEREEYCYL
CCCHHHHHHHHHHHHCCCCCCEEEECCCCCCCCEEEEEECCCHHHHHHHHHHCCCCEEEE
DVGKVNDNYFINVLGAGIFVDVAYKTDSALKQRIGMWAYWFEAIKQIPNFKPITFFIDDG
EECCCCCCEEEEEEECCEEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECC
VNCQEIEGYLLLVLNGKGAGGFKSLAPESSLRDGKLDLVVIKESRTLLSLASLFPKILRG
CCCEEECCEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHCC
DHLDDHRVTYIQKNTFKIKLDNKTDSDVVCVVDGEKGPDFPLEVQVLNQKLPFLHCQY
CCCCCCEEEEEECCEEEEEECCCCCCCEEEEEECCCCCCCCEEEEEECCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]