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The map label for this gene is pmbA [C]

Identifier: 188585035

GI number: 188585035

Start: 411957

End: 413267

Strand: Reverse

Name: pmbA [C]

Synonym: Nther_0396

Alternate gene names: 188585035

Gene position: 413267-411957 (Counterclockwise)

Preceding gene: 188585036

Following gene: 188585034

Centisome position: 13.06

GC content: 37.76

Gene sequence:

>1311_bases
ATGGATAAACTGCTCTCTTTAGCAAAGAAAAAAAACGAACAAGCAGAACTGTTTGAGGTGTCTGTAACCAGGACTCCAGT
TGAATTCGAAAATAACAAACTTAAAAACATAAAAACCAAAGAACAAAAATCCACAGCACTAAGGTTGATCTCTCCAGATA
ATCGCCTGGGTTTTTGCACTACTACTTCTACTGAAGACAAAGCTGAAGATCTGTTAAACAAAGCCAGAGAAACCAGTCAG
TTTGGAAGAAGTTTTCAACCCAGTTTACCTAAAGTTAATCCTGAAGAATTAGATAATATGTACGATCAAGATGTTATGGA
TTTACCTACTGAAAAAATGATAGAAATGGGCGAAGAAATAGTAGATAGTATTAGAAGTAAGAATTCCGAAGCTTTAGCCA
GTGCCAGTATTGAAAAAACGCAATTACAGGTGAAAATCAAAAATTCCGAGGGATTAAATACCGAGTACGAAAGAACTGCC
TTTTCCGTAAGAGGTGGAGCTATGTTATTAGTAGGAAAAAGTTTTTTGCGTATCGGTCAAGGTAAAAAATTACCCCGGCT
ATTCACTGACCTGACTGCTTTAAAAAATGAATTGATGCAGGACTTACATTACGGTTCTAAAGAAAAAGAGATCAACAGTG
GCAGCTACAAAGTAATTTTTGCCCCGGAGGCCATGAGCGATTTTATGCGACCAATACTGGCCAGCCTTAACGGTCAAAGT
GTAGAAAAAGAAGTTTCACCATTTAAAGATAAACTCGACCAACAGCTTTTTGATGAAAAAGTGAATTTAACTGATATGCC
TCATCGTCCATTTTCGCCGGGAATGTGTCCTGTTGACGATGAGGGAGTACCAACAAAAGAAACAGACCTAATAAAGAATG
GAACTATTAAAACTTTCCCGGTCGATCTATTAACAGCTCATTCTCTAGGTGTAGAACCTACGGGACATGGTATCAGACAA
AGCCCTTATTCTTTACCCGCTCCGGATTTACACACATGTGTCCTGTCACCAGGTGAAATGGCTTATCACGATATGATAGG
AGAGTTAGATCGAGGGATTATCATAAAAGATTTAATGGGTGCTTGGGCAGGTAATCCTTACAGCGGTGAAGTTAATGGCA
ATATTTCTTTGGGCTACTTAGTTGAAAACGGAAAAATTATTGGAAGAATTAAAGATTGCATGGTAGCTGTCAATGTATTT
GAAGCTTTACAAAATCAGCTAGTTTCCTTTAGCCAGGAGGCTAAATGGATAGGTAATAGATTATTCCCTTACGCATTACT
AGAAGGTATAGACATAACTGCTAAAAATTAA

Upstream 100 bases:

>100_bases
ACTGGCGGTGGATGTGGAAAAGGTGAGCAAGGTCCCCTCCCAACCAGTCAAGGCTCACCTTCGATCAGGATTCAGAATGT
AATTATAGGAGGGAAATCCG

Downstream 100 bases:

>100_bases
ATATAACCTGATTTACAAAAGCTGTTCCCTTGTTAAACTGGGGAACAGCTTTTTTTCTTTACTTCGATTTATTTCCCATA
GTCCTGACATTATCTGTAAC

Product: peptidase U62 modulator of DNA gyrase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 436; Mature: 436

Protein sequence:

>436_residues
MDKLLSLAKKKNEQAELFEVSVTRTPVEFENNKLKNIKTKEQKSTALRLISPDNRLGFCTTTSTEDKAEDLLNKARETSQ
FGRSFQPSLPKVNPEELDNMYDQDVMDLPTEKMIEMGEEIVDSIRSKNSEALASASIEKTQLQVKIKNSEGLNTEYERTA
FSVRGGAMLLVGKSFLRIGQGKKLPRLFTDLTALKNELMQDLHYGSKEKEINSGSYKVIFAPEAMSDFMRPILASLNGQS
VEKEVSPFKDKLDQQLFDEKVNLTDMPHRPFSPGMCPVDDEGVPTKETDLIKNGTIKTFPVDLLTAHSLGVEPTGHGIRQ
SPYSLPAPDLHTCVLSPGEMAYHDMIGELDRGIIIKDLMGAWAGNPYSGEVNGNISLGYLVENGKIIGRIKDCMVAVNVF
EALQNQLVSFSQEAKWIGNRLFPYALLEGIDITAKN

Sequences:

>Translated_436_residues
MDKLLSLAKKKNEQAELFEVSVTRTPVEFENNKLKNIKTKEQKSTALRLISPDNRLGFCTTTSTEDKAEDLLNKARETSQ
FGRSFQPSLPKVNPEELDNMYDQDVMDLPTEKMIEMGEEIVDSIRSKNSEALASASIEKTQLQVKIKNSEGLNTEYERTA
FSVRGGAMLLVGKSFLRIGQGKKLPRLFTDLTALKNELMQDLHYGSKEKEINSGSYKVIFAPEAMSDFMRPILASLNGQS
VEKEVSPFKDKLDQQLFDEKVNLTDMPHRPFSPGMCPVDDEGVPTKETDLIKNGTIKTFPVDLLTAHSLGVEPTGHGIRQ
SPYSLPAPDLHTCVLSPGEMAYHDMIGELDRGIIIKDLMGAWAGNPYSGEVNGNISLGYLVENGKIIGRIKDCMVAVNVF
EALQNQLVSFSQEAKWIGNRLFPYALLEGIDITAKN
>Mature_436_residues
MDKLLSLAKKKNEQAELFEVSVTRTPVEFENNKLKNIKTKEQKSTALRLISPDNRLGFCTTTSTEDKAEDLLNKARETSQ
FGRSFQPSLPKVNPEELDNMYDQDVMDLPTEKMIEMGEEIVDSIRSKNSEALASASIEKTQLQVKIKNSEGLNTEYERTA
FSVRGGAMLLVGKSFLRIGQGKKLPRLFTDLTALKNELMQDLHYGSKEKEINSGSYKVIFAPEAMSDFMRPILASLNGQS
VEKEVSPFKDKLDQQLFDEKVNLTDMPHRPFSPGMCPVDDEGVPTKETDLIKNGTIKTFPVDLLTAHSLGVEPTGHGIRQ
SPYSLPAPDLHTCVLSPGEMAYHDMIGELDRGIIIKDLMGAWAGNPYSGEVNGNISLGYLVENGKIIGRIKDCMVAVNVF
EALQNQLVSFSQEAKWIGNRLFPYALLEGIDITAKN

Specific function: May Facilitate The Secretion Of The Peptide Antibiotic Microcin B17 (Mccb17) By Completing Its Maturation. Suppresses The Inhibitory Activity Of The Carbon Storage Regulator (Csra). [C]

COG id: COG0312

COG function: function code R; Predicted Zn-dependent proteases and their inactivated homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790682, Length=192, Percent_Identity=25.5208333333333, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1789640, Length=170, Percent_Identity=28.2352941176471, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002510 [H]

Pfam domain/function: PF01523 PmbA_TldD [H]

EC number: NA

Molecular weight: Translated: 48514; Mature: 48514

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKLLSLAKKKNEQAELFEVSVTRTPVEFENNKLKNIKTKEQKSTALRLISPDNRLGFCT
CCHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCCHHHHCCEEEEECCCCCEEEEE
TTSTEDKAEDLLNKARETSQFGRSFQPSLPKVNPEELDNMYDQDVMDLPTEKMIEMGEEI
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHH
VDSIRSKNSEALASASIEKTQLQVKIKNSEGLNTEYERTAFSVRGGAMLLVGKSFLRIGQ
HHHHHCCCCHHHHHHCCEEEEEEEEEECCCCCCCHHHHHEEEECCCEEEEECHHHHHHCC
GKKLPRLFTDLTALKNELMQDLHYGSKEKEINSGSYKVIFAPEAMSDFMRPILASLNGQS
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHCCCCH
VEKEVSPFKDKLDQQLFDEKVNLTDMPHRPFSPGMCPVDDEGVPTKETDLIKNGTIKTFP
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCEEEEC
VDLLTAHSLGVEPTGHGIRQSPYSLPAPDLHTCVLSPGEMAYHDMIGELDRGIIIKDLMG
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHEEEECCCHHHHHHHHHHHCCCCHHHHHHH
AWAGNPYSGEVNGNISLGYLVENGKIIGRIKDCMVAVNVFEALQNQLVSFSQEAKWIGNR
HHCCCCCCCEECCCEEEEEEEECCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LFPYALLEGIDITAKN
CHHHHHHCCCCEEECC
>Mature Secondary Structure
MDKLLSLAKKKNEQAELFEVSVTRTPVEFENNKLKNIKTKEQKSTALRLISPDNRLGFCT
CCHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCCHHHHCCEEEEECCCCCEEEEE
TTSTEDKAEDLLNKARETSQFGRSFQPSLPKVNPEELDNMYDQDVMDLPTEKMIEMGEEI
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHH
VDSIRSKNSEALASASIEKTQLQVKIKNSEGLNTEYERTAFSVRGGAMLLVGKSFLRIGQ
HHHHHCCCCHHHHHHCCEEEEEEEEEECCCCCCCHHHHHEEEECCCEEEEECHHHHHHCC
GKKLPRLFTDLTALKNELMQDLHYGSKEKEINSGSYKVIFAPEAMSDFMRPILASLNGQS
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHCCCCH
VEKEVSPFKDKLDQQLFDEKVNLTDMPHRPFSPGMCPVDDEGVPTKETDLIKNGTIKTFP
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCEEEEC
VDLLTAHSLGVEPTGHGIRQSPYSLPAPDLHTCVLSPGEMAYHDMIGELDRGIIIKDLMG
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHEEEECCCHHHHHHHHHHHCCCCHHHHHHH
AWAGNPYSGEVNGNISLGYLVENGKIIGRIKDCMVAVNVFEALQNQLVSFSQEAKWIGNR
HHCCCCCCCEECCCEEEEEEEECCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LFPYALLEGIDITAKN
CHHHHHHCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]