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The map label for this gene is pcm

Identifier: 188584955

GI number: 188584955

Start: 330821

End: 331453

Strand: Reverse

Name: pcm

Synonym: Nther_0314

Alternate gene names: 188584955

Gene position: 331453-330821 (Counterclockwise)

Preceding gene: 188584956

Following gene: 188584939

Centisome position: 10.47

GC content: 36.97

Gene sequence:

>633_bases
ATGAAAGATAAGTTTTCAGCACAAAGAGAAAAAATGGTCCAAAAGCAGTTAGCCAAAAGGGATATTGAAGATCCCAAAGT
TTTGGATGCATTTAGGAAAGTTCCCCGGGAAGAATTTATAACAGAGTCTAAGCAGCATCAAGCATACGGGGACCATCCTT
TACCAATAGGTCATGGTCAAACCATATCCCAACCTTATATTGTTGCCATGATGACACAAGCTTTAGAACCAAGTTCTCAT
GACATAGTCTTAGAAGTAGGTACAGGTTCTGGTTACCAAACGGCTATTTTAGCTGAGCTAACCAAAAAAGTATTTTCCCT
TGAAAGAATTCCTGAGTTAAGTGAACAGGCTGGGCGTATTCTATCTAAACTAGGTTATCAAAATATTGTTTTAAAAATAT
CTGATGATGACAACGATCTAACAATTCATGAACAAAAAGAATTTGATAAAATAATCGTAACTGCAGCTGCCTCCCAAATT
CCTCCTGAATTAACACAACAGTTAAAAGAAGGAGGAACCCTGGTGATACCAGTAGGAGAACTTATGTTTCAAGAACTACT
AGTGGTCACTAAACATAATGGAGAATTAAGGAAGAAAAGTTTAGGGGGATGCAGGTTTGTTCCATTAAAGTAA

Upstream 100 bases:

>100_bases
AAAAGCCTTGACGCAAATATAGCACTAGTAAAAAATAATGCAAGAAAAGCAGCTGAACTGGCCTGTGAATATAATTACCT
AGAGCAGTGAGGTGTATATC

Downstream 100 bases:

>100_bases
AACCTCCAGTTTAGCTAATAAAGACCCCAATCCTGTAATATAGGGATGGGGTCTTTTATATGGAAAGCTAAGTTCAGTTG
TAATTAGCATCTCCAGATAA

Product: protein-L-isoaspartate O-methyltransferase

Products: NA

Alternate protein names: L-isoaspartyl protein carboxyl methyltransferase; Protein L-isoaspartyl methyltransferase; Protein-beta-aspartate methyltransferase; PIMT

Number of amino acids: Translated: 210; Mature: 210

Protein sequence:

>210_residues
MKDKFSAQREKMVQKQLAKRDIEDPKVLDAFRKVPREEFITESKQHQAYGDHPLPIGHGQTISQPYIVAMMTQALEPSSH
DIVLEVGTGSGYQTAILAELTKKVFSLERIPELSEQAGRILSKLGYQNIVLKISDDDNDLTIHEQKEFDKIIVTAAASQI
PPELTQQLKEGGTLVIPVGELMFQELLVVTKHNGELRKKSLGGCRFVPLK

Sequences:

>Translated_210_residues
MKDKFSAQREKMVQKQLAKRDIEDPKVLDAFRKVPREEFITESKQHQAYGDHPLPIGHGQTISQPYIVAMMTQALEPSSH
DIVLEVGTGSGYQTAILAELTKKVFSLERIPELSEQAGRILSKLGYQNIVLKISDDDNDLTIHEQKEFDKIIVTAAASQI
PPELTQQLKEGGTLVIPVGELMFQELLVVTKHNGELRKKSLGGCRFVPLK
>Mature_210_residues
MKDKFSAQREKMVQKQLAKRDIEDPKVLDAFRKVPREEFITESKQHQAYGDHPLPIGHGQTISQPYIVAMMTQALEPSSH
DIVLEVGTGSGYQTAILAELTKKVFSLERIPELSEQAGRILSKLGYQNIVLKISDDDNDLTIHEQKEFDKIIVTAAASQI
PPELTQQLKEGGTLVIPVGELMFQELLVVTKHNGELRKKSLGGCRFVPLK

Specific function: Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins

COG id: COG2518

COG function: function code O; Protein-L-isoaspartate carboxylmethyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. L- isoaspartyl/D-aspartyl protein methyltransferase family

Homologues:

Organism=Homo sapiens, GI226530908, Length=189, Percent_Identity=31.7460317460317, Blast_Score=68, Evalue=4e-12,
Organism=Escherichia coli, GI1789100, Length=203, Percent_Identity=46.3054187192118, Blast_Score=160, Evalue=8e-41,
Organism=Caenorhabditis elegans, GI71983477, Length=198, Percent_Identity=29.7979797979798, Blast_Score=74, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI193207222, Length=199, Percent_Identity=28.1407035175879, Blast_Score=64, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PIMT_NATTJ (B2A578)

Other databases:

- EMBL:   CP001034
- RefSeq:   YP_001916500.1
- ProteinModelPortal:   B2A578
- SMR:   B2A578
- GeneID:   6316147
- GenomeReviews:   CP001034_GR
- KEGG:   nth:Nther_0314
- HOGENOM:   HBG699907
- OMA:   GYHAAVV
- GO:   GO:0005737
- HAMAP:   MF_00090
- InterPro:   IPR000682
- PANTHER:   PTHR11579
- TIGRFAMs:   TIGR00080

Pfam domain/function: PF01135 PCMT

EC number: =2.1.1.77

Molecular weight: Translated: 23515; Mature: 23515

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS01279 PCMT

Important sites: ACT_SITE 63-63

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDKFSAQREKMVQKQLAKRDIEDPKVLDAFRKVPREEFITESKQHQAYGDHPLPIGHGQ
CCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHCCHHHHCCCCCCCCCCCCC
TISQPYIVAMMTQALEPSSHDIVLEVGTGSGYQTAILAELTKKVFSLERIPELSEQAGRI
CCCCCHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHH
LSKLGYQNIVLKISDDDNDLTIHEQKEFDKIIVTAAASQIPPELTQQLKEGGTLVIPVGE
HHHCCCCEEEEEEECCCCCEEEECHHHHHHHHHHHHHHHCCHHHHHHHHCCCEEEEEHHH
LMFQELLVVTKHNGELRKKSLGGCRFVPLK
HHHHHHHHHHCCCCHHHHHCCCCEEEECCC
>Mature Secondary Structure
MKDKFSAQREKMVQKQLAKRDIEDPKVLDAFRKVPREEFITESKQHQAYGDHPLPIGHGQ
CCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHCCHHHHCCCCCCCCCCCCC
TISQPYIVAMMTQALEPSSHDIVLEVGTGSGYQTAILAELTKKVFSLERIPELSEQAGRI
CCCCCHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHH
LSKLGYQNIVLKISDDDNDLTIHEQKEFDKIIVTAAASQIPPELTQQLKEGGTLVIPVGE
HHHCCCCEEEEEEECCCCCEEEECHHHHHHHHHHHHHHHCCHHHHHHHHCCCEEEEEHHH
LMFQELLVVTKHNGELRKKSLGGCRFVPLK
HHHHHHHHHHCCCCHHHHHCCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA