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The map label for this gene is sigX [H]

Identifier: 188584774

GI number: 188584774

Start: 157410

End: 157973

Strand: Reverse

Name: sigX [H]

Synonym: Nther_0132

Alternate gene names: 188584774

Gene position: 157973-157410 (Counterclockwise)

Preceding gene: 188584775

Following gene: 188584773

Centisome position: 4.99

GC content: 30.85

Gene sequence:

>564_bases
TTGAAGGAGCTGTCAAAACAACAATTGGCGGAATTAATATCAAAAGCTAGTCATAATGAACAAGCTTTTGAACAGCTATA
CAATTATTTCTTTCCAAAAATTTATCAATACATTTATTATCGAGTAAATAATAAAGAAGTAACAGAGGATCTTGTATCTG
ATATTTTCCTTAAAGTTTTACGAAATTTGAAATCTTTGAAAAAGCCCGAAGATTTTCAAAGCTGGATATATTCTATTGCA
CGTAATGCCCTGATAGATTACTACAGAAAAAAGGGTAGAAAAAAAGAAGAGGCTTCTGAAGCTATATCCGATGATGAAAC
AGTTCTGATAGATACACCCGAAGATCATGTATTACTAGAAGAACATTCAAAAGAAATTTTTAAACTCCTTGAAATTTTAT
CTATAGAACAACAGGAAGTTATTCAATTAAGATTTGTAGATGAATTGAAAATTAAAGAAATTGCCAAAGTATTAGATAAA
AGTGAAGGAGCTATTAAAGGTCTTTTATATCGCGGACTAAATAAACTATCCCGCCACCTAGACAGTAAGGAGGTGCAATC
ATGA

Upstream 100 bases:

>100_bases
CCACTCTTAAAAAATAAAGTATCGGAGTTGGCACAACTTTTAATAGATTAGTGTCGTCTTATAATGATGCCAGCTCCTTG
TAAGAAAGGATGATTTCCAA

Downstream 100 bases:

>100_bases
GCACCGACGGTAAAAATAATAACAAAAAGCAAGATTTAAATACACAAAATAACTGTGACATCATCACCAATGAAATCAAA
GAAGAGATAAAAGATTTAAA

Product: RNA polymerase, sigma-24 subunit, ECF subfamily

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 187; Mature: 187

Protein sequence:

>187_residues
MKELSKQQLAELISKASHNEQAFEQLYNYFFPKIYQYIYYRVNNKEVTEDLVSDIFLKVLRNLKSLKKPEDFQSWIYSIA
RNALIDYYRKKGRKKEEASEAISDDETVLIDTPEDHVLLEEHSKEIFKLLEILSIEQQEVIQLRFVDELKIKEIAKVLDK
SEGAIKGLLYRGLNKLSRHLDSKEVQS

Sequences:

>Translated_187_residues
MKELSKQQLAELISKASHNEQAFEQLYNYFFPKIYQYIYYRVNNKEVTEDLVSDIFLKVLRNLKSLKKPEDFQSWIYSIA
RNALIDYYRKKGRKKEEASEAISDDETVLIDTPEDHVLLEEHSKEIFKLLEILSIEQQEVIQLRFVDELKIKEIAKVLDK
SEGAIKGLLYRGLNKLSRHLDSKEVQS
>Mature_187_residues
MKELSKQQLAELISKASHNEQAFEQLYNYFFPKIYQYIYYRVNNKEVTEDLVSDIFLKVLRNLKSLKKPEDFQSWIYSIA
RNALIDYYRKKGRKKEEASEAISDDETVLIDTPEDHVLLEEHSKEIFKLLEILSIEQQEVIQLRFVDELKIKEIAKVLDK
SEGAIKGLLYRGLNKLSRHLDSKEVQS

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. May be involved in the regulation of iron metabolism [H]

COG id: COG1595

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788926, Length=178, Percent_Identity=28.0898876404494, Blast_Score=66, Evalue=1e-12,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2 [H]

EC number: NA

Molecular weight: Translated: 22089; Mature: 22089

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
0.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKELSKQQLAELISKASHNEQAFEQLYNYFFPKIYQYIYYRVNNKEVTEDLVSDIFLKVL
CCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
RNLKSLKKPEDFQSWIYSIARNALIDYYRKKGRKKEEASEAISDDETVLIDTPEDHVLLE
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCEEEEECCCCHHHHH
EHSKEIFKLLEILSIEQQEVIQLRFVDELKIKEIAKVLDKSEGAIKGLLYRGLNKLSRHL
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHC
DSKEVQS
CHHHCCC
>Mature Secondary Structure
MKELSKQQLAELISKASHNEQAFEQLYNYFFPKIYQYIYYRVNNKEVTEDLVSDIFLKVL
CCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
RNLKSLKKPEDFQSWIYSIARNALIDYYRKKGRKKEEASEAISDDETVLIDTPEDHVLLE
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCEEEEECCCCHHHHH
EHSKEIFKLLEILSIEQQEVIQLRFVDELKIKEIAKVLDKSEGAIKGLLYRGLNKLSRHL
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHC
DSKEVQS
CHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7934829; 9384377; 8052622; 9393439 [H]