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The map label for this gene is leuS

Identifier: 188528338

GI number: 188528338

Start: 1567946

End: 1570366

Strand: Direct

Name: leuS

Synonym: HPSH_08050

Alternate gene names: 188528338

Gene position: 1567946-1570366 (Clockwise)

Preceding gene: 188528337

Following gene: 188528339

Centisome position: 97.48

GC content: 40.19

Gene sequence:

>2421_bases
ATGGATTTTATCAATATAGAAAAAAAATGGCAAGAATTTTGGTGGAAAAATAAGAGTTTTGAACCTAAAGACGATTTTAA
CCTCCCTAAAAAATACATTTTGAGCATGCTGCCTTATCCTAGTGGGGAAATCCACATGGGGCATGTGCGCAATTACACCA
TTGGCGACGCTTTGGCGCGTTATTATCGTTTGCACCATTATAATGTGTTACACCCTATGGGGTTTGATTCTTTTGGGATG
CCTGCAGAAAATGCGGCCATTAAGCATGGTATCCACCCTAAAACCTGGACTTATGAAAACATTGAAAACATGCAAAAAGA
GTTTGAAACTTTAGGGTTTTCTTTTTCTAAAAATAGGGAATTTGCCACTTCAGATCCGGATTACACGAAATTTGAACAGC
AATTTTTCATTGACTTGTGGGAAAAAGGGCTAGTCTATCGCAAGAAAGCCATGCTCAATTGGTGCCCTAACGATAAAACC
GTTTTAGCTAATGAGCAAGTCATTGATGGGAGGTGTTGGCGTTGCGATACAGAAGTTATTCAAAAAGAACTCTATCAATA
TTATTTGAAGATCACAAATTACGCTGAAGAATTGCTCAAAGACTTAAAGGCTTTAGAAAACCATTGGCCTTCTCAAGTCC
TAATTATGCAAAAAAACTGGATAGGGAAATCTAGCGGGTTGCAATTTGGTTTTAAAATCGCTGATGAGTGCTTGAAGGCT
TGCAATGGCATTCAAGAAATTGAAGTTTTTACCACAAGAGCGGACACCATTTATGGCATAACTTTCATCGCCATCGCCCC
AGAACACCCTTTAGTAGAGCATGCCATTAAGCAAGTGAGCCAAGAAGATTCAAAGATGATTAAAGCGATTTTAAACACGA
CTCAAAGAGAAAGAGCTTTAGAGAAAAAAGGGGCGTTTTTAGGCATTTACGCTATCCACCCTTTAACAAAGCAAAAAATC
CCTGTGTGGGTGGCTAATTTCGCCCTAGCTCATTATGGCTCTGGGGCGTTAATGGGCGTGCCAGCCTGCGATGAAAGGGA
TTTTGAATTCGCTAATCTTTATCATATCCCTATTAAAGTGATCACTCAAAGCCCTCAAAATTTGCCCCACACCAAAGAAG
AGGTTTTAAAAAATAGCGGGGAGTGGAGTGATCTTTCTAGCTCAGTGGCCAGAGAAAAAATCATTGCTTATTTTGAAAAA
GAAAACCTCGGTAAAAGGGTGATCAACTACCGCTTGCAAGATTGGGGAGTGAGCCGTCAAAGGTATTGGGGAGCGCCTAT
TCCTATGATTCATTGCAAACATTGCGGGATTGTGCCTGAAACCCAACTGCCGGTAACTTTGCCTGAAGACATTGTGATTG
ATGGGGAGGGCAATCCGTTAGAAAAGCATGCGAGTTGGAAATTCGCTCAATGCCCTAAATGCCACAAAAACGCTTTAAGA
GAAACAGACACTATGGATACTTTCATCCAATCTAGTTGGTATTTCTTGCGCTACACCACGCCCAAAAATCAGCGCGAAAA
TCAAGCGTTTGATCAAAATTACTTGAAGTATTTCATGCCAGTGGACACTTACATTGGCGGCATTGAACATGCGATTTTGC
ACTTGTTATATGCGCGCTTTTTCACTAAGGCTTTAAGGGATTTGGGCTATCTTAATTTAGATGAGCCTTTTAAACAGCTT
ATCACTCAAGGCATGGTCTTAAAAGATGGCGCTAAGATGAGCAAATCTAAAGGCAATGTCGTTAGCCCTAAAGAGATACT
TAAAAAATACGGGGCCGATGCGGCGAGGCTTTTTATCCTTTTTGCTGCCCCACCGGCTAAAGAGTTAGAATGGAATGACA
ACGCTTTAGAAGGGGCGCACCGGTTTATCAAGCGCTTATACGACAAAGCGAGCGCTATTAACCCCACTACTTCTAAGCCT
GAATTTAAAGAAGTCAGCCTGAATGAAGCGGAAAAATTAGGGCGTAAAAAAGTCTATGAAGCGTTAAAAAAATCGCATGA
AATTTTCAATAAGGCTGAAAGCGCTTATTCGTTTAACACTTTGATCGCAAGTTGCATGGAAGCTTTAAACGCTTTGAGCG
CGCAAAATAATGAGCGGATTTTATGCGAGGGTTATTTTGTGTTGTTGCAAATTTTAGAGCCTATTATCCCGCACACGGCA
TGGGAGTTGAGCGAGAGGCTTTTTAAAAGAGAGAATTTTAAGCCTATAGCGATCGATGAAAGCGCTTTTGTGGAAGACTT
TATGACTTTAGGGCTTACCATTAATGGCAAAAGGCGCGCGGAATTGAAAGTTAGTATTAACGCTGATAAAGAAGAAATCA
TTATTTTGGCTAAAAAAGAATTAGAGAAATATTTAGAAAATGCGAGCGTTAAAAAAGAAATTTATGTGCCTAATAAGCTT
GTTAATTTTGTTATCGCATGA

Upstream 100 bases:

>100_bases
ATACATGCTCATGATTGGGGCGTTGATTAGCAACGCTTTCAGCCTTATCTTTTTGTTGGTTGAAAGCGTTGTAACGAGCG
AAACGGATTAAGGGGTAGCG

Downstream 100 bases:

>100_bases
GGGCTTTACTTTTTTTTATTTTGCTATTTTTGTTCAAGGGCTGTGGGTATAAGCCCATCGCCGCTTACGCTCAAAACGCT
TTAGGCGATAGCATATACGT

Product: leucyl-tRNA synthetase

Products: NA

Alternate protein names: Leucine--tRNA ligase; LeuRS [H]

Number of amino acids: Translated: 806; Mature: 806

Protein sequence:

>806_residues
MDFINIEKKWQEFWWKNKSFEPKDDFNLPKKYILSMLPYPSGEIHMGHVRNYTIGDALARYYRLHHYNVLHPMGFDSFGM
PAENAAIKHGIHPKTWTYENIENMQKEFETLGFSFSKNREFATSDPDYTKFEQQFFIDLWEKGLVYRKKAMLNWCPNDKT
VLANEQVIDGRCWRCDTEVIQKELYQYYLKITNYAEELLKDLKALENHWPSQVLIMQKNWIGKSSGLQFGFKIADECLKA
CNGIQEIEVFTTRADTIYGITFIAIAPEHPLVEHAIKQVSQEDSKMIKAILNTTQRERALEKKGAFLGIYAIHPLTKQKI
PVWVANFALAHYGSGALMGVPACDERDFEFANLYHIPIKVITQSPQNLPHTKEEVLKNSGEWSDLSSSVAREKIIAYFEK
ENLGKRVINYRLQDWGVSRQRYWGAPIPMIHCKHCGIVPETQLPVTLPEDIVIDGEGNPLEKHASWKFAQCPKCHKNALR
ETDTMDTFIQSSWYFLRYTTPKNQRENQAFDQNYLKYFMPVDTYIGGIEHAILHLLYARFFTKALRDLGYLNLDEPFKQL
ITQGMVLKDGAKMSKSKGNVVSPKEILKKYGADAARLFILFAAPPAKELEWNDNALEGAHRFIKRLYDKASAINPTTSKP
EFKEVSLNEAEKLGRKKVYEALKKSHEIFNKAESAYSFNTLIASCMEALNALSAQNNERILCEGYFVLLQILEPIIPHTA
WELSERLFKRENFKPIAIDESAFVEDFMTLGLTINGKRRAELKVSINADKEEIIILAKKELEKYLENASVKKEIYVPNKL
VNFVIA

Sequences:

>Translated_806_residues
MDFINIEKKWQEFWWKNKSFEPKDDFNLPKKYILSMLPYPSGEIHMGHVRNYTIGDALARYYRLHHYNVLHPMGFDSFGM
PAENAAIKHGIHPKTWTYENIENMQKEFETLGFSFSKNREFATSDPDYTKFEQQFFIDLWEKGLVYRKKAMLNWCPNDKT
VLANEQVIDGRCWRCDTEVIQKELYQYYLKITNYAEELLKDLKALENHWPSQVLIMQKNWIGKSSGLQFGFKIADECLKA
CNGIQEIEVFTTRADTIYGITFIAIAPEHPLVEHAIKQVSQEDSKMIKAILNTTQRERALEKKGAFLGIYAIHPLTKQKI
PVWVANFALAHYGSGALMGVPACDERDFEFANLYHIPIKVITQSPQNLPHTKEEVLKNSGEWSDLSSSVAREKIIAYFEK
ENLGKRVINYRLQDWGVSRQRYWGAPIPMIHCKHCGIVPETQLPVTLPEDIVIDGEGNPLEKHASWKFAQCPKCHKNALR
ETDTMDTFIQSSWYFLRYTTPKNQRENQAFDQNYLKYFMPVDTYIGGIEHAILHLLYARFFTKALRDLGYLNLDEPFKQL
ITQGMVLKDGAKMSKSKGNVVSPKEILKKYGADAARLFILFAAPPAKELEWNDNALEGAHRFIKRLYDKASAINPTTSKP
EFKEVSLNEAEKLGRKKVYEALKKSHEIFNKAESAYSFNTLIASCMEALNALSAQNNERILCEGYFVLLQILEPIIPHTA
WELSERLFKRENFKPIAIDESAFVEDFMTLGLTINGKRRAELKVSINADKEEIIILAKKELEKYLENASVKKEIYVPNKL
VNFVIA
>Mature_806_residues
MDFINIEKKWQEFWWKNKSFEPKDDFNLPKKYILSMLPYPSGEIHMGHVRNYTIGDALARYYRLHHYNVLHPMGFDSFGM
PAENAAIKHGIHPKTWTYENIENMQKEFETLGFSFSKNREFATSDPDYTKFEQQFFIDLWEKGLVYRKKAMLNWCPNDKT
VLANEQVIDGRCWRCDTEVIQKELYQYYLKITNYAEELLKDLKALENHWPSQVLIMQKNWIGKSSGLQFGFKIADECLKA
CNGIQEIEVFTTRADTIYGITFIAIAPEHPLVEHAIKQVSQEDSKMIKAILNTTQRERALEKKGAFLGIYAIHPLTKQKI
PVWVANFALAHYGSGALMGVPACDERDFEFANLYHIPIKVITQSPQNLPHTKEEVLKNSGEWSDLSSSVAREKIIAYFEK
ENLGKRVINYRLQDWGVSRQRYWGAPIPMIHCKHCGIVPETQLPVTLPEDIVIDGEGNPLEKHASWKFAQCPKCHKNALR
ETDTMDTFIQSSWYFLRYTTPKNQRENQAFDQNYLKYFMPVDTYIGGIEHAILHLLYARFFTKALRDLGYLNLDEPFKQL
ITQGMVLKDGAKMSKSKGNVVSPKEILKKYGADAARLFILFAAPPAKELEWNDNALEGAHRFIKRLYDKASAINPTTSKP
EFKEVSLNEAEKLGRKKVYEALKKSHEIFNKAESAYSFNTLIASCMEALNALSAQNNERILCEGYFVLLQILEPIIPHTA
WELSERLFKRENFKPIAIDESAFVEDFMTLGLTINGKRRAELKVSINADKEEIIILAKKELEKYLENASVKKEIYVPNKL
VNFVIA

Specific function: Unknown

COG id: COG0495

COG function: function code J; Leucyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI7661872, Length=878, Percent_Identity=33.4851936218679, Blast_Score=455, Evalue=1e-128,
Organism=Escherichia coli, GI1786861, Length=868, Percent_Identity=42.5115207373272, Blast_Score=687, Evalue=0.0,
Organism=Escherichia coli, GI1790708, Length=413, Percent_Identity=23.2445520581114, Blast_Score=92, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI71997510, Length=730, Percent_Identity=30.958904109589, Blast_Score=298, Evalue=9e-81,
Organism=Caenorhabditis elegans, GI71997517, Length=730, Percent_Identity=30.958904109589, Blast_Score=298, Evalue=1e-80,
Organism=Caenorhabditis elegans, GI212645227, Length=273, Percent_Identity=30.7692307692308, Blast_Score=115, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6323414, Length=804, Percent_Identity=37.8109452736318, Blast_Score=506, Evalue=1e-144,
Organism=Saccharomyces cerevisiae, GI6321531, Length=876, Percent_Identity=20.662100456621, Blast_Score=104, Evalue=6e-23,
Organism=Drosophila melanogaster, GI21355409, Length=839, Percent_Identity=31.5852205005959, Blast_Score=373, Evalue=1e-103,
Organism=Drosophila melanogaster, GI281366294, Length=141, Percent_Identity=29.7872340425532, Blast_Score=69, Evalue=1e-11,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002300
- InterPro:   IPR002302
- InterPro:   IPR014729
- InterPro:   IPR009080
- InterPro:   IPR009008 [H]

Pfam domain/function: PF00133 tRNA-synt_1 [H]

EC number: =6.1.1.4 [H]

Molecular weight: Translated: 93140; Mature: 93140

Theoretical pI: Translated: 7.90; Mature: 7.90

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFINIEKKWQEFWWKNKSFEPKDDFNLPKKYILSMLPYPSGEIHMGHVRNYTIGDALAR
CCCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCCCCEEECCCCCCCHHHHHHH
YYRLHHYNVLHPMGFDSFGMPAENAAIKHGIHPKTWTYENIENMQKEFETLGFSFSKNRE
HHHHHHCCEECCCCCCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCC
FATSDPDYTKFEQQFFIDLWEKGLVYRKKAMLNWCPNDKTVLANEQVIDGRCWRCDTEVI
CCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCEEEECCCEECCCCCCCCHHHH
QKELYQYYLKITNYAEELLKDLKALENHWPSQVLIMQKNWIGKSSGLQFGFKIADECLKA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHH
CNGIQEIEVFTTRADTIYGITFIAIAPEHPLVEHAIKQVSQEDSKMIKAILNTTQRERAL
HCCCEEEEEEEECCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EKKGAFLGIYAIHPLTKQKIPVWVANFALAHYGSGALMGVPACDERDFEFANLYHIPIKV
HHCCCEEEEEEECCCCCCCCCHHHHHHHHHHCCCCCEECCCCCCCCCCCEEEEEEEEEEE
ITQSPQNLPHTKEEVLKNSGEWSDLSSSVAREKIIAYFEKENLGKRVINYRLQDWGVSRQ
EECCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
RYWGAPIPMIHCKHCGIVPETQLPVTLPEDIVIDGEGNPLEKHASWKFAQCPKCHKNALR
HCCCCCCCEEEECCCCCCCCCCCCEECCCCEEECCCCCCCHHHCCCCCCCCCHHHHHHHH
ETDTMDTFIQSSWYFLRYTTPKNQRENQAFDQNYLKYFMPVDTYIGGIEHAILHLLYARF
HHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FTKALRDLGYLNLDEPFKQLITQGMVLKDGAKMSKSKGNVVSPKEILKKYGADAARLFIL
HHHHHHHCCCCCCCHHHHHHHHCCCEEECCCHHCCCCCCCCCHHHHHHHHCCCCEEEEEE
FAAPPAKELEWNDNALEGAHRFIKRLYDKASAINPTTSKPEFKEVSLNEAEKLGRKKVYE
EECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHCCCHHHHHHHHHHHHH
ALKKSHEIFNKAESAYSFNTLIASCMEALNALSAQNNERILCEGYFVLLQILEPIIPHTA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHCCHH
WELSERLFKRENFKPIAIDESAFVEDFMTLGLTINGKRRAELKVSINADKEEIIILAKKE
HHHHHHHHHHCCCCCEEECHHHHHHHHHHHCEEECCCEEEEEEEEECCCCCCEEEEEHHH
LEKYLENASVKKEIYVPNKLVNFVIA
HHHHHHCCCCCEEEECCHHHHHHHCC
>Mature Secondary Structure
MDFINIEKKWQEFWWKNKSFEPKDDFNLPKKYILSMLPYPSGEIHMGHVRNYTIGDALAR
CCCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCCCCEEECCCCCCCHHHHHHH
YYRLHHYNVLHPMGFDSFGMPAENAAIKHGIHPKTWTYENIENMQKEFETLGFSFSKNRE
HHHHHHCCEECCCCCCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCC
FATSDPDYTKFEQQFFIDLWEKGLVYRKKAMLNWCPNDKTVLANEQVIDGRCWRCDTEVI
CCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCEEEECCCEECCCCCCCCHHHH
QKELYQYYLKITNYAEELLKDLKALENHWPSQVLIMQKNWIGKSSGLQFGFKIADECLKA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHH
CNGIQEIEVFTTRADTIYGITFIAIAPEHPLVEHAIKQVSQEDSKMIKAILNTTQRERAL
HCCCEEEEEEEECCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EKKGAFLGIYAIHPLTKQKIPVWVANFALAHYGSGALMGVPACDERDFEFANLYHIPIKV
HHCCCEEEEEEECCCCCCCCCHHHHHHHHHHCCCCCEECCCCCCCCCCCEEEEEEEEEEE
ITQSPQNLPHTKEEVLKNSGEWSDLSSSVAREKIIAYFEKENLGKRVINYRLQDWGVSRQ
EECCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
RYWGAPIPMIHCKHCGIVPETQLPVTLPEDIVIDGEGNPLEKHASWKFAQCPKCHKNALR
HCCCCCCCEEEECCCCCCCCCCCCEECCCCEEECCCCCCCHHHCCCCCCCCCHHHHHHHH
ETDTMDTFIQSSWYFLRYTTPKNQRENQAFDQNYLKYFMPVDTYIGGIEHAILHLLYARF
HHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FTKALRDLGYLNLDEPFKQLITQGMVLKDGAKMSKSKGNVVSPKEILKKYGADAARLFIL
HHHHHHHCCCCCCCHHHHHHHHCCCEEECCCHHCCCCCCCCCHHHHHHHHCCCCEEEEEE
FAAPPAKELEWNDNALEGAHRFIKRLYDKASAINPTTSKPEFKEVSLNEAEKLGRKKVYE
EECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHCCCHHHHHHHHHHHHH
ALKKSHEIFNKAESAYSFNTLIASCMEALNALSAQNNERILCEGYFVLLQILEPIIPHTA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHCCHH
WELSERLFKRENFKPIAIDESAFVEDFMTLGLTINGKRRAELKVSINADKEEIIILAKKE
HHHHHHHHHHCCCCCEEECHHHHHHHHHHHCEEECCCEEEEEEEEECCCCCCEEEEEHHH
LEKYLENASVKKEIYVPNKLVNFVIA
HHHHHHCCCCCEEEECCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA