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The map label for this gene is ppnK [H]

Identifier: 188528315

GI number: 188528315

Start: 1548173

End: 1549027

Strand: Direct

Name: ppnK [H]

Synonym: HPSH_07935

Alternate gene names: 188528315

Gene position: 1548173-1549027 (Clockwise)

Preceding gene: 188528313

Following gene: 188528316

Centisome position: 96.25

GC content: 40.23

Gene sequence:

>855_bases
ATGAAAGATTCACATCAAACTATCGGCGTGTTTGTGCGGCCTACTCATTATCAAAACCCTCTTTTTGAAAAGCTAGAACG
AGCTAAAGAATGGGTTTTAAAGCTTTTAGAAGATGAAGGGTTTGAAAGCTTTATGATTGATGGCCTTGATGGAGCAAAAG
ATGAACGATTGATAGAAAAAGCCTATGCGTTTTTATGTTTAGGGGGCGATGGCACGATTTTAGGGGCTTTAAGAATGACG
CATTCTTACAATAAGCCATGTTTTGGGGTGAGAATAGGGAATTTAGGGTTTTTGAGCGCGGTTGAATTGAATGGTTTGAA
AGGTTTCTTACAAGATCTCAAGCAAGATAAGATCAAATTAGAAGAGCATTTGGCTTTAGAGGGCCGTATTGGAAAAACCT
CTTTTTATGCGATCAATGAAATTGTGATTGCCAAAAAAAAAGCTTTAGGGGTTTTAGACATCCAAGCTTATGTGGGCCAT
ACGCCCTTTAACACCTATAAAGGCGATGGGCTTATCATTGCTACGCCCCTAGGCTCGACCGCTTATAATTTGAGCGCTCA
TGGGCCTATTGTGCATGCTTTAAGCCAGAGCTATATTTTAACGCCCTTGTGCGATTTTTCTTTAACGCAACGCCCTTTAG
TGTTAGGAGCGGAATTTTGTTTGAATTTTTGCGCTCATGAAGACGCTCTTGTGGTCATTGATGGGCAAGCCACCTATGAT
TTAAAGGCCAACCAACCCCTATACATTCAAAAAAGCCCCACGACCACCAAGCTCTTACAAAAAAATTCAAGGGATTATTT
TAAAGTGCTTAAAGAAAAGCTCTTATGGGGAGAAAGCCCTAGCAAAAAAAGATAA

Upstream 100 bases:

>100_bases
AAAATAAAAACTCCTTATGAATTCCACATAAGATTAATAAAAAGCGGCTAAAACCCTTTTTTTTAAAATTTTGTTTCAAG
CTTTAAGCTACAATATACGC

Downstream 100 bases:

>100_bases
AAAGGGTAAAACATGCGAGATTTTAATAACGCTCAAATCACACGCTTAAAAGTGCGTCAAAACGCTGTTTTTGAAAAATT
GGATCTAGAGTTTAAAGACG

Product: inorganic polyphosphate/ATP-NAD kinase

Products: NA

Alternate protein names: Poly(P)/ATP NAD kinase [H]

Number of amino acids: Translated: 284; Mature: 284

Protein sequence:

>284_residues
MKDSHQTIGVFVRPTHYQNPLFEKLERAKEWVLKLLEDEGFESFMIDGLDGAKDERLIEKAYAFLCLGGDGTILGALRMT
HSYNKPCFGVRIGNLGFLSAVELNGLKGFLQDLKQDKIKLEEHLALEGRIGKTSFYAINEIVIAKKKALGVLDIQAYVGH
TPFNTYKGDGLIIATPLGSTAYNLSAHGPIVHALSQSYILTPLCDFSLTQRPLVLGAEFCLNFCAHEDALVVIDGQATYD
LKANQPLYIQKSPTTTKLLQKNSRDYFKVLKEKLLWGESPSKKR

Sequences:

>Translated_284_residues
MKDSHQTIGVFVRPTHYQNPLFEKLERAKEWVLKLLEDEGFESFMIDGLDGAKDERLIEKAYAFLCLGGDGTILGALRMT
HSYNKPCFGVRIGNLGFLSAVELNGLKGFLQDLKQDKIKLEEHLALEGRIGKTSFYAINEIVIAKKKALGVLDIQAYVGH
TPFNTYKGDGLIIATPLGSTAYNLSAHGPIVHALSQSYILTPLCDFSLTQRPLVLGAEFCLNFCAHEDALVVIDGQATYD
LKANQPLYIQKSPTTTKLLQKNSRDYFKVLKEKLLWGESPSKKR
>Mature_284_residues
MKDSHQTIGVFVRPTHYQNPLFEKLERAKEWVLKLLEDEGFESFMIDGLDGAKDERLIEKAYAFLCLGGDGTILGALRMT
HSYNKPCFGVRIGNLGFLSAVELNGLKGFLQDLKQDKIKLEEHLALEGRIGKTSFYAINEIVIAKKKALGVLDIQAYVGH
TPFNTYKGDGLIIATPLGSTAYNLSAHGPIVHALSQSYILTPLCDFSLTQRPLVLGAEFCLNFCAHEDALVVIDGQATYD
LKANQPLYIQKSPTTTKLLQKNSRDYFKVLKEKLLWGESPSKKR

Specific function: Catalyzes the phosphorylation of NAD to NADP. Utilizes ATP and other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus [H]

COG id: COG0061

COG function: function code G; Predicted sugar kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD kinase family [H]

Homologues:

Organism=Homo sapiens, GI55743112, Length=250, Percent_Identity=25.2, Blast_Score=75, Evalue=7e-14,
Organism=Escherichia coli, GI1788968, Length=222, Percent_Identity=30.1801801801802, Blast_Score=103, Evalue=9e-24,
Organism=Saccharomyces cerevisiae, GI6320794, Length=244, Percent_Identity=25, Blast_Score=98, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6325068, Length=169, Percent_Identity=30.7692307692308, Blast_Score=87, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6322509, Length=237, Percent_Identity=26.1603375527426, Blast_Score=83, Evalue=4e-17,
Organism=Drosophila melanogaster, GI28573828, Length=257, Percent_Identity=27.2373540856031, Blast_Score=81, Evalue=8e-16,
Organism=Drosophila melanogaster, GI161077047, Length=257, Percent_Identity=27.2373540856031, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI28573830, Length=263, Percent_Identity=26.9961977186312, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI28573832, Length=257, Percent_Identity=27.2373540856031, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI28573826, Length=263, Percent_Identity=26.9961977186312, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI281363321, Length=282, Percent_Identity=25.177304964539, Blast_Score=68, Evalue=6e-12,
Organism=Drosophila melanogaster, GI20129957, Length=282, Percent_Identity=25.177304964539, Blast_Score=68, Evalue=7e-12,
Organism=Drosophila melanogaster, GI281363323, Length=282, Percent_Identity=25.177304964539, Blast_Score=68, Evalue=7e-12,
Organism=Drosophila melanogaster, GI24653424, Length=288, Percent_Identity=25, Blast_Score=68, Evalue=8e-12,
Organism=Drosophila melanogaster, GI24653422, Length=282, Percent_Identity=25.177304964539, Blast_Score=67, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016064
- InterPro:   IPR017438
- InterPro:   IPR017437
- InterPro:   IPR002504 [H]

Pfam domain/function: PF01513 NAD_kinase [H]

EC number: =2.7.1.23 [H]

Molecular weight: Translated: 31668; Mature: 31668

Theoretical pI: Translated: 8.20; Mature: 8.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDSHQTIGVFVRPTHYQNPLFEKLERAKEWVLKLLEDEGFESFMIDGLDGAKDERLIEK
CCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHH
AYAFLCLGGDGTILGALRMTHSYNKPCFGVRIGNLGFLSAVELNGLKGFLQDLKQDKIKL
HEEEEEECCCCEEEEEEEHHCCCCCCEEEEEECCCCEEEEEEHHHHHHHHHHHHHHHHHH
EEHLALEGRIGKTSFYAINEIVIAKKKALGVLDIQAYVGHTPFNTYKGDGLIIATPLGST
HHHEEECCCCCCCHHEEHHHHEEEHHHHCCEEEEHHHHCCCCCCCCCCCCEEEEECCCCC
AYNLSAHGPIVHALSQSYILTPLCDFSLTQRPLVLGAEFCLNFCAHEDALVVIDGQATYD
EEECCCCCHHHHHHCCCEEECCCCCCCCCCCCCEEEHHHHHHHHCCCCEEEEECCCEEEE
LKANQPLYIQKSPTTTKLLQKNSRDYFKVLKEKLLWGESPSKKR
ECCCCCEEEECCCCHHHHHHCCCHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MKDSHQTIGVFVRPTHYQNPLFEKLERAKEWVLKLLEDEGFESFMIDGLDGAKDERLIEK
CCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHH
AYAFLCLGGDGTILGALRMTHSYNKPCFGVRIGNLGFLSAVELNGLKGFLQDLKQDKIKL
HEEEEEECCCCEEEEEEEHHCCCCCCEEEEEECCCCEEEEEEHHHHHHHHHHHHHHHHHH
EEHLALEGRIGKTSFYAINEIVIAKKKALGVLDIQAYVGHTPFNTYKGDGLIIATPLGST
HHHEEECCCCCCCHHEEHHHHEEEHHHHCCEEEEHHHHCCCCCCCCCCCCEEEEECCCCC
AYNLSAHGPIVHALSQSYILTPLCDFSLTQRPLVLGAEFCLNFCAHEDALVVIDGQATYD
EEECCCCCHHHHHHCCCEEECCCCCCCCCCCCCEEEHHHHHHHHCCCCEEEEECCCEEEE
LKANQPLYIQKSPTTTKLLQKNSRDYFKVLKEKLLWGESPSKKR
ECCCCCEEEECCCCHHHHHHCCCHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA