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The map label for this gene is murE

Identifier: 188528265

GI number: 188528265

Start: 1486622

End: 1487965

Strand: Direct

Name: murE

Synonym: HPSH_07655

Alternate gene names: 188528265

Gene position: 1486622-1487965 (Clockwise)

Preceding gene: 188528264

Following gene: 188528266

Centisome position: 92.42

GC content: 37.72

Gene sequence:

>1344_bases
ATGAAGCTTAAAAAAACCCTGACTTATCAAAACCACACCTATTCTTTTTTAAGCGATAACACGCATGAAGTTTTAGAAAA
CCCTAAAGGAATCCTTTTTGTCAAAACGCCTTTAAATGAAAAATACTCCCCTTTCATTGCAGAAAAAAACCTGGCTATTT
TAGATTTTAACGAGCTTAAAAACTATTTTGATTTTAAAATGAAAATTGTAGGGATTACTGGCACTAATGGCAAAACGACC
ACAGCGAGTTTGATGTATTCCTTACTCTTAGATTTGAATAAAAAGACCGCCCTTTTAGGCACAAGAGGGTTTTTTATCAA
CGACAAACAAATCAAAGAAAAGGGTTTGACCACGCCCACTCTTTTAGAGCTTTATAGCGATTTAGAAGAAGCGATGCGTT
TAAAATGCGAATACTTTATTATGGAAGTGAGCTCCCATGCGATTGTCCAAAAGCGCATTGCCGGGCTTGATTTTGCCCTT
AAAATCCTCACTAATATCACAAGCGATCATTTAGATTTCCACGAAAGCATAGAAAATTACAAAGACGCCAAAAACAGCTT
TTTTAAAGATGAGGGCTTGAAAGTGATTAACAGAGATGAAACAAACTCCCTTTTTAACCCCATTAACGCGCACACTTACG
CGCTGGATAAAAAAGCGCATTTAAACATTCAAGCCTTTTCGCTCACCCCCTCCATTAGCGCATCTTTATGCTACCAACAA
GATTTAAGAAATCCTAATCTCAAAGAAATCGCCCTCATCCATTCCCCCCTTTTAGGGCGTTACAACCTTTATAATATCTT
AGCGGGCGTTTTAGGGGTCAAATTACTCACCCAATTACCGCTAGAAGCGATTGCGCCGCTATTAGAAAACTTTTATGGGG
TTAAGGGGCGTTTGGAAATTGTGCATTCTAAACCTTTAGTGATCGTGGATTTTGCCCACACCACAGACGGCATGCAACAA
GTCTTTGAAAGCTTTAAAAACCAAAAAATCACCGCTCTTTTTGGAGCAGGGGGCGATAGGGACAAGACCAAGCGCCCTAA
AATGGGAGAGGTAGCGAGTTATTACGCTCATAAAATTATCTTAACTTCAGACAACCCCAGAAGCGAAAACGAAGAAGACA
TTATTAAGGATATTTTAAAAGGCATCAGCGATTCTTCTAAAGTGGTTATAGAAAAAGACCGAAAAAAAGCCATTTTAAAC
GCTTTAGAAAATTTAAAAGACGATGAGGTGTTATTGATTTTAGGCAAGGGCGATGAAAACACTCAAATCTTTAAAGACAA
AACGATTTTTTTTAGCGACCAGGAAGTAATTAAAAGCTATTACCAACATTTAAAACAAGGATAA

Upstream 100 bases:

>100_bases
GATAAATTACATTGAAAACATGCATGAAGATTCTTTTATTTACGATAATTTTTTGCGCTCTCTTTTAGAAGACGCTCTCA
AATCCAATAAGGCTTAAAGA

Downstream 100 bases:

>100_bases
AACATGCAAGAATTCAGTTTGTGGTGCGATTTTATAGAAAGGGATTTTTTAGAAAACGACTTTTTAAAGCTCATTAATAA
GGGGGCTATTTGTGGGGCGA

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]

Number of amino acids: Translated: 447; Mature: 447

Protein sequence:

>447_residues
MKLKKTLTYQNHTYSFLSDNTHEVLENPKGILFVKTPLNEKYSPFIAEKNLAILDFNELKNYFDFKMKIVGITGTNGKTT
TASLMYSLLLDLNKKTALLGTRGFFINDKQIKEKGLTTPTLLELYSDLEEAMRLKCEYFIMEVSSHAIVQKRIAGLDFAL
KILTNITSDHLDFHESIENYKDAKNSFFKDEGLKVINRDETNSLFNPINAHTYALDKKAHLNIQAFSLTPSISASLCYQQ
DLRNPNLKEIALIHSPLLGRYNLYNILAGVLGVKLLTQLPLEAIAPLLENFYGVKGRLEIVHSKPLVIVDFAHTTDGMQQ
VFESFKNQKITALFGAGGDRDKTKRPKMGEVASYYAHKIILTSDNPRSENEEDIIKDILKGISDSSKVVIEKDRKKAILN
ALENLKDDEVLLILGKGDENTQIFKDKTIFFSDQEVIKSYYQHLKQG

Sequences:

>Translated_447_residues
MKLKKTLTYQNHTYSFLSDNTHEVLENPKGILFVKTPLNEKYSPFIAEKNLAILDFNELKNYFDFKMKIVGITGTNGKTT
TASLMYSLLLDLNKKTALLGTRGFFINDKQIKEKGLTTPTLLELYSDLEEAMRLKCEYFIMEVSSHAIVQKRIAGLDFAL
KILTNITSDHLDFHESIENYKDAKNSFFKDEGLKVINRDETNSLFNPINAHTYALDKKAHLNIQAFSLTPSISASLCYQQ
DLRNPNLKEIALIHSPLLGRYNLYNILAGVLGVKLLTQLPLEAIAPLLENFYGVKGRLEIVHSKPLVIVDFAHTTDGMQQ
VFESFKNQKITALFGAGGDRDKTKRPKMGEVASYYAHKIILTSDNPRSENEEDIIKDILKGISDSSKVVIEKDRKKAILN
ALENLKDDEVLLILGKGDENTQIFKDKTIFFSDQEVIKSYYQHLKQG
>Mature_447_residues
MKLKKTLTYQNHTYSFLSDNTHEVLENPKGILFVKTPLNEKYSPFIAEKNLAILDFNELKNYFDFKMKIVGITGTNGKTT
TASLMYSLLLDLNKKTALLGTRGFFINDKQIKEKGLTTPTLLELYSDLEEAMRLKCEYFIMEVSSHAIVQKRIAGLDFAL
KILTNITSDHLDFHESIENYKDAKNSFFKDEGLKVINRDETNSLFNPINAHTYALDKKAHLNIQAFSLTPSISASLCYQQ
DLRNPNLKEIALIHSPLLGRYNLYNILAGVLGVKLLTQLPLEAIAPLLENFYGVKGRLEIVHSKPLVIVDFAHTTDGMQQ
VFESFKNQKITALFGAGGDRDKTKRPKMGEVASYYAHKIILTSDNPRSENEEDIIKDILKGISDSSKVVIEKDRKKAILN
ALENLKDDEVLLILGKGDENTQIFKDKTIFFSDQEVIKSYYQHLKQG

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=370, Percent_Identity=30.8108108108108, Blast_Score=166, Evalue=2e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR005761 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.13 [H]

Molecular weight: Translated: 50715; Mature: 50715

Theoretical pI: Translated: 8.19; Mature: 8.19

Prosite motif: PS01011 FOLYLPOLYGLU_SYNT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLKKTLTYQNHTYSFLSDNTHEVLENPKGILFVKTPLNEKYSPFIAEKNLAILDFNELK
CCCCCCEEECCCEEEECCCCCHHHHCCCCCEEEEECCCCCCCCCCEECCCEEEEEHHHHH
NYFDFKMKIVGITGTNGKTTTASLMYSLLLDLNKKTALLGTRGFFINDKQIKEKGLTTPT
HHEEEEEEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEECCCEEECCHHHHHCCCCCHH
LLELYSDLEEAMRLKCEYFIMEVSSHAIVQKRIAGLDFALKILTNITSDHLDFHESIENY
HHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHCHHHHHHHHHHCCHHHHHHHHHHHHH
KDAKNSFFKDEGLKVINRDETNSLFNPINAHTYALDKKAHLNIQAFSLTPSISASLCYQQ
HHHHHHHHHHCCCEEECCCCCHHHCCCCCCCEEEECCCCEEEEEEEEECCCCHHHHHHHH
DLRNPNLKEIALIHSPLLGRYNLYNILAGVLGVKLLTQLPLEAIAPLLENFYGVKGRLEI
HCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCEEEE
VHSKPLVIVDFAHTTDGMQQVFESFKNQKITALFGAGGDRDKTKRPKMGEVASYYAHKII
EECCCEEEEEECCCCHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHEEEE
LTSDNPRSENEEDIIKDILKGISDSSKVVIEKDRKKAILNALENLKDDEVLLILGKGDEN
EECCCCCCCCHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHCCCCCCEEEEEECCCCC
TQIFKDKTIFFSDQEVIKSYYQHLKQG
CEEEECCEEEECCHHHHHHHHHHHHCC
>Mature Secondary Structure
MKLKKTLTYQNHTYSFLSDNTHEVLENPKGILFVKTPLNEKYSPFIAEKNLAILDFNELK
CCCCCCEEECCCEEEECCCCCHHHHCCCCCEEEEECCCCCCCCCCEECCCEEEEEHHHHH
NYFDFKMKIVGITGTNGKTTTASLMYSLLLDLNKKTALLGTRGFFINDKQIKEKGLTTPT
HHEEEEEEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEECCCEEECCHHHHHCCCCCHH
LLELYSDLEEAMRLKCEYFIMEVSSHAIVQKRIAGLDFALKILTNITSDHLDFHESIENY
HHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHCHHHHHHHHHHCCHHHHHHHHHHHHH
KDAKNSFFKDEGLKVINRDETNSLFNPINAHTYALDKKAHLNIQAFSLTPSISASLCYQQ
HHHHHHHHHHCCCEEECCCCCHHHCCCCCCCEEEECCCCEEEEEEEEECCCCHHHHHHHH
DLRNPNLKEIALIHSPLLGRYNLYNILAGVLGVKLLTQLPLEAIAPLLENFYGVKGRLEI
HCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCEEEE
VHSKPLVIVDFAHTTDGMQQVFESFKNQKITALFGAGGDRDKTKRPKMGEVASYYAHKII
EECCCEEEEEECCCCHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHEEEE
LTSDNPRSENEEDIIKDILKGISDSSKVVIEKDRKKAILNALENLKDDEVLLILGKGDEN
EECCCCCCCCHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHCCCCCCEEEEEECCCCC
TQIFKDKTIFFSDQEVIKSYYQHLKQG
CEEEECCEEEECCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]