Click here to switch to the map view.

The map label for this gene is miaA

Identifier: 188528181

GI number: 188528181

Start: 1406665

End: 1407495

Strand: Direct

Name: miaA

Synonym: HPSH_07210

Alternate gene names: 188528181

Gene position: 1406665-1407495 (Clockwise)

Preceding gene: 188528180

Following gene: 188528182

Centisome position: 87.45

GC content: 38.51

Gene sequence:

>831_bases
TTGGACGCTGAAATCTTTTCTTTGGATTCTTTGAGTATTTATAAAGACATTAATATCGCTTCGGCTAAACCGAGCTTGAA
AGAACGAAAAAATATCAAGCATTACGCCCTAGATTACCTTAGCATTGATGAAAAAAATAACGCTCCCCTTTTTAAAACCC
TTTTAGAGGACGCTATGAGGGTGTCTTCTAAAGAGGTATTACTCATTGTGGGGGGGAGCAGTTTTTATCTCAAATCCATT
TTAGAAGGTTTGAGCGATACGCCAAAAATTAGCGGTGAGGAGGCTGTAAAAATAGAGCAAGAAATCAACTCTTTGGCTAA
CCCTTATGCATTCTTAAAATCCATTGACCCCACAAGCGCTTTTAAAATCCATCCAAACGACACTTACCGCATCCATAAGG
CTTTAGAAATCTTTTATTCCACCCACACGCCCCCAAGCGAGTATTTTAAAACTAACCCTAAAAAACCCTTTGAGCATGCC
ATCTCATTATTCGCTCTTTCTATTGAAAAAAGCGCGCTTGCAAACAACATCAAACAGCGCACCAAGAACATGCTTGATTG
TGGCCTTATAGAAGAAATCAAAGCCCTTTATGCTCAATACCCTAAAGATTCGCAGCCTTTTAAAGCCATAGGCGTTAAAG
AGAGCATTCTTTTTTTAGAAAAACAACTCACTTTAAAGAAGCTAGAAGAAGCGATTATCTCTAACACCATCCAATTAGCC
AAGCGCCAAAACACTTTCAATAAAACCCAATTCAATAACCTTTACACAGGAAGCGTTGGAGAAGTTAGGCATGCGATTTT
AAACCACTCAAAAAGCGCTATTAAAGGATAA

Upstream 100 bases:

>100_bases
AAGAGGGAGAGCGTTCATCAAAACGCCTAAGAAACTCATCGCGCTTCTAGGGCCTAGCGGGAGCGGGAAAAGCGCTCTTT
CCATTGAATTAGCCCAAGAA

Downstream 100 bases:

>100_bases
TCTAATGGATGCGCAAACTTTACAAAATCAAATTATCCCTATTTTTATGAGTTTTGATAAGAATTACGCGCTAGGGGCTG
GCGTGAGCCTTTATTCTTTA

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase [H]

Number of amino acids: Translated: 276; Mature: 276

Protein sequence:

>276_residues
MDAEIFSLDSLSIYKDINIASAKPSLKERKNIKHYALDYLSIDEKNNAPLFKTLLEDAMRVSSKEVLLIVGGSSFYLKSI
LEGLSDTPKISGEEAVKIEQEINSLANPYAFLKSIDPTSAFKIHPNDTYRIHKALEIFYSTHTPPSEYFKTNPKKPFEHA
ISLFALSIEKSALANNIKQRTKNMLDCGLIEEIKALYAQYPKDSQPFKAIGVKESILFLEKQLTLKKLEEAIISNTIQLA
KRQNTFNKTQFNNLYTGSVGEVRHAILNHSKSAIKG

Sequences:

>Translated_276_residues
MDAEIFSLDSLSIYKDINIASAKPSLKERKNIKHYALDYLSIDEKNNAPLFKTLLEDAMRVSSKEVLLIVGGSSFYLKSI
LEGLSDTPKISGEEAVKIEQEINSLANPYAFLKSIDPTSAFKIHPNDTYRIHKALEIFYSTHTPPSEYFKTNPKKPFEHA
ISLFALSIEKSALANNIKQRTKNMLDCGLIEEIKALYAQYPKDSQPFKAIGVKESILFLEKQLTLKKLEEAIISNTIQLA
KRQNTFNKTQFNNLYTGSVGEVRHAILNHSKSAIKG
>Mature_276_residues
MDAEIFSLDSLSIYKDINIASAKPSLKERKNIKHYALDYLSIDEKNNAPLFKTLLEDAMRVSSKEVLLIVGGSSFYLKSI
LEGLSDTPKISGEEAVKIEQEINSLANPYAFLKSIDPTSAFKIHPNDTYRIHKALEIFYSTHTPPSEYFKTNPKKPFEHA
ISLFALSIEKSALANNIKQRTKNMLDCGLIEEIKALYAQYPKDSQPFKAIGVKESILFLEKQLTLKKLEEAIISNTIQLA
KRQNTFNKTQFNNLYTGSVGEVRHAILNHSKSAIKG

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A) [H]

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family [H]

Homologues:

Organism=Homo sapiens, GI31581534, Length=293, Percent_Identity=26.2798634812287, Blast_Score=90, Evalue=3e-18,
Organism=Escherichia coli, GI1790613, Length=250, Percent_Identity=30.8, Blast_Score=117, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI25144712, Length=247, Percent_Identity=27.1255060728745, Blast_Score=71, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6324848, Length=263, Percent_Identity=30.4182509505703, Blast_Score=103, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24649830, Length=264, Percent_Identity=27.6515151515151, Blast_Score=82, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002627
- InterPro:   IPR018022 [H]

Pfam domain/function: PF01715 IPPT [H]

EC number: =2.5.1.75 [H]

Molecular weight: Translated: 31088; Mature: 31088

Theoretical pI: Translated: 9.43; Mature: 9.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDAEIFSLDSLSIYKDINIASAKPSLKERKNIKHYALDYLSIDEKNNAPLFKTLLEDAMR
CCCCEEECCCCHHHHCCCCCCCCCCHHHHHCCHHHHHHHHEECCCCCCHHHHHHHHHHHH
VSSKEVLLIVGGSSFYLKSILEGLSDTPKISGEEAVKIEQEINSLANPYAFLKSIDPTSA
CCCCCEEEEECCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHCCCCCCE
FKIHPNDTYRIHKALEIFYSTHTPPSEYFKTNPKKPFEHAISLFALSIEKSALANNIKQR
EEECCCCCHHHHHHHHHHHHCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
TKNMLDCGLIEEIKALYAQYPKDSQPFKAIGVKESILFLEKQLTLKKLEEAIISNTIQLA
HHHHHCCHHHHHHHHHHHHCCCCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KRQNTFNKTQFNNLYTGSVGEVRHAILNHSKSAIKG
HHHCCCCHHHCCCEECCCHHHHHHHHHHCCHHHCCC
>Mature Secondary Structure
MDAEIFSLDSLSIYKDINIASAKPSLKERKNIKHYALDYLSIDEKNNAPLFKTLLEDAMR
CCCCEEECCCCHHHHCCCCCCCCCCHHHHHCCHHHHHHHHEECCCCCCHHHHHHHHHHHH
VSSKEVLLIVGGSSFYLKSILEGLSDTPKISGEEAVKIEQEINSLANPYAFLKSIDPTSA
CCCCCEEEEECCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHCCCCCCE
FKIHPNDTYRIHKALEIFYSTHTPPSEYFKTNPKKPFEHAISLFALSIEKSALANNIKQR
EEECCCCCHHHHHHHHHHHHCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
TKNMLDCGLIEEIKALYAQYPKDSQPFKAIGVKESILFLEKQLTLKKLEEAIISNTIQLA
HHHHHCCHHHHHHHHHHHHCCCCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KRQNTFNKTQFNNLYTGSVGEVRHAILNHSKSAIKG
HHHCCCCHHHCCCEECCCHHHHHHHHHHCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA