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The map label for this gene is kpnIM [H]

Identifier: 188528159

GI number: 188528159

Start: 1376917

End: 1377993

Strand: Direct

Name: kpnIM [H]

Synonym: HPSH_07090

Alternate gene names: 188528159

Gene position: 1376917-1377993 (Clockwise)

Preceding gene: 188528158

Following gene: 188528160

Centisome position: 85.6

GC content: 36.68

Gene sequence:

>1077_bases
GTGGGGGGGGGGGGGGGGGTGGATAACTTTGTGGCGAGTTTGATATGGCAAAAGAAAAAAGGCGGTAGTCAAGATAGTGA
AAATTTTGCAAAAGAGCATGAGTATATCTTGTGCTATCAAAAAGAAAAATTTAATATCATTGATACAGAAATAGACCATG
ATATACAAGACTTCAATAAAACTATCAATGGCAAACAAGCTAAAATTCTAAAACTTGAAAAATGGGGTAATCATTCTTTG
CGAACAGATAGACCGACCTTATACTATGCGATTAAAGACCCAAATGGTAACGATTTTTATCCTATAGCCCCTAATGGTGA
AGAGGGGTGTTGGCGAAAAAAACCTGAAAATTTAGATAACGAACATATTTTTTGGCAAGAAAATTCTAAAGGGCGTTTAA
TCCCTTATGAAGTAATTTATTATGATGAAATCAAAAATGCGAAAAAAGTGATTAAAACACGCACAATTTTTACAGAGTAT
GGCACTACAACAGAAGCAACTAAAGAAATTTTAGCCCTTTTTAATGGAACTAAACTTTTTGACACCCCCAAACCTGAAGC
CCTACTCAAACGCATTATAGAAATCTCTACCCAAGAAAACGATCTCGTGTTAGACTTTTTTGCCGGGAGCGGGACGACTT
GCGCGGTGGCGCACAAGCTTAAGAGAAAGTATATCGGTATTGAAATGGGGGAGCATTTTGACAGCGTGATTTTGCCTCGC
CTCAAAAAGGTTATAGGCGGTTTTAAAAGCGGTGTGATTAAAGAATTTAATGGAGGTGGGGTGGTGAAAGTTTATGCATT
GGAAAGCTATGAAGAGATTTTAAGAAAAATCAAGCATGAAGACAATGACAAACCCTTAGCTTATGATGAACAATACAGCG
ATTTAGTGGAATGCAAAAACGAATCTTACACGCTCAATTTAAACGCGCTAGAAAAAATGGGCGTGGATATTAAAGAGACT
TTGGAAAATTTATGGGGACTGAAAGTGGAGTTTTTTAATGAAAAGGTGGTGAAATTTAAAGGAAATGATAAAGAAGTAGA
GATTTTAAAAGCCTTAAAAGAAGCGCTCATTTGGTAA

Upstream 100 bases:

>100_bases
AGCTTGCGCACAAGCTTTTGAACGATAAAGGCGTGATGTTTGTGAGCATTGATGACAACGAACAGGCTTATTTAAAAGCG
CTCATGGACGAAGTCTTTAA

Downstream 100 bases:

>100_bases
GGAGGCATAATGGCAAAGAAAAAAGATTTAACAACCCATAATGAAATTTTTGTCGCGCAAAAACTCGCTGAAGATGAATT
GAACACTAACGAGATTAACG

Product: adenine-specific DNA methylase

Products: NA

Alternate protein names: M.KpnI; Adenine-specific methyltransferase KpnI [H]

Number of amino acids: Translated: 358; Mature: 357

Protein sequence:

>358_residues
MGGGGGVDNFVASLIWQKKKGGSQDSENFAKEHEYILCYQKEKFNIIDTEIDHDIQDFNKTINGKQAKILKLEKWGNHSL
RTDRPTLYYAIKDPNGNDFYPIAPNGEEGCWRKKPENLDNEHIFWQENSKGRLIPYEVIYYDEIKNAKKVIKTRTIFTEY
GTTTEATKEILALFNGTKLFDTPKPEALLKRIIEISTQENDLVLDFFAGSGTTCAVAHKLKRKYIGIEMGEHFDSVILPR
LKKVIGGFKSGVIKEFNGGGVVKVYALESYEEILRKIKHEDNDKPLAYDEQYSDLVECKNESYTLNLNALEKMGVDIKET
LENLWGLKVEFFNEKVVKFKGNDKEVEILKALKEALIW

Sequences:

>Translated_358_residues
MGGGGGVDNFVASLIWQKKKGGSQDSENFAKEHEYILCYQKEKFNIIDTEIDHDIQDFNKTINGKQAKILKLEKWGNHSL
RTDRPTLYYAIKDPNGNDFYPIAPNGEEGCWRKKPENLDNEHIFWQENSKGRLIPYEVIYYDEIKNAKKVIKTRTIFTEY
GTTTEATKEILALFNGTKLFDTPKPEALLKRIIEISTQENDLVLDFFAGSGTTCAVAHKLKRKYIGIEMGEHFDSVILPR
LKKVIGGFKSGVIKEFNGGGVVKVYALESYEEILRKIKHEDNDKPLAYDEQYSDLVECKNESYTLNLNALEKMGVDIKET
LENLWGLKVEFFNEKVVKFKGNDKEVEILKALKEALIW
>Mature_357_residues
GGGGGVDNFVASLIWQKKKGGSQDSENFAKEHEYILCYQKEKFNIIDTEIDHDIQDFNKTINGKQAKILKLEKWGNHSLR
TDRPTLYYAIKDPNGNDFYPIAPNGEEGCWRKKPENLDNEHIFWQENSKGRLIPYEVIYYDEIKNAKKVIKTRTIFTEYG
TTTEATKEILALFNGTKLFDTPKPEALLKRIIEISTQENDLVLDFFAGSGTTCAVAHKLKRKYIGIEMGEHFDSVILPRL
KKVIGGFKSGVIKEFNGGGVVKVYALESYEEILRKIKHEDNDKPLAYDEQYSDLVECKNESYTLNLNALEKMGVDIKETL
ENLWGLKVEFFNEKVVKFKGNDKEVEILKALKEALIW

Specific function: This methylase recognizes the double-stranded sequence GGTACC, causes specific methylation on A-4 on both strands, and protects the DNA from cleavage by the KpnI endonuclease [H]

COG id: COG2189

COG function: function code L; Adenine specific DNA methylase Mod

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002295
- InterPro:   IPR002941
- InterPro:   IPR002052 [H]

Pfam domain/function: PF01555 N6_N4_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 41069; Mature: 40938

Theoretical pI: Translated: 6.21; Mature: 6.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGGGGGVDNFVASLIWQKKKGGSQDSENFAKEHEYILCYQKEKFNIIDTEIDHDIQDFNK
CCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHCCCEEEEEECCCCCEECCHHCCCHHHHHH
TINGKQAKILKLEKWGNHSLRTDRPTLYYAIKDPNGNDFYPIAPNGEEGCWRKKPENLDN
HCCCCCEEEEEEECCCCCCCCCCCCEEEEEEECCCCCCEEEECCCCCCCCCCCCCCCCCC
EHIFWQENSKGRLIPYEVIYYDEIKNAKKVIKTRTIFTEYGTTTEATKEILALFNGTKLF
CEEEEEECCCCCEEEEEEEEEHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHCCCCCC
DTPKPEALLKRIIEISTQENDLVLDFFAGSGTTCAVAHKLKRKYIGIEMGEHFDSVILPR
CCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHH
LKKVIGGFKSGVIKEFNGGGVVKVYALESYEEILRKIKHEDNDKPLAYDEQYSDLVECKN
HHHHHHHHHCCCEEEECCCCEEEEEEHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCC
ESYTLNLNALEKMGVDIKETLENLWGLKVEFFNEKVVKFKGNDKEVEILKALKEALIW
CCEEEEEHHHHHHCCCHHHHHHHHHCCEEEEECCEEEEECCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
GGGGGVDNFVASLIWQKKKGGSQDSENFAKEHEYILCYQKEKFNIIDTEIDHDIQDFNK
CCCCCHHHHHHHHHHHHCCCCCCCHHHHHHCCCEEEEEECCCCCEECCHHCCCHHHHHH
TINGKQAKILKLEKWGNHSLRTDRPTLYYAIKDPNGNDFYPIAPNGEEGCWRKKPENLDN
HCCCCCEEEEEEECCCCCCCCCCCCEEEEEEECCCCCCEEEECCCCCCCCCCCCCCCCCC
EHIFWQENSKGRLIPYEVIYYDEIKNAKKVIKTRTIFTEYGTTTEATKEILALFNGTKLF
CEEEEEECCCCCEEEEEEEEEHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHCCCCCC
DTPKPEALLKRIIEISTQENDLVLDFFAGSGTTCAVAHKLKRKYIGIEMGEHFDSVILPR
CCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHH
LKKVIGGFKSGVIKEFNGGGVVKVYALESYEEILRKIKHEDNDKPLAYDEQYSDLVECKN
HHHHHHHHHCCCEEEECCCCEEEEEEHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCC
ESYTLNLNALEKMGVDIKETLENLWGLKVEFFNEKVVKFKGNDKEVEILKALKEALIW
CCEEEEEHHHHHHCCCHHHHHHHHHCCEEEEECCEEEEECCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1754388 [H]