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The map label for this gene is rnhB

Identifier: 188528111

GI number: 188528111

Start: 1329895

End: 1330509

Strand: Reverse

Name: rnhB

Synonym: HPSH_06840

Alternate gene names: 188528111

Gene position: 1330509-1329895 (Counterclockwise)

Preceding gene: 188528112

Following gene: 188528108

Centisome position: 82.71

GC content: 42.93

Gene sequence:

>615_bases
ATGATTTTAGGCATTGATGAAGCGGGGAGAGGGTGTTTGGCCGGGTCGCTTTTTGTGGCTGGGGTGGTGTGTGGTGAAAA
AACAGCCTTAGAGTTTTTAGAAATGGGTCTTAAGGATAGCAAGAAGCTCGGCCCAAAAAAACGCTTTTTCTTGGAAGATA
AAATCAAAACGCATGGCGAGATCAAGTTTTGGGTGGTTAAAAAAAGCGCGAATGAAATTGATAACTTGGGTTTAGGGGCG
TGTTTGAAACTCGCTGTGCAAGAAATTTTAGAAAATGGCCGCTCTTTAGCCAATCAAATAAAAATAGACGGCAACACGGC
GTTTGGCTTGAATAAACGCTACCCTAATATACAAACCATCATCAAGGGCGATGAAAGGATCGCTCAAATCGCTATGGCGT
CTGTTTTAGCGAAAGCCTTTAAAGACAGAGAAATGCGGCAATTGCACGCTTTGTTTAAGGAATACGGCTGGGATAAGAAT
TGCGGGTATGGGACTAAACAGCATATAGAAGCGATGATTAAGCTAGGGGCTACGCCTTTTCATCGGCATAGCTTCACGCT
TAAAAACCGCATCTTAAATCCCAAACTCTTAGATGTGGAACAACGCCTTATTTAA

Upstream 100 bases:

>100_bases
TGAAAAAGAATTAACAGAGTTTATTAAGAAGCTAAGAGAAAACGAGATAACGCATGAAGCGCATGAGTAGGAATTTTTAT
ACCGGTTTGGTTGCGTATCA

Downstream 100 bases:

>100_bases
AATGGCACTGAGATTGGTAGCGCTCGTTGGAGAAAGGTTGATATGCTTTGCTTTAAAGTAAGCGATAAGATCTTCAGGCT
GAATGAAAGGGAATTGCACC

Product: ribonuclease HII

Products: NA

Alternate protein names: RNase HII

Number of amino acids: Translated: 204; Mature: 204

Protein sequence:

>204_residues
MILGIDEAGRGCLAGSLFVAGVVCGEKTALEFLEMGLKDSKKLGPKKRFFLEDKIKTHGEIKFWVVKKSANEIDNLGLGA
CLKLAVQEILENGRSLANQIKIDGNTAFGLNKRYPNIQTIIKGDERIAQIAMASVLAKAFKDREMRQLHALFKEYGWDKN
CGYGTKQHIEAMIKLGATPFHRHSFTLKNRILNPKLLDVEQRLI

Sequences:

>Translated_204_residues
MILGIDEAGRGCLAGSLFVAGVVCGEKTALEFLEMGLKDSKKLGPKKRFFLEDKIKTHGEIKFWVVKKSANEIDNLGLGA
CLKLAVQEILENGRSLANQIKIDGNTAFGLNKRYPNIQTIIKGDERIAQIAMASVLAKAFKDREMRQLHALFKEYGWDKN
CGYGTKQHIEAMIKLGATPFHRHSFTLKNRILNPKLLDVEQRLI
>Mature_204_residues
MILGIDEAGRGCLAGSLFVAGVVCGEKTALEFLEMGLKDSKKLGPKKRFFLEDKIKTHGEIKFWVVKKSANEIDNLGLGA
CLKLAVQEILENGRSLANQIKIDGNTAFGLNKRYPNIQTIIKGDERIAQIAMASVLAKAFKDREMRQLHALFKEYGWDKN
CGYGTKQHIEAMIKLGATPFHRHSFTLKNRILNPKLLDVEQRLI

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0164

COG function: function code L; Ribonuclease HII

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase HII family

Homologues:

Organism=Escherichia coli, GI1786380, Length=192, Percent_Identity=33.8541666666667, Blast_Score=106, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH2_HELPS (B2UV86)

Other databases:

- EMBL:   CP001072
- RefSeq:   YP_001910798.1
- GeneID:   6296877
- GenomeReviews:   CP001072_GR
- KEGG:   hps:HPSH_06840
- HOGENOM:   HBG584843
- OMA:   RLGPTPI
- ProtClustDB:   PRK14550
- GO:   GO:0005737
- HAMAP:   MF_00052_B
- InterPro:   IPR022898
- InterPro:   IPR001352
- InterPro:   IPR012337
- PANTHER:   PTHR10954

Pfam domain/function: PF01351 RNase_HII; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 22841; Mature: 22841

Theoretical pI: Translated: 10.07; Mature: 10.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILGIDEAGRGCLAGSLFVAGVVCGEKTALEFLEMGLKDSKKLGPKKRFFLEDKIKTHGE
CEECCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHCCCCHHHHHHHHHCCCCC
IKFWVVKKSANEIDNLGLGACLKLAVQEILENGRSLANQIKIDGNTAFGLNKRYPNIQTI
EEEEEEECCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHEEECCCEEECCCCCCCCHHHE
IKGDERIAQIAMASVLAKAFKDREMRQLHALFKEYGWDKNCGYGTKQHIEAMIKLGATPF
ECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCC
HRHSFTLKNRILNPKLLDVEQRLI
HHHHHHHHHHCCCCHHCCHHHHCC
>Mature Secondary Structure
MILGIDEAGRGCLAGSLFVAGVVCGEKTALEFLEMGLKDSKKLGPKKRFFLEDKIKTHGE
CEECCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHCCCCHHHHHHHHHCCCCC
IKFWVVKKSANEIDNLGLGACLKLAVQEILENGRSLANQIKIDGNTAFGLNKRYPNIQTI
EEEEEEECCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHEEECCCEEECCCCCCCCHHHE
IKGDERIAQIAMASVLAKAFKDREMRQLHALFKEYGWDKNCGYGTKQHIEAMIKLGATPF
ECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCC
HRHSFTLKNRILNPKLLDVEQRLI
HHHHHHHHHHCCCCHHCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA