Click here to switch to the map view.

The map label for this gene is amiF

Identifier: 188528026

GI number: 188528026

Start: 1261913

End: 1262917

Strand: Direct

Name: amiF

Synonym: HPSH_06405

Alternate gene names: 188528026

Gene position: 1261913-1262917 (Clockwise)

Preceding gene: 188528025

Following gene: 188528030

Centisome position: 78.45

GC content: 44.78

Gene sequence:

>1005_bases
ATGGGAAGTATCGGTAGTATGGGCAAACCTATTGAAGGGTTTTTAGTGGCAGCCATTCAGTTTCCTGTGCCAATTGTCAA
TAGCCGTAAGGATATTGATCACAATATTGAAAGCATTATTAGAACCTTGCATGCGACTAAAGCGGGGTATCCGGGAGTGG
AACTTATCATTTTCCCTGAATATAGCACGCAAGGTTTGAATACCGCTAAGTGGCTCAGCGAAGAGTTTTTATTAGATGTC
CCGGGTAAAGAAACAGAACTATACGCTAAGGCGTGTAAAGAGGCGAAAGTTTATGGTGTTTTTTCAATCATGGAACGCAA
TCCTGATTCTAACAAAAACCCCTACAACACCGCCATCATCATTGATCCGCAAGGTAAAATCATTTTAAAATACCGCAAAC
TATTCCCATGGAATCCCATTGAGCCATGGTATCCTGGGGATTTAGGAATGCCTGTGTGCGAGGGTCCGGGCGGATCAAAA
TTAGCCGTGTGCATTTGCCATGACGGCATGATTCCAGAGCTCGCTAGAGAAGCGGCCTATAAAGGGTGCAATGTGTATAT
CCGCATTTCAGGCTATAGCACTCAAGTCAATGATCAATGGATTTTGACCAACCGCTCCAATGCGTGGCACAATTTGATGT
ATACCGTGAGCGTGAATTTAGCCGGCTATGACAATGTCTTTTATTACTTTGGCGAGGGGCAAATCTGTAACTTTGATGGC
ACGACTCTTGTTCAAGGGCACCGCAACCCTTGGGAGATTGTAACTGGGGAAATCTATCCTAAAATGGCTGACAACGCTCG
CTTAAGCTGGGGACTAGAAAACAATATTTACAACCTAGGCCATAGAGGGTATGTGGCTAAACCGGGCGGAGAACATGACG
CAGGCTTAACCTACATCAAAGACTTAGCCGCTGGTAAATACAAATTGCCTTGGGAAGATCACATGAAAATCAAAGATGGC
TCTGTTTATGGCTACCCTACCACCGGTGGGCGTTTTGGGAAATAA

Upstream 100 bases:

>100_bases
CAAAAAATCTCGCATTTACTCTAAATTAGTTCTCTTGCAATAGTATTTTATCGCAATAACTATTATTGTTATTGCGATAA
AACTTTTAGAAGGAGTTATT

Downstream 100 bases:

>100_bases
TCCCTAACCTTGTATTTTGCTAGAACCTGTTTAAAGGTTCTAGTTCCCTTATTTTTAAACAATGAGGGAATAATAAGACT
TATTCATATTGGATAAAGAC

Product: formamidase

Products: NA

Alternate protein names: Formamide amidohydrolase [H]

Number of amino acids: Translated: 334; Mature: 333

Protein sequence:

>334_residues
MGSIGSMGKPIEGFLVAAIQFPVPIVNSRKDIDHNIESIIRTLHATKAGYPGVELIIFPEYSTQGLNTAKWLSEEFLLDV
PGKETELYAKACKEAKVYGVFSIMERNPDSNKNPYNTAIIIDPQGKIILKYRKLFPWNPIEPWYPGDLGMPVCEGPGGSK
LAVCICHDGMIPELAREAAYKGCNVYIRISGYSTQVNDQWILTNRSNAWHNLMYTVSVNLAGYDNVFYYFGEGQICNFDG
TTLVQGHRNPWEIVTGEIYPKMADNARLSWGLENNIYNLGHRGYVAKPGGEHDAGLTYIKDLAAGKYKLPWEDHMKIKDG
SVYGYPTTGGRFGK

Sequences:

>Translated_334_residues
MGSIGSMGKPIEGFLVAAIQFPVPIVNSRKDIDHNIESIIRTLHATKAGYPGVELIIFPEYSTQGLNTAKWLSEEFLLDV
PGKETELYAKACKEAKVYGVFSIMERNPDSNKNPYNTAIIIDPQGKIILKYRKLFPWNPIEPWYPGDLGMPVCEGPGGSK
LAVCICHDGMIPELAREAAYKGCNVYIRISGYSTQVNDQWILTNRSNAWHNLMYTVSVNLAGYDNVFYYFGEGQICNFDG
TTLVQGHRNPWEIVTGEIYPKMADNARLSWGLENNIYNLGHRGYVAKPGGEHDAGLTYIKDLAAGKYKLPWEDHMKIKDG
SVYGYPTTGGRFGK
>Mature_333_residues
GSIGSMGKPIEGFLVAAIQFPVPIVNSRKDIDHNIESIIRTLHATKAGYPGVELIIFPEYSTQGLNTAKWLSEEFLLDVP
GKETELYAKACKEAKVYGVFSIMERNPDSNKNPYNTAIIIDPQGKIILKYRKLFPWNPIEPWYPGDLGMPVCEGPGGSKL
AVCICHDGMIPELAREAAYKGCNVYIRISGYSTQVNDQWILTNRSNAWHNLMYTVSVNLAGYDNVFYYFGEGQICNFDGT
TLVQGHRNPWEIVTGEIYPKMADNARLSWGLENNIYNLGHRGYVAKPGGEHDAGLTYIKDLAAGKYKLPWEDHMKIKDGS
VYGYPTTGGRFGK

Specific function: Is an aliphatic amidase with a restricted substrate specificity, as it only hydrolyzes formamide [H]

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022843
- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: =3.5.1.49 [H]

Molecular weight: Translated: 37276; Mature: 37145

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGSIGSMGKPIEGFLVAAIQFPVPIVNSRKDIDHNIESIIRTLHATKAGYPGVELIIFPE
CCCCCCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEEC
YSTQGLNTAKWLSEEFLLDVPGKETELYAKACKEAKVYGVFSIMERNPDSNKNPYNTAII
CCCCCCCHHHHHCCCEEEECCCCHHHHHHHHHHCCEEEEEEEEEECCCCCCCCCCEEEEE
IDPQGKIILKYRKLFPWNPIEPWYPGDLGMPVCEGPGGSKLAVCICHDGMIPELAREAAY
ECCCCCEEEEEEECCCCCCCCCCCCCCCCCCEECCCCCCEEEEEEECCCCCHHHHHHHHC
KGCNVYIRISGYSTQVNDQWILTNRSNAWHNLMYTVSVNLAGYDNVFYYFGEGQICNFDG
CCCEEEEEEECCEEECCCEEEEECCCCCCEEEEEEEEEEEECCCCEEEEECCCEEECCCC
TTLVQGHRNPWEIVTGEIYPKMADNARLSWGLENNIYNLGHRGYVAKPGGEHDAGLTYIK
CEEEECCCCCCEEEECEECCCCCCCCEEEECCCCCEEECCCCCEEECCCCCCCCCHHHHH
DLAAGKYKLPWEDHMKIKDGSVYGYPTTGGRFGK
HHCCCCEECCHHHCCEEECCEEEECCCCCCCCCC
>Mature Secondary Structure 
GSIGSMGKPIEGFLVAAIQFPVPIVNSRKDIDHNIESIIRTLHATKAGYPGVELIIFPE
CCCCCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEEC
YSTQGLNTAKWLSEEFLLDVPGKETELYAKACKEAKVYGVFSIMERNPDSNKNPYNTAII
CCCCCCCHHHHHCCCEEEECCCCHHHHHHHHHHCCEEEEEEEEEECCCCCCCCCCEEEEE
IDPQGKIILKYRKLFPWNPIEPWYPGDLGMPVCEGPGGSKLAVCICHDGMIPELAREAAY
ECCCCCEEEEEEECCCCCCCCCCCCCCCCCCEECCCCCCEEEEEEECCCCCHHHHHHHHC
KGCNVYIRISGYSTQVNDQWILTNRSNAWHNLMYTVSVNLAGYDNVFYYFGEGQICNFDG
CCCEEEEEEECCEEECCCEEEEECCCCCCEEEEEEEEEEEECCCCEEEEECCCEEECCCC
TTLVQGHRNPWEIVTGEIYPKMADNARLSWGLENNIYNLGHRGYVAKPGGEHDAGLTYIK
CEEEECCCCCCEEEECEECCCCCCCCEEEECCCCCEEECCCCCEEECCCCCCCCCHHHHH
DLAAGKYKLPWEDHMKIKDGSVYGYPTTGGRFGK
HHCCCCEECCHHHCCEEECCEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA