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The map label for this gene is rfaF [H]

Identifier: 188527978

GI number: 188527978

Start: 1213123

End: 1214172

Strand: Direct

Name: rfaF [H]

Synonym: HPSH_06165

Alternate gene names: 188527978

Gene position: 1213123-1214172 (Clockwise)

Preceding gene: 188527975

Following gene: 188527980

Centisome position: 75.42

GC content: 40.29

Gene sequence:

>1050_bases
ATGAGCGTAAATGCGCCCAAACGCATGCGTATTTTATTGCGTTTGCCTAATTGGTTAGGCGATGGGGTGATGGCAAGCTC
GCTTTTTTACACCCTTAAACACCACTACCCTAACGCGCGTTTCATCTTAGTGGGCCCACAAATGACTTGCGAACTCTTCA
AAAAAGATGAAAAAATAGAAGCCGTTTTTATAGATGACACCAAAAAATCCTTTTTCAGGCTGCTAGCCACTCACAAACTC
GCTCAAAAAATAGGGCGTTGCGATATAGCGATCACTTTAAACAACCATTTTTATTCCGCTTTTTTGCTCTATGCGACAAA
AACGCCTATTCGCATCGGTTTTGCTCAATTTTTTCGTTCTTTGTTCCTTAGCCATGCGGTAATTTCTGCCCCTAAAGAGT
ATCATCAAGTGGAAAAGTATTGCTTTTTATTTTCGCAATTTTTGAAAAAAGAATTGGATAAAAAAAGCGTTTTGCCCTTA
AAACTGGCCTTTAACCTCCCCACTCACACCCCAAACACCCCTAAAAAAATCGGCTTTAACCCTAGCGCAAGCTATGGGAG
CGCTAAAAGATGGCCAGCTTCTTATTACGCTGAAGTTTCTGCTGCTTTGTTAGAAGAAGGGCATGAAATTTATTTTTTTG
GGGCTAAAGAAGACGCTATCGTTTCTGAAGAGATTTTAAAACTCATCAAAGGTTTATTAAAAAACCCTTTATTATTCAAT
AACGCTTACAATCTGTGCGGGAAAACAAGCATTGAAGAATTGATAGAGCACATCGCTATTTTGGATTTATTCATCACTAA
CGATAGCGGTCCTATGCATGTGGCTGCCAGCACACAAACCCCCTTAATCGCTCTTTTTGGTCCCACTGATGAAAGAGAAA
CTCGCCCTTATAAAGCTCAAAAAACGATCGTGTTGAACCACCACCTAAGCTGTTCGCCATGCAAGAAACGAGTTTGCCCT
TTAAAGAATGAAAAAAACCATTTGTGCATGAAATCTATCACGCCCCTTGAAGTCTTAAAAGCCGCTCACACTCTTTTAGA
AAAGCCTTAA

Upstream 100 bases:

>100_bases
GCGGTCTTTAAACCCGCTCAACACTTTAGGGGTGATCATTCCATTTCCTTGTATTTGTATGCTAAAATTTTAGCTTAAAA
TCTTTAAAAAAGGGCTATTG

Downstream 100 bases:

>100_bases
ACGCCTTTTTATGCACTTTTTAAAAACACAGCCGTATTGACAAAATTCTAGTATATAAAAGATTCAAACTTTACTTTTAA
AGGCACAAGAAGAGCGATAA

Product: ADP-heptose-lps heptosyltransferase II

Products: NA

Alternate protein names: ADP-heptose--LPS heptosyltransferase II [H]

Number of amino acids: Translated: 349; Mature: 348

Protein sequence:

>349_residues
MSVNAPKRMRILLRLPNWLGDGVMASSLFYTLKHHYPNARFILVGPQMTCELFKKDEKIEAVFIDDTKKSFFRLLATHKL
AQKIGRCDIAITLNNHFYSAFLLYATKTPIRIGFAQFFRSLFLSHAVISAPKEYHQVEKYCFLFSQFLKKELDKKSVLPL
KLAFNLPTHTPNTPKKIGFNPSASYGSAKRWPASYYAEVSAALLEEGHEIYFFGAKEDAIVSEEILKLIKGLLKNPLLFN
NAYNLCGKTSIEELIEHIAILDLFITNDSGPMHVAASTQTPLIALFGPTDERETRPYKAQKTIVLNHHLSCSPCKKRVCP
LKNEKNHLCMKSITPLEVLKAAHTLLEKP

Sequences:

>Translated_349_residues
MSVNAPKRMRILLRLPNWLGDGVMASSLFYTLKHHYPNARFILVGPQMTCELFKKDEKIEAVFIDDTKKSFFRLLATHKL
AQKIGRCDIAITLNNHFYSAFLLYATKTPIRIGFAQFFRSLFLSHAVISAPKEYHQVEKYCFLFSQFLKKELDKKSVLPL
KLAFNLPTHTPNTPKKIGFNPSASYGSAKRWPASYYAEVSAALLEEGHEIYFFGAKEDAIVSEEILKLIKGLLKNPLLFN
NAYNLCGKTSIEELIEHIAILDLFITNDSGPMHVAASTQTPLIALFGPTDERETRPYKAQKTIVLNHHLSCSPCKKRVCP
LKNEKNHLCMKSITPLEVLKAAHTLLEKP
>Mature_348_residues
SVNAPKRMRILLRLPNWLGDGVMASSLFYTLKHHYPNARFILVGPQMTCELFKKDEKIEAVFIDDTKKSFFRLLATHKLA
QKIGRCDIAITLNNHFYSAFLLYATKTPIRIGFAQFFRSLFLSHAVISAPKEYHQVEKYCFLFSQFLKKELDKKSVLPLK
LAFNLPTHTPNTPKKIGFNPSASYGSAKRWPASYYAEVSAALLEEGHEIYFFGAKEDAIVSEEILKLIKGLLKNPLLFNN
AYNLCGKTSIEELIEHIAILDLFITNDSGPMHVAASTQTPLIALFGPTDERETRPYKAQKTIVLNHHLSCSPCKKRVCPL
KNEKNHLCMKSITPLEVLKAAHTLLEKP

Specific function: Lipopolysaccharide core biosynthesis. [C]

COG id: COG0859

COG function: function code M; ADP-heptose:LPS heptosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 9 family [H]

Homologues:

Organism=Escherichia coli, GI1790050, Length=360, Percent_Identity=29.7222222222222, Blast_Score=125, Evalue=3e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002201
- InterPro:   IPR011910 [H]

Pfam domain/function: PF01075 Glyco_transf_9 [H]

EC number: 2.4.1.-

Molecular weight: Translated: 39599; Mature: 39468

Theoretical pI: Translated: 9.75; Mature: 9.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVNAPKRMRILLRLPNWLGDGVMASSLFYTLKHHYPNARFILVGPQMTCELFKKDEKIE
CCCCCCHHHEEEEECCCHHCCHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHCCCCCEE
AVFIDDTKKSFFRLLATHKLAQKIGRCDIAITLNNHFYSAFLLYATKTPIRIGFAQFFRS
EEEEECHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEEEEEEEEECCCHHHHHHHHHHH
LFLSHAVISAPKEYHQVEKYCFLFSQFLKKELDKKSVLPLKLAFNLPTHTPNTPKKIGFN
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCCC
PSASYGSAKRWPASYYAEVSAALLEEGHEIYFFGAKEDAIVSEEILKLIKGLLKNPLLFN
CCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCEEEC
NAYNLCGKTSIEELIEHIAILDLFITNDSGPMHVAASTQTPLIALFGPTDERETRPYKAQ
CCHHHCCCCCHHHHHHHHHHHEEEEECCCCCEEEEECCCCCEEEEECCCCCCCCCCCCCC
KTIVLNHHLSCSPCKKRVCPLKNEKNHLCMKSITPLEVLKAAHTLLEKP
EEEEEECCCCCCHHHHCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SVNAPKRMRILLRLPNWLGDGVMASSLFYTLKHHYPNARFILVGPQMTCELFKKDEKIE
CCCCCHHHEEEEECCCHHCCHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHCCCCCEE
AVFIDDTKKSFFRLLATHKLAQKIGRCDIAITLNNHFYSAFLLYATKTPIRIGFAQFFRS
EEEEECHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEEEEEEEEECCCHHHHHHHHHHH
LFLSHAVISAPKEYHQVEKYCFLFSQFLKKELDKKSVLPLKLAFNLPTHTPNTPKKIGFN
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCCC
PSASYGSAKRWPASYYAEVSAALLEEGHEIYFFGAKEDAIVSEEILKLIKGLLKNPLLFN
CCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCEEEC
NAYNLCGKTSIEELIEHIAILDLFITNDSGPMHVAASTQTPLIALFGPTDERETRPYKAQ
CCHHHCCCCCHHHHHHHHHHHEEEEECCCCCEEEEECCCCCEEEEECCCCCCCCCCCCCC
KTIVLNHHLSCSPCKKRVCPLKNEKNHLCMKSITPLEVLKAAHTLLEKP
EEEEEECCCCCCHHHHCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]