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The map label for this gene is priA [H]

Identifier: 188527848

GI number: 188527848

Start: 1081074

End: 1082933

Strand: Reverse

Name: priA [H]

Synonym: HPSH_05470

Alternate gene names: 188527848

Gene position: 1082933-1081074 (Counterclockwise)

Preceding gene: 188527849

Following gene: 188527845

Centisome position: 67.32

GC content: 38.01

Gene sequence:

>1860_bases
ATGTTCTATCACTTGATCGCTCCTTTAAAAAATAAAACCCCCCCTTTAACTTACTTTTCTAAGGAGCGGCACCAAAAAGG
AGCGTTAGTCAATATCCATTTAAGGAATAAAATGCTTTTGGGCGTCGTTCTTGGAGAAGTTTCAAAACCCTCTTTTGAAT
GCCTAGAACTAGAAAAAACCCCTTATTTTTTACTCCCCTTTCAAATAGAGCTCGCTATATTTATCGCTCAATATTACTCG
GCTAATCTTTCTTCAGTCTTAAATCTTTTTACCCCTTTTAAAGAATGCGATTTGGTGGGGTTAGAAAAAATTGAGCCCGT
TCTTAATGCGTTAAGCCAAACGCAAACAAACGCTTTAAAAGAATTGCAAAAACATTCAGCAAGCCTGCTCTTTGGCGATA
CGGGTAGCGGGAAAACCGAGATTTATATGCATTCAATCGCTCAAACTTTAGAGCAAAAAAAAAGTGCTTTATTGTTAGTG
CCAGAAATCGCCCTCACCCCTCAAATGCAACAACGCCTTAAAAGGGTCTTTAAAGAAAATTTAGGCTTGTGGCATAGCAA
ACTCTCTCAAACCCAAAAAAAACAATTTTTAGAAAAGCTTTATTCGCAAGAAATCAAATTAGTGGTAGGCACACGAAGCG
CGTTGTTTTTACCCCTTAAGGAGTTGGGTTTAATCATTGTAGATGAAGAGCATGACTTTTCTTATAAATCTCATCAAAGC
CCTATGTATAACGCTAGGGATTTATGCTTGTATTTATCCCATAAATTCCCTATTCAAGTGATTTTAGGCTCTGCTACGCC
AAGTTTAAATAGTTACAAACGCTTTAAAGATAAGGCTTTAGTGCGCTTAAAGGGGCGCTACACGCCTACGCAAAAAAACA
TTATTTTTGAAAAAACCGAGCGTTTTATCACGCCCAAACTCCTAGAAGCGCTAAAACAAGTTATAGACAAAAACGAGCAA
GCCATTATTTTTGTGCCTACAAGGGCTAATTTCAAAACCTTGCTGTGTCAAAATTGTTACAAAAGCGTTCAATGCCCCTT
TTGCAGCGTGAATATGAGTTTGCATTTAAAAACCAACAAACTCATGTGCCATTATTGCCATTTTTCAAGCCCTATCCCTA
AAATTTGCAGCGCGTGTCAAAACGAAGTTTTAGTGGGTAAAAGGATAGGCACCATGCAAGTGTTAAACGAGTTGGAAGGC
CTTTTGCAAGGCACTAAAATAGCCATTTTAGATAAAGATCATACCAGCACGCCAAAAAAACTCCACAATATTTTAAACGA
TTTCAACGCTCAAAAAACCAATATCTTAATCGGCACTCAAATGATAAGCAAAGGGCATGATTACGCTAAAGTGAGTTTAG
CGGTTGTTTTAGGCATAGACAATATCATTAAATCCAATAGTTACAGGGCTTTAGAAGAAGGCGTGTCATTACTTTATCAA
ATCGCTGGGAGGAGCGCTAGGCAAATTTCTGGCCAAGTGTTCATTCAAAGCACCGAAACGGATCTATTAGAAAATTTCTT
AGAAGATTATGAAGATTTTTTACAATACGAATTGCAAGAAAGGTGCGAACTCTACCCGCCTTTTTCAAGGCTGTGTTTGT
TAGAGTTTAAGCATAAAAACGAAGAAAAAGCCCAACAATTGAGCCTAAAAGCCTCTCAAACCCTTTCTTCGTGTTTAGAA
AAGGGTGTAACGCTCTCTAGCTTTAAAGCCCCCATTGAAAAAATCGCTTCTTCTTATCGCTACCTTATTTTATTGCGTTC
CAAAAACCCTTTAAGCCTAATCAAAAGCGTGCATGCGTTTTTAAAAACCGCCCCCAATATCCCTTGCAGCGTGAATATGG
ATCCTGTGGATATTTTTTAA

Upstream 100 bases:

>100_bases
AATTAAACGCCTTAGAGCTTTTAAAGGATTATTTGCATGAAAGTTGTCAAAACGAGTATTTGCACAATGAACTCAAAAAG
CTTTTAGAAAAAATCTCATC

Downstream 100 bases:

>100_bases
AAAACTTTATATATTATTTCAAAAAACTCAGGTTTTTCTGGCGACTAGTAGCGTGAAATTCACCCATTTAAACAGCGCTT
CCACATGCTTAAACCCCACG

Product: primosome assembly protein PriA

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 619; Mature: 619

Protein sequence:

>619_residues
MFYHLIAPLKNKTPPLTYFSKERHQKGALVNIHLRNKMLLGVVLGEVSKPSFECLELEKTPYFLLPFQIELAIFIAQYYS
ANLSSVLNLFTPFKECDLVGLEKIEPVLNALSQTQTNALKELQKHSASLLFGDTGSGKTEIYMHSIAQTLEQKKSALLLV
PEIALTPQMQQRLKRVFKENLGLWHSKLSQTQKKQFLEKLYSQEIKLVVGTRSALFLPLKELGLIIVDEEHDFSYKSHQS
PMYNARDLCLYLSHKFPIQVILGSATPSLNSYKRFKDKALVRLKGRYTPTQKNIIFEKTERFITPKLLEALKQVIDKNEQ
AIIFVPTRANFKTLLCQNCYKSVQCPFCSVNMSLHLKTNKLMCHYCHFSSPIPKICSACQNEVLVGKRIGTMQVLNELEG
LLQGTKIAILDKDHTSTPKKLHNILNDFNAQKTNILIGTQMISKGHDYAKVSLAVVLGIDNIIKSNSYRALEEGVSLLYQ
IAGRSARQISGQVFIQSTETDLLENFLEDYEDFLQYELQERCELYPPFSRLCLLEFKHKNEEKAQQLSLKASQTLSSCLE
KGVTLSSFKAPIEKIASSYRYLILLRSKNPLSLIKSVHAFLKTAPNIPCSVNMDPVDIF

Sequences:

>Translated_619_residues
MFYHLIAPLKNKTPPLTYFSKERHQKGALVNIHLRNKMLLGVVLGEVSKPSFECLELEKTPYFLLPFQIELAIFIAQYYS
ANLSSVLNLFTPFKECDLVGLEKIEPVLNALSQTQTNALKELQKHSASLLFGDTGSGKTEIYMHSIAQTLEQKKSALLLV
PEIALTPQMQQRLKRVFKENLGLWHSKLSQTQKKQFLEKLYSQEIKLVVGTRSALFLPLKELGLIIVDEEHDFSYKSHQS
PMYNARDLCLYLSHKFPIQVILGSATPSLNSYKRFKDKALVRLKGRYTPTQKNIIFEKTERFITPKLLEALKQVIDKNEQ
AIIFVPTRANFKTLLCQNCYKSVQCPFCSVNMSLHLKTNKLMCHYCHFSSPIPKICSACQNEVLVGKRIGTMQVLNELEG
LLQGTKIAILDKDHTSTPKKLHNILNDFNAQKTNILIGTQMISKGHDYAKVSLAVVLGIDNIIKSNSYRALEEGVSLLYQ
IAGRSARQISGQVFIQSTETDLLENFLEDYEDFLQYELQERCELYPPFSRLCLLEFKHKNEEKAQQLSLKASQTLSSCLE
KGVTLSSFKAPIEKIASSYRYLILLRSKNPLSLIKSVHAFLKTAPNIPCSVNMDPVDIF
>Mature_619_residues
MFYHLIAPLKNKTPPLTYFSKERHQKGALVNIHLRNKMLLGVVLGEVSKPSFECLELEKTPYFLLPFQIELAIFIAQYYS
ANLSSVLNLFTPFKECDLVGLEKIEPVLNALSQTQTNALKELQKHSASLLFGDTGSGKTEIYMHSIAQTLEQKKSALLLV
PEIALTPQMQQRLKRVFKENLGLWHSKLSQTQKKQFLEKLYSQEIKLVVGTRSALFLPLKELGLIIVDEEHDFSYKSHQS
PMYNARDLCLYLSHKFPIQVILGSATPSLNSYKRFKDKALVRLKGRYTPTQKNIIFEKTERFITPKLLEALKQVIDKNEQ
AIIFVPTRANFKTLLCQNCYKSVQCPFCSVNMSLHLKTNKLMCHYCHFSSPIPKICSACQNEVLVGKRIGTMQVLNELEG
LLQGTKIAILDKDHTSTPKKLHNILNDFNAQKTNILIGTQMISKGHDYAKVSLAVVLGIDNIIKSNSYRALEEGVSLLYQ
IAGRSARQISGQVFIQSTETDLLENFLEDYEDFLQYELQERCELYPPFSRLCLLEFKHKNEEKAQQLSLKASQTLSSCLE
KGVTLSSFKAPIEKIASSYRYLILLRSKNPLSLIKSVHAFLKTAPNIPCSVNMDPVDIF

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=562, Percent_Identity=33.0960854092527, Blast_Score=283, Evalue=3e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 70396; Mature: 70396

Theoretical pI: Translated: 9.33; Mature: 9.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFYHLIAPLKNKTPPLTYFSKERHQKGALVNIHLRNKMLLGVVLGEVSKPSFECLELEKT
CCEEEECCCCCCCCCCCCHHHHHCCCCCEEEEEECCHHEEHHHHHCCCCCCCCEEEECCC
PYFLLPFQIELAIFIAQYYSANLSSVLNLFTPFKECDLVGLEKIEPVLNALSQTQTNALK
CEEEEEHHHHHHHHHHHHHCCCHHHHHHHHCCHHHCCCCCHHHHHHHHHHHHHHHHHHHH
ELQKHSASLLFGDTGSGKTEIYMHSIAQTLEQKKSALLLVPEIALTPQMQQRLKRVFKEN
HHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCEEEEECCHHCCHHHHHHHHHHHHHH
LGLWHSKLSQTQKKQFLEKLYSQEIKLVVGTRSALFLPLKELGLIIVDEEHDFSYKSHQS
HCHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCEEEEHHHCCEEEEECCCCCCCCCCCC
PMYNARDLCLYLSHKFPIQVILGSATPSLNSYKRFKDKALVRLKGRYTPTQKNIIFEKTE
CCCCHHHEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHEEEEECCCCCCCCCEEHHHHH
RFITPKLLEALKQVIDKNEQAIIFVPTRANFKTLLCQNCYKSVQCPFCSVNMSLHLKTNK
HHCCHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCEEEECEEEEEECCC
LMCHYCHFSSPIPKICSACQNEVLVGKRIGTMQVLNELEGLLQGTKIAILDKDHTSTPKK
EEEEEECCCCCHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCHHH
LHNILNDFNAQKTNILIGTQMISKGHDYAKVSLAVVLGIDNIIKSNSYRALEEGVSLLYQ
HHHHHHHCCCCCCEEEEEHHHHHCCCCHHHEEEEEEECHHHHHHCCCHHHHHHHHHHHHH
IAGRSARQISGQVFIQSTETDLLENFLEDYEDFLQYELQERCELYPPFSRLCLLEFKHKN
HHCCHHHCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCC
EEKAQQLSLKASQTLSSCLEKGVTLSSFKAPIEKIASSYRYLILLRSKNPLSLIKSVHAF
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHH
LKTAPNIPCSVNMDPVDIF
HHCCCCCCEECCCCCCCCC
>Mature Secondary Structure
MFYHLIAPLKNKTPPLTYFSKERHQKGALVNIHLRNKMLLGVVLGEVSKPSFECLELEKT
CCEEEECCCCCCCCCCCCHHHHHCCCCCEEEEEECCHHEEHHHHHCCCCCCCCEEEECCC
PYFLLPFQIELAIFIAQYYSANLSSVLNLFTPFKECDLVGLEKIEPVLNALSQTQTNALK
CEEEEEHHHHHHHHHHHHHCCCHHHHHHHHCCHHHCCCCCHHHHHHHHHHHHHHHHHHHH
ELQKHSASLLFGDTGSGKTEIYMHSIAQTLEQKKSALLLVPEIALTPQMQQRLKRVFKEN
HHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCEEEEECCHHCCHHHHHHHHHHHHHH
LGLWHSKLSQTQKKQFLEKLYSQEIKLVVGTRSALFLPLKELGLIIVDEEHDFSYKSHQS
HCHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCEEEEHHHCCEEEEECCCCCCCCCCCC
PMYNARDLCLYLSHKFPIQVILGSATPSLNSYKRFKDKALVRLKGRYTPTQKNIIFEKTE
CCCCHHHEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHEEEEECCCCCCCCCEEHHHHH
RFITPKLLEALKQVIDKNEQAIIFVPTRANFKTLLCQNCYKSVQCPFCSVNMSLHLKTNK
HHCCHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCEEEECEEEEEECCC
LMCHYCHFSSPIPKICSACQNEVLVGKRIGTMQVLNELEGLLQGTKIAILDKDHTSTPKK
EEEEEECCCCCHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCHHH
LHNILNDFNAQKTNILIGTQMISKGHDYAKVSLAVVLGIDNIIKSNSYRALEEGVSLLYQ
HHHHHHHCCCCCCEEEEEHHHHHCCCCHHHEEEEEEECHHHHHHCCCHHHHHHHHHHHHH
IAGRSARQISGQVFIQSTETDLLENFLEDYEDFLQYELQERCELYPPFSRLCLLEFKHKN
HHCCHHHCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCC
EEKAQQLSLKASQTLSSCLEKGVTLSSFKAPIEKIASSYRYLILLRSKNPLSLIKSVHAF
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHH
LKTAPNIPCSVNMDPVDIF
HHCCCCCCEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]