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The map label for this gene is metG [H]

Identifier: 188527820

GI number: 188527820

Start: 1047921

End: 1049909

Strand: Reverse

Name: metG [H]

Synonym: HPSH_05325

Alternate gene names: 188527820

Gene position: 1049909-1047921 (Counterclockwise)

Preceding gene: 188527822

Following gene: 188527819

Centisome position: 65.27

GC content: 38.51

Gene sequence:

>1989_bases
ATGCAAAAATCACTGATCACAACCCCCATTTATTATGTGAATGATGTCCCCCATATTGGCCATGCTTATACGACTTTGAT
TGCGGATACTCTAAAGAAGTATTACACGCTTCAAGGCGAAGAAGTCTTTTTTTTAACCGGCACCGATGAGCATGGGCAAA
AGATCGAACAAAGCGCGAGATTGAGGAATCAAAGCCCTAAAGCTTACGCCGATAGCATTAGCGCGATTTTTAAAGACCAG
TGGGATTTTTTCAATTTAGATTATGATGGTTTTATCCGCACCACAGACAGCGAGCATCAAAAATGCGTGCAAAACGCCTT
TGAAATCATGTTTGAAAAAGGGGATATTTATAAAGGCGCTTATAGCGGGTATTATTGCGTGAGCTGTGAGAGTTATTGCG
CGATCTCTAAAGCGGACAACACGAACGATAAAGTCTTATGCCCTGATTGCTTGAGAGAGACCACGCTTTTAGAAGAAGAG
AGTTATTTTTTCAGATTGAGCGCGTATGAGAAGCCTTTATTGGATTTTTACGCTAAAAACCCCGAAGCGATTTTGCCCGT
TTATCGTAAAAATGAGGTAACTTCTTTCATTGAGCAGGGTTTATTGGATCTGTCTATCACGCGCACGAGCTTTGAATGGG
GCATTCCTTTGCCTAAAAAAATGAACGATCCTAAACATGTGGTGTATGTTTGGCTGGACGCTTTATTGAATTATGCGAGC
GCGCTAGGGTATTTGAATGGTTTAGACAATAAAATGGCGCATTTTGCATGCGCTAGGCATATCGTGGGTAAGGATATTTT
ACGCTTCCATGCCATTTATTGGCCAGCTTTTTTGATGAGTTTGAATTTGCCTTTATTCAAACAGCTTTGCGTGCATGGGT
GGTGGACGATAGAGGGCGTGAAAATGAGTAAGAGCTTGGGTAATGTTTTAGACGCTCAAAAAATCGCTATGGAGTATGGG
ATTGAGGAATTGCGTTATTTTTTATTGCGTGAGGTGCCTTTTGGGCAAGATGGGGATTTTTCTAAAAAAGCGCTAATAGA
AAGGATCAATGCGAATTTGAATAACGATTTAGGGAATTTGTTGAATCGCTTGCTAGGCATGGCTAAAAAATATTTCAATC
ATTCTCTAAAAAGCGCAAAAATCACCGCTTATTATTCTAAAGAGCTAGAAAAAGTGCATCAAATTTTAGATAACGCTAAT
TCTTTTGTGCCTAAAATGCAATTGCATAAAGCTTTAGAGGAATTGTTTAATGTCTATGATTTTTTAAACAAACTCATCGC
TAAAGAAGAGCCATGGGTTTTGCACAAAAACAACGAATCAGAAAAACTAGAAGCCTTATTGAGTTTGATCGCAAACACGC
TTTTACAATCAAGCTTTTTGCTCTATGCGTTCATGCCAAAGAGTGCGGCTAAATTAGCGAGCGCTTTTCATGCAGAGATT
ACGCCCCATAATTACGAACGCTTTTTTAAAGCTAAAAAATTACAAGATATGATTTTACAAGACACCGAGCCTTTATTTTC
CAAAATTGAGAAAATTGAAAAGGCTGAAAAAGCGGGAGAAGTCCCACCAGAAAAAGAAAAAAAAGAAGAAAAAGAAGAAA
AAGAAAAAAAAGAAAAAAAAGAAAAAAAAGAAAAAAAAGAAAAAGCCCCACTAAAACAAGAAAACTATATCAGCATTGAG
GATTTTAAAAAAGTAGAGATTAAAGTGGGGCTTATCAAAGAAGCTCAAAGGATTGAAAAATCCAATAAATTACTGCGCTT
GAAAGTGGATTTAGGCGAAGGTTGTTTGAGGCAGATTATCTCAGGGATCGCTTTGGATTATGAACCTGAAAGCCTAGTGG
GTCAAATGGTGTGCGTGGTGGCTAATTTAAAACCCGCAAAGCTTATGGGCGAAATGAGTGAGGGCATGATTTTGGCGGTG
CGAGATAGCGATAATCTAGCCTTAATCAGCCCTACCAAAGAAAAAATTGCAGGAAGTTTGATTAGCTAA

Upstream 100 bases:

>100_bases
CCCATGAATACGCATAAATTCAACCTCTATTCTCTAAATTTTTAAAAGCATGTTAAAATTAAGAGATTTCAAACAATTTT
AAGGTAACACGATTATAAAG

Downstream 100 bases:

>100_bases
ATGCGATTAGGGGTGAATGAAGCCGTAGAATTGAGTTTGGGCGAATTGCAAAACACGCCCTCAATCAGCTATTTTAATTC
TATTGTTTTGTCTTTAAACA

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 662; Mature: 662

Protein sequence:

>662_residues
MQKSLITTPIYYVNDVPHIGHAYTTLIADTLKKYYTLQGEEVFFLTGTDEHGQKIEQSARLRNQSPKAYADSISAIFKDQ
WDFFNLDYDGFIRTTDSEHQKCVQNAFEIMFEKGDIYKGAYSGYYCVSCESYCAISKADNTNDKVLCPDCLRETTLLEEE
SYFFRLSAYEKPLLDFYAKNPEAILPVYRKNEVTSFIEQGLLDLSITRTSFEWGIPLPKKMNDPKHVVYVWLDALLNYAS
ALGYLNGLDNKMAHFACARHIVGKDILRFHAIYWPAFLMSLNLPLFKQLCVHGWWTIEGVKMSKSLGNVLDAQKIAMEYG
IEELRYFLLREVPFGQDGDFSKKALIERINANLNNDLGNLLNRLLGMAKKYFNHSLKSAKITAYYSKELEKVHQILDNAN
SFVPKMQLHKALEELFNVYDFLNKLIAKEEPWVLHKNNESEKLEALLSLIANTLLQSSFLLYAFMPKSAAKLASAFHAEI
TPHNYERFFKAKKLQDMILQDTEPLFSKIEKIEKAEKAGEVPPEKEKKEEKEEKEKKEKKEKKEKKEKAPLKQENYISIE
DFKKVEIKVGLIKEAQRIEKSNKLLRLKVDLGEGCLRQIISGIALDYEPESLVGQMVCVVANLKPAKLMGEMSEGMILAV
RDSDNLALISPTKEKIAGSLIS

Sequences:

>Translated_662_residues
MQKSLITTPIYYVNDVPHIGHAYTTLIADTLKKYYTLQGEEVFFLTGTDEHGQKIEQSARLRNQSPKAYADSISAIFKDQ
WDFFNLDYDGFIRTTDSEHQKCVQNAFEIMFEKGDIYKGAYSGYYCVSCESYCAISKADNTNDKVLCPDCLRETTLLEEE
SYFFRLSAYEKPLLDFYAKNPEAILPVYRKNEVTSFIEQGLLDLSITRTSFEWGIPLPKKMNDPKHVVYVWLDALLNYAS
ALGYLNGLDNKMAHFACARHIVGKDILRFHAIYWPAFLMSLNLPLFKQLCVHGWWTIEGVKMSKSLGNVLDAQKIAMEYG
IEELRYFLLREVPFGQDGDFSKKALIERINANLNNDLGNLLNRLLGMAKKYFNHSLKSAKITAYYSKELEKVHQILDNAN
SFVPKMQLHKALEELFNVYDFLNKLIAKEEPWVLHKNNESEKLEALLSLIANTLLQSSFLLYAFMPKSAAKLASAFHAEI
TPHNYERFFKAKKLQDMILQDTEPLFSKIEKIEKAEKAGEVPPEKEKKEEKEEKEKKEKKEKKEKKEKAPLKQENYISIE
DFKKVEIKVGLIKEAQRIEKSNKLLRLKVDLGEGCLRQIISGIALDYEPESLVGQMVCVVANLKPAKLMGEMSEGMILAV
RDSDNLALISPTKEKIAGSLIS
>Mature_662_residues
MQKSLITTPIYYVNDVPHIGHAYTTLIADTLKKYYTLQGEEVFFLTGTDEHGQKIEQSARLRNQSPKAYADSISAIFKDQ
WDFFNLDYDGFIRTTDSEHQKCVQNAFEIMFEKGDIYKGAYSGYYCVSCESYCAISKADNTNDKVLCPDCLRETTLLEEE
SYFFRLSAYEKPLLDFYAKNPEAILPVYRKNEVTSFIEQGLLDLSITRTSFEWGIPLPKKMNDPKHVVYVWLDALLNYAS
ALGYLNGLDNKMAHFACARHIVGKDILRFHAIYWPAFLMSLNLPLFKQLCVHGWWTIEGVKMSKSLGNVLDAQKIAMEYG
IEELRYFLLREVPFGQDGDFSKKALIERINANLNNDLGNLLNRLLGMAKKYFNHSLKSAKITAYYSKELEKVHQILDNAN
SFVPKMQLHKALEELFNVYDFLNKLIAKEEPWVLHKNNESEKLEALLSLIANTLLQSSFLLYAFMPKSAAKLASAFHAEI
TPHNYERFFKAKKLQDMILQDTEPLFSKIEKIEKAEKAGEVPPEKEKKEEKEEKEKKEKKEKKEKKEKAPLKQENYISIE
DFKKVEIKVGLIKEAQRIEKSNKLLRLKVDLGEGCLRQIISGIALDYEPESLVGQMVCVVANLKPAKLMGEMSEGMILAV
RDSDNLALISPTKEKIAGSLIS

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI24308436, Length=503, Percent_Identity=34.7912524850895, Blast_Score=282, Evalue=7e-76,
Organism=Homo sapiens, GI14043022, Length=516, Percent_Identity=26.3565891472868, Blast_Score=150, Evalue=3e-36,
Organism=Escherichia coli, GI1788432, Length=691, Percent_Identity=25.6150506512301, Blast_Score=214, Evalue=1e-56,
Organism=Escherichia coli, GI1789455, Length=104, Percent_Identity=35.5769230769231, Blast_Score=65, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI212645096, Length=483, Percent_Identity=34.3685300207039, Blast_Score=261, Evalue=7e-70,
Organism=Caenorhabditis elegans, GI71992781, Length=373, Percent_Identity=32.9758713136729, Blast_Score=179, Evalue=6e-45,
Organism=Caenorhabditis elegans, GI17540794, Length=607, Percent_Identity=25.3706754530478, Blast_Score=169, Evalue=3e-42,
Organism=Saccharomyces cerevisiae, GI6321610, Length=480, Percent_Identity=34.1666666666667, Blast_Score=267, Evalue=4e-72,
Organism=Saccharomyces cerevisiae, GI6321704, Length=550, Percent_Identity=24.7272727272727, Blast_Score=120, Evalue=9e-28,
Organism=Drosophila melanogaster, GI21358483, Length=487, Percent_Identity=34.0862422997947, Blast_Score=270, Evalue=3e-72,
Organism=Drosophila melanogaster, GI24655311, Length=559, Percent_Identity=26.4758497316637, Blast_Score=167, Evalue=2e-41,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080 [H]

Pfam domain/function: PF09334 tRNA-synt_1g; PF01588 tRNA_bind [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 75875; Mature: 75875

Theoretical pI: Translated: 7.05; Mature: 7.05

Prosite motif: PS50886 TRBD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKSLITTPIYYVNDVPHIGHAYTTLIADTLKKYYTLQGEEVFFLTGTDEHGQKIEQSAR
CCCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHH
LRNQSPKAYADSISAIFKDQWDFFNLDYDGFIRTTDSEHQKCVQNAFEIMFEKGDIYKGA
HCCCCCHHHHHHHHHHHHCCCCEECCCCCCEEECCCHHHHHHHHHHHHHHHHCCCCCCCC
YSGYYCVSCESYCAISKADNTNDKVLCPDCLRETTLLEEESYFFRLSAYEKPLLDFYAKN
CCCEEEEECCCCEEEECCCCCCCCEECHHHHHHHHHHCCCCCEEEECHHCCHHHHHHCCC
PEAILPVYRKNEVTSFIEQGLLDLSITRTSFEWGIPLPKKMNDPKHVVYVWLDALLNYAS
CCCEECCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
ALGYLNGLDNKMAHFACARHIVGKDILRFHAIYWPAFLMSLNLPLFKQLCVHGWWTIEGV
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCEEEECC
KMSKSLGNVLDAQKIAMEYGIEELRYFLLREVPFGQDGDFSKKALIERINANLNNDLGNL
HHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCHHHHH
LNRLLGMAKKYFNHSLKSAKITAYYSKELEKVHQILDNANSFVPKMQLHKALEELFNVYD
HHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHCHHHCCHHHHHHHHHHHHHHHHH
FLNKLIAKEEPWVLHKNNESEKLEALLSLIANTLLQSSFLLYAFMPKSAAKLASAFHAEI
HHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHEEECCHHHHHHHHHHHCCC
TPHNYERFFKAKKLQDMILQDTEPLFSKIEKIEKAEKAGEVPPEKEKKEEKEEKEKKEKK
CCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
EKKEKKEKAPLKQENYISIEDFKKVEIKVGLIKEAQRIEKSNKLLRLKVDLGEGCLRQII
HHHHHHHHCCCCCCCCEEHHCCCEEEEEEHHHHHHHHHHHCCCEEEEEECCCHHHHHHHH
SGIALDYEPESLVGQMVCVVANLKPAKLMGEMSEGMILAVRDSDNLALISPTKEKIAGSL
HHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCEEEEEECCCCEEEECCCHHHHHHCC
IS
CC
>Mature Secondary Structure
MQKSLITTPIYYVNDVPHIGHAYTTLIADTLKKYYTLQGEEVFFLTGTDEHGQKIEQSAR
CCCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHH
LRNQSPKAYADSISAIFKDQWDFFNLDYDGFIRTTDSEHQKCVQNAFEIMFEKGDIYKGA
HCCCCCHHHHHHHHHHHHCCCCEECCCCCCEEECCCHHHHHHHHHHHHHHHHCCCCCCCC
YSGYYCVSCESYCAISKADNTNDKVLCPDCLRETTLLEEESYFFRLSAYEKPLLDFYAKN
CCCEEEEECCCCEEEECCCCCCCCEECHHHHHHHHHHCCCCCEEEECHHCCHHHHHHCCC
PEAILPVYRKNEVTSFIEQGLLDLSITRTSFEWGIPLPKKMNDPKHVVYVWLDALLNYAS
CCCEECCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
ALGYLNGLDNKMAHFACARHIVGKDILRFHAIYWPAFLMSLNLPLFKQLCVHGWWTIEGV
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCEEEECC
KMSKSLGNVLDAQKIAMEYGIEELRYFLLREVPFGQDGDFSKKALIERINANLNNDLGNL
HHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCHHHHH
LNRLLGMAKKYFNHSLKSAKITAYYSKELEKVHQILDNANSFVPKMQLHKALEELFNVYD
HHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHCHHHCCHHHHHHHHHHHHHHHHH
FLNKLIAKEEPWVLHKNNESEKLEALLSLIANTLLQSSFLLYAFMPKSAAKLASAFHAEI
HHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHEEECCHHHHHHHHHHHCCC
TPHNYERFFKAKKLQDMILQDTEPLFSKIEKIEKAEKAGEVPPEKEKKEEKEEKEKKEKK
CCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
EKKEKKEKAPLKQENYISIEDFKKVEIKVGLIKEAQRIEKSNKLLRLKVDLGEGCLRQII
HHHHHHHHCCCCCCCCEEHHCCCEEEEEEHHHHHHHHHHHCCCEEEEEECCCHHHHHHHH
SGIALDYEPESLVGQMVCVVANLKPAKLMGEMSEGMILAVRDSDNLALISPTKEKIAGSL
HHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCEEEEEECCCCEEEECCCHHHHHHCC
IS
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]