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The map label for this gene is glyS

Identifier: 188527791

GI number: 188527791

Start: 1013981

End: 1016083

Strand: Reverse

Name: glyS

Synonym: HPSH_05135

Alternate gene names: 188527791

Gene position: 1016083-1013981 (Counterclockwise)

Preceding gene: 188527795

Following gene: 188527790

Centisome position: 63.17

GC content: 37.66

Gene sequence:

>2103_bases
TTGCATTCAGATGAATTGTTAGTAGAGATTTTAGTTGAAGAATTGCCCGCTCAAGCGTTATTGAATGAATATAAAGAAAT
GCCTAAAAAACTCCACGCTCTTTTTAATAAGCGCGCTTTAGAAGTGGGAAAGATAGAGATTTTTTACACCCCTAGGCGTT
TGTGTTTGTTGGTTAAAGATTTCCCCCTTTTAACCCAAGAAACTAAAGAGGAATTTTTTGGGCCTCCCGTTAAAATCGCA
TGCAATCATCAAGATAAAACGCAAGGGTTGAACGAGTTAGGTTTAGGGTTTTATCAAAAATTAGGGTTAAAAGATCATCA
GCATTTCCAAACAGCGTTCAAAAACAATAACGAGGTGCTTTATCACGCTAAAATCCATGCGAAAGAGCCTACAAAAGACT
TAATCATGCCCATTGTGTTAGAGTTTTTAGAGGGTTTGAATTTCGGGAAGTCTATGCGTTGGGGCAATGTGGAAAAAAGC
TTTATCAGACCCATTCATAATATTTGCGTGTTGTTTAATGGGGAAAATTTTAACGATATTGAAGTCAAAGAGTATGGCTT
TAAAACCAAACAAGCCACGAAAGCGCACCGACAAGAGGGTTTTGATTTTATCCAAGTGGATAGCCCTAAAGCCTATTTTG
AAGTTTTAGAAAAAAACCATGTCATTTTAGACCCTAAAAAGCGCAAAGCTAAAATCTTACAAGAAATTAAAGAGCTAGAA
ACAAAGCATCGCATCATCGTAGAAATAGATAGGGATTTATTAGATGAGGTGGTAGCAATCACGGAATACCCCAGTGCGCT
TTTAGGGGAGTTTGACAAGGCGTTTTTAAAATTACCTAATGAAATCATCATTACTTCCATGAAAGAAAACCAACGCTATT
TTGCAGCCTTTAATCAAAAAAGCCAAGAAAGCCCAACATTACACAACGGCTTTATTGTGGTGAGTAACGCTATCAGTAAA
GACAAGCAAAAAATCATTGCAGGCAATCAAAAGGTTTTAAAAGCCCGTTTGAGCGATGCGGTTTTCTTTTATGAAAACGA
TCTCAAAAAGCCTTTAGATAACGCCCCTTTAGAGAGCGTGGTTTTTGTGCAAGGCTTAGGGACTTTAAAGGACAAAATGG
AGCGAGAAGCGATCATCGCTCAATATTTGACGCAAAAATACGCTTCATCTTTAAACATGCCTTTAGAAAAAGCCCTTGAA
TTGGTTGGCCGAGCCGTTAGAATCGCTAAGGCGGATTTACTCAGTGAAGTGGTGTATGAATTTAGCGAGCTTCAAGGGAT
CATGGGCTATTACTACGCTTTAAAACAAAATGAAAACGAATTAGTCGCCTTGAGTGTGAAAGAGCAGTATTTGCCCGCAA
GCGAAAACGCTCCCTTACCCTCTAGCGTTTTTAGCGCGATCGTGGCTTTGAGCTTGAAATTAGACAGCCTGTTTTCTCTT
TTTAGCGTGGGTAAAATCCCTAGCGGATCTAAAGATCCTTTTGCTTTAAGGCGCTTGAGTTTTGGGCTATTGAAAATCGT
TGTGCATTATGGGTTGGAATTTGATTTGAAAGCGGATTTAAAAAACCTCTTTGAAAAAGTGGGCGTTTATCAAAGCTTTG
ATTTAGAGGTTTTAGAAAAGTTTTTACTGGAGCGCTTTAATAATTTAATAGATTGTAACCCCTCTATCATAAGGAGCGTG
TTAAACACCAACGAGCGAGGTATTGTTAAAATCATTCAAAAAGTCAAAGCCTTAAAACGCTTTTTAGACGATCCCAAGAA
CGCTCAAAAAAAAGAGTTGCTTTTTAGCGCTTTTAAACGCCTAGCCAATATCAATAAAGACAGAAACCCTAACGAATCAA
GCGAGTTTTTTATAAGTCTTTTCAAAGAATCGCAAGAGCATGCCCTTTTTGAAGCGTTCAATGCGATCAAAACAAGCACT
TTTGAGAGTTTGGATAGCAAAATAGAGGCTTATTTTGGTTTGCATGCACCTTTAGAAGAATATTTTAAAAGCGTGCTGGT
GATGGATAAAGATATAGAAATCCAAAAAAATCGTAAAAATTTCTTGTGGGGCGTGTATCAAAGTTTCTTAGAAATTGGGG
ATATTAAAGAAATTGCGATTTAG

Upstream 100 bases:

>100_bases
TTTGTATTGAGAAATAAACTGAACTAAGAATAAATTCAAGTATAATTCTAACATGATTTAGTCTTAAAAGTGATTTTGTT
TTTTAGGGAGAATTGAGATT

Downstream 100 bases:

>100_bases
CGGCTTCAAACGTTGCCAAAGGGTGTGGCGTTTTTGTTTGGGGGCAACTTTTTTGATGAGCATGTTTAGTGCAGGCATGC
TTGATGCAAAAACCTTTACC

Product: glycyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS [H]

Number of amino acids: Translated: 700; Mature: 700

Protein sequence:

>700_residues
MHSDELLVEILVEELPAQALLNEYKEMPKKLHALFNKRALEVGKIEIFYTPRRLCLLVKDFPLLTQETKEEFFGPPVKIA
CNHQDKTQGLNELGLGFYQKLGLKDHQHFQTAFKNNNEVLYHAKIHAKEPTKDLIMPIVLEFLEGLNFGKSMRWGNVEKS
FIRPIHNICVLFNGENFNDIEVKEYGFKTKQATKAHRQEGFDFIQVDSPKAYFEVLEKNHVILDPKKRKAKILQEIKELE
TKHRIIVEIDRDLLDEVVAITEYPSALLGEFDKAFLKLPNEIIITSMKENQRYFAAFNQKSQESPTLHNGFIVVSNAISK
DKQKIIAGNQKVLKARLSDAVFFYENDLKKPLDNAPLESVVFVQGLGTLKDKMEREAIIAQYLTQKYASSLNMPLEKALE
LVGRAVRIAKADLLSEVVYEFSELQGIMGYYYALKQNENELVALSVKEQYLPASENAPLPSSVFSAIVALSLKLDSLFSL
FSVGKIPSGSKDPFALRRLSFGLLKIVVHYGLEFDLKADLKNLFEKVGVYQSFDLEVLEKFLLERFNNLIDCNPSIIRSV
LNTNERGIVKIIQKVKALKRFLDDPKNAQKKELLFSAFKRLANINKDRNPNESSEFFISLFKESQEHALFEAFNAIKTST
FESLDSKIEAYFGLHAPLEEYFKSVLVMDKDIEIQKNRKNFLWGVYQSFLEIGDIKEIAI

Sequences:

>Translated_700_residues
MHSDELLVEILVEELPAQALLNEYKEMPKKLHALFNKRALEVGKIEIFYTPRRLCLLVKDFPLLTQETKEEFFGPPVKIA
CNHQDKTQGLNELGLGFYQKLGLKDHQHFQTAFKNNNEVLYHAKIHAKEPTKDLIMPIVLEFLEGLNFGKSMRWGNVEKS
FIRPIHNICVLFNGENFNDIEVKEYGFKTKQATKAHRQEGFDFIQVDSPKAYFEVLEKNHVILDPKKRKAKILQEIKELE
TKHRIIVEIDRDLLDEVVAITEYPSALLGEFDKAFLKLPNEIIITSMKENQRYFAAFNQKSQESPTLHNGFIVVSNAISK
DKQKIIAGNQKVLKARLSDAVFFYENDLKKPLDNAPLESVVFVQGLGTLKDKMEREAIIAQYLTQKYASSLNMPLEKALE
LVGRAVRIAKADLLSEVVYEFSELQGIMGYYYALKQNENELVALSVKEQYLPASENAPLPSSVFSAIVALSLKLDSLFSL
FSVGKIPSGSKDPFALRRLSFGLLKIVVHYGLEFDLKADLKNLFEKVGVYQSFDLEVLEKFLLERFNNLIDCNPSIIRSV
LNTNERGIVKIIQKVKALKRFLDDPKNAQKKELLFSAFKRLANINKDRNPNESSEFFISLFKESQEHALFEAFNAIKTST
FESLDSKIEAYFGLHAPLEEYFKSVLVMDKDIEIQKNRKNFLWGVYQSFLEIGDIKEIAI
>Mature_700_residues
MHSDELLVEILVEELPAQALLNEYKEMPKKLHALFNKRALEVGKIEIFYTPRRLCLLVKDFPLLTQETKEEFFGPPVKIA
CNHQDKTQGLNELGLGFYQKLGLKDHQHFQTAFKNNNEVLYHAKIHAKEPTKDLIMPIVLEFLEGLNFGKSMRWGNVEKS
FIRPIHNICVLFNGENFNDIEVKEYGFKTKQATKAHRQEGFDFIQVDSPKAYFEVLEKNHVILDPKKRKAKILQEIKELE
TKHRIIVEIDRDLLDEVVAITEYPSALLGEFDKAFLKLPNEIIITSMKENQRYFAAFNQKSQESPTLHNGFIVVSNAISK
DKQKIIAGNQKVLKARLSDAVFFYENDLKKPLDNAPLESVVFVQGLGTLKDKMEREAIIAQYLTQKYASSLNMPLEKALE
LVGRAVRIAKADLLSEVVYEFSELQGIMGYYYALKQNENELVALSVKEQYLPASENAPLPSSVFSAIVALSLKLDSLFSL
FSVGKIPSGSKDPFALRRLSFGLLKIVVHYGLEFDLKADLKNLFEKVGVYQSFDLEVLEKFLLERFNNLIDCNPSIIRSV
LNTNERGIVKIIQKVKALKRFLDDPKNAQKKELLFSAFKRLANINKDRNPNESSEFFISLFKESQEHALFEAFNAIKTST
FESLDSKIEAYFGLHAPLEEYFKSVLVMDKDIEIQKNRKNFLWGVYQSFLEIGDIKEIAI

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1789982, Length=716, Percent_Identity=30.7262569832402, Blast_Score=295, Evalue=5e-81,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311 [H]

Pfam domain/function: PF02092 tRNA_synt_2f [H]

EC number: =6.1.1.14 [H]

Molecular weight: Translated: 80303; Mature: 80303

Theoretical pI: Translated: 7.25; Mature: 7.25

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHSDELLVEILVEELPAQALLNEYKEMPKKLHALFNKRALEVGKIEIFYTPRRLCLLVKD
CCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCEECCEEEEECCHHHHHHHHC
FPLLTQETKEEFFGPPVKIACNHQDKTQGLNELGLGFYQKLGLKDHQHFQTAFKNNNEVL
CCHHHHHHHHHHCCCCEEEEECCCHHHCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEE
YHAKIHAKEPTKDLIMPIVLEFLEGLNFGKSMRWGNVEKSFIRPIHNICVLFNGENFNDI
EEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHEEEEECCCCCCCC
EVKEYGFKTKQATKAHRQEGFDFIQVDSPKAYFEVLEKNHVILDPKKRKAKILQEIKELE
CHHHHCCCHHHHHHHHHHCCCCEEEECCCHHHHHHHHCCCEEECCCHHHHHHHHHHHHHH
TKHRIIVEIDRDLLDEVVAITEYPSALLGEFDKAFLKLPNEIIITSMKENQRYFAAFNQK
HHCCEEEEECHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHEEEECCCCCHHHHHHCCC
SQESPTLHNGFIVVSNAISKDKQKIIAGNQKVLKARLSDAVFFYENDLKKPLDNAPLESV
CCCCCCCCCCEEEEECHHHHHHHHHHCCCHHHHHHHHHHHEEEEHHHHCCCCCCCCHHHH
VFVQGLGTLKDKMEREAIIAQYLTQKYASSLNMPLEKALELVGRAVRIAKADLLSEVVYE
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
FSELQGIMGYYYALKQNENELVALSVKEQYLPASENAPLPSSVFSAIVALSLKLDSLFSL
HHHHHHHHHHHHHHCCCCCCEEEEEEHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
FSVGKIPSGSKDPFALRRLSFGLLKIVVHYGLEFDLKADLKNLFEKVGVYQSFDLEVLEK
HHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHCCCCCCCHHHHHH
FLLERFNNLIDCNPSIIRSVLNTNERGIVKIIQKVKALKRFLDDPKNAQKKELLFSAFKR
HHHHHHCHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
LANINKDRNPNESSEFFISLFKESQEHALFEAFNAIKTSTFESLDSKIEAYFGLHAPLEE
HHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
YFKSVLVMDKDIEIQKNRKNFLWGVYQSFLEIGDIKEIAI
HHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHCC
>Mature Secondary Structure
MHSDELLVEILVEELPAQALLNEYKEMPKKLHALFNKRALEVGKIEIFYTPRRLCLLVKD
CCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCEECCEEEEECCHHHHHHHHC
FPLLTQETKEEFFGPPVKIACNHQDKTQGLNELGLGFYQKLGLKDHQHFQTAFKNNNEVL
CCHHHHHHHHHHCCCCEEEEECCCHHHCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEE
YHAKIHAKEPTKDLIMPIVLEFLEGLNFGKSMRWGNVEKSFIRPIHNICVLFNGENFNDI
EEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHEEEEECCCCCCCC
EVKEYGFKTKQATKAHRQEGFDFIQVDSPKAYFEVLEKNHVILDPKKRKAKILQEIKELE
CHHHHCCCHHHHHHHHHHCCCCEEEECCCHHHHHHHHCCCEEECCCHHHHHHHHHHHHHH
TKHRIIVEIDRDLLDEVVAITEYPSALLGEFDKAFLKLPNEIIITSMKENQRYFAAFNQK
HHCCEEEEECHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHEEEECCCCCHHHHHHCCC
SQESPTLHNGFIVVSNAISKDKQKIIAGNQKVLKARLSDAVFFYENDLKKPLDNAPLESV
CCCCCCCCCCEEEEECHHHHHHHHHHCCCHHHHHHHHHHHEEEEHHHHCCCCCCCCHHHH
VFVQGLGTLKDKMEREAIIAQYLTQKYASSLNMPLEKALELVGRAVRIAKADLLSEVVYE
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
FSELQGIMGYYYALKQNENELVALSVKEQYLPASENAPLPSSVFSAIVALSLKLDSLFSL
HHHHHHHHHHHHHHCCCCCCEEEEEEHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
FSVGKIPSGSKDPFALRRLSFGLLKIVVHYGLEFDLKADLKNLFEKVGVYQSFDLEVLEK
HHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHCCCCCCCHHHHHH
FLLERFNNLIDCNPSIIRSVLNTNERGIVKIIQKVKALKRFLDDPKNAQKKELLFSAFKR
HHHHHHCHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
LANINKDRNPNESSEFFISLFKESQEHALFEAFNAIKTSTFESLDSKIEAYFGLHAPLEE
HHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
YFKSVLVMDKDIEIQKNRKNFLWGVYQSFLEIGDIKEIAI
HHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA