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The map label for this gene is virD4 [H]

Identifier: 188527687

GI number: 188527687

Start: 897362

End: 899089

Strand: Reverse

Name: virD4 [H]

Synonym: HPSH_04545

Alternate gene names: 188527687

Gene position: 899089-897362 (Counterclockwise)

Preceding gene: 188527688

Following gene: 188527684

Centisome position: 55.89

GC content: 33.97

Gene sequence:

>1728_bases
ATGAGAGAAAAATTCAAAACATTAAGAGATAAATTTAAGGATAAAACCAACAATTTATTCCATAAGCTTAATAGCATTCA
AAAAGATTATTACACTAAAACTTTCCCTAAGGCTTGCAAAGATGTCTTTACGCTAGGTTCTTCTAAGAGTTATCCTATGA
CCTTAAATTTTAACAAAGGTTTTTGTGTGGGGCTATATAAAGGTTTAAATTCACTTAAGCCTACTTATTATGATGCTCCG
CTTTCTACTTTAATTATCGCTCCTCCTGGAAGTGGTAAAACCGCAGCTGTGGCTGTGCCTAATCTTTTAAATGTGCCTAG
CTCTTGTGTGGTGTTAGATATTAAAGGGGAGTTGTTTGATTTAACGGCTGGTTACAGACAACAAGTTCTAAAAAATAAAA
TTTTTGTATTTGATCCCTTAGGAAATGATAACACGCTTAAATTCAATCCGTTTGATAAACGCATTGTAGAAAAATTAGAT
TTTAACAGAAAGCGAAGGCTTGTAGATGAAGTGGGTAATACTATTTTTGCTGAAGATGGGACAAATAAAGACCCTCATTG
GACACAGCAAGCTAAAAATTTATTTGTCTTTTACGCTCTTTATGATTTGTGTGTGCATAACACTAGCACCTTTTTTGAAA
TTGCAAGCGCTCCTATAAAAAATTATGTCCCCTTAATCAATCCACAAAGCCGATTTTATACTGAATTATATGAATGTCAA
AGTAGTGATAATGGTTTTGTTAAAGAGAATGGGCGTTATATGGCTAAAGTAGAAAATGGCGTTAAAAAAATGAAACCTAA
TGTCAATGTGGAATTGCTATGGTATAAACAAGTAGCCGAACAAGTCTATACTGACCCAGAAAATCCTAAAAATTATGATG
GATCTGTGAATAATTTAGAAAAAGACGATCAAGGCAATGTTATAATGAAAGAGGGCATGTTAGATCCTGTTATTAGAAAC
GAAGCGAACAAATGGGCTAAGGCGAATGATAAAGAGTTTGCGAGCATTAAATCAGTTTATAGCCGTTTTATGCAAGTCTT
TACAAGCTATCAAGTTAAAAGCGCTACAGATAGCATGAGTTTTGAATACGAGGATTTAAGGGCTGACAATATTTCACTCT
ATATTAAAATCGCTCAAACAGATATTGACACACTCGCACCGCTTATCCGTATCCTTTTGGAAAGTATTGCTAAAAATCTT
TTATTGAAAGAGAGTAAGAAATTTGAAGAAAGGGTTTATCTGTTTTTAGATGAATTTGTGCGTTTTGGTAAATTGCCTTT
TCTATTAGAAATGCCCGCATTAAGTAGGAGCTATGGTGTGGTTTTAATTTTTATCACGCAATCCAACGCTCTTATTGAAA
AATATTATGGTAGAGAAGATGCAAGAATTGTTAATAGCACCGTGGCTTACAAAGTAATTTTCAAAATGGATGATTTAGAA
TATGCTAAACAAGTGAGCGAAGAAGTCGGTAAGATGACTAGAAAAACACGAAGCCACTCTACAGAAAAAGGACAACTCAT
TACCGGAGGAACTTCTAGTATAGGTAAAGAGGCGTGGGACTTATTGAGCGCGCAAGATATTATGAATATTGATAAAGATG
AAGTGATCATTTTAGTAAGCGGTCATAAGGCTAAACCCTTAAAATTAAAAGCGAATTATTATTTCAAAAACAAAGAATTA
CTCTCTCGTATTAACTGGGAAGTCAAGCCCAATGAAGAAGTGTTTTGA

Upstream 100 bases:

>100_bases
ATCTTTTTTAGTAGTGACTTGTGTCTTAACCTTTATATTTAGGGGATTTTTAAGTGTAAAACTAACGCTATTTATGAAAT
ATCTAAAAGGATTATTTTAA

Downstream 100 bases:

>100_bases
TGAATTAAAAAAGTTTGCATGAGCGCTTTTTAATGGCTTTTTAAAAATCGCTTTCTGCAACTTCTTATCCTTGTTGCTAA
AAAAGAATGCGCCAATTTCT

Product: conjugal transfer protein (traG)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 575; Mature: 575

Protein sequence:

>575_residues
MREKFKTLRDKFKDKTNNLFHKLNSIQKDYYTKTFPKACKDVFTLGSSKSYPMTLNFNKGFCVGLYKGLNSLKPTYYDAP
LSTLIIAPPGSGKTAAVAVPNLLNVPSSCVVLDIKGELFDLTAGYRQQVLKNKIFVFDPLGNDNTLKFNPFDKRIVEKLD
FNRKRRLVDEVGNTIFAEDGTNKDPHWTQQAKNLFVFYALYDLCVHNTSTFFEIASAPIKNYVPLINPQSRFYTELYECQ
SSDNGFVKENGRYMAKVENGVKKMKPNVNVELLWYKQVAEQVYTDPENPKNYDGSVNNLEKDDQGNVIMKEGMLDPVIRN
EANKWAKANDKEFASIKSVYSRFMQVFTSYQVKSATDSMSFEYEDLRADNISLYIKIAQTDIDTLAPLIRILLESIAKNL
LLKESKKFEERVYLFLDEFVRFGKLPFLLEMPALSRSYGVVLIFITQSNALIEKYYGREDARIVNSTVAYKVIFKMDDLE
YAKQVSEEVGKMTRKTRSHSTEKGQLITGGTSSIGKEAWDLLSAQDIMNIDKDEVIILVSGHKAKPLKLKANYYFKNKEL
LSRINWEVKPNEEVF

Sequences:

>Translated_575_residues
MREKFKTLRDKFKDKTNNLFHKLNSIQKDYYTKTFPKACKDVFTLGSSKSYPMTLNFNKGFCVGLYKGLNSLKPTYYDAP
LSTLIIAPPGSGKTAAVAVPNLLNVPSSCVVLDIKGELFDLTAGYRQQVLKNKIFVFDPLGNDNTLKFNPFDKRIVEKLD
FNRKRRLVDEVGNTIFAEDGTNKDPHWTQQAKNLFVFYALYDLCVHNTSTFFEIASAPIKNYVPLINPQSRFYTELYECQ
SSDNGFVKENGRYMAKVENGVKKMKPNVNVELLWYKQVAEQVYTDPENPKNYDGSVNNLEKDDQGNVIMKEGMLDPVIRN
EANKWAKANDKEFASIKSVYSRFMQVFTSYQVKSATDSMSFEYEDLRADNISLYIKIAQTDIDTLAPLIRILLESIAKNL
LLKESKKFEERVYLFLDEFVRFGKLPFLLEMPALSRSYGVVLIFITQSNALIEKYYGREDARIVNSTVAYKVIFKMDDLE
YAKQVSEEVGKMTRKTRSHSTEKGQLITGGTSSIGKEAWDLLSAQDIMNIDKDEVIILVSGHKAKPLKLKANYYFKNKEL
LSRINWEVKPNEEVF
>Mature_575_residues
MREKFKTLRDKFKDKTNNLFHKLNSIQKDYYTKTFPKACKDVFTLGSSKSYPMTLNFNKGFCVGLYKGLNSLKPTYYDAP
LSTLIIAPPGSGKTAAVAVPNLLNVPSSCVVLDIKGELFDLTAGYRQQVLKNKIFVFDPLGNDNTLKFNPFDKRIVEKLD
FNRKRRLVDEVGNTIFAEDGTNKDPHWTQQAKNLFVFYALYDLCVHNTSTFFEIASAPIKNYVPLINPQSRFYTELYECQ
SSDNGFVKENGRYMAKVENGVKKMKPNVNVELLWYKQVAEQVYTDPENPKNYDGSVNNLEKDDQGNVIMKEGMLDPVIRN
EANKWAKANDKEFASIKSVYSRFMQVFTSYQVKSATDSMSFEYEDLRADNISLYIKIAQTDIDTLAPLIRILLESIAKNL
LLKESKKFEERVYLFLDEFVRFGKLPFLLEMPALSRSYGVVLIFITQSNALIEKYYGREDARIVNSTVAYKVIFKMDDLE
YAKQVSEEVGKMTRKTRSHSTEKGQLITGGTSSIGKEAWDLLSAQDIMNIDKDEVIILVSGHKAKPLKLKANYYFKNKEL
LSRINWEVKPNEEVF

Specific function: The type IV secretion system VirB/VirD4 is a major virulence determinant for subversion of human endothelial cell (HEC) function. VirB-dependent changes of HEC include massive cytoskeletal rearrangements, a proinflammatory activation by nuclear factor NF-

COG id: COG3505

COG function: function code U; Type IV secretory pathway, VirD4 components

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the virD4/traG family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014135
- InterPro:   IPR003688 [H]

Pfam domain/function: PF02534 TraG [H]

EC number: NA

Molecular weight: Translated: 65966; Mature: 65966

Theoretical pI: Translated: 9.23; Mature: 9.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MREKFKTLRDKFKDKTNNLFHKLNSIQKDYYTKTFPKACKDVFTLGSSKSYPMTLNFNKG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCC
FCVGLYKGLNSLKPTYYDAPLSTLIIAPPGSGKTAAVAVPNLLNVPSSCVVLDIKGELFD
EEHHHHHHHHHCCCCEECCCCEEEEEECCCCCCEEEEECCHHHCCCCCEEEEEECCCEEE
LTAGYRQQVLKNKIFVFDPLGNDNTLKFNPFDKRIVEKLDFNRKRRLVDEVGNTIFAEDG
CCHHHHHHHHHCCEEEEECCCCCCEEEECCHHHHHHHHHCCCHHHHHHHHHCCEEEECCC
TNKDPHWTQQAKNLFVFYALYDLCVHNTSTFFEIASAPIKNYVPLINPQSRFYTELYECQ
CCCCCCHHHHHHHEEHHHHHHHHHHCCCCHHHHHHCCCHHHCCCCCCCHHHHHHHHHHHC
SSDNGFVKENGRYMAKVENGVKKMKPNVNVELLWYKQVAEQVYTDPENPKNYDGSVNNLE
CCCCCEEECCCCEEEHHHHHHHHHCCCCCEEEEEHHHHHHHHHCCCCCCCCCCCCCCCCC
KDDQGNVIMKEGMLDPVIRNEANKWAKANDKEFASIKSVYSRFMQVFTSYQVKSATDSMS
CCCCCCEEEECCCCCHHHHCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
FEYEDLRADNISLYIKIAQTDIDTLAPLIRILLESIAKNLLLKESKKFEERVYLFLDEFV
CCHHHCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RFGKLPFLLEMPALSRSYGVVLIFITQSNALIEKYYGREDARIVNSTVAYKVIFKMDDLE
HHCCCCEEEECCCCCCCCCEEEEEEECCCHHHHHHCCCCCHHHHHHHEEEEEEEEECCHH
YAKQVSEEVGKMTRKTRSHSTEKGQLITGGTSSIGKEAWDLLSAQDIMNIDKDEVIILVS
HHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEE
GHKAKPLKLKANYYFKNKELLSRINWEVKPNEEVF
CCCCCCEEEEEEEEECCHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MREKFKTLRDKFKDKTNNLFHKLNSIQKDYYTKTFPKACKDVFTLGSSKSYPMTLNFNKG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCC
FCVGLYKGLNSLKPTYYDAPLSTLIIAPPGSGKTAAVAVPNLLNVPSSCVVLDIKGELFD
EEHHHHHHHHHCCCCEECCCCEEEEEECCCCCCEEEEECCHHHCCCCCEEEEEECCCEEE
LTAGYRQQVLKNKIFVFDPLGNDNTLKFNPFDKRIVEKLDFNRKRRLVDEVGNTIFAEDG
CCHHHHHHHHHCCEEEEECCCCCCEEEECCHHHHHHHHHCCCHHHHHHHHHCCEEEECCC
TNKDPHWTQQAKNLFVFYALYDLCVHNTSTFFEIASAPIKNYVPLINPQSRFYTELYECQ
CCCCCCHHHHHHHEEHHHHHHHHHHCCCCHHHHHHCCCHHHCCCCCCCHHHHHHHHHHHC
SSDNGFVKENGRYMAKVENGVKKMKPNVNVELLWYKQVAEQVYTDPENPKNYDGSVNNLE
CCCCCEEECCCCEEEHHHHHHHHHCCCCCEEEEEHHHHHHHHHCCCCCCCCCCCCCCCCC
KDDQGNVIMKEGMLDPVIRNEANKWAKANDKEFASIKSVYSRFMQVFTSYQVKSATDSMS
CCCCCCEEEECCCCCHHHHCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
FEYEDLRADNISLYIKIAQTDIDTLAPLIRILLESIAKNLLLKESKKFEERVYLFLDEFV
CCHHHCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RFGKLPFLLEMPALSRSYGVVLIFITQSNALIEKYYGREDARIVNSTVAYKVIFKMDDLE
HHCCCCEEEECCCCCCCCCEEEEEEECCCHHHHHHCCCCCHHHHHHHEEEEEEEEECCHH
YAKQVSEEVGKMTRKTRSHSTEKGQLITGGTSSIGKEAWDLLSAQDIMNIDKDEVIILVS
HHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEE
GHKAKPLKLKANYYFKNKELLSRINWEVKPNEEVF
CCCCCCEEEEEEEEECCHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA