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The map label for this gene is glcD [H]

Identifier: 188527654

GI number: 188527654

Start: 855403

End: 856782

Strand: Reverse

Name: glcD [H]

Synonym: HPSH_04380

Alternate gene names: 188527654

Gene position: 856782-855403 (Counterclockwise)

Preceding gene: 188527655

Following gene: 188527653

Centisome position: 53.26

GC content: 42.75

Gene sequence:

>1380_bases
ATGTTAGAGAAACAACACATCCAATATTTTAAAAACCTGGTAGGGGGAGAGGATTTTTTCACTGATTTAGCGCATTTGAA
CGCTTATTGCTATGACGCTACCAAAGAAAGGCATTTGCCTAGTGGTGTGATTTTCCCTAAAAACGAGCAAGAAATCAGCC
AGATTTTAAAATATTGCAACGAACATCGCCTTATCGTTGTGCCTAGGGGGGCTGGGAGCGGTTTTACCGGGGGGGCGTTG
AGCGTGAGCGGAGGGCTAGTTTTAAGCGTAGAAAAGCATTTGGATAAGATTTTAGAGATTGACACTAAAAATTTAATCGC
TAGAGTAGAACCGGGCGTGATCAACAAGCATTTCCAAAACGAAGTGGAAAAATTGAATCTCTTCTACCCCCCAGATCCAG
CGAGTGAGAATCAAAGCACTTTAGGGGGGAATGCCGCTGAAAACGCCGGTGGCATGCGCGCGGCTAAATACGGCATCACG
AAAGATTATGTCATGGCGATGAGAGTGGTTTTAGCGAATGGTGAAATCATAAGGGCAGGCAAAAAAACGATCAAAGATGT
CGCCGGTTTTAATGTTGCAGGGCTGATGATCGCTAGTGAGGGGTGCTTGGGCGTGATTTCTGAAATCACTTTAAAGCTTT
TAGCCAAACCACTTCTAAAACAAAGCGCGATGGGGGTTTTTAACCATATTGAAGACGCCATGAGCGCTGTTTATAAGACA
ATGAGCAGTGGCGTTACGCCTGTGGCGATGGAATTTTTGGATAATTTGAGTATCAAAGCGGTTGAAGAACGATTTTCTAA
AGGCTTACCCAAAGACGCTGGAGCGATACTCATCACTCAAGTGGATGGCGTGGTTAAAGAGCAGATTGCATGGCAACTCA
ATGAGATAGAAAAGCATTTTAAAGCCAATGGGTGCGTGGGTTTTAAGATTGCTCAAAACGAACAAGAAGAGCAGGATTTA
TGGTTTTCAAGGCGTAACGCTTCTCAAAGTATTAGCGTTTATGGTAAAAAGAAATTGAATGAAGATGTGACCGTTCCTAG
GGCGAGTTTGCCGAGTTTGTTGCAAGAAGTTGCTAAAATAAGCCAAAAATACGGCTTTAAAATCCCTTGCTTTGGGCATA
CGGGCGATGGTAATGTGCATGTGAATATCATGCTAGAAGATCCTAAAAGGGATTTAGAAAAAGGGCATCAGGCTATGGAA
GAGATTTTTCAGGCCGCTATCAGTTTGGAGGGGACTTTAAGCGGGGAGCATGGCATAGGCTTGTCTAAAGCCAAATTCAT
GCCTTTAGCGTTCAATCATAGTGAAATGGAGCTTTTTAGGAATATTAAAAAAGCCCTTGATCCCAATAATATTTTAAACC
CTTTTAAAATGGGGTTGTAA

Upstream 100 bases:

>100_bases
AGAGCGAGAGAATTTGAACAAAGAGCCAAAGAGCATCAAGAAAGAGATGAAAAAGAGCTTGAAGAGCGAAGAAAGGCGCT
AGAAGCGGGTAAAAAATAAC

Downstream 100 bases:

>100_bases
GATTGAAAAGCAAGGAAAGCGCATGAAAATCGGTGTTTATGGAGCGAGCGGTCGTATAGGGAAACTGCTTTTAGAAGAAT
TAAAAGGGGGGTATAAGGGA

Product: putative Glycolate oxidase

Products: 2-oxo acid; H2O2

Alternate protein names: NA

Number of amino acids: Translated: 459; Mature: 459

Protein sequence:

>459_residues
MLEKQHIQYFKNLVGGEDFFTDLAHLNAYCYDATKERHLPSGVIFPKNEQEISQILKYCNEHRLIVVPRGAGSGFTGGAL
SVSGGLVLSVEKHLDKILEIDTKNLIARVEPGVINKHFQNEVEKLNLFYPPDPASENQSTLGGNAAENAGGMRAAKYGIT
KDYVMAMRVVLANGEIIRAGKKTIKDVAGFNVAGLMIASEGCLGVISEITLKLLAKPLLKQSAMGVFNHIEDAMSAVYKT
MSSGVTPVAMEFLDNLSIKAVEERFSKGLPKDAGAILITQVDGVVKEQIAWQLNEIEKHFKANGCVGFKIAQNEQEEQDL
WFSRRNASQSISVYGKKKLNEDVTVPRASLPSLLQEVAKISQKYGFKIPCFGHTGDGNVHVNIMLEDPKRDLEKGHQAME
EIFQAAISLEGTLSGEHGIGLSKAKFMPLAFNHSEMELFRNIKKALDPNNILNPFKMGL

Sequences:

>Translated_459_residues
MLEKQHIQYFKNLVGGEDFFTDLAHLNAYCYDATKERHLPSGVIFPKNEQEISQILKYCNEHRLIVVPRGAGSGFTGGAL
SVSGGLVLSVEKHLDKILEIDTKNLIARVEPGVINKHFQNEVEKLNLFYPPDPASENQSTLGGNAAENAGGMRAAKYGIT
KDYVMAMRVVLANGEIIRAGKKTIKDVAGFNVAGLMIASEGCLGVISEITLKLLAKPLLKQSAMGVFNHIEDAMSAVYKT
MSSGVTPVAMEFLDNLSIKAVEERFSKGLPKDAGAILITQVDGVVKEQIAWQLNEIEKHFKANGCVGFKIAQNEQEEQDL
WFSRRNASQSISVYGKKKLNEDVTVPRASLPSLLQEVAKISQKYGFKIPCFGHTGDGNVHVNIMLEDPKRDLEKGHQAME
EIFQAAISLEGTLSGEHGIGLSKAKFMPLAFNHSEMELFRNIKKALDPNNILNPFKMGL
>Mature_459_residues
MLEKQHIQYFKNLVGGEDFFTDLAHLNAYCYDATKERHLPSGVIFPKNEQEISQILKYCNEHRLIVVPRGAGSGFTGGAL
SVSGGLVLSVEKHLDKILEIDTKNLIARVEPGVINKHFQNEVEKLNLFYPPDPASENQSTLGGNAAENAGGMRAAKYGIT
KDYVMAMRVVLANGEIIRAGKKTIKDVAGFNVAGLMIASEGCLGVISEITLKLLAKPLLKQSAMGVFNHIEDAMSAVYKT
MSSGVTPVAMEFLDNLSIKAVEERFSKGLPKDAGAILITQVDGVVKEQIAWQLNEIEKHFKANGCVGFKIAQNEQEEQDL
WFSRRNASQSISVYGKKKLNEDVTVPRASLPSLLQEVAKISQKYGFKIPCFGHTGDGNVHVNIMLEDPKRDLEKGHQAME
EIFQAAISLEGTLSGEHGIGLSKAKFMPLAFNHSEMELFRNIKKALDPNNILNPFKMGL

Specific function: Unknown

COG id: COG0277

COG function: function code C; FAD/FMN-containing dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain [H]

Homologues:

Organism=Homo sapiens, GI37595756, Length=463, Percent_Identity=29.5896328293737, Blast_Score=221, Evalue=8e-58,
Organism=Homo sapiens, GI37595754, Length=486, Percent_Identity=27.9835390946502, Blast_Score=210, Evalue=2e-54,
Organism=Homo sapiens, GI119964728, Length=464, Percent_Identity=26.7241379310345, Blast_Score=164, Evalue=2e-40,
Organism=Homo sapiens, GI4501993, Length=255, Percent_Identity=23.5294117647059, Blast_Score=92, Evalue=1e-18,
Organism=Escherichia coli, GI1789351, Length=447, Percent_Identity=34.8993288590604, Blast_Score=277, Evalue=1e-75,
Organism=Escherichia coli, GI1787977, Length=500, Percent_Identity=23.6, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI48994907, Length=217, Percent_Identity=25.3456221198157, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI48994914, Length=174, Percent_Identity=27.0114942528736, Blast_Score=74, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI71992373, Length=425, Percent_Identity=30.8235294117647, Blast_Score=214, Evalue=8e-56,
Organism=Caenorhabditis elegans, GI71992381, Length=425, Percent_Identity=29.4117647058824, Blast_Score=191, Evalue=8e-49,
Organism=Caenorhabditis elegans, GI71992392, Length=385, Percent_Identity=29.3506493506493, Blast_Score=175, Evalue=4e-44,
Organism=Caenorhabditis elegans, GI17534361, Length=457, Percent_Identity=27.5711159737418, Blast_Score=167, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI17556096, Length=456, Percent_Identity=25.4385964912281, Blast_Score=122, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6320027, Length=427, Percent_Identity=28.8056206088993, Blast_Score=196, Evalue=6e-51,
Organism=Saccharomyces cerevisiae, GI6320023, Length=479, Percent_Identity=27.5574112734864, Blast_Score=180, Evalue=5e-46,
Organism=Saccharomyces cerevisiae, GI6320764, Length=477, Percent_Identity=26.2054507337526, Blast_Score=165, Evalue=2e-41,
Organism=Drosophila melanogaster, GI18921117, Length=469, Percent_Identity=26.226012793177, Blast_Score=164, Evalue=1e-40,
Organism=Drosophila melanogaster, GI24639277, Length=469, Percent_Identity=26.226012793177, Blast_Score=164, Evalue=1e-40,
Organism=Drosophila melanogaster, GI24639275, Length=469, Percent_Identity=26.226012793177, Blast_Score=164, Evalue=1e-40,
Organism=Drosophila melanogaster, GI24653753, Length=454, Percent_Identity=22.0264317180617, Blast_Score=100, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR016164
- InterPro:   IPR016168
- InterPro:   IPR004113
- InterPro:   IPR004490
- InterPro:   IPR006094
- InterPro:   IPR016171 [H]

Pfam domain/function: PF02913 FAD-oxidase_C; PF01565 FAD_binding_4 [H]

EC number: 1.1.3.15

Molecular weight: Translated: 50459; Mature: 50459

Theoretical pI: Translated: 7.18; Mature: 7.18

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEKQHIQYFKNLVGGEDFFTDLAHLNAYCYDATKERHLPSGVIFPKNEQEISQILKYCN
CCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCCHHHHHHHHHHHC
EHRLIVVPRGAGSGFTGGALSVSGGLVLSVEKHLDKILEIDTKNLIARVEPGVINKHFQN
CCCEEEEECCCCCCCCCCEEEECCCEEEEHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHH
EVEKLNLFYPPDPASENQSTLGGNAAENAGGMRAAKYGITKDYVMAMRVVLANGEIIRAG
HHHHEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHCCCCEEECH
KKTIKDVAGFNVAGLMIASEGCLGVISEITLKLLAKPLLKQSAMGVFNHIEDAMSAVYKT
HHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MSSGVTPVAMEFLDNLSIKAVEERFSKGLPKDAGAILITQVDGVVKEQIAWQLNEIEKHF
HHCCCCHHHHHHHHCCCHHHHHHHHHCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHH
KANGCVGFKIAQNEQEEQDLWFSRRNASQSISVYGKKKLNEDVTVPRASLPSLLQEVAKI
HCCCEEEEEECCCCCHHHHHHHHHCCCCCCEEEHHHHHCCCCCCCCCHHHHHHHHHHHHH
SQKYGFKIPCFGHTGDGNVHVNIMLEDPKRDLEKGHQAMEEIFQAAISLEGTLSGEHGIG
HHHCCCCCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
LSKAKFMPLAFNHSEMELFRNIKKALDPNNILNPFKMGL
CCCCCCEEEEECCHHHHHHHHHHHHCCCCCCCCHHHCCC
>Mature Secondary Structure
MLEKQHIQYFKNLVGGEDFFTDLAHLNAYCYDATKERHLPSGVIFPKNEQEISQILKYCN
CCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCCHHHHHHHHHHHC
EHRLIVVPRGAGSGFTGGALSVSGGLVLSVEKHLDKILEIDTKNLIARVEPGVINKHFQN
CCCEEEEECCCCCCCCCCEEEECCCEEEEHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHH
EVEKLNLFYPPDPASENQSTLGGNAAENAGGMRAAKYGITKDYVMAMRVVLANGEIIRAG
HHHHEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHCCCCEEECH
KKTIKDVAGFNVAGLMIASEGCLGVISEITLKLLAKPLLKQSAMGVFNHIEDAMSAVYKT
HHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MSSGVTPVAMEFLDNLSIKAVEERFSKGLPKDAGAILITQVDGVVKEQIAWQLNEIEKHF
HHCCCCHHHHHHHHCCCHHHHHHHHHCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHH
KANGCVGFKIAQNEQEEQDLWFSRRNASQSISVYGKKKLNEDVTVPRASLPSLLQEVAKI
HCCCEEEEEECCCCCHHHHHHHHHCCCCCCEEEHHHHHCCCCCCCCCHHHHHHHHHHHHH
SQKYGFKIPCFGHTGDGNVHVNIMLEDPKRDLEKGHQAMEEIFQAAISLEGTLSGEHGIG
HHHCCCCCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
LSKAKFMPLAFNHSEMELFRNIKKALDPNNILNPFKMGL
CCCCCCEEEEECCHHHHHHHHHHHHCCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (S)-2-hydroxy acid; O2

Specific reaction: (S)-2-hydroxy acid + O2 = 2-oxo acid + H2O2

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]