Click here to switch to the map view.

The map label for this gene is ddl

Identifier: 188527419

GI number: 188527419

Start: 600835

End: 601878

Strand: Reverse

Name: ddl

Synonym: HPSH_03150

Alternate gene names: 188527419

Gene position: 601878-600835 (Counterclockwise)

Preceding gene: 188527420

Following gene: 188527418

Centisome position: 37.42

GC content: 36.02

Gene sequence:

>1044_bases
GTGGAATTTTGCGTTTTATTTGGTGGGGCGAGTTTTGAACATGAAATCAGCATTGTGAGCGCGATCGCGCTTAAAGAAGC
GCTAAAGGGTAGGATTAAATATTTTATTTTTTTAGATGAAAACCATCATTTTTATTTGATTGAAGAATCTAACATGCATT
CAAAATACTTCGCTCAGATCAAAGAAAAAAAATTACCCCCCCTAATCCTTACGTGCAATGGCTTGCTTAAAAACTCGTTT
TTAGGCGCTAAGATTATAGAATTGCCCTTAGTGATTAATCTTGTGCATGGGGGCGATGGCGAAGACGGGAAATTAGCGAG
CTTGTTAGAATTTTATCGTATCGCTTTTATAGGCCCTGGGATTGAAGCGAGCGTGTTGAGTTATAACAAATATTTAACCA
AGCTTTACGCCAAAGACTTAGGGGTGAAGGCTTTAGATCATGTTCTTTTGAATGAAAAAAACCGCGCTAACGCTCTAAAT
TTGATTGGCTTTAATTTTCCTTTCATAATCAAGCCTAGCAATGCCGGGAGCTCTTTAGGGGTGAGCGTTGTGAAAGAAGA
AAAAGAATTGATTTACGCTTTAGACGGTGCATTTGAATATTCTAAAGAGATCTTAATAGAGCCTTTCATTCAGGGAGTGA
AAGAATACAATTTAGCCGGTTGCAAGATCAAAAAGGATTTCTGTTTTTCCTATGTTGAAGAGCCTAACAAACAGGAATTT
TTAGATTTCAAACAAAAATATTTGGATTTTTCACGCAATAAAGCCCCTAAAGCGAATCTTTCTAACGCCCTAGAAGAACA
ATTGAAAGAAAATTTTAAAAAACTCTATAACGATTTGTTTGATGGCGCGCTCATCCGTTGCGATTTTTTTGTCATAGAAA
ATGAAGTGTATCTTAATGAGATCAACCCCATTCCTGGCAGTTTGGCCAATTATTTGTTTGATGATTTTAAAACAACGCTA
GAAAATTTAGCGCAATCATTACCCAAAACCCCTAAAATCCAAATCAAAAACTCTTATTTGTTGCAAATCCAAAAGAATAA
GTAA

Upstream 100 bases:

>100_bases
GATTTTTTAACATTAAACTTTAATTTTAAGAAAATTCAAAAGCCTTTTTTTAGGTAAATATAGATAGACTTTATTGCAAT
CGTTTTTAGGGAGTTTGATC

Downstream 100 bases:

>100_bases
TGGCCAAACGCAGTATCGCTTATTTGGATAGCGTTTTTGACATTTCCTACACTTTTATAGACCACCATAGCCCTTTAAAC
GCCTTGTTTTTGCATGGTTG

Product: D-alanyl-alanine synthetase A

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase [H]

Number of amino acids: Translated: 347; Mature: 347

Protein sequence:

>347_residues
MEFCVLFGGASFEHEISIVSAIALKEALKGRIKYFIFLDENHHFYLIEESNMHSKYFAQIKEKKLPPLILTCNGLLKNSF
LGAKIIELPLVINLVHGGDGEDGKLASLLEFYRIAFIGPGIEASVLSYNKYLTKLYAKDLGVKALDHVLLNEKNRANALN
LIGFNFPFIIKPSNAGSSLGVSVVKEEKELIYALDGAFEYSKEILIEPFIQGVKEYNLAGCKIKKDFCFSYVEEPNKQEF
LDFKQKYLDFSRNKAPKANLSNALEEQLKENFKKLYNDLFDGALIRCDFFVIENEVYLNEINPIPGSLANYLFDDFKTTL
ENLAQSLPKTPKIQIKNSYLLQIQKNK

Sequences:

>Translated_347_residues
MEFCVLFGGASFEHEISIVSAIALKEALKGRIKYFIFLDENHHFYLIEESNMHSKYFAQIKEKKLPPLILTCNGLLKNSF
LGAKIIELPLVINLVHGGDGEDGKLASLLEFYRIAFIGPGIEASVLSYNKYLTKLYAKDLGVKALDHVLLNEKNRANALN
LIGFNFPFIIKPSNAGSSLGVSVVKEEKELIYALDGAFEYSKEILIEPFIQGVKEYNLAGCKIKKDFCFSYVEEPNKQEF
LDFKQKYLDFSRNKAPKANLSNALEEQLKENFKKLYNDLFDGALIRCDFFVIENEVYLNEINPIPGSLANYLFDDFKTTL
ENLAQSLPKTPKIQIKNSYLLQIQKNK
>Mature_347_residues
MEFCVLFGGASFEHEISIVSAIALKEALKGRIKYFIFLDENHHFYLIEESNMHSKYFAQIKEKKLPPLILTCNGLLKNSF
LGAKIIELPLVINLVHGGDGEDGKLASLLEFYRIAFIGPGIEASVLSYNKYLTKLYAKDLGVKALDHVLLNEKNRANALN
LIGFNFPFIIKPSNAGSSLGVSVVKEEKELIYALDGAFEYSKEILIEPFIQGVKEYNLAGCKIKKDFCFSYVEEPNKQEF
LDFKQKYLDFSRNKAPKANLSNALEEQLKENFKKLYNDLFDGALIRCDFFVIENEVYLNEINPIPGSLANYLFDDFKTTL
ENLAQSLPKTPKIQIKNSYLLQIQKNK

Specific function: Cell wall formation [H]

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786579, Length=323, Percent_Identity=27.2445820433437, Blast_Score=111, Evalue=5e-26,
Organism=Escherichia coli, GI1786280, Length=225, Percent_Identity=30.2222222222222, Blast_Score=87, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N [H]

EC number: =6.3.2.4 [H]

Molecular weight: Translated: 39458; Mature: 39458

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS50975 ATP_GRASP ; PS00843 DALA_DALA_LIGASE_1 ; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFCVLFGGASFEHEISIVSAIALKEALKGRIKYFIFLDENHHFYLIEESNMHSKYFAQI
CCEEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEECCCEEEEEECCCCCHHHHHHH
KEKKLPPLILTCNGLLKNSFLGAKIIELPLVINLVHGGDGEDGKLASLLEFYRIAFIGPG
HHCCCCCEEEECCHHHHCCCCCHHHEEEEEEEEEEECCCCCCCHHHHHHHHHHHHHCCCC
IEASVLSYNKYLTKLYAKDLGVKALDHVLLNEKNRANALNLIGFNFPFIIKPSNAGSSLG
CCHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCEEEEEECCCCEEEEECCCCCCCC
VSVVKEEKELIYALDGAFEYSKEILIEPFIQGVKEYNLAGCKIKKDFCFSYVEEPNKQEF
HHHEEHHHHEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHCCCCHHHH
LDFKQKYLDFSRNKAPKANLSNALEEQLKENFKKLYNDLFDGALIRCDFFVIENEVYLNE
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEECEEEEEC
INPIPGSLANYLFDDFKTTLENLAQSLPKTPKIQIKNSYLLQIQKNK
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCEEEEEEECCC
>Mature Secondary Structure
MEFCVLFGGASFEHEISIVSAIALKEALKGRIKYFIFLDENHHFYLIEESNMHSKYFAQI
CCEEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEECCCEEEEEECCCCCHHHHHHH
KEKKLPPLILTCNGLLKNSFLGAKIIELPLVINLVHGGDGEDGKLASLLEFYRIAFIGPG
HHCCCCCEEEECCHHHHCCCCCHHHEEEEEEEEEEECCCCCCCHHHHHHHHHHHHHCCCC
IEASVLSYNKYLTKLYAKDLGVKALDHVLLNEKNRANALNLIGFNFPFIIKPSNAGSSLG
CCHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCEEEEEECCCCEEEEECCCCCCCC
VSVVKEEKELIYALDGAFEYSKEILIEPFIQGVKEYNLAGCKIKKDFCFSYVEEPNKQEF
HHHEEHHHHEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHCCCCHHHH
LDFKQKYLDFSRNKAPKANLSNALEEQLKENFKKLYNDLFDGALIRCDFFVIENEVYLNE
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEECEEEEEC
INPIPGSLANYLFDDFKTTLENLAQSLPKTPKIQIKNSYLLQIQKNK
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA