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The map label for this gene is 188527400

Identifier: 188527400

GI number: 188527400

Start: 583519

End: 584397

Strand: Reverse

Name: 188527400

Synonym: HPSH_03045

Alternate gene names: NA

Gene position: 584397-583519 (Counterclockwise)

Preceding gene: 188527401

Following gene: 188527399

Centisome position: 36.33

GC content: 41.98

Gene sequence:

>879_bases
ATGATTTATGCAAGCGTCCTCCAGCATGCTTATTGCGGCTCTAGAAAAAAAACCATAGAGCATACAGCAAACTTGCTTGA
ACAAGCGCTAAAAAAACACCCTAAAACCAATTTAGTGGTGTTGCAAGAATTAAACCCTTATAGTTATTTTTGCCAGAGCG
AAAACCCTAAATTTTTTGATTTGGGCGAATATTTTGAAGAAGATAAGGCTTTTTTTAGCGCTTTAGCCCAAAAATTTCAA
GTGGTGCTGATCGCTTCTTTATTTGAAAAGCGCGCTAAAGGGCTGTATCACAATAGCGCTGTTGTGTTTGAAAAAGACGG
ATCAATCGCTGGGGTGTATCGCAAAATGCACATTCCTGATGACCCGGGGTTTTATGAAAAATTTTATTTCACGCCGGGGG
ATTTGGGCTTTGAGCCTATTGTTACAAGCGTGGGCAAATTAGGGCTTATGGTGTGTTGGGATCAGTGGTATCCTGAGGCA
GCAAGGATTATGGCTTTAAAAGGGGCAGAAATTTTAATCTATCCTAGCGCGATAGGGTTTTTAGAAGAAGACTCTAATGA
AGAAAAAAAACGCCAGCAAAACGCATGGGAGACCATCCAAAGAGGGCATGCGATCGCTAATGGCTTGCCTTTGATTGCGA
CTAACAGAGTGGGTGTAGAGTTAGATCCTAGCGGCGCGATTAAGGGGGGCATCACTTTTTTTGGCTCTAGTTTTGTGGTG
GGAGCTTTGGGCGAATTTTTAGCTAAAGCGAGCGATAAAGAAGAGATTTTGTATGCGGAAATTGATTTAGAACGCACCGA
AGAAGTGCGCCGAATGTGGCCGTTTTTGAGAGACAGGCGCATTGATTTTTATAACGATTTGTTGAAACGCTATATTTAA

Upstream 100 bases:

>100_bases
GGGCTTTGAAGAAGAAGATGAAGAGTATGGGGATTATAAGAATGTCTATGACGATGACGATTATGAAGACTATAGCTCTG
ATTATGAAGAAGAGTGAAAA

Downstream 100 bases:

>100_bases
TCAGTCAATCAATTTAAAATTTAAGGTTAGAAAGGATTAAACATGGTAGGTGTGGTTTTTTGCGCTAAACAAGCGCAAAA
ATTCTATCATTTTTGCGCGG

Product: carbon-nitrogen hydrolase

Products: NA

Alternate protein names: D-N-alpha-carbamilase [H]

Number of amino acids: Translated: 292; Mature: 292

Protein sequence:

>292_residues
MIYASVLQHAYCGSRKKTIEHTANLLEQALKKHPKTNLVVLQELNPYSYFCQSENPKFFDLGEYFEEDKAFFSALAQKFQ
VVLIASLFEKRAKGLYHNSAVVFEKDGSIAGVYRKMHIPDDPGFYEKFYFTPGDLGFEPIVTSVGKLGLMVCWDQWYPEA
ARIMALKGAEILIYPSAIGFLEEDSNEEKKRQQNAWETIQRGHAIANGLPLIATNRVGVELDPSGAIKGGITFFGSSFVV
GALGEFLAKASDKEEILYAEIDLERTEEVRRMWPFLRDRRIDFYNDLLKRYI

Sequences:

>Translated_292_residues
MIYASVLQHAYCGSRKKTIEHTANLLEQALKKHPKTNLVVLQELNPYSYFCQSENPKFFDLGEYFEEDKAFFSALAQKFQ
VVLIASLFEKRAKGLYHNSAVVFEKDGSIAGVYRKMHIPDDPGFYEKFYFTPGDLGFEPIVTSVGKLGLMVCWDQWYPEA
ARIMALKGAEILIYPSAIGFLEEDSNEEKKRQQNAWETIQRGHAIANGLPLIATNRVGVELDPSGAIKGGITFFGSSFVV
GALGEFLAKASDKEEILYAEIDLERTEEVRRMWPFLRDRRIDFYNDLLKRYI
>Mature_292_residues
MIYASVLQHAYCGSRKKTIEHTANLLEQALKKHPKTNLVVLQELNPYSYFCQSENPKFFDLGEYFEEDKAFFSALAQKFQ
VVLIASLFEKRAKGLYHNSAVVFEKDGSIAGVYRKMHIPDDPGFYEKFYFTPGDLGFEPIVTSVGKLGLMVCWDQWYPEA
ARIMALKGAEILIYPSAIGFLEEDSNEEKKRQQNAWETIQRGHAIANGLPLIATNRVGVELDPSGAIKGGITFFGSSFVV
GALGEFLAKASDKEEILYAEIDLERTEEVRRMWPFLRDRRIDFYNDLLKRYI

Specific function: The enzyme catalyzes the hydrolysis of N-carbamoyl-D- amino acids to the corresponding which are useful intermediates in the preparation of beta-lactam antibiotics. Industrial production of beta-lactam antibiotics is now being developed using this enzyme

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI9910460, Length=224, Percent_Identity=31.6964285714286, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI297632350, Length=196, Percent_Identity=30.6122448979592, Blast_Score=99, Evalue=6e-21,
Organism=Homo sapiens, GI5031947, Length=196, Percent_Identity=30.6122448979592, Blast_Score=99, Evalue=6e-21,
Organism=Homo sapiens, GI297632348, Length=196, Percent_Identity=30.6122448979592, Blast_Score=99, Evalue=7e-21,
Organism=Homo sapiens, GI7706509, Length=265, Percent_Identity=28.3018867924528, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17533173, Length=264, Percent_Identity=30.6818181818182, Blast_Score=107, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI17556280, Length=296, Percent_Identity=26.6891891891892, Blast_Score=93, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6323383, Length=285, Percent_Identity=29.8245614035088, Blast_Score=125, Evalue=7e-30,
Organism=Drosophila melanogaster, GI21358471, Length=270, Percent_Identity=30.3703703703704, Blast_Score=105, Evalue=3e-23,
Organism=Drosophila melanogaster, GI21355835, Length=285, Percent_Identity=27.719298245614, Blast_Score=87, Evalue=9e-18,
Organism=Drosophila melanogaster, GI17933642, Length=192, Percent_Identity=29.1666666666667, Blast_Score=84, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: =3.5.1.77 [H]

Molecular weight: Translated: 33309; Mature: 33309

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYASVLQHAYCGSRKKTIEHTANLLEQALKKHPKTNLVVLQELNPYSYFCQSENPKFFD
CCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCEEEECCCCCCEEE
LGEYFEEDKAFFSALAQKFQVVLIASLFEKRAKGLYHNSAVVFEKDGSIAGVYRKMHIPD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCEEEHEEEECCCC
DPGFYEKFYFTPGDLGFEPIVTSVGKLGLMVCWDQWYPEAARIMALKGAEILIYPSAIGF
CCCCCCEEEECCCCCCHHHHHHHHHHHHHEEEHHHCCCCHHHEEEECCCEEEEEECHHCC
LEEDSNEEKKRQQNAWETIQRGHAIANGLPLIATNRVGVELDPSGAIKGGITFFGSSFVV
CCCCCCHHHHHHHHHHHHHHHCHHHHCCCCEEEECCCCEEECCCCCCCCCHHHHCHHHHH
GALGEFLAKASDKEEILYAEIDLERTEEVRRMWPFLRDRRIDFYNDLLKRYI
HHHHHHHHCCCCCCCEEEEEECHHHHHHHHHHHHHHHHCCCHHHHHHHHHCC
>Mature Secondary Structure
MIYASVLQHAYCGSRKKTIEHTANLLEQALKKHPKTNLVVLQELNPYSYFCQSENPKFFD
CCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCEEEECCCCCCEEE
LGEYFEEDKAFFSALAQKFQVVLIASLFEKRAKGLYHNSAVVFEKDGSIAGVYRKMHIPD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCEEEHEEEECCCC
DPGFYEKFYFTPGDLGFEPIVTSVGKLGLMVCWDQWYPEAARIMALKGAEILIYPSAIGF
CCCCCCEEEECCCCCCHHHHHHHHHHHHHEEEHHHCCCCHHHEEEECCCEEEEEECHHCC
LEEDSNEEKKRQQNAWETIQRGHAIANGLPLIATNRVGVELDPSGAIKGGITFFGSSFVV
CCCCCCHHHHHHHHHHHHHHHCHHHHCCCCEEEECCCCEEECCCCCCCCCHHHHCHHHHH
GALGEFLAKASDKEEILYAEIDLERTEEVRRMWPFLRDRRIDFYNDLLKRYI
HHHHHHHHCCCCCCCEEEEEECHHHHHHHHHHHHHHHHCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9648217; 10903946 [H]