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The map label for this gene is gltX

Identifier: 188527262

GI number: 188527262

Start: 449951

End: 451342

Strand: Reverse

Name: gltX

Synonym: HPSH_02345

Alternate gene names: 188527262

Gene position: 451342-449951 (Counterclockwise)

Preceding gene: 188527271

Following gene: 188527258

Centisome position: 28.06

GC content: 39.37

Gene sequence:

>1392_bases
ATGAGTTTGATCGTTACGCGCTTCGCTCCATCGCCCACCGGCTATCTCCACATAGGAGGCTTAAGAACAGCCATTTTCAA
TTATCTTTTTGCACGAGCCAATCAAGGAAAATTTTTTTTACGCATTGAAGACACGGATTTGAGCCGTAACTCTATAGAAG
CGGCTAACGCCATTATAGAGGCTTTCAAATGGGTAGGGCTAGAACACGATGGAGAAATCCTCTACCAATCCAAACGCTTT
GGAATTTATAAAGAATATATCCAGAAACTCTTAGATGAAGACAAAGCCTATTACTGCTACATGAGTAAAGATGAGTTGGA
CGCTTTGAGAGAAGAACAAAAGGCCAGGAAAGAAACCCCACGCTATGACAATCGTTATCGTGATTTTAAAGGCACGCCCC
CTAAAGGCATAGAGCCTGTGGTAAGGATTAAAGTCCCGCAAAATGAAATCATTGGTTTTAATGACGGGGTTAAAGGCGAA
GTGAAAGTGAATACTAACGAATTAGACGATTTTATTATCGCTAGGAGCGATGGAACGCCCACTTATAACTTTGTGGTTAC
CATTGATGACGCTTTAATGGGGATTACTGACGTGATTAGAGGCGATGATCACCTTTCTAACACCCCTAAACAAATCGTTC
TCTATAAGGCTTTAAATTTTAAAATCCCTAATTTTTTCCATGTGCCGATGATTTTGAATGAAGAAGGGCAAAAATTAAGC
AAACGCCATGGGGCCACTAATGTGATGGACTATCAAGAAATGGGCTATCTTAAGGAAGCGTTAGTGAATTTTTTAGCGCG
TTTGGGGTGGAGCTGTCACGATAAAGAGGTTTTTAGCATGCAAGAATTACTAAAATGGTTTGACCCTAAAGATTTAAATT
CTTCGCCCAGTTGCTTTAGCTGGCACAAGCTTAATTGGCTCAACGCTCATTATTTAAAAAACCAAAGCGCGCAAAGATTG
TTAGAGCTTTTAAAGCCTTTTAATTTTAGCGATCTCTCGCATTTAAACCCCACCCAATTGGATCGCTTGTTAGACGCTCT
CAAAGAAAGATCTCAAACCTTAAAAGAATTAGCCCTTAAAATAGATGAGGTTTTAATCGCTCCTGTAGAGTATGAAGAAA
AGGTTTTTAAAAAGCTCAATCAAGCGCTCGTTATGCCCTTGTTAGAAAAGTTTAAACTAGAATTGAATAAAGCCAATTTC
AACGATGAAAGCGTGCTAGAAAACGCCATGCACAAAATCATTGAAGAAGAAAAGATTAAAGCGGGTAGTTTTATGCAGCC
TTTAAGATTAGCCCTTTTGGGTAAGGGGGGCGGGATAGGCCTTAAAGAAGCGCTTTTCATTTTAGGCAAAACAGAGAGCG
TTAAAAGAATAGAAGAATTTTTGAAAAACTAA

Upstream 100 bases:

>100_bases
AATAATCTCAATCATACACTAATTTTAATAGAAAAAAGTGAAACCATTTTTAAGCCATTTTTGAATACAATAAGGGTATT
TTATTTTTAAGGTTAGAATA

Downstream 100 bases:

>100_bases
AAGATTGGCTCTCTAAAATGGGCGCATAAAGTTTGCATTTTTGAAAAATAAGAGGGTTTTAAGAGAAAAATAAAAGTATT
TCTAAAATTTTTTGCCCTGA

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase 1; GluRS 1 [H]

Number of amino acids: Translated: 463; Mature: 462

Protein sequence:

>463_residues
MSLIVTRFAPSPTGYLHIGGLRTAIFNYLFARANQGKFFLRIEDTDLSRNSIEAANAIIEAFKWVGLEHDGEILYQSKRF
GIYKEYIQKLLDEDKAYYCYMSKDELDALREEQKARKETPRYDNRYRDFKGTPPKGIEPVVRIKVPQNEIIGFNDGVKGE
VKVNTNELDDFIIARSDGTPTYNFVVTIDDALMGITDVIRGDDHLSNTPKQIVLYKALNFKIPNFFHVPMILNEEGQKLS
KRHGATNVMDYQEMGYLKEALVNFLARLGWSCHDKEVFSMQELLKWFDPKDLNSSPSCFSWHKLNWLNAHYLKNQSAQRL
LELLKPFNFSDLSHLNPTQLDRLLDALKERSQTLKELALKIDEVLIAPVEYEEKVFKKLNQALVMPLLEKFKLELNKANF
NDESVLENAMHKIIEEEKIKAGSFMQPLRLALLGKGGGIGLKEALFILGKTESVKRIEEFLKN

Sequences:

>Translated_463_residues
MSLIVTRFAPSPTGYLHIGGLRTAIFNYLFARANQGKFFLRIEDTDLSRNSIEAANAIIEAFKWVGLEHDGEILYQSKRF
GIYKEYIQKLLDEDKAYYCYMSKDELDALREEQKARKETPRYDNRYRDFKGTPPKGIEPVVRIKVPQNEIIGFNDGVKGE
VKVNTNELDDFIIARSDGTPTYNFVVTIDDALMGITDVIRGDDHLSNTPKQIVLYKALNFKIPNFFHVPMILNEEGQKLS
KRHGATNVMDYQEMGYLKEALVNFLARLGWSCHDKEVFSMQELLKWFDPKDLNSSPSCFSWHKLNWLNAHYLKNQSAQRL
LELLKPFNFSDLSHLNPTQLDRLLDALKERSQTLKELALKIDEVLIAPVEYEEKVFKKLNQALVMPLLEKFKLELNKANF
NDESVLENAMHKIIEEEKIKAGSFMQPLRLALLGKGGGIGLKEALFILGKTESVKRIEEFLKN
>Mature_462_residues
SLIVTRFAPSPTGYLHIGGLRTAIFNYLFARANQGKFFLRIEDTDLSRNSIEAANAIIEAFKWVGLEHDGEILYQSKRFG
IYKEYIQKLLDEDKAYYCYMSKDELDALREEQKARKETPRYDNRYRDFKGTPPKGIEPVVRIKVPQNEIIGFNDGVKGEV
KVNTNELDDFIIARSDGTPTYNFVVTIDDALMGITDVIRGDDHLSNTPKQIVLYKALNFKIPNFFHVPMILNEEGQKLSK
RHGATNVMDYQEMGYLKEALVNFLARLGWSCHDKEVFSMQELLKWFDPKDLNSSPSCFSWHKLNWLNAHYLKNQSAQRLL
ELLKPFNFSDLSHLNPTQLDRLLDALKERSQTLKELALKIDEVLIAPVEYEEKVFKKLNQALVMPLLEKFKLELNKANFN
DESVLENAMHKIIEEEKIKAGSFMQPLRLALLGKGGGIGLKEALFILGKTESVKRIEEFLKN

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) [H]

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=494, Percent_Identity=30.1619433198381, Blast_Score=216, Evalue=3e-56,
Organism=Homo sapiens, GI62241042, Length=436, Percent_Identity=22.4770642201835, Blast_Score=70, Evalue=6e-12,
Organism=Escherichia coli, GI1788743, Length=459, Percent_Identity=42.483660130719, Blast_Score=363, Evalue=1e-101,
Organism=Escherichia coli, GI226510925, Length=242, Percent_Identity=35.9504132231405, Blast_Score=162, Evalue=4e-41,
Organism=Escherichia coli, GI1786895, Length=213, Percent_Identity=25.8215962441315, Blast_Score=69, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI71993394, Length=302, Percent_Identity=39.4039735099338, Blast_Score=191, Evalue=8e-49,
Organism=Caenorhabditis elegans, GI17511007, Length=258, Percent_Identity=23.6434108527132, Blast_Score=76, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6324540, Length=489, Percent_Identity=33.9468302658487, Blast_Score=223, Evalue=4e-59,
Organism=Saccharomyces cerevisiae, GI6321192, Length=218, Percent_Identity=28.8990825688073, Blast_Score=87, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6324742, Length=128, Percent_Identity=29.6875, Blast_Score=66, Evalue=9e-12,
Organism=Drosophila melanogaster, GI24665321, Length=491, Percent_Identity=33.1975560081466, Blast_Score=237, Evalue=1e-62,
Organism=Drosophila melanogaster, GI24649466, Length=250, Percent_Identity=26, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI17864488, Length=363, Percent_Identity=24.2424242424242, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: =6.1.1.17 [H]

Molecular weight: Translated: 53325; Mature: 53194

Theoretical pI: Translated: 7.07; Mature: 7.07

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLIVTRFAPSPTGYLHIGGLRTAIFNYLFARANQGKFFLRIEDTDLSRNSIEAANAIIE
CCEEEEECCCCCCCEEEECHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHH
AFKWVGLEHDGEILYQSKRFGIYKEYIQKLLDEDKAYYCYMSKDELDALREEQKARKETP
HHHHCCCCCCCCEEEECHHCCHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHCCC
RYDNRYRDFKGTPPKGIEPVVRIKVPQNEIIGFNDGVKGEVKVNTNELDDFIIARSDGTP
CCCCCCCCCCCCCCCCCCCEEEEECCCCCEECCCCCCCEEEEECCCCCCCEEEEECCCCC
TYNFVVTIDDALMGITDVIRGDDHLSNTPKQIVLYKALNFKIPNFFHVPMILNEEGQKLS
EEEEEEEECHHHHHHHHHHCCCCCCCCCCHHEEEEEECCCCCCCEEECCEEECCCHHHHH
KRHGATNVMDYQEMGYLKEALVNFLARLGWSCHDKEVFSMQELLKWFDPKDLNSSPSCFS
HHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCE
WHKLNWLNAHYLKNQSAQRLLELLKPFNFSDLSHLNPTQLDRLLDALKERSQTLKELALK
ECCCCCCHHHHHCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
IDEVLIAPVEYEEKVFKKLNQALVMPLLEKFKLELNKANFNDESVLENAMHKIIEEEKIK
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHC
AGSFMQPLRLALLGKGGGIGLKEALFILGKTESVKRIEEFLKN
CCCHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHCC
>Mature Secondary Structure 
SLIVTRFAPSPTGYLHIGGLRTAIFNYLFARANQGKFFLRIEDTDLSRNSIEAANAIIE
CEEEEECCCCCCCEEEECHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHH
AFKWVGLEHDGEILYQSKRFGIYKEYIQKLLDEDKAYYCYMSKDELDALREEQKARKETP
HHHHCCCCCCCCEEEECHHCCHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHCCC
RYDNRYRDFKGTPPKGIEPVVRIKVPQNEIIGFNDGVKGEVKVNTNELDDFIIARSDGTP
CCCCCCCCCCCCCCCCCCCEEEEECCCCCEECCCCCCCEEEEECCCCCCCEEEEECCCCC
TYNFVVTIDDALMGITDVIRGDDHLSNTPKQIVLYKALNFKIPNFFHVPMILNEEGQKLS
EEEEEEEECHHHHHHHHHHCCCCCCCCCCHHEEEEEECCCCCCCEEECCEEECCCHHHHH
KRHGATNVMDYQEMGYLKEALVNFLARLGWSCHDKEVFSMQELLKWFDPKDLNSSPSCFS
HHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCE
WHKLNWLNAHYLKNQSAQRLLELLKPFNFSDLSHLNPTQLDRLLDALKERSQTLKELALK
ECCCCCCHHHHHCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
IDEVLIAPVEYEEKVFKKLNQALVMPLLEKFKLELNKANFNDESVLENAMHKIIEEEKIK
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHC
AGSFMQPLRLALLGKGGGIGLKEALFILGKTESVKRIEEFLKN
CCCHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA