Click here to switch to the map view.

The map label for this gene is kefBC [H]

Identifier: 188527255

GI number: 188527255

Start: 445277

End: 446527

Strand: Reverse

Name: kefBC [H]

Synonym: HPSH_02310

Alternate gene names: 188527255

Gene position: 446527-445277 (Counterclockwise)

Preceding gene: 188527256

Following gene: 188527254

Centisome position: 27.76

GC content: 36.61

Gene sequence:

>1251_bases
ATGGAAAACAGCACACTTTATATTGTTATTGCTGGCTTATGGCTTGCTGTGGGCTTTGGAATCTTTTTAAAGAAATTAGA
CATGCCTGTTATCATTGGTTACATTTGCACAGGAACGGTCTTAGCGGCTTTTTTTAAAATTAATGATTTTAATTTGTTGT
CTGATATTGGCGAATTTGGTATCGTCTTTTTAATGTTTATGATAGGCATTGAGTTTAATTTTGACAAGCTCAAATCCATC
AAACAAGAAGTGCTGGTTTTTGGGCTTTTACAAGTAGTTTTATGCGCTTTAATCGCTTTTTTATTGGGGTATTTTGTTTT
GGGTCTTTCACCCATTTTTTCCCTTGTTTTAGGCATGGGGCTTTCGCTCTCTTCAACCGCTATTGTGCTGAAATTCTTTG
AAGATTCCAAACAGCTTAGCACGCCCATGGGAAAGAGCGCGGTGGGGATTTTGATTTTCCAAGATATTGCAGCCATTCCC
ATGCTTTTAATTTTGACGATTCTAGGCAGTAAAGATTCTAATGTCAATTTACTCATTCTCAAAACCTTTATTTCAGCAGG
GATTATTTTAGTTCTTTTATTATTGCCTGGAAAAAAAGGGGCTAATCTCATCTTAGAGCAAGCAAAAGACACGCGCTTGC
CTGAAATTTTTATAGGCACGATTTTAGTGATTGTTTGCAGCGCGGCGGGGTTGAGCCATTTTTTTGGGTTTTCTATGTCT
TTAGGAGCGTTCATTGTGGGCATGGCCATTTCTAAATCGCGCTATAAAATCAATGTCCAAGAAGAATTCGCGCAATTAAA
AAACCTCTTCTTAGCCCTTTTTTTCATTACGATAGGGATGCAAATTAATGTGAGTTTCTTTATAGAGAAGTTCTTTGTTG
TCATCTTTTTACTCATTTTAGTGATGGGTTTTAAGACGGCTATCATTTATGCGCTCTTGCGTTTTTTTAGAGACGCTAAA
ACTGCCATAAAAACCGCTCTTTCTTTGGCGCAAATCGGGGAGTTTTCTTTTGTTATCTTTTTAAATTCAGGCTCGCACCA
GCTCTTTAATTTGCAAGAAAAAAAAGGGATTCTTGGTTTTTTGCACCAAAAAAATATCTTAAGTATCGCTCAAAATGACA
TCCACCAACTCCTTATTTTGATGGTGGTCTTTTCTATGTTGGCAACCCCTTTTATTTTAAAATACCTAGAATCTATCGCT
CAATTTATTTTGCACCAAAAGAGCCAAGAAAATGAGCCGGCTAAAAAATAA

Upstream 100 bases:

>100_bases
TATTATTTTATTCAATAGTGTTATTTAATGGCTCAATTCAATAAGATAGTTTCGTTTTTATTTCTTTAAAATAAATCGAT
TCTACTGGGGAAAGGTTTTT

Downstream 100 bases:

>100_bases
TTGCGTAAAAACCAAATGATAAGTTTGCTTGTATTGTCAGTGTTATGTTAAAATACCAGCGTATTGAACTCAAACTATTA
AACAAAGGGGGCTTTTATGA

Product: glutathione-regulated potassium-efflux system protein (kefB)

Products: Proton [Cytoplasm]; K (I) [Periplasm] [C]

Alternate protein names: K(+)/H(+) antiporter [H]

Number of amino acids: Translated: 416; Mature: 416

Protein sequence:

>416_residues
MENSTLYIVIAGLWLAVGFGIFLKKLDMPVIIGYICTGTVLAAFFKINDFNLLSDIGEFGIVFLMFMIGIEFNFDKLKSI
KQEVLVFGLLQVVLCALIAFLLGYFVLGLSPIFSLVLGMGLSLSSTAIVLKFFEDSKQLSTPMGKSAVGILIFQDIAAIP
MLLILTILGSKDSNVNLLILKTFISAGIILVLLLLPGKKGANLILEQAKDTRLPEIFIGTILVIVCSAAGLSHFFGFSMS
LGAFIVGMAISKSRYKINVQEEFAQLKNLFLALFFITIGMQINVSFFIEKFFVVIFLLILVMGFKTAIIYALLRFFRDAK
TAIKTALSLAQIGEFSFVIFLNSGSHQLFNLQEKKGILGFLHQKNILSIAQNDIHQLLILMVVFSMLATPFILKYLESIA
QFILHQKSQENEPAKK

Sequences:

>Translated_416_residues
MENSTLYIVIAGLWLAVGFGIFLKKLDMPVIIGYICTGTVLAAFFKINDFNLLSDIGEFGIVFLMFMIGIEFNFDKLKSI
KQEVLVFGLLQVVLCALIAFLLGYFVLGLSPIFSLVLGMGLSLSSTAIVLKFFEDSKQLSTPMGKSAVGILIFQDIAAIP
MLLILTILGSKDSNVNLLILKTFISAGIILVLLLLPGKKGANLILEQAKDTRLPEIFIGTILVIVCSAAGLSHFFGFSMS
LGAFIVGMAISKSRYKINVQEEFAQLKNLFLALFFITIGMQINVSFFIEKFFVVIFLLILVMGFKTAIIYALLRFFRDAK
TAIKTALSLAQIGEFSFVIFLNSGSHQLFNLQEKKGILGFLHQKNILSIAQNDIHQLLILMVVFSMLATPFILKYLESIA
QFILHQKSQENEPAKK
>Mature_416_residues
MENSTLYIVIAGLWLAVGFGIFLKKLDMPVIIGYICTGTVLAAFFKINDFNLLSDIGEFGIVFLMFMIGIEFNFDKLKSI
KQEVLVFGLLQVVLCALIAFLLGYFVLGLSPIFSLVLGMGLSLSSTAIVLKFFEDSKQLSTPMGKSAVGILIFQDIAAIP
MLLILTILGSKDSNVNLLILKTFISAGIILVLLLLPGKKGANLILEQAKDTRLPEIFIGTILVIVCSAAGLSHFFGFSMS
LGAFIVGMAISKSRYKINVQEEFAQLKNLFLALFFITIGMQINVSFFIEKFFVVIFLLILVMGFKTAIIYALLRFFRDAK
TAIKTALSLAQIGEFSFVIFLNSGSHQLFNLQEKKGILGFLHQKNILSIAQNDIHQLLILMVVFSMLATPFILKYLESIA
QFILHQKSQENEPAKK

Specific function: Transport system that facilitate potassium-efflux, possibly by potassium-proton antiport [H]

COG id: COG0475

COG function: function code P; Kef-type K+ transport systems, membrane components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RCK N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI157388921, Length=359, Percent_Identity=28.4122562674095, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI1786232, Length=395, Percent_Identity=28.8607594936709, Blast_Score=143, Evalue=2e-35,
Organism=Escherichia coli, GI1789749, Length=392, Percent_Identity=26.7857142857143, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1786685, Length=369, Percent_Identity=26.8292682926829, Blast_Score=122, Evalue=6e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006153
- InterPro:   IPR004771
- InterPro:   IPR016040
- InterPro:   IPR003148 [H]

Pfam domain/function: PF00999 Na_H_Exchanger; PF02254 TrkA_N [H]

EC number: NA

Molecular weight: Translated: 46051; Mature: 46051

Theoretical pI: Translated: 9.63; Mature: 9.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENSTLYIVIAGLWLAVGFGIFLKKLDMPVIIGYICTGTVLAAFFKINDFNLLSDIGEFG
CCCCEEEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
IVFLMFMIGIEFNFDKLKSIKQEVLVFGLLQVVLCALIAFLLGYFVLGLSPIFSLVLGMG
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LSLSSTAIVLKFFEDSKQLSTPMGKSAVGILIFQDIAAIPMLLILTILGSKDSNVNLLIL
CCCCCHHHHHHHHHCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHH
KTFISAGIILVLLLLPGKKGANLILEQAKDTRLPEIFIGTILVIVCSAAGLSHFFGFSMS
HHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGAFIVGMAISKSRYKINVQEEFAQLKNLFLALFFITIGMQINVSFFIEKFFVVIFLLIL
HHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
VMGFKTAIIYALLRFFRDAKTAIKTALSLAQIGEFSFVIFLNSGSHQLFNLQEKKGILGF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCHHCCCHHHCCHHHH
LHQKNILSIAQNDIHQLLILMVVFSMLATPFILKYLESIAQFILHQKSQENEPAKK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MENSTLYIVIAGLWLAVGFGIFLKKLDMPVIIGYICTGTVLAAFFKINDFNLLSDIGEFG
CCCCEEEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
IVFLMFMIGIEFNFDKLKSIKQEVLVFGLLQVVLCALIAFLLGYFVLGLSPIFSLVLGMG
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LSLSSTAIVLKFFEDSKQLSTPMGKSAVGILIFQDIAAIPMLLILTILGSKDSNVNLLIL
CCCCCHHHHHHHHHCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHH
KTFISAGIILVLLLLPGKKGANLILEQAKDTRLPEIFIGTILVIVCSAAGLSHFFGFSMS
HHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGAFIVGMAISKSRYKINVQEEFAQLKNLFLALFFITIGMQINVSFFIEKFFVVIFLLIL
HHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
VMGFKTAIIYALLRFFRDAKTAIKTALSLAQIGEFSFVIFLNSGSHQLFNLQEKKGILGF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCHHCCCHHHCCHHHH
LHQKNILSIAQNDIHQLLILMVVFSMLATPFILKYLESIAQFILHQKSQENEPAKK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; K (I) [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + K (I) [Cytoplasm] = Proton [Cytoplasm] + K (I) [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]