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The map label for this gene is ispB [H]

Identifier: 188527047

GI number: 188527047

Start: 235397

End: 236320

Strand: Reverse

Name: ispB [H]

Synonym: HPSH_01250

Alternate gene names: 188527047

Gene position: 236320-235397 (Counterclockwise)

Preceding gene: 188527048

Following gene: 188527046

Centisome position: 14.69

GC content: 36.15

Gene sequence:

>924_bases
ATGCAAGAAAAACAACTCAAAGCCATTCAAAACAAGATCGCTTCTTGGATCAAAGAAATAGAAAGCGCCTTTATAGATGA
ATTGTTTTCTAAGATTGGCCCTTCAAAAATGCTGCGCTCCAAACTCATGCTCGCTTTATTAAATGGAAAAACAGACACTA
TTTTATTGGATAAAGCATTCAATTTGTGCGCGATTGTAGAAATGATACAGACCGCTTCTTTATTGCATGATGATGTGATT
GACAAGGCGACCATGCGCCGAAAACTCCCCAGCATTAACGCTCTTTTTGGTAATTTTAACGCCGTGATGCTTGGGGATGT
GTTTTATTCTAAAGCTTTTTTTGAACTCTCTAAAATGGGTGAATCCATCGCTCAAGCCCTCTCTAATGCGGTTTTAAGGC
TCTCTAGGGGCGAGATTGAAGATGTGTTTGTGGGGGAATGTTTCAATAGCGACAAACAAAAATACTGGCGTATTTTAGAA
GACAAGACCGCTCATTTCATAGAAGCGAGCTTAAAGAGCATGGCGATTCTTTTAAATAAAGACGCAAAAATATATGCGGA
TTTTGGATTAAATTTTGGCATGGCGTTTCAAATCATTGATGATTTATTAGACATCACTCAAGACGCTAAAACTTTAGGTA
AGCCCAATTTCAGCGATTTTAAAGAAGGCAAGACCACTTTACCCTACTTGCTTTTATATGAAAAATTAAATCAGCATGAA
CAAGGGCTTTTAATTTCTTATTTTAAACAAGATAGCCATGAAATCATAGAATGGACTAAGGAAAAATTCAAGCAATATGG
TATCATAGAAGAAACCCTTAAAACCGCTCAAGTTTATTCTAAAAAGGCCCTTGAAGCCATTAAAAAGGAAAACAATTTGA
TTTTAGAAAAACTAGCGCAAGATGTCATTTACAGGACTTTTTAA

Upstream 100 bases:

>100_bases
GTCGCTTGTGTTTAAAGACTTTAAAGAAGATAAAGCCGCTTTAAGGCTTTTAGCCCTTATGCCCCTTGTTTTTTCTCCTA
AAGAGCCTTAAGGAATTTGC

Downstream 100 bases:

>100_bases
TGGAGTTAGAAACTCATTTGTCAAAATATTTCACCCTAGCCTTTACGCATAAAAGCATGGGCTTAGAAATGCGAGAAAAA
CTCGCTATCAATTCGAGCGC

Product: putative octaprenyl-diphosphate synthase

Products: NA

Alternate protein names: All-trans-octaprenyl-diphosphate synthase; Octaprenyl pyrophosphate synthase; OPP synthase [H]

Number of amino acids: Translated: 307; Mature: 307

Protein sequence:

>307_residues
MQEKQLKAIQNKIASWIKEIESAFIDELFSKIGPSKMLRSKLMLALLNGKTDTILLDKAFNLCAIVEMIQTASLLHDDVI
DKATMRRKLPSINALFGNFNAVMLGDVFYSKAFFELSKMGESIAQALSNAVLRLSRGEIEDVFVGECFNSDKQKYWRILE
DKTAHFIEASLKSMAILLNKDAKIYADFGLNFGMAFQIIDDLLDITQDAKTLGKPNFSDFKEGKTTLPYLLLYEKLNQHE
QGLLISYFKQDSHEIIEWTKEKFKQYGIIEETLKTAQVYSKKALEAIKKENNLILEKLAQDVIYRTF

Sequences:

>Translated_307_residues
MQEKQLKAIQNKIASWIKEIESAFIDELFSKIGPSKMLRSKLMLALLNGKTDTILLDKAFNLCAIVEMIQTASLLHDDVI
DKATMRRKLPSINALFGNFNAVMLGDVFYSKAFFELSKMGESIAQALSNAVLRLSRGEIEDVFVGECFNSDKQKYWRILE
DKTAHFIEASLKSMAILLNKDAKIYADFGLNFGMAFQIIDDLLDITQDAKTLGKPNFSDFKEGKTTLPYLLLYEKLNQHE
QGLLISYFKQDSHEIIEWTKEKFKQYGIIEETLKTAQVYSKKALEAIKKENNLILEKLAQDVIYRTF
>Mature_307_residues
MQEKQLKAIQNKIASWIKEIESAFIDELFSKIGPSKMLRSKLMLALLNGKTDTILLDKAFNLCAIVEMIQTASLLHDDVI
DKATMRRKLPSINALFGNFNAVMLGDVFYSKAFFELSKMGESIAQALSNAVLRLSRGEIEDVFVGECFNSDKQKYWRILE
DKTAHFIEASLKSMAILLNKDAKIYADFGLNFGMAFQIIDDLLDITQDAKTLGKPNFSDFKEGKTTLPYLLLYEKLNQHE
QGLLISYFKQDSHEIIEWTKEKFKQYGIIEETLKTAQVYSKKALEAIKKENNLILEKLAQDVIYRTF

Specific function: Supplies octaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinones ubiquinone and menaquinone [H]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=229, Percent_Identity=31.8777292576419, Blast_Score=109, Evalue=3e-24,
Organism=Escherichia coli, GI1789578, Length=255, Percent_Identity=31.3725490196078, Blast_Score=131, Evalue=4e-32,
Organism=Escherichia coli, GI1786623, Length=240, Percent_Identity=28.3333333333333, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17505681, Length=244, Percent_Identity=33.6065573770492, Blast_Score=108, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6319475, Length=282, Percent_Identity=31.2056737588652, Blast_Score=106, Evalue=4e-24,
Organism=Drosophila melanogaster, GI24651612, Length=203, Percent_Identity=33.4975369458128, Blast_Score=105, Evalue=3e-23,
Organism=Drosophila melanogaster, GI281365769, Length=265, Percent_Identity=24.9056603773585, Blast_Score=66, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24660002, Length=265, Percent_Identity=24.9056603773585, Blast_Score=66, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.90 [H]

Molecular weight: Translated: 35126; Mature: 35126

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: PS00444 POLYPRENYL_SYNTHET_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQEKQLKAIQNKIASWIKEIESAFIDELFSKIGPSKMLRSKLMLALLNGKTDTILLDKAF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCEEEHHHHH
NLCAIVEMIQTASLLHDDVIDKATMRRKLPSINALFGNFNAVMLGDVFYSKAFFELSKMG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
ESIAQALSNAVLRLSRGEIEDVFVGECFNSDKQKYWRILEDKTAHFIEASLKSMAILLNK
HHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DAKIYADFGLNFGMAFQIIDDLLDITQDAKTLGKPNFSDFKEGKTTLPYLLLYEKLNQHE
CCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCHHHHHHHHHHHCCCC
QGLLISYFKQDSHEIIEWTKEKFKQYGIIEETLKTAQVYSKKALEAIKKENNLILEKLAQ
CCHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHH
DVIYRTF
HHHHHCC
>Mature Secondary Structure
MQEKQLKAIQNKIASWIKEIESAFIDELFSKIGPSKMLRSKLMLALLNGKTDTILLDKAF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCEEEHHHHH
NLCAIVEMIQTASLLHDDVIDKATMRRKLPSINALFGNFNAVMLGDVFYSKAFFELSKMG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
ESIAQALSNAVLRLSRGEIEDVFVGECFNSDKQKYWRILEDKTAHFIEASLKSMAILLNK
HHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DAKIYADFGLNFGMAFQIIDDLLDITQDAKTLGKPNFSDFKEGKTTLPYLLLYEKLNQHE
CCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCHHHHHHHHHHHCCCC
QGLLISYFKQDSHEIIEWTKEKFKQYGIIEETLKTAQVYSKKALEAIKKENNLILEKLAQ
CCHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHH
DVIYRTF
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8312607; 9278503; 2670911; 8037730 [H]