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The map label for this gene is eno

Identifier: 188526959

GI number: 188526959

Start: 148984

End: 150264

Strand: Direct

Name: eno

Synonym: HPSH_00780

Alternate gene names: 188526959

Gene position: 148984-150264 (Clockwise)

Preceding gene: 188526958

Following gene: 188526960

Centisome position: 9.26

GC content: 43.25

Gene sequence:

>1281_bases
ATGCTAACCATTAAAGATGTTCATGCTTTAGAAGTGATGGATAGTAGGGGCAATCCTACCATTCAAGCCAGCGTGATTTT
AAGCGATAACACTAAAGCGAGCGCGATTGTGCCTAGCGGAGCGAGCACCGGTAAAAGAGAAGCGTTAGAATTAAGGGACA
ATGACAAAACCCGTTTTTTGGGTAAAGGGGTTTTAAGGGCATGCGAAAATGTCAATAGCGTGATCAAACACCATTTAATA
GGGCTTGAAGCGATCAATCAAGCCTTTGTGGATGAGAGGTTAAGGGCTTTAGACGGCACGCCTAATTACGCTAATTTAGG
GGCGAACGCTGTTTTGGGCGTTTCTATGGCGTTAGCAAGGGCTAGCGCGAAGGCTTTAAATCTGCCATTATACCGCTATT
TAGGGGGGGCTAACGCTCTGACTTTGCCTGTGCCGATGCTCAATATCATTAACGGCGGAACGCATGCGAATAATTCCATA
GACTTTCAAGAATACATGATCATGCCTTTAGGGTTTGAGAGTTTTAGAGAAGCCTTAAGAGCGAGTGCGGAAGTTTATCA
CACGCTTAAAAAACTTTTAGATGGAAAGAATCAGCTCACAAGCGTGGGCGATGAGGGGGGTTTTGCGCCTAATTTTAACA
ACAATGTAGAACCCCTTGAAGTCATTTCTCAAGCCATTGAAAAAGCCGGCTATAAATTAGGCGAAGAAATAGCGCTCGCT
TTAGATGTAGCGAGCAGCGAGTTAGTGGATGAAAATTTCAATTACCATTTAAAGGGTGAAAATAAGATTCTAGATTCGCA
TGAATTAGTGGCTTATTATAAAAAGTTTGTGGCAAAATACCCGATTGTGTCTATTGAAGATGGTTTGAGCGAAGACGATT
GGGAGGGTTGGGCGTTTTTAAGCAAGGAATTAGGGCGTCAAATCCAGTTAGTGGGCGATGATTTGTTTGTAACGAACGCA
AGCCTCTTGCAAAAAGGCATTGAAAAAAACATCGCAAACGCCATTTTGATCAAACCCAATCAAATCGGCACCATTAGTGA
GACTTTGGAAACCATAAGATTAGCCAAACACCATGCCTATCAATGCGTGATGAGCCATAGAAGCGGGGAGAGTGAAGACA
GCTTTATCGCTGATTTTGCAGTCGCGCTCAATACGGGAGAAATCAAAACCGGATCCACCGCAAGGAGCGAAAGGATTGCT
AAATACAACCGCCTTTTAGAGATTGAGCATGAATTAAAAGGGGGGATTTATATCGGTAAAGAGTTGTTTAAGCATGGCTA
G

Upstream 100 bases:

>100_bases
ATGAAATCACTCTTAAGATTAAAGAGAGCATTGGCTCTAATGAAGAGATCATGCCCTTACCAGATGAGCCTTTAGAAGAA
ATGGAATAGAAAGGATTTTG

Downstream 100 bases:

>100_bases
TGGCCTTTTTGAAAACGATGAAATCAAAGACAATAAAGCGCGAGATTTTTTCTATAGCCATAGCTCCCTGATTGTCTTTT
TCCTTTTACTGCTTGGGTTT

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase [H]

Number of amino acids: Translated: 426; Mature: 426

Protein sequence:

>426_residues
MLTIKDVHALEVMDSRGNPTIQASVILSDNTKASAIVPSGASTGKREALELRDNDKTRFLGKGVLRACENVNSVIKHHLI
GLEAINQAFVDERLRALDGTPNYANLGANAVLGVSMALARASAKALNLPLYRYLGGANALTLPVPMLNIINGGTHANNSI
DFQEYMIMPLGFESFREALRASAEVYHTLKKLLDGKNQLTSVGDEGGFAPNFNNNVEPLEVISQAIEKAGYKLGEEIALA
LDVASSELVDENFNYHLKGENKILDSHELVAYYKKFVAKYPIVSIEDGLSEDDWEGWAFLSKELGRQIQLVGDDLFVTNA
SLLQKGIEKNIANAILIKPNQIGTISETLETIRLAKHHAYQCVMSHRSGESEDSFIADFAVALNTGEIKTGSTARSERIA
KYNRLLEIEHELKGGIYIGKELFKHG

Sequences:

>Translated_426_residues
MLTIKDVHALEVMDSRGNPTIQASVILSDNTKASAIVPSGASTGKREALELRDNDKTRFLGKGVLRACENVNSVIKHHLI
GLEAINQAFVDERLRALDGTPNYANLGANAVLGVSMALARASAKALNLPLYRYLGGANALTLPVPMLNIINGGTHANNSI
DFQEYMIMPLGFESFREALRASAEVYHTLKKLLDGKNQLTSVGDEGGFAPNFNNNVEPLEVISQAIEKAGYKLGEEIALA
LDVASSELVDENFNYHLKGENKILDSHELVAYYKKFVAKYPIVSIEDGLSEDDWEGWAFLSKELGRQIQLVGDDLFVTNA
SLLQKGIEKNIANAILIKPNQIGTISETLETIRLAKHHAYQCVMSHRSGESEDSFIADFAVALNTGEIKTGSTARSERIA
KYNRLLEIEHELKGGIYIGKELFKHG
>Mature_426_residues
MLTIKDVHALEVMDSRGNPTIQASVILSDNTKASAIVPSGASTGKREALELRDNDKTRFLGKGVLRACENVNSVIKHHLI
GLEAINQAFVDERLRALDGTPNYANLGANAVLGVSMALARASAKALNLPLYRYLGGANALTLPVPMLNIINGGTHANNSI
DFQEYMIMPLGFESFREALRASAEVYHTLKKLLDGKNQLTSVGDEGGFAPNFNNNVEPLEVISQAIEKAGYKLGEEIALA
LDVASSELVDENFNYHLKGENKILDSHELVAYYKKFVAKYPIVSIEDGLSEDDWEGWAFLSKELGRQIQLVGDDLFVTNA
SLLQKGIEKNIANAILIKPNQIGTISETLETIRLAKHHAYQCVMSHRSGESEDSFIADFAVALNTGEIKTGSTARSERIA
KYNRLLEIEHELKGGIYIGKELFKHG

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis [H]

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family [H]

Homologues:

Organism=Homo sapiens, GI5803011, Length=422, Percent_Identity=48.1042654028436, Blast_Score=394, Evalue=1e-109,
Organism=Homo sapiens, GI4503571, Length=422, Percent_Identity=46.4454976303318, Blast_Score=376, Evalue=1e-104,
Organism=Homo sapiens, GI301897477, Length=435, Percent_Identity=45.9770114942529, Blast_Score=374, Evalue=1e-104,
Organism=Homo sapiens, GI301897469, Length=435, Percent_Identity=45.9770114942529, Blast_Score=374, Evalue=1e-104,
Organism=Homo sapiens, GI301897479, Length=433, Percent_Identity=42.4942263279446, Blast_Score=330, Evalue=2e-90,
Organism=Homo sapiens, GI169201331, Length=340, Percent_Identity=26.4705882352941, Blast_Score=113, Evalue=3e-25,
Organism=Homo sapiens, GI169201757, Length=340, Percent_Identity=26.4705882352941, Blast_Score=113, Evalue=3e-25,
Organism=Homo sapiens, GI239744207, Length=340, Percent_Identity=26.4705882352941, Blast_Score=113, Evalue=3e-25,
Organism=Homo sapiens, GI310129182, Length=220, Percent_Identity=26.8181818181818, Blast_Score=66, Evalue=7e-11,
Organism=Homo sapiens, GI310110045, Length=220, Percent_Identity=26.8181818181818, Blast_Score=66, Evalue=7e-11,
Organism=Homo sapiens, GI310120572, Length=220, Percent_Identity=26.8181818181818, Blast_Score=66, Evalue=7e-11,
Organism=Escherichia coli, GI1789141, Length=417, Percent_Identity=52.757793764988, Blast_Score=429, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI71995829, Length=431, Percent_Identity=47.0997679814385, Blast_Score=385, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI17536383, Length=423, Percent_Identity=47.5177304964539, Blast_Score=384, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI32563855, Length=190, Percent_Identity=44.2105263157895, Blast_Score=169, Evalue=2e-42,
Organism=Saccharomyces cerevisiae, GI6321693, Length=439, Percent_Identity=46.0136674259681, Blast_Score=353, Evalue=3e-98,
Organism=Saccharomyces cerevisiae, GI6324974, Length=435, Percent_Identity=43.6781609195402, Blast_Score=338, Evalue=8e-94,
Organism=Saccharomyces cerevisiae, GI6324969, Length=435, Percent_Identity=43.6781609195402, Blast_Score=338, Evalue=8e-94,
Organism=Saccharomyces cerevisiae, GI6323985, Length=435, Percent_Identity=43.6781609195402, Blast_Score=338, Evalue=1e-93,
Organism=Saccharomyces cerevisiae, GI6321968, Length=439, Percent_Identity=46.0136674259681, Blast_Score=335, Evalue=6e-93,
Organism=Drosophila melanogaster, GI24580918, Length=435, Percent_Identity=45.9770114942529, Blast_Score=362, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24580916, Length=435, Percent_Identity=45.9770114942529, Blast_Score=362, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24580920, Length=435, Percent_Identity=45.9770114942529, Blast_Score=362, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24580914, Length=435, Percent_Identity=45.9770114942529, Blast_Score=362, Evalue=1e-100,
Organism=Drosophila melanogaster, GI281360527, Length=435, Percent_Identity=45.9770114942529, Blast_Score=362, Evalue=1e-100,
Organism=Drosophila melanogaster, GI17137654, Length=435, Percent_Identity=45.9770114942529, Blast_Score=362, Evalue=1e-100,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811 [H]

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N [H]

EC number: =4.2.1.11 [H]

Molecular weight: Translated: 46637; Mature: 46637

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: PS00164 ENOLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTIKDVHALEVMDSRGNPTIQASVILSDNTKASAIVPSGASTGKREALELRDNDKTRFL
CCEECCCHHHHHHCCCCCCEEEEEEEEECCCCCEEEECCCCCCCCHHHHHCCCCCCHHHH
GKGVLRACENVNSVIKHHLIGLEAINQAFVDERLRALDGTPNYANLGANAVLGVSMALAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCHHHHHHHHHHHH
ASAKALNLPLYRYLGGANALTLPVPMLNIINGGTHANNSIDFQEYMIMPLGFESFREALR
HHHHHHCCCHHHHHCCCCEEEECCHHHHHHCCCCCCCCCCCHHHHEEECCCHHHHHHHHH
ASAEVYHTLKKLLDGKNQLTSVGDEGGFAPNFNNNVEPLEVISQAIEKAGYKLGEEIALA
HHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHEEE
LDVASSELVDENFNYHLKGENKILDSHELVAYYKKFVAKYPIVSIEDGLSEDDWEGWAFL
EEHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHHCCCEEECCCCCCCCCCHHHHH
SKELGRQIQLVGDDLFVTNASLLQKGIEKNIANAILIKPNQIGTISETLETIRLAKHHAY
HHHHCCEEEEECCCEEEECHHHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHHHHHHH
QCVMSHRSGESEDSFIADFAVALNTGEIKTGSTARSERIAKYNRLLEIEHELKGGIYIGK
HHHHHHCCCCCCCHHHHHHHEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEECH
ELFKHG
HHHHCC
>Mature Secondary Structure
MLTIKDVHALEVMDSRGNPTIQASVILSDNTKASAIVPSGASTGKREALELRDNDKTRFL
CCEECCCHHHHHHCCCCCCEEEEEEEEECCCCCEEEECCCCCCCCHHHHHCCCCCCHHHH
GKGVLRACENVNSVIKHHLIGLEAINQAFVDERLRALDGTPNYANLGANAVLGVSMALAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCHHHHHHHHHHHH
ASAKALNLPLYRYLGGANALTLPVPMLNIINGGTHANNSIDFQEYMIMPLGFESFREALR
HHHHHHCCCHHHHHCCCCEEEECCHHHHHHCCCCCCCCCCCHHHHEEECCCHHHHHHHHH
ASAEVYHTLKKLLDGKNQLTSVGDEGGFAPNFNNNVEPLEVISQAIEKAGYKLGEEIALA
HHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHEEE
LDVASSELVDENFNYHLKGENKILDSHELVAYYKKFVAKYPIVSIEDGLSEDDWEGWAFL
EEHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHHCCCEEECCCCCCCCCCHHHHH
SKELGRQIQLVGDDLFVTNASLLQKGIEKNIANAILIKPNQIGTISETLETIRLAKHHAY
HHHHCCEEEEECCCEEEECHHHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHHHHHHH
QCVMSHRSGESEDSFIADFAVALNTGEIKTGSTARSERIAKYNRLLEIEHELKGGIYIGK
HHHHHHCCCCCCCHHHHHHHEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEECH
ELFKHG
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA