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The map label for this gene is metB [H]

Identifier: 188526911

GI number: 188526911

Start: 100423

End: 101565

Strand: Reverse

Name: metB [H]

Synonym: HPSH_00530

Alternate gene names: 188526911

Gene position: 101565-100423 (Counterclockwise)

Preceding gene: 188526912

Following gene: 188526910

Centisome position: 6.31

GC content: 48.03

Gene sequence:

>1143_bases
ATGCGCATGCAAACAAAATTAATCCATGGGGGCATTAGTGAGGATGCGGCAACGGGGGCGGTGAGTGTGCCTATTTATCA
AACTTCCACCTACCGCCAAGGTGCCATAGGCCGCCACAAGGGCTATGAATACTCTCGCTCAGGCAACCCCACGCGCTTTG
CTTTAGAAGAACTCATCGCTGATTTAGAAGGGGGGGTTAAGGGGTTTGCTTTTGCCTCTGGTTTAGCTGGAATCCATGCC
GTTTTTTCTCTCTTGCAATCAGGCGATCATGTGCTATTAGGCGATGATGTCTATGGGGGGACTTTTCGCTTGTTCAATAA
AGTGCTTGTCAAAAACGGGCTTTCTTGCACCATTATAGACACTAGCGATCTATCCCAAATCAAAAAGGCTATCAAACCCA
ACACCAAAGCCCTTTATTTAGAAACCCCTAGTAACCCCTTGCTTAAAATCACGGATTTAGTGCAATGCGCTAGTGTCGCT
AAAGATCATGGTTTGCTCACTATCGTGGATAACACCTTTGCCACCCCCTATTGCCAAAACCCGCTTCTTTTGGGAGCGGA
TATTGTAGTGCATAGCGGCACCAAATACTTAGGCGGGCATAGCGATGTGGTCGCTGGGCTTGTAACCACTAATAATGAAG
CACTAGCCCAAGAGATCGCTTTTTTCCAAAACGCTATCGGTGGGGTTTTAGGCCCTCAAGACAGCTGGCTGTTGCAAAGA
GGGATTAAAACGCTAGGATTGCGCATGGAGGCCCATCAAAAAAACGCCCTTTGCGTGGCTGAGTTTTTAGAAAAACACCC
TAAAGTGGAGAAGGTCTATTACCCAGGCTTAAGCGCTCACCCTAACCACGAACTGGCCAAAAAACAGATGCGTGGCTTTA
GCGGGATGCTCTCTTTCACTCTCAAAAATGACAGCGAGGCGGTTGCTTTTGTAGAAAGCCTTAAACTATTCATTTTAGGC
GAGAGTTTGGGCGGGGTGGAAAGTTTGGTGGGAGTTCCGGCGCTTATGACCCATGCGTGTATCCCCAAAGCGCAACGAGA
AGCTGCCGGGATTAGAGATGGCTTGGTGCGCTTGTCTGTGGGGATTGAGCATGAACAGGATTTGTTAGAAGATTTAGAGC
AAGCGTTCGCTAAAATAGGCTAA

Upstream 100 bases:

>100_bases
GCCTCCCAGAAGGCAGCCAGGTTTTAACCATTTTCCCGGATGTTGCCGATCGTTATCTCTCAAAAGGTATTTATTTATAA
ATTCATAAAAAGGAAAAATC

Downstream 100 bases:

>100_bases
AGCTTACCATTTATAAAATAAAGGAGTTACAAACATGAAAACGCCAAAAATGAATGTAGAGAGTTTTAATTTGGATCACA
CCAAAGTCAAAGCCCCTTAT

Product: cystathionine gamma-synthase/cystathionine beta-lyase

Products: NA

Alternate protein names: CGS; O-succinylhomoserine (thiol)-lyase [H]

Number of amino acids: Translated: 380; Mature: 380

Protein sequence:

>380_residues
MRMQTKLIHGGISEDAATGAVSVPIYQTSTYRQGAIGRHKGYEYSRSGNPTRFALEELIADLEGGVKGFAFASGLAGIHA
VFSLLQSGDHVLLGDDVYGGTFRLFNKVLVKNGLSCTIIDTSDLSQIKKAIKPNTKALYLETPSNPLLKITDLVQCASVA
KDHGLLTIVDNTFATPYCQNPLLLGADIVVHSGTKYLGGHSDVVAGLVTTNNEALAQEIAFFQNAIGGVLGPQDSWLLQR
GIKTLGLRMEAHQKNALCVAEFLEKHPKVEKVYYPGLSAHPNHELAKKQMRGFSGMLSFTLKNDSEAVAFVESLKLFILG
ESLGGVESLVGVPALMTHACIPKAQREAAGIRDGLVRLSVGIEHEQDLLEDLEQAFAKIG

Sequences:

>Translated_380_residues
MRMQTKLIHGGISEDAATGAVSVPIYQTSTYRQGAIGRHKGYEYSRSGNPTRFALEELIADLEGGVKGFAFASGLAGIHA
VFSLLQSGDHVLLGDDVYGGTFRLFNKVLVKNGLSCTIIDTSDLSQIKKAIKPNTKALYLETPSNPLLKITDLVQCASVA
KDHGLLTIVDNTFATPYCQNPLLLGADIVVHSGTKYLGGHSDVVAGLVTTNNEALAQEIAFFQNAIGGVLGPQDSWLLQR
GIKTLGLRMEAHQKNALCVAEFLEKHPKVEKVYYPGLSAHPNHELAKKQMRGFSGMLSFTLKNDSEAVAFVESLKLFILG
ESLGGVESLVGVPALMTHACIPKAQREAAGIRDGLVRLSVGIEHEQDLLEDLEQAFAKIG
>Mature_380_residues
MRMQTKLIHGGISEDAATGAVSVPIYQTSTYRQGAIGRHKGYEYSRSGNPTRFALEELIADLEGGVKGFAFASGLAGIHA
VFSLLQSGDHVLLGDDVYGGTFRLFNKVLVKNGLSCTIIDTSDLSQIKKAIKPNTKALYLETPSNPLLKITDLVQCASVA
KDHGLLTIVDNTFATPYCQNPLLLGADIVVHSGTKYLGGHSDVVAGLVTTNNEALAQEIAFFQNAIGGVLGPQDSWLLQR
GIKTLGLRMEAHQKNALCVAEFLEKHPKVEKVYYPGLSAHPNHELAKKQMRGFSGMLSFTLKNDSEAVAFVESLKLFILG
ESLGGVESLVGVPALMTHACIPKAQREAAGIRDGLVRLSVGIEHEQDLLEDLEQAFAKIG

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=360, Percent_Identity=50, Blast_Score=357, Evalue=7e-99,
Organism=Homo sapiens, GI299473759, Length=360, Percent_Identity=45.2777777777778, Blast_Score=307, Evalue=9e-84,
Organism=Homo sapiens, GI25453487, Length=359, Percent_Identity=44.8467966573816, Blast_Score=293, Evalue=1e-79,
Organism=Escherichia coli, GI1790375, Length=377, Percent_Identity=41.3793103448276, Blast_Score=304, Evalue=5e-84,
Organism=Escherichia coli, GI1789383, Length=398, Percent_Identity=30.9045226130653, Blast_Score=190, Evalue=1e-49,
Organism=Caenorhabditis elegans, GI17560056, Length=364, Percent_Identity=48.9010989010989, Blast_Score=352, Evalue=2e-97,
Organism=Caenorhabditis elegans, GI17538055, Length=364, Percent_Identity=48.9010989010989, Blast_Score=351, Evalue=5e-97,
Organism=Caenorhabditis elegans, GI71980708, Length=370, Percent_Identity=42.1621621621622, Blast_Score=292, Evalue=2e-79,
Organism=Saccharomyces cerevisiae, GI6319307, Length=387, Percent_Identity=47.0284237726098, Blast_Score=327, Evalue=2e-90,
Organism=Saccharomyces cerevisiae, GI6321254, Length=406, Percent_Identity=33.743842364532, Blast_Score=198, Evalue=1e-51,
Organism=Saccharomyces cerevisiae, GI6321170, Length=330, Percent_Identity=36.6666666666667, Blast_Score=187, Evalue=2e-48,
Organism=Saccharomyces cerevisiae, GI6323333, Length=422, Percent_Identity=33.8862559241706, Blast_Score=184, Evalue=2e-47,
Organism=Saccharomyces cerevisiae, GI6321904, Length=394, Percent_Identity=29.6954314720812, Blast_Score=135, Evalue=8e-33,
Organism=Saccharomyces cerevisiae, GI6322970, Length=365, Percent_Identity=26.5753424657534, Blast_Score=89, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6323557, Length=343, Percent_Identity=27.4052478134111, Blast_Score=87, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6322590, Length=257, Percent_Identity=29.5719844357977, Blast_Score=80, Evalue=7e-16,
Organism=Drosophila melanogaster, GI19922554, Length=378, Percent_Identity=50.5291005291005, Blast_Score=377, Evalue=1e-105,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =2.5.1.48 [H]

Molecular weight: Translated: 40854; Mature: 40854

Theoretical pI: Translated: 6.84; Mature: 6.84

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRMQTKLIHGGISEDAATGAVSVPIYQTSTYRQGAIGRHKGYEYSRSGNPTRFALEELIA
CCCCHHHHCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
DLEGGVKGFAFASGLAGIHAVFSLLQSGDHVLLGDDVYGGTFRLFNKVLVKNGLSCTIID
HHHCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHCCCEEEEEC
TSDLSQIKKAIKPNTKALYLETPSNPLLKITDLVQCASVAKDHGLLTIVDNTFATPYCQN
CCCHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCC
PLLLGADIVVHSGTKYLGGHSDVVAGLVTTNNEALAQEIAFFQNAIGGVLGPQDSWLLQR
CEEEECEEEEECCCCCCCCCHHHEEEEEECCCHHHHHHHHHHHHHHCCCCCCCHHHHHHH
GIKTLGLRMEAHQKNALCVAEFLEKHPKVEKVYYPGLSAHPNHELAKKQMRGFSGMLSFT
HHHHHCCEEEHHCCCHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHCCCCEEEEE
LKNDSEAVAFVESLKLFILGESLGGVESLVGVPALMTHACIPKAQREAAGIRDGLVRLSV
ECCCCHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHHCCHHHHHHCCHHHCCEEEEE
GIEHEQDLLEDLEQAFAKIG
CCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRMQTKLIHGGISEDAATGAVSVPIYQTSTYRQGAIGRHKGYEYSRSGNPTRFALEELIA
CCCCHHHHCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
DLEGGVKGFAFASGLAGIHAVFSLLQSGDHVLLGDDVYGGTFRLFNKVLVKNGLSCTIID
HHHCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHCCCEEEEEC
TSDLSQIKKAIKPNTKALYLETPSNPLLKITDLVQCASVAKDHGLLTIVDNTFATPYCQN
CCCHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCC
PLLLGADIVVHSGTKYLGGHSDVVAGLVTTNNEALAQEIAFFQNAIGGVLGPQDSWLLQR
CEEEECEEEEECCCCCCCCCHHHEEEEEECCCHHHHHHHHHHHHHHCCCCCCCHHHHHHH
GIKTLGLRMEAHQKNALCVAEFLEKHPKVEKVYYPGLSAHPNHELAKKQMRGFSGMLSFT
HHHHHCCEEEHHCCCHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHCCCCEEEEE
LKNDSEAVAFVESLKLFILGESLGGVESLVGVPALMTHACIPKAQREAAGIRDGLVRLSV
ECCCCHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHHCCHHHHHHCCHHHCCEEEEE
GIEHEQDLLEDLEQAFAKIG
CCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]