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The map label for this gene is pyk1 [H]

Identifier: 187935594

GI number: 187935594

Start: 2915512

End: 2916531

Strand: Reverse

Name: pyk1 [H]

Synonym: CLL_A2841

Alternate gene names: 187935594

Gene position: 2916531-2915512 (Counterclockwise)

Preceding gene: 187933058

Following gene: 187932572

Centisome position: 76.74

GC content: 24.61

Gene sequence:

>1020_bases
ATGTATATTATTGCTACAGTAGGACCTAATATAATGGATAAAAAAGTATTAAAAGATATATTTTATAGCGGTGCAAATTC
ATTAAGATTTAACTTTTCACATGGCAATAGTAAGGAATTTTTAGAATATATTGAAATGGCAAAGGCGATAAAAGAAGATG
TAGTTATAATATTAGATTTGTGTGGAAGCAAAATAAGAGTTTCTAATAAGCTTTTTGGTATATGCAGAATATATAATGAA
GAAAAAGTTTATTTTTGTGGAGAAGATAAATATAAACAAGTAAATCAAAAATTAAAAAATAATAATAAAATAGTTCCTTT
AAATATAAGTAATAATGTTCTTATGGAAAATAATTATGATGAAATAAGTATTAAAGATAATACTATGATATTTAATATAT
TAGGAAAAGAAGAGGGTTTTTTAAAAGCAGATACTGTAAGAGGTGGAGTTATACGGGCTGGAAAAGGATGCAATATAAAA
GGTTTTGATAGAAGTAAAATAAAACTTAACTTAAAAGATAAAGAGGATATAAAATTTGGAATAGAACATAAGGTAGATAT
AATATGTCAATCATTTGTTGAAAGTGATGAATGTATAAATGAAATAATAGAATTTATAGATTTAAATAAAAAAGAAGATT
ATAATCCTAAAATATGGGCTAAAATTGAAACTTTAGAGGGAATAAAAAATATAGATAAGATAGTATCTAAAGTAGGGGGG
ATTATGGTTGGAAGAGGTGATTTGATAGCTGAGACTTCCATAGAAGATACACCTATATATGAAGAATTTATAATAAAAAG
AGTAAAAGAATATACTGATAAAGAAATTATAATAGCGACACACATTTTAGATAATATGAAAAGTGGAAAAATGCCATGTA
TAAGTGAAGTGGAAAGTATATATAACTTTATTAAAAATGGAGTAGATGGTTTTTTATTAGCAGGAGAAACTTCTATTGGG
AGAGCTCCTATAAAAACAGTTAAATTTTTAAAAGACCTAATAGAAAAGTATGAAGGATAA

Upstream 100 bases:

>100_bases
TGAAATTTAAAACATATCCCAATAAAGATATTATTGTGATATTTATAATTTTAATGAGTATGAATTATAAAGAGAGATAA
TAGTTTTTTAAGGAGGGTAT

Downstream 100 bases:

>100_bases
AAAAATATATGATGTATTAAAAGATGGCTTAATGTCTATAACATGGGTAATTATAATGATAGTTACTTTTAAATTATTGA
AGATAGTTTATAAAATGTTT

Product: pyruvate kinase, barrel domain

Products: NA

Alternate protein names: PK 1 [H]

Number of amino acids: Translated: 339; Mature: 339

Protein sequence:

>339_residues
MYIIATVGPNIMDKKVLKDIFYSGANSLRFNFSHGNSKEFLEYIEMAKAIKEDVVIILDLCGSKIRVSNKLFGICRIYNE
EKVYFCGEDKYKQVNQKLKNNNKIVPLNISNNVLMENNYDEISIKDNTMIFNILGKEEGFLKADTVRGGVIRAGKGCNIK
GFDRSKIKLNLKDKEDIKFGIEHKVDIICQSFVESDECINEIIEFIDLNKKEDYNPKIWAKIETLEGIKNIDKIVSKVGG
IMVGRGDLIAETSIEDTPIYEEFIIKRVKEYTDKEIIIATHILDNMKSGKMPCISEVESIYNFIKNGVDGFLLAGETSIG
RAPIKTVKFLKDLIEKYEG

Sequences:

>Translated_339_residues
MYIIATVGPNIMDKKVLKDIFYSGANSLRFNFSHGNSKEFLEYIEMAKAIKEDVVIILDLCGSKIRVSNKLFGICRIYNE
EKVYFCGEDKYKQVNQKLKNNNKIVPLNISNNVLMENNYDEISIKDNTMIFNILGKEEGFLKADTVRGGVIRAGKGCNIK
GFDRSKIKLNLKDKEDIKFGIEHKVDIICQSFVESDECINEIIEFIDLNKKEDYNPKIWAKIETLEGIKNIDKIVSKVGG
IMVGRGDLIAETSIEDTPIYEEFIIKRVKEYTDKEIIIATHILDNMKSGKMPCISEVESIYNFIKNGVDGFLLAGETSIG
RAPIKTVKFLKDLIEKYEG
>Mature_339_residues
MYIIATVGPNIMDKKVLKDIFYSGANSLRFNFSHGNSKEFLEYIEMAKAIKEDVVIILDLCGSKIRVSNKLFGICRIYNE
EKVYFCGEDKYKQVNQKLKNNNKIVPLNISNNVLMENNYDEISIKDNTMIFNILGKEEGFLKADTVRGGVIRAGKGCNIK
GFDRSKIKLNLKDKEDIKFGIEHKVDIICQSFVESDECINEIIEFIDLNKKEDYNPKIWAKIETLEGIKNIDKIVSKVGG
IMVGRGDLIAETSIEDTPIYEEFIIKRVKEYTDKEIIIATHILDNMKSGKMPCISEVESIYNFIKNGVDGFLLAGETSIG
RAPIKTVKFLKDLIEKYEG

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI32967597, Length=352, Percent_Identity=29.5454545454545, Blast_Score=127, Evalue=1e-29,
Organism=Homo sapiens, GI10835121, Length=352, Percent_Identity=29.5454545454545, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI33286418, Length=350, Percent_Identity=28.2857142857143, Blast_Score=119, Evalue=3e-27,
Organism=Homo sapiens, GI33286422, Length=350, Percent_Identity=28.2857142857143, Blast_Score=119, Evalue=3e-27,
Organism=Homo sapiens, GI33286420, Length=350, Percent_Identity=28.2857142857143, Blast_Score=119, Evalue=3e-27,
Organism=Homo sapiens, GI310128732, Length=295, Percent_Identity=28.135593220339, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI310128730, Length=295, Percent_Identity=28.135593220339, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI310128736, Length=243, Percent_Identity=26.7489711934156, Blast_Score=69, Evalue=7e-12,
Organism=Homo sapiens, GI310128734, Length=243, Percent_Identity=26.7489711934156, Blast_Score=69, Evalue=7e-12,
Organism=Escherichia coli, GI1787965, Length=353, Percent_Identity=28.328611898017, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1788160, Length=344, Percent_Identity=27.0348837209302, Blast_Score=103, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17544584, Length=350, Percent_Identity=27.1428571428571, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17506831, Length=357, Percent_Identity=26.6106442577031, Blast_Score=101, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI17506829, Length=357, Percent_Identity=26.6106442577031, Blast_Score=100, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI71984406, Length=357, Percent_Identity=26.6106442577031, Blast_Score=100, Evalue=8e-22,
Organism=Caenorhabditis elegans, GI71984413, Length=357, Percent_Identity=26.6106442577031, Blast_Score=100, Evalue=8e-22,
Organism=Saccharomyces cerevisiae, GI6319279, Length=337, Percent_Identity=28.1899109792285, Blast_Score=123, Evalue=3e-29,
Organism=Saccharomyces cerevisiae, GI6324923, Length=346, Percent_Identity=27.4566473988439, Blast_Score=117, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24648964, Length=340, Percent_Identity=28.5294117647059, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI28571814, Length=340, Percent_Identity=28.5294117647059, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24648966, Length=344, Percent_Identity=27.6162790697674, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24646914, Length=168, Percent_Identity=29.7619047619048, Blast_Score=91, Evalue=8e-19,
Organism=Drosophila melanogaster, GI24581235, Length=353, Percent_Identity=24.6458923512748, Blast_Score=85, Evalue=5e-17,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 38561; Mature: 38561

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYIIATVGPNIMDKKVLKDIFYSGANSLRFNFSHGNSKEFLEYIEMAKAIKEDVVIILDL
CEEEEECCCCHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCEEEEEEC
CGSKIRVSNKLFGICRIYNEEKVYFCGEDKYKQVNQKLKNNNKIVPLNISNNVLMENNYD
CCCEEEECCCEEEEEEEECCCEEEEECCHHHHHHHHHHHCCCCEEEEECCCCEEEECCCC
EISIKDNTMIFNILGKEEGFLKADTVRGGVIRAGKGCNIKGFDRSKIKLNLKDKEDIKFG
EEEEECCEEEEEEECCCCCCEEECCCCCCEEECCCCCCCCCCCCCEEEEECCCCCCCEEC
IEHKVDIICQSFVESDECINEIIEFIDLNKKEDYNPKIWAKIETLEGIKNIDKIVSKVGG
CCHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHCC
IMVGRGDLIAETSIEDTPIYEEFIIKRVKEYTDKEIIIATHILDNMKSGKMPCISEVESI
EEECCCCEEEECCCCCCCCHHHHHHHHHHHHCCCEEEEEHHHHHCCCCCCCCHHHHHHHH
YNFIKNGVDGFLLAGETSIGRAPIKTVKFLKDLIEKYEG
HHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MYIIATVGPNIMDKKVLKDIFYSGANSLRFNFSHGNSKEFLEYIEMAKAIKEDVVIILDL
CEEEEECCCCHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCEEEEEEC
CGSKIRVSNKLFGICRIYNEEKVYFCGEDKYKQVNQKLKNNNKIVPLNISNNVLMENNYD
CCCEEEECCCEEEEEEEECCCEEEEECCHHHHHHHHHHHCCCCEEEEECCCCEEEECCCC
EISIKDNTMIFNILGKEEGFLKADTVRGGVIRAGKGCNIKGFDRSKIKLNLKDKEDIKFG
EEEEECCEEEEEEECCCCCCEEECCCCCCEEECCCCCCCCCCCCCEEEEECCCCCCCEEC
IEHKVDIICQSFVESDECINEIIEFIDLNKKEDYNPKIWAKIETLEGIKNIDKIVSKVGG
CCHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHCC
IMVGRGDLIAETSIEDTPIYEEFIIKRVKEYTDKEIIIATHILDNMKSGKMPCISEVESI
EEECCCCEEEECCCCCCCCHHHHHHHHHHHHCCCEEEEEHHHHHCCCCCCCCHHHHHHHH
YNFIKNGVDGFLLAGETSIGRAPIKTVKFLKDLIEKYEG
HHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]