Click here to switch to the map view.

The map label for this gene is hmp [H]

Identifier: 187935136

GI number: 187935136

Start: 2135303

End: 2136073

Strand: Reverse

Name: hmp [H]

Synonym: CLL_A2041

Alternate gene names: 187935136

Gene position: 2136073-2135303 (Counterclockwise)

Preceding gene: 187933934

Following gene: 187932647

Centisome position: 56.21

GC content: 28.53

Gene sequence:

>771_bases
ATGATAGATAATAAGGTGAGTTCAGATAGTAGATGGAAGGGATTTAAAGACTTTCGAGTATTTAAAATAGTTAAAGAGGA
TGAGGTAGTTACATCATTCTATTTAAAATCATTAGATGGAAGTAAACTTCCAGAATTTATAGCAGGACAATTTATAACTG
TCAGAATTAAAAATGAAGATAATACTTTCACTAAGCCTAGACAATATACATTGTCTATGAATTATAATGAAGAATTTTAT
AGAATAAGTGTAAAGAGAGAAGAGAATGGTTTCTTAAGCAAAAAATTATGTGATGAAATAAAGGCAGGGGACAATCTTCA
AATAACTGCACCGCTTGGAAATTTCATATTAAAGAATAGTGAAAAACCATTAGTTTTAATAGGAGGAGGAATTGGAATAA
CACCAATGCTTACAATGGCTTATGATGCAGTAAGTAGTGATAGAAAAATACATTTTATTTATAGCATACCTAATTCAACA
CATCATAGTTTTAAGGAAGAAACAGCAAAATTAAATAATAATAACTTCAAAAGTACTGTATTATATACACGTCCTACCGA
AACTGAGGAATTAGGAAAAGATTTTGATATAAAGGGTAGAATATCAAGGGAATGGATGATAGATAACTTACCAAAAGATG
GAGAATTTTATTTTTGTGGGCCAGTACCATTTATGAAAACTATCTATCATAATTTAATATCAATGGGAATTGAAAAGGAA
TATATAAATTTTGAAATGTTTGAAGCAGGAGTAGATATAACAAAAGAGTAA

Upstream 100 bases:

>100_bases
ATTTATTTTTGTGAAGATATAATAAGCACATAAGTTCAATCAAACAAGTTTAAGAAAAATAAGCTTGAATTTGATAACTA
AAGAAAAATGGAGGATTTCA

Downstream 100 bases:

>100_bases
AAAAATTATAAACAAATAAGTACAAGAGAGATCGCTAGGAGGAAATAAATTATGTTTAATCATATAAAAGATGAAAACAA
TACTTTTAAAAATTTAATAA

Product: oxidoreductase NAD-binding domain protein

Products: NA

Alternate protein names: Flavohemoglobin; Hemoglobin-like protein; Nitric oxide dioxygenase; NO oxygenase; NOD [H]

Number of amino acids: Translated: 256; Mature: 256

Protein sequence:

>256_residues
MIDNKVSSDSRWKGFKDFRVFKIVKEDEVVTSFYLKSLDGSKLPEFIAGQFITVRIKNEDNTFTKPRQYTLSMNYNEEFY
RISVKREENGFLSKKLCDEIKAGDNLQITAPLGNFILKNSEKPLVLIGGGIGITPMLTMAYDAVSSDRKIHFIYSIPNST
HHSFKEETAKLNNNNFKSTVLYTRPTETEELGKDFDIKGRISREWMIDNLPKDGEFYFCGPVPFMKTIYHNLISMGIEKE
YINFEMFEAGVDITKE

Sequences:

>Translated_256_residues
MIDNKVSSDSRWKGFKDFRVFKIVKEDEVVTSFYLKSLDGSKLPEFIAGQFITVRIKNEDNTFTKPRQYTLSMNYNEEFY
RISVKREENGFLSKKLCDEIKAGDNLQITAPLGNFILKNSEKPLVLIGGGIGITPMLTMAYDAVSSDRKIHFIYSIPNST
HHSFKEETAKLNNNNFKSTVLYTRPTETEELGKDFDIKGRISREWMIDNLPKDGEFYFCGPVPFMKTIYHNLISMGIEKE
YINFEMFEAGVDITKE
>Mature_256_residues
MIDNKVSSDSRWKGFKDFRVFKIVKEDEVVTSFYLKSLDGSKLPEFIAGQFITVRIKNEDNTFTKPRQYTLSMNYNEEFY
RISVKREENGFLSKKLCDEIKAGDNLQITAPLGNFILKNSEKPLVLIGGGIGITPMLTMAYDAVSSDRKIHFIYSIPNST
HHSFKEETAKLNNNNFKSTVLYTRPTETEELGKDFDIKGRISREWMIDNLPKDGEFYFCGPVPFMKTIYHNLISMGIEKE
YINFEMFEAGVDITKE

Specific function: Is involved in NO detoxification in an aerobic process, termed nitric oxide dioxygenase (NOD) reaction that utilizes O(2) and NAD(P)H to convert NO to nitrate, which protects the bacterium from various noxious nitrogen compounds. Therefore, plays a centra

COG id: COG1018

COG function: function code C; Flavodoxin reductases (ferredoxin-NADPH reductases) family 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Escherichia coli, GI1788903, Length=254, Percent_Identity=30.3149606299213, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI1788104, Length=232, Percent_Identity=25.8620689655172, Blast_Score=82, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6321673, Length=268, Percent_Identity=26.4925373134328, Blast_Score=86, Evalue=8e-18,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017927
- InterPro:   IPR001709
- InterPro:   IPR012292
- InterPro:   IPR009050
- InterPro:   IPR000971
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR001221
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00042 Globin; PF00175 NAD_binding_1 [H]

EC number: =1.14.12.17 [H]

Molecular weight: Translated: 29563; Mature: 29563

Theoretical pI: Translated: 6.54; Mature: 6.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDNKVSSDSRWKGFKDFRVFKIVKEDEVVTSFYLKSLDGSKLPEFIAGQFITVRIKNED
CCCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHCCCCCCCHHHCCCEEEEEEECCC
NTFTKPRQYTLSMNYNEEFYRISVKREENGFLSKKLCDEIKAGDNLQITAPLGNFILKNS
CCCCCCEEEEEEECCCCEEEEEEEEECCCCCHHHHHHHHHCCCCCEEEEECCCCEEEECC
EKPLVLIGGGIGITPMLTMAYDAVSSDRKIHFIYSIPNSTHHSFKEETAKLNNNNFKSTV
CCCEEEEECCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCCEEEE
LYTRPTETEELGKDFDIKGRISREWMIDNLPKDGEFYFCGPVPFMKTIYHNLISMGIEKE
EEECCCCHHHHCCCCCCCCCCCCEEHCCCCCCCCCEEEECCCHHHHHHHHHHHHHCCCHH
YINFEMFEAGVDITKE
HCCEEEEECCCCCCCC
>Mature Secondary Structure
MIDNKVSSDSRWKGFKDFRVFKIVKEDEVVTSFYLKSLDGSKLPEFIAGQFITVRIKNED
CCCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHCCCCCCCHHHCCCEEEEEEECCC
NTFTKPRQYTLSMNYNEEFYRISVKREENGFLSKKLCDEIKAGDNLQITAPLGNFILKNS
CCCCCCEEEEEEECCCCEEEEEEEEECCCCCHHHHHHHHHCCCCCEEEEECCCCEEEECC
EKPLVLIGGGIGITPMLTMAYDAVSSDRKIHFIYSIPNSTHHSFKEETAKLNNNNFKSTV
CCCEEEEECCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCCEEEE
LYTRPTETEELGKDFDIKGRISREWMIDNLPKDGEFYFCGPVPFMKTIYHNLISMGIEKE
EEECCCCHHHHCCCCCCCCCCCCEEHCCCCCCCCCEEEECCCHHHHHHHHHHHHHCCCHH
YINFEMFEAGVDITKE
HCCEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]