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The map label for this gene is sleB [H]

Identifier: 187935089

GI number: 187935089

Start: 289714

End: 290343

Strand: Reverse

Name: sleB [H]

Synonym: CLL_A0291

Alternate gene names: 187935089

Gene position: 290343-289714 (Counterclockwise)

Preceding gene: 187933825

Following gene: 187934159

Centisome position: 7.64

GC content: 28.41

Gene sequence:

>630_bases
ATGAAAAAAATATATAGCCTTTTCATAATTAGTTTAGTGCTATATCTTTCCGTTATTAATAACAATGTCGCTTATGCACA
AGTATTAAGTGATAACTTTCATACTAATGTCATTTCTAAAGAGGAAAATAATGAAACTTATTTAAATAATATAAACCTTG
CAACTCAATGCAATGCAGAACTAAATGAAAATACTACAAATAAAAATATTGACGATTCTACAATAGAAGTTTTTAGTTAT
GATACTCAAAAACTCTATATAACGGAAAATGATATTGAATTAATGGCTAAATTAGTTTATGCAGAAAGTATTGGTGAACC
TTATGACGGAAAAGTAGCTGTAGCGTCAGTTGTCTTAAATCGTGTTATAAATCCCAGCTTTCCGAATTCAATAAAAGAAG
TAATTTTTCAGAAAAATGCATTTTCATGTGTTAAAAATGGAGATATTAAAGCTACTCCAAATCAAACATGTTACAATGCA
GTTTATGATGCTATAAGAGGATATGACCCTACAAATGAAGCTTTATTCTTTTATAATCCTAGTACGGCAACTTGTGCATG
GATGCACCAAACACAAAAACAAGATATGAAAACAATAGGGCATCATACGTTTTTCAAAATTAAATCTTAA

Upstream 100 bases:

>100_bases
ACAAAAGGCCATATTATCATATAAACATTAATTTATGTATATAAGACTCTTTTTGGGCAATAATATTATTGGTTTGAAAT
ATACCAGAGGAGGATTTTTA

Downstream 100 bases:

>100_bases
TAAAAATAATGACCAATCTAATAAAATTAGATTGGTCATTATTTTTAGATTTACTTTTATCTATACCTTAAATAGTATTT
CTAAAAATCATAATAACATT

Product: spore-cortex-lytic enzyme, sleb

Products: NA

Alternate protein names: SCLE; Germination-specific amidase [H]

Number of amino acids: Translated: 209; Mature: 209

Protein sequence:

>209_residues
MKKIYSLFIISLVLYLSVINNNVAYAQVLSDNFHTNVISKEENNETYLNNINLATQCNAELNENTTNKNIDDSTIEVFSY
DTQKLYITENDIELMAKLVYAESIGEPYDGKVAVASVVLNRVINPSFPNSIKEVIFQKNAFSCVKNGDIKATPNQTCYNA
VYDAIRGYDPTNEALFFYNPSTATCAWMHQTQKQDMKTIGHHTFFKIKS

Sequences:

>Translated_209_residues
MKKIYSLFIISLVLYLSVINNNVAYAQVLSDNFHTNVISKEENNETYLNNINLATQCNAELNENTTNKNIDDSTIEVFSY
DTQKLYITENDIELMAKLVYAESIGEPYDGKVAVASVVLNRVINPSFPNSIKEVIFQKNAFSCVKNGDIKATPNQTCYNA
VYDAIRGYDPTNEALFFYNPSTATCAWMHQTQKQDMKTIGHHTFFKIKS
>Mature_209_residues
MKKIYSLFIISLVLYLSVINNNVAYAQVLSDNFHTNVISKEENNETYLNNINLATQCNAELNENTTNKNIDDSTIEVFSY
DTQKLYITENDIELMAKLVYAESIGEPYDGKVAVASVVLNRVINPSFPNSIKEVIFQKNAFSCVKNGDIKATPNQTCYNA
VYDAIRGYDPTNEALFFYNPSTATCAWMHQTQKQDMKTIGHHTFFKIKS

Specific function: Probable N-acetylmuramyl-L-alanine amidase. Required for spore cortex hydrolysis during germination. May form a complex with some hydrophobic spore component, leading to a stabilization of the enzyme in a spore-bound form [H]

COG id: COG3773

COG function: function code M; Cell wall hydrolyses involved in spore germination

Gene ontology:

Cell location: Forespore. Note=Expressed in the forespore and then transported across the inner forespore membrane and deposited on the outside of the cortex [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sleB family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011105
- InterPro:   IPR002477
- InterPro:   IPR014224 [H]

Pfam domain/function: PF07486 Hydrolase_2; PF01471 PG_binding_1 [H]

EC number: NA

Molecular weight: Translated: 23699; Mature: 23699

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIYSLFIISLVLYLSVINNNVAYAQVLSDNFHTNVISKEENNETYLNNINLATQCNAE
CHHHHHHHHHHHHHHHHHHCCCCEEEEEHHCCCCCCEECCCCCCCEEEECCCEEEECCCC
LNENTTNKNIDDSTIEVFSYDTQKLYITENDIELMAKLVYAESIGEPYDGKVAVASVVLN
CCCCCCCCCCCCCEEEEEEECCEEEEEECCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
RVINPSFPNSIKEVIFQKNAFSCVKNGDIKATPNQTCYNAVYDAIRGYDPTNEALFFYNP
HHCCCCCCHHHHHHHHCCCHHHHHCCCCEEECCCCHHHHHHHHHHHCCCCCCCEEEEECC
STATCAWMHQTQKQDMKTIGHHTFFKIKS
CCCEEHHHHHHHHHHHHHHCCCEEEEECC
>Mature Secondary Structure
MKKIYSLFIISLVLYLSVINNNVAYAQVLSDNFHTNVISKEENNETYLNNINLATQCNAE
CHHHHHHHHHHHHHHHHHHCCCCEEEEEHHCCCCCCEECCCCCCCEEEECCCEEEECCCC
LNENTTNKNIDDSTIEVFSYDTQKLYITENDIELMAKLVYAESIGEPYDGKVAVASVVLN
CCCCCCCCCCCCCEEEEEEECCEEEEEECCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
RVINPSFPNSIKEVIFQKNAFSCVKNGDIKATPNQTCYNAVYDAIRGYDPTNEALFFYNP
HHCCCCCCHHHHHHHHCCCHHHHHCCCCEEECCCCHHHHHHHHHHHCCCCCCCEEEEECC
STATCAWMHQTQKQDMKTIGHHTFFKIKS
CCCEEHHHHHHHHHHHHHHCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8752358; 8081503; 10197998 [H]