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The map label for this gene is cobS [H]

Identifier: 187934367

GI number: 187934367

Start: 3004532

End: 3005290

Strand: Reverse

Name: cobS [H]

Synonym: CLL_A2922

Alternate gene names: 187934367

Gene position: 3005290-3004532 (Counterclockwise)

Preceding gene: 187935381

Following gene: 187933846

Centisome position: 79.08

GC content: 25.16

Gene sequence:

>759_bases
ATGAAAAATTTATTTAAAGGGCTTATGATGTCTTTAAGTATGTTTACTATAATTCCAATGCCATATGTAGAATGGGATGA
AGATGGAGCTAAAAACATGATGAAGTGTTATCCAATTATAGGCCTTATTGTAGGATGCGTATGGTTTCTAGGATATAAAT
TAATAAATTATTTAAATATATCTATAGTTTTAAAAAGTGCATTAATAATGATAATACCATTCATAATTACTGGAATGTTA
CATTTAGATGGATTTATGGATGTATGTGATGCTATTTTATCAAGAAGAGATAAAGAAGAAAAATTAAGAATATTAAAAGA
TTCTACAACAGGAGCATTTTCAGTTATATCTGTTATTATTTTATTTTTTATACAATTTGGTGCAGTTCATTCATTTTTAG
AATATAATAAAAATCCATATATATTGATGTTTTTACCTATAATAAGTAGAAATATAGTAGCATATTTTTTTATTACTATA
ATTACAATTAAAGAAAGTACATTAGGAAGTTATTTTACAAAAGGGACAAACATTAAAGACAAGGTTATTTTAATATTAGA
GTTAGCCTTAGTATGTATATTGTTTGGAATTATACTTGGATATATTGGAATAGTAATTTTACTAATTGTTACAGTGGCTA
TAAGCTTGTGTGTGAAAAAATGCATTAATGAATTTGGTGGAATATCTGGTGATGTAGCAGGATTTTCTCTTGTTGTTGGA
GAATTAGTAGGATTGTTTTCAGCATGTTTATTTACTTAA

Upstream 100 bases:

>100_bases
ATATATGTGATACAGTTATGGAATGTAGCTTTAGTAATGTAAAACTACATAAAGGAAATGAATTATTGACTGTATTAGGG
AAGAAAGGATAAAGAAAGAT

Downstream 100 bases:

>100_bases
AACTTAAAAAACTAATTTTATATATAAGATTAAAGGAAACTAATTAAGAACTTTAAGTAAAAAAGTATAGAATTTAGAAG
AATAGTATGAATTAAAATTT

Product: cobalamin-5-phosphate synthase

Products: GMP; adenosylcobalamin

Alternate protein names: NA

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MKNLFKGLMMSLSMFTIIPMPYVEWDEDGAKNMMKCYPIIGLIVGCVWFLGYKLINYLNISIVLKSALIMIIPFIITGML
HLDGFMDVCDAILSRRDKEEKLRILKDSTTGAFSVISVIILFFIQFGAVHSFLEYNKNPYILMFLPIISRNIVAYFFITI
ITIKESTLGSYFTKGTNIKDKVILILELALVCILFGIILGYIGIVILLIVTVAISLCVKKCINEFGGISGDVAGFSLVVG
ELVGLFSACLFT

Sequences:

>Translated_252_residues
MKNLFKGLMMSLSMFTIIPMPYVEWDEDGAKNMMKCYPIIGLIVGCVWFLGYKLINYLNISIVLKSALIMIIPFIITGML
HLDGFMDVCDAILSRRDKEEKLRILKDSTTGAFSVISVIILFFIQFGAVHSFLEYNKNPYILMFLPIISRNIVAYFFITI
ITIKESTLGSYFTKGTNIKDKVILILELALVCILFGIILGYIGIVILLIVTVAISLCVKKCINEFGGISGDVAGFSLVVG
ELVGLFSACLFT
>Mature_252_residues
MKNLFKGLMMSLSMFTIIPMPYVEWDEDGAKNMMKCYPIIGLIVGCVWFLGYKLINYLNISIVLKSALIMIIPFIITGML
HLDGFMDVCDAILSRRDKEEKLRILKDSTTGAFSVISVIILFFIQFGAVHSFLEYNKNPYILMFLPIISRNIVAYFFITI
ITIKESTLGSYFTKGTNIKDKVILILELALVCILFGIILGYIGIVILLIVTVAISLCVKKCINEFGGISGDVAGFSLVVG
ELVGLFSACLFT

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin) [H]

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family [H]

Homologues:

Organism=Escherichia coli, GI1788301, Length=241, Percent_Identity=24.896265560166, Blast_Score=67, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003805 [H]

Pfam domain/function: PF02654 CobS [H]

EC number: 2.7.8.26

Molecular weight: Translated: 28095; Mature: 28095

Theoretical pI: Translated: 8.02; Mature: 8.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
7.1 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
7.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHCC
>Mature Secondary Structure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HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: adenosylcobinamide-GDP; alpha-ribazole

Specific reaction: adenosylcobinamide-GDP + alpha-ribazole = GMP + adenosylcobalamin

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA