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The map label for this gene is pduD [H]

Identifier: 187933731

GI number: 187933731

Start: 2199164

End: 2199739

Strand: Reverse

Name: pduD [H]

Synonym: CLL_A2104

Alternate gene names: 187933731

Gene position: 2199739-2199164 (Counterclockwise)

Preceding gene: 187932789

Following gene: 187933430

Centisome position: 57.88

GC content: 32.64

Gene sequence:

>576_bases
ATGGAAAATACAACAAATCAATATGTTATGCCAAAGCTTCAGCTTACTGAAGTAGGAGAAGCAAAAGTTGGAACTAAGGT
AGATGAAGTTGTTATAGGATTAGCACCAGCTTTCCTTAAGTTTCAAAGTAAAACATTAGTTAATGTCTCACACAGTGACA
TATTAACAGAAATCATAGCTGGAATAGAAGAAGAAGGACTAAAGGCTCGTGTTGTTAGAGTAATAAGAACATCTGATGTT
TCATTTATATCAAATGATGCAGCAAAACTAAGTGGGTCAGGAATAGGTATAGGAATACAATCAAAAGGAACAACAGTTAT
CCATCAAAAAGATTTACTTCCTTTAAATAACTTAGAATTATTCCCTCAAGCACCACTATTAACATTAGATACTTATAGAT
TAATAGGAAAGAATGCAGCAAAATATGCTAAGGGAGAATCACCAACACCAGTTCCTACAAAGAACGATCAAATGGTTAGA
CCTAAGTTTATGGCAATAGCTGCCCTATTTCACATAAAAGAAACTAAACATGTAGAAATTGGAGCAAAACCAATAGAAAT
TGAAGTTAAATTTTAA

Upstream 100 bases:

>100_bases
AACTGGTTATAGAATTAGTCCAGAGAGATGGGAAGAAATAAAGAACATTCCTAACGTAATAAAACCAGAAGATATAGATT
AAGGGTAGAGGTGAGCAACA

Downstream 100 bases:

>100_bases
TTTGAAGGGAGAACAACTTTTATGGAACAAAGAAAAATGAATCCACAAGATTATCCTTTGGCTACAAAAAGACCTGAAAG
CATAAAGACACCAACAGGAA

Product: propanediol utilization: dehydratase, medium subunit

Products: NA

Alternate protein names: Propanediol utilization protein pduD [H]

Number of amino acids: Translated: 191; Mature: 191

Protein sequence:

>191_residues
MENTTNQYVMPKLQLTEVGEAKVGTKVDEVVIGLAPAFLKFQSKTLVNVSHSDILTEIIAGIEEEGLKARVVRVIRTSDV
SFISNDAAKLSGSGIGIGIQSKGTTVIHQKDLLPLNNLELFPQAPLLTLDTYRLIGKNAAKYAKGESPTPVPTKNDQMVR
PKFMAIAALFHIKETKHVEIGAKPIEIEVKF

Sequences:

>Translated_191_residues
MENTTNQYVMPKLQLTEVGEAKVGTKVDEVVIGLAPAFLKFQSKTLVNVSHSDILTEIIAGIEEEGLKARVVRVIRTSDV
SFISNDAAKLSGSGIGIGIQSKGTTVIHQKDLLPLNNLELFPQAPLLTLDTYRLIGKNAAKYAKGESPTPVPTKNDQMVR
PKFMAIAALFHIKETKHVEIGAKPIEIEVKF
>Mature_191_residues
MENTTNQYVMPKLQLTEVGEAKVGTKVDEVVIGLAPAFLKFQSKTLVNVSHSDILTEIIAGIEEEGLKARVVRVIRTSDV
SFISNDAAKLSGSGIGIGIQSKGTTVIHQKDLLPLNNLELFPQAPLLTLDTYRLIGKNAAKYAKGESPTPVPTKNDQMVR
PKFMAIAALFHIKETKHVEIGAKPIEIEVKF

Specific function: Part of the pduCDE complex that catalyzes the dehydration of 1,2-propanediol to propionaldehyde. Is required for S.typhimurium growth on 1,2-propanediol as the sole carbon and energy source [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diol/glycerol dehydratase medium subunit family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010254
- InterPro:   IPR003208 [H]

Pfam domain/function: PF02288 Dehydratase_MU [H]

EC number: =4.2.1.28 [H]

Molecular weight: Translated: 20834; Mature: 20834

Theoretical pI: Translated: 9.38; Mature: 9.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENTTNQYVMPKLQLTEVGEAKVGTKVDEVVIGLAPAFLKFQSKTLVNVSHSDILTEIIA
CCCCCCCEEECEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHH
GIEEEGLKARVVRVIRTSDVSFISNDAAKLSGSGIGIGIQSKGTTVIHQKDLLPLNNLEL
CCCCCCHHEEEEEEHHCCCCCEECCCCHHCCCCCEEEEECCCCCEEEEECCCCCCCCCEE
FPQAPLLTLDTYRLIGKNAAKYAKGESPTPVPTKNDQMVRPKFMAIAALFHIKETKHVEI
CCCCCEEEEHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEE
GAKPIEIEVKF
CCCEEEEEEEC
>Mature Secondary Structure
MENTTNQYVMPKLQLTEVGEAKVGTKVDEVVIGLAPAFLKFQSKTLVNVSHSDILTEIIA
CCCCCCCEEECEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHH
GIEEEGLKARVVRVIRTSDVSFISNDAAKLSGSGIGIGIQSKGTTVIHQKDLLPLNNLEL
CCCCCCHHEEEEEEHHCCCCCEECCCCHHCCCCCEEEEECCCCCEEEEECCCCCCCCCEE
FPQAPLLTLDTYRLIGKNAAKYAKGESPTPVPTKNDQMVRPKFMAIAALFHIKETKHVEI
CCCCCEEEEHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEE
GAKPIEIEVKF
CCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9352910; 10498708; 11677609 [H]