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The map label for this gene is potD [H]

Identifier: 187933694

GI number: 187933694

Start: 1003041

End: 1004105

Strand: Reverse

Name: potD [H]

Synonym: CLL_A0993

Alternate gene names: 187933694

Gene position: 1004105-1003041 (Counterclockwise)

Preceding gene: 187932927

Following gene: 187935666

Centisome position: 26.42

GC content: 28.92

Gene sequence:

>1065_bases
TTGAAAAAATTTAAATTATTCGGTGCTTTAGCATTAACAAGTTTATTGACTTTAGGTATTTTCTCAGGATGTGCTGACAA
AAACATAGGACAAAATGGTACAGTTTATTTTTATAATTGTGGCGATTATATTGATGAGGATTTATTGTCAAAATTTCAGA
ATGAAACTGGTATTAAAGTTGAATATTCTACTTATGATACAAACGAAATAATGTATCAAAAATTAAAAAGTAGTCCTGGC
AGTTATGATTTAGTTATACCGTCTGATTATATGATTGAAAAAATGACAAAAGAGGATATGATTGACGAATTAGATTTTAA
TAACATACCAAATTATAAGTATATAGACGATAGTTATAAAAATTTATCTTATGATTCAAATAACAAGTATTCTGTTCCTT
ATATGTGGGGTACTATAGGAATAATATACAACCCTGATATAGTCACAGAACCAGTTACAAGTTGGGATATATTATGGAAC
GAAAAATACAATAATAAGCAACTAATAATGTTTGATTCAATGCGTGACACTCTAGCTATTGGATTGAAAAAACTTGGTTA
TTCTATAAATAGTACTAATACTGATGAAATAAATGCAGCTACTAACGAGTTAGTAAAACAAAAACAAAACATTTCGCCTC
TTTATTATGTAGACGAAGTTAAAGATAAAATGATAAGCGAAGAAGCTGTTATGGCTACTGTTTGGTCTGGAGATGCTGCT
TATATCAGATCAGAAAATCCTAAATTGGAGTACGTTATTCCTGAAGAAGGATCTAATAAATGGTTTGATGGTATGGTTGT
TCCAAAGGATGCTCCAAATAAAGAAAATGCGGAAAAATTAATCAACTTCCTTTGTGACCCTGAAAATGCAAAACAAAATG
TTGATTATATTGGATATTCAACACCTAACAAAGGTGCTTATGATATGTTAGAAGATCTCGTTAAAGATGACAAAGGTATT
TATCCATCAAAAGAAGTTTTAGATAAATGTGAAATTTTTACCGATTTAGGTGATTCATTAAAATTATATGATGATGCTTG
GATGAAAATCAAAACAGCTAAATAA

Upstream 100 bases:

>100_bases
AAACCTGAAATTAATGCTCTTTCAACATTAATGTTCTTAACTGTTTTAACTTTATTAATAATAGTAAATAAAAAACAAAA
TCTTACAAGAGGTGAACAAT

Downstream 100 bases:

>100_bases
TAAAAGGATCCTTAGATAAAGTAAACTTTATCTAAGGATCCTTTTTTATAAGATACATTCAAATAAATAACTAATCAGTA
TTCTAGTTTATTTTGTGAAT

Product: spermidine/putrescine ABC transporter spermidine/putrescine-binding protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: SPBP [H]

Number of amino acids: Translated: 354; Mature: 354

Protein sequence:

>354_residues
MKKFKLFGALALTSLLTLGIFSGCADKNIGQNGTVYFYNCGDYIDEDLLSKFQNETGIKVEYSTYDTNEIMYQKLKSSPG
SYDLVIPSDYMIEKMTKEDMIDELDFNNIPNYKYIDDSYKNLSYDSNNKYSVPYMWGTIGIIYNPDIVTEPVTSWDILWN
EKYNNKQLIMFDSMRDTLAIGLKKLGYSINSTNTDEINAATNELVKQKQNISPLYYVDEVKDKMISEEAVMATVWSGDAA
YIRSENPKLEYVIPEEGSNKWFDGMVVPKDAPNKENAEKLINFLCDPENAKQNVDYIGYSTPNKGAYDMLEDLVKDDKGI
YPSKEVLDKCEIFTDLGDSLKLYDDAWMKIKTAK

Sequences:

>Translated_354_residues
MKKFKLFGALALTSLLTLGIFSGCADKNIGQNGTVYFYNCGDYIDEDLLSKFQNETGIKVEYSTYDTNEIMYQKLKSSPG
SYDLVIPSDYMIEKMTKEDMIDELDFNNIPNYKYIDDSYKNLSYDSNNKYSVPYMWGTIGIIYNPDIVTEPVTSWDILWN
EKYNNKQLIMFDSMRDTLAIGLKKLGYSINSTNTDEINAATNELVKQKQNISPLYYVDEVKDKMISEEAVMATVWSGDAA
YIRSENPKLEYVIPEEGSNKWFDGMVVPKDAPNKENAEKLINFLCDPENAKQNVDYIGYSTPNKGAYDMLEDLVKDDKGI
YPSKEVLDKCEIFTDLGDSLKLYDDAWMKIKTAK
>Mature_354_residues
MKKFKLFGALALTSLLTLGIFSGCADKNIGQNGTVYFYNCGDYIDEDLLSKFQNETGIKVEYSTYDTNEIMYQKLKSSPG
SYDLVIPSDYMIEKMTKEDMIDELDFNNIPNYKYIDDSYKNLSYDSNNKYSVPYMWGTIGIIYNPDIVTEPVTSWDILWN
EKYNNKQLIMFDSMRDTLAIGLKKLGYSINSTNTDEINAATNELVKQKQNISPLYYVDEVKDKMISEEAVMATVWSGDAA
YIRSENPKLEYVIPEEGSNKWFDGMVVPKDAPNKENAEKLINFLCDPENAKQNVDYIGYSTPNKGAYDMLEDLVKDDKGI
YPSKEVLDKCEIFTDLGDSLKLYDDAWMKIKTAK

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787367, Length=325, Percent_Identity=40.9230769230769, Blast_Score=241, Evalue=6e-65,
Organism=Escherichia coli, GI1787078, Length=374, Percent_Identity=31.5508021390374, Blast_Score=172, Evalue=3e-44,
Organism=Escherichia coli, GI1787711, Length=259, Percent_Identity=24.3243243243243, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 40511; Mature: 40511

Theoretical pI: Translated: 4.27; Mature: 4.27

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKFKLFGALALTSLLTLGIFSGCADKNIGQNGTVYFYNCGDYIDEDLLSKFQNETGIKV
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCHHHHHHHCCCCCCEE
EYSTYDTNEIMYQKLKSSPGSYDLVIPSDYMIEKMTKEDMIDELDFNNIPNYKYIDDSYK
EEECCCCHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCC
NLSYDSNNKYSVPYMWGTIGIIYNPDIVTEPVTSWDILWNEKYNNKQLIMFDSMRDTLAI
CCCCCCCCCEECCEEEEEEEEEECCCCCCCCCCCEEEEECCCCCCCEEEEECCHHHHHHH
GLKKLGYSINSTNTDEINAATNELVKQKQNISPLYYVDEVKDKMISEEAVMATVWSGDAA
HHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHCCHHEEEEEECCCEE
YIRSENPKLEYVIPEEGSNKWFDGMVVPKDAPNKENAEKLINFLCDPENAKQNVDYIGYS
EEECCCCEEEEECCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCEEECC
TPNKGAYDMLEDLVKDDKGIYPSKEVLDKCEIFTDLGDSLKLYDDAWMKIKTAK
CCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCEEEEEECC
>Mature Secondary Structure
MKKFKLFGALALTSLLTLGIFSGCADKNIGQNGTVYFYNCGDYIDEDLLSKFQNETGIKV
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCHHHHHHHCCCCCCEE
EYSTYDTNEIMYQKLKSSPGSYDLVIPSDYMIEKMTKEDMIDELDFNNIPNYKYIDDSYK
EEECCCCHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCC
NLSYDSNNKYSVPYMWGTIGIIYNPDIVTEPVTSWDILWNEKYNNKQLIMFDSMRDTLAI
CCCCCCCCCEECCEEEEEEEEEECCCCCCCCCCCEEEEECCCCCCCEEEEECCHHHHHHH
GLKKLGYSINSTNTDEINAATNELVKQKQNISPLYYVDEVKDKMISEEAVMATVWSGDAA
HHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHCCHHEEEEEECCCEE
YIRSENPKLEYVIPEEGSNKWFDGMVVPKDAPNKENAEKLINFLCDPENAKQNVDYIGYS
EEECCCCEEEEECCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCEEECC
TPNKGAYDMLEDLVKDDKGIYPSKEVLDKCEIFTDLGDSLKLYDDAWMKIKTAK
CCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1939142; 8905232; 9278503; 9298646; 8194593; 8621624; 8897598 [H]