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The map label for this gene is ychF [H]

Identifier: 187933242

GI number: 187933242

Start: 2535517

End: 2536614

Strand: Reverse

Name: ychF [H]

Synonym: CLL_A2452

Alternate gene names: 187933242

Gene position: 2536614-2535517 (Counterclockwise)

Preceding gene: 187935273

Following gene: 187932533

Centisome position: 66.75

GC content: 31.24

Gene sequence:

>1098_bases
ATGAAGTTAGGAATTGTAGGATTGCCGAATGTAGGTAAAAGTACATTATTTAATGCGATAACAAAAGCTGGAGCAGAATC
AGCTAATTACCCATTCTGTACGATTGAACCAAATGTGGGAGTAGTTAGTGTTCCGGATAAGAGATTAGATGTCTTAGAAA
AAATGTATAACACAAAGAAAAAAATATATACAGCAATAGAATTCTATGATATAGCAGGATTAGTTAAAGGAGCTTCAAAA
GGTGAAGGTTTAGGAAATAAATTTTTAGCTAACATTAGAGAAGTTGAATCAATAGTTCACGTAGTAAGATGTTTTGATGA
CGAAAATGTTGTTCACGTTGAAGGTACTGTTAATCCAATAAGAGATATTGAAACTATAAACTTAGAATTAATTTTTTCAG
ATTTAGAAGTTTTAGAAAGAAGAATGGAAAAAGGAATAAAACTTGCAAGATCTGGTGATAAAACTGCTAAGTTCGAATAT
GGAATCATGGAAAAAATTAAAGAACAATTGGAATCTAATAAACCAGCAAGAACTTTAGAATTTACAGATGAAGAGCAAGC
TTTTGTAAAAGGATTATTCTTAATTACTTCAAAGCCTGTTTTATATGCTTGTAATATTTCTGAAGATGATGTTATGGAAG
GAAACTTCGAAAATAAATATGTGAAGACAGTAAAAGAATATGCAGCATCTGAAAATTCAGAAGTAATAGTTGTAAGTGCT
AAGATAGAAGAAGAAGTATCTGGTTTAGAAGAAGATGAAAAGAATGAAATGTTAAGAGAATATGGTTTGGAAGAATCTGG
ACTTGATAAGTTAATTCAAGCAAGTTATAAATTATTAGGACTTATGAGTTTCTTAACAGCTGGAGTTCAAGAAGTTAGAG
CTTGGACTATAAAAAGAGGAACTAAAGCACCACAAGCGGCTAGTAAAATTCATACTGATATTGAAAGAGGATTTATTAGA
GCAGAAGTTGTTGGGTATAATGATTTAGTTGAATGTGGATCAGAAGCAGCAGCTAAAGAAAAAGGTAAGTTCAGACTTGA
GGGGAAAGATTACATAATGGAAGACGGAGATGTAGTTAACTTCAGATTTAACGTATAA

Upstream 100 bases:

>100_bases
AGCCTCATACTATAACATCAAACATATTTAGTTGAAATGAAAGACGTTCTAGAGTATAATTTTTAAAGGTGTATTAAATG
TTAGAAAGGACTGTACATAA

Downstream 100 bases:

>100_bases
CATAATTTTTTTAATAATAAAATAATAAAAGGAACCTTTGTTTGAGGGTTCCTTTTATTATTAAAAAAATTAAACTTTTG
TTATGTAAGGATATATTTAT

Product: GTP-dependent nucleic acid-binding protein EngD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 365; Mature: 365

Protein sequence:

>365_residues
MKLGIVGLPNVGKSTLFNAITKAGAESANYPFCTIEPNVGVVSVPDKRLDVLEKMYNTKKKIYTAIEFYDIAGLVKGASK
GEGLGNKFLANIREVESIVHVVRCFDDENVVHVEGTVNPIRDIETINLELIFSDLEVLERRMEKGIKLARSGDKTAKFEY
GIMEKIKEQLESNKPARTLEFTDEEQAFVKGLFLITSKPVLYACNISEDDVMEGNFENKYVKTVKEYAASENSEVIVVSA
KIEEEVSGLEEDEKNEMLREYGLEESGLDKLIQASYKLLGLMSFLTAGVQEVRAWTIKRGTKAPQAASKIHTDIERGFIR
AEVVGYNDLVECGSEAAAKEKGKFRLEGKDYIMEDGDVVNFRFNV

Sequences:

>Translated_365_residues
MKLGIVGLPNVGKSTLFNAITKAGAESANYPFCTIEPNVGVVSVPDKRLDVLEKMYNTKKKIYTAIEFYDIAGLVKGASK
GEGLGNKFLANIREVESIVHVVRCFDDENVVHVEGTVNPIRDIETINLELIFSDLEVLERRMEKGIKLARSGDKTAKFEY
GIMEKIKEQLESNKPARTLEFTDEEQAFVKGLFLITSKPVLYACNISEDDVMEGNFENKYVKTVKEYAASENSEVIVVSA
KIEEEVSGLEEDEKNEMLREYGLEESGLDKLIQASYKLLGLMSFLTAGVQEVRAWTIKRGTKAPQAASKIHTDIERGFIR
AEVVGYNDLVECGSEAAAKEKGKFRLEGKDYIMEDGDVVNFRFNV
>Mature_365_residues
MKLGIVGLPNVGKSTLFNAITKAGAESANYPFCTIEPNVGVVSVPDKRLDVLEKMYNTKKKIYTAIEFYDIAGLVKGASK
GEGLGNKFLANIREVESIVHVVRCFDDENVVHVEGTVNPIRDIETINLELIFSDLEVLERRMEKGIKLARSGDKTAKFEY
GIMEKIKEQLESNKPARTLEFTDEEQAFVKGLFLITSKPVLYACNISEDDVMEGNFENKYVKTVKEYAASENSEVIVVSA
KIEEEVSGLEEDEKNEMLREYGLEESGLDKLIQASYKLLGLMSFLTAGVQEVRAWTIKRGTKAPQAASKIHTDIERGFIR
AEVVGYNDLVECGSEAAAKEKGKFRLEGKDYIMEDGDVVNFRFNV

Specific function: GTP-dependent nucleic acid-binding protein which may act as a translation factor [H]

COG id: COG0012

COG function: function code J; Predicted GTPase, probable translation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI58761500, Length=370, Percent_Identity=45.945945945946, Blast_Score=319, Evalue=2e-87,
Organism=Homo sapiens, GI58761502, Length=220, Percent_Identity=43.1818181818182, Blast_Score=171, Evalue=1e-42,
Organism=Escherichia coli, GI1787454, Length=365, Percent_Identity=54.7945205479452, Blast_Score=410, Evalue=1e-116,
Organism=Escherichia coli, GI1789574, Length=134, Percent_Identity=32.089552238806, Blast_Score=72, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17509631, Length=367, Percent_Identity=43.8692098092643, Blast_Score=320, Evalue=5e-88,
Organism=Caenorhabditis elegans, GI17508313, Length=99, Percent_Identity=36.3636363636364, Blast_Score=68, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6319499, Length=371, Percent_Identity=43.3962264150943, Blast_Score=286, Evalue=5e-78,
Organism=Saccharomyces cerevisiae, GI6321773, Length=381, Percent_Identity=38.0577427821522, Blast_Score=231, Evalue=1e-61,
Organism=Saccharomyces cerevisiae, GI6321649, Length=350, Percent_Identity=27.7142857142857, Blast_Score=90, Evalue=6e-19,
Organism=Drosophila melanogaster, GI24640873, Length=376, Percent_Identity=44.9468085106383, Blast_Score=300, Evalue=1e-81,
Organism=Drosophila melanogaster, GI24640877, Length=376, Percent_Identity=44.9468085106383, Blast_Score=300, Evalue=1e-81,
Organism=Drosophila melanogaster, GI24640875, Length=376, Percent_Identity=44.9468085106383, Blast_Score=300, Evalue=1e-81,
Organism=Drosophila melanogaster, GI24640879, Length=335, Percent_Identity=43.5820895522388, Blast_Score=243, Evalue=1e-64,
Organism=Drosophila melanogaster, GI24585318, Length=142, Percent_Identity=32.3943661971831, Blast_Score=72, Evalue=8e-13,

Paralogues:

None

Copy number: 120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012675
- InterPro:   IPR004396
- InterPro:   IPR013029
- InterPro:   IPR006073
- InterPro:   IPR002917
- InterPro:   IPR012676
- InterPro:   IPR023192 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF06071 YchF-GTPase_C [H]

EC number: NA

Molecular weight: Translated: 40758; Mature: 40758

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLGIVGLPNVGKSTLFNAITKAGAESANYPFCTIEPNVGVVSVPDKRLDVLEKMYNTKK
CCEEEECCCCCCHHHHHHHHHHHCCCCCCCCEEEECCCCCEEECCHHHHHHHHHHHHHHH
KIYTAIEFYDIAGLVKGASKGEGLGNKFLANIREVESIVHVVRCFDDENVVHVEGTVNPI
HHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCC
RDIETINLELIFSDLEVLERRMEKGIKLARSGDKTAKFEYGIMEKIKEQLESNKPARTLE
CCCEEEEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHCCCCCEEEE
FTDEEQAFVKGLFLITSKPVLYACNISEDDVMEGNFENKYVKTVKEYAASENSEVIVVSA
CCCHHHHHHHHHHEEECCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEE
KIEEEVSGLEEDEKNEMLREYGLEESGLDKLIQASYKLLGLMSFLTAGVQEVRAWTIKRG
CHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
TKAPQAASKIHTDIERGFIRAEVVGYNDLVECGSEAAAKEKGKFRLEGKDYIMEDGDVVN
CCCCHHHHHHHHHHHHCEEEEEEECCHHHHHCCCHHHHHCCCCEEECCCCEEECCCCEEE
FRFNV
EEECC
>Mature Secondary Structure
MKLGIVGLPNVGKSTLFNAITKAGAESANYPFCTIEPNVGVVSVPDKRLDVLEKMYNTKK
CCEEEECCCCCCHHHHHHHHHHHCCCCCCCCEEEECCCCCEEECCHHHHHHHHHHHHHHH
KIYTAIEFYDIAGLVKGASKGEGLGNKFLANIREVESIVHVVRCFDDENVVHVEGTVNPI
HHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCC
RDIETINLELIFSDLEVLERRMEKGIKLARSGDKTAKFEYGIMEKIKEQLESNKPARTLE
CCCEEEEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHCCCCCEEEE
FTDEEQAFVKGLFLITSKPVLYACNISEDDVMEGNFENKYVKTVKEYAASENSEVIVVSA
CCCHHHHHHHHHHEEECCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEE
KIEEEVSGLEEDEKNEMLREYGLEESGLDKLIQASYKLLGLMSFLTAGVQEVRAWTIKRG
CHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
TKAPQAASKIHTDIERGFIRAEVVGYNDLVECGSEAAAKEKGKFRLEGKDYIMEDGDVVN
CCCCHHHHHHHHHHHHCEEEEEEECCHHHHHCCCHHHHHCCCCEEECCCCEEECCCCEEE
FRFNV
EEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]