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The map label for this gene is actII-2 [H]

Identifier: 187930661

GI number: 187930661

Start: 3768764

End: 3770230

Strand: Reverse

Name: actII-2 [H]

Synonym: Rpic_3597

Alternate gene names: 187930661

Gene position: 3770230-3768764 (Counterclockwise)

Preceding gene: 187930663

Following gene: 187930660

Centisome position: 95.63

GC content: 69.39

Gene sequence:

>1467_bases
GTGACCTGCCTTTCTTCTGCTTCCTCCGTATCCGAACCCCGCCGCTGGCTCGCCTTTGGAGTGATGGTGACGGCGCAATT
CATGTTCATCGTCGATGCGTTCATTGCCAATGTGGCGATCCCGAGCATCCGCACAAGCCTGCATGCCACACCCGCGCAAC
TCGAGGCCGTGCTGGCGGTGTACCAGCTTGGCTATGCGATTCTGCTCATCACTGGCGGCCGTCTGGGCGATCTGTTCGGT
CGCAGGCGCGTGTTCCTGCTCGGTCTCGTCGCCTTTACCGCCACCTCGGCCGGTTGTGGGCTGGCAGGCAGCGCGGCGGC
GCTGATCGCATGGCGTTTCGCGCAGGGCCTGTCGGCCGCGATGATGGTGCCGCAGGTGCTGGCGAGCGTGCAGGCGCTCT
TTGCCGGCGCCGAGCGCGACCGCGCGCTGTCGATTTTCGGCCTGGTGATGGGCACCGGCGGGGCGGCCGGGTTGCTGGTG
GGCGGCGCACTCACCAGCGCCGACATCGCCGGGCTGGGCTGGCGCTCGGCCTTCCTGATCAACGTGCCGGTCGGTTTGAC
TGCCGCGCTGCTTGGCTGGCGCTGGATTCCGCACCCGAGCGCCGACGCCGCAAAGGTGCATCTCGATACCGCCGGCGTGG
CGTGGATGGCCGTCAGCGTGGCCGCCGTGCTGGTGCCGCTGCTGTTCGGCCGCGAGCTGCACTGGCCGGTGTGGACCCTG
GCACTGCTGGTCGTAGGCGTGCTGGGCATGGCAACGTTCCAGCAGCTCGAGGCCCGTGTCGACCGCGCCGGCGGGACGCC
GCTGCTGCCGCCCACCTTGCTGCAGCATCGCGCCTTCATGACGGGCCTGACGGCGTCGGCGCTGCACTACATCGGGATGA
TGGCGTTCTTCCTGGTGCTGACGCTGTATCTGCAGAACGGGCGGCAGCTGTCTGCGGTGCGCATGGGCGTTGCCATCCTG
CCCCTGGCGATCACCTTTCTGATGACGTCGCGGGTGGCGAATGCGCTAGTGCGGCGCCATGGCGTTCGCGCGCTGCTGGC
GGGCCTCGGCCTGATGGCGTTGGGGCTGCTGGTGCTGCTGTCGGGCTTCGGTACGGAATGGGCGCACCCCGCCGCGCCGA
GCATGATGCGCATCGGGATTGCCGTGGCGCTGTACGGGGCAGGGCAAGGCCTGGTCGTCGTCCCGCTGATCGGCCTGGTG
CTGACGGGCGTTGCGCGTACGCAGGCCGGCGCAGCGGCGGGGCTGCTGATTACCGTGCAGCAGTTGGCAGGCGCACTGGG
CGTCGCCTGTATCGGCGGGCTGTATTTCACCTTTGCCGCTGCCGGCGGTGCCAACGGCATGGCGCGCGGCACGATGGCGG
GATGCATCGCCATGCTGGCAGCGCTCGGCGCAGCGGCCGTGGGCTTTGCGCGGCTCGGGGCACAGCAGCGGCAACTGGCG
GCCGCTAGCGCGGCAAGTCCGGCCTGA

Upstream 100 bases:

>100_bases
CGCAGGTGGGAGGGACAAGCTAATCCTAGGTTAATCCTAGCTCTGCATCGAATCGGCTCGCGCACCTACGCTGCGTTCAT
CCCTCTTTCCGGAGCCGATC

Downstream 100 bases:

>100_bases
GCCGCACCATCAAACCACTTCCACCATTCGATACTCGGGCGCGCGACACAAGCGCGCCCGTGTGCATTGATCGGCCGCAG
CGGTGATTCCACACTCCCGC

Product: major facilitator superfamily protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 488; Mature: 487

Protein sequence:

>488_residues
MTCLSSASSVSEPRRWLAFGVMVTAQFMFIVDAFIANVAIPSIRTSLHATPAQLEAVLAVYQLGYAILLITGGRLGDLFG
RRRVFLLGLVAFTATSAGCGLAGSAAALIAWRFAQGLSAAMMVPQVLASVQALFAGAERDRALSIFGLVMGTGGAAGLLV
GGALTSADIAGLGWRSAFLINVPVGLTAALLGWRWIPHPSADAAKVHLDTAGVAWMAVSVAAVLVPLLFGRELHWPVWTL
ALLVVGVLGMATFQQLEARVDRAGGTPLLPPTLLQHRAFMTGLTASALHYIGMMAFFLVLTLYLQNGRQLSAVRMGVAIL
PLAITFLMTSRVANALVRRHGVRALLAGLGLMALGLLVLLSGFGTEWAHPAAPSMMRIGIAVALYGAGQGLVVVPLIGLV
LTGVARTQAGAAAGLLITVQQLAGALGVACIGGLYFTFAAAGGANGMARGTMAGCIAMLAALGAAAVGFARLGAQQRQLA
AASAASPA

Sequences:

>Translated_488_residues
MTCLSSASSVSEPRRWLAFGVMVTAQFMFIVDAFIANVAIPSIRTSLHATPAQLEAVLAVYQLGYAILLITGGRLGDLFG
RRRVFLLGLVAFTATSAGCGLAGSAAALIAWRFAQGLSAAMMVPQVLASVQALFAGAERDRALSIFGLVMGTGGAAGLLV
GGALTSADIAGLGWRSAFLINVPVGLTAALLGWRWIPHPSADAAKVHLDTAGVAWMAVSVAAVLVPLLFGRELHWPVWTL
ALLVVGVLGMATFQQLEARVDRAGGTPLLPPTLLQHRAFMTGLTASALHYIGMMAFFLVLTLYLQNGRQLSAVRMGVAIL
PLAITFLMTSRVANALVRRHGVRALLAGLGLMALGLLVLLSGFGTEWAHPAAPSMMRIGIAVALYGAGQGLVVVPLIGLV
LTGVARTQAGAAAGLLITVQQLAGALGVACIGGLYFTFAAAGGANGMARGTMAGCIAMLAALGAAAVGFARLGAQQRQLA
AASAASPA
>Mature_487_residues
TCLSSASSVSEPRRWLAFGVMVTAQFMFIVDAFIANVAIPSIRTSLHATPAQLEAVLAVYQLGYAILLITGGRLGDLFGR
RRVFLLGLVAFTATSAGCGLAGSAAALIAWRFAQGLSAAMMVPQVLASVQALFAGAERDRALSIFGLVMGTGGAAGLLVG
GALTSADIAGLGWRSAFLINVPVGLTAALLGWRWIPHPSADAAKVHLDTAGVAWMAVSVAAVLVPLLFGRELHWPVWTLA
LLVVGVLGMATFQQLEARVDRAGGTPLLPPTLLQHRAFMTGLTASALHYIGMMAFFLVLTLYLQNGRQLSAVRMGVAILP
LAITFLMTSRVANALVRRHGVRALLAGLGLMALGLLVLLSGFGTEWAHPAAPSMMRIGIAVALYGAGQGLVVVPLIGLVL
TGVARTQAGAAAGLLITVQQLAGALGVACIGGLYFTFAAAGGANGMARGTMAGCIAMLAALGAAAVGFARLGAQQRQLAA
ASAASPA

Specific function: Promotes the efflux of actinorhodin [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI87081983, Length=438, Percent_Identity=30.1369863013699, Blast_Score=142, Evalue=7e-35,
Organism=Escherichia coli, GI1790195, Length=406, Percent_Identity=27.0935960591133, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1789042, Length=411, Percent_Identity=24.8175182481752, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1788710, Length=321, Percent_Identity=26.4797507788162, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1788392, Length=486, Percent_Identity=25.9259259259259, Blast_Score=85, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 49954; Mature: 49823

Theoretical pI: Translated: 11.64; Mature: 11.64

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTCLSSASSVSEPRRWLAFGVMVTAQFMFIVDAFIANVAIPSIRTSLHATPAQLEAVLAV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
YQLGYAILLITGGRLGDLFGRRRVFLLGLVAFTATSAGCGLAGSAAALIAWRFAQGLSAA
HHHCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCHHH
MMVPQVLASVQALFAGAERDRALSIFGLVMGTGGAAGLLVGGALTSADIAGLGWRSAFLI
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHCCHHHHHHHCCCCCCCEEEE
NVPVGLTAALLGWRWIPHPSADAAKVHLDTAGVAWMAVSVAAVLVPLLFGRELHWPVWTL
ECCHHHHHHHHCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
ALLVVGVLGMATFQQLEARVDRAGGTPLLPPTLLQHRAFMTGLTASALHYIGMMAFFLVL
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TLYLQNGRQLSAVRMGVAILPLAITFLMTSRVANALVRRHGVRALLAGLGLMALGLLVLL
HHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGFGTEWAHPAAPSMMRIGIAVALYGAGQGLVVVPLIGLVLTGVARTQAGAAAGLLITVQ
HCCCCCCCCCCCCHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
QLAGALGVACIGGLYFTFAAAGGANGMARGTMAGCIAMLAALGAAAVGFARLGAQQRQLA
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHH
AASAASPA
HHHCCCCC
>Mature Secondary Structure 
TCLSSASSVSEPRRWLAFGVMVTAQFMFIVDAFIANVAIPSIRTSLHATPAQLEAVLAV
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
YQLGYAILLITGGRLGDLFGRRRVFLLGLVAFTATSAGCGLAGSAAALIAWRFAQGLSAA
HHHCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCHHH
MMVPQVLASVQALFAGAERDRALSIFGLVMGTGGAAGLLVGGALTSADIAGLGWRSAFLI
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHCCHHHHHHHCCCCCCCEEEE
NVPVGLTAALLGWRWIPHPSADAAKVHLDTAGVAWMAVSVAAVLVPLLFGRELHWPVWTL
ECCHHHHHHHHCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
ALLVVGVLGMATFQQLEARVDRAGGTPLLPPTLLQHRAFMTGLTASALHYIGMMAFFLVL
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TLYLQNGRQLSAVRMGVAILPLAITFLMTSRVANALVRRHGVRALLAGLGLMALGLLVLL
HHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGFGTEWAHPAAPSMMRIGIAVALYGAGQGLVVVPLIGLVLTGVARTQAGAAAGLLITVQ
HCCCCCCCCCCCCHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
QLAGALGVACIGGLYFTFAAAGGANGMARGTMAGCIAMLAALGAAAVGFARLGAQQRQLA
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHH
AASAASPA
HHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1878971; 12000953 [H]