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The map label for this gene is rep [H]

Identifier: 187930550

GI number: 187930550

Start: 3645350

End: 3647470

Strand: Reverse

Name: rep [H]

Synonym: Rpic_3485

Alternate gene names: 187930550

Gene position: 3647470-3645350 (Counterclockwise)

Preceding gene: 187930558

Following gene: 187930548

Centisome position: 92.52

GC content: 62.14

Gene sequence:

>2121_bases
ATGTCTTCCGGTCTCGCCCACGGGCTCAATGCTGCCCAATCCGAAGGTGTGCATTACCTCGACGGCCCATGCCTGGTGCT
GGCCGGGGCGGGCTCGGGCAAGACGCGCGTGATCACGCAGAAGATCGCGCACCTGATCCTCGACAAGGGCTTCGAGCCGC
GTCACATCGCCGCCGTCACGTTCACCAACAAGGCGGCCAAGGAAATGCAGGAGCGCGTGGCCAAGCTGATGGACGGCAAG
ACGCGCGAAGACGGCAAGCGCATTCCCATCAAGCAGTTGACGGTCTGTACGTTCCACTCGCTGGGCGTGCAGATCCTGCG
GACCGAAGCCGAGCACGTGGGCCTGAAGCCGCGCTTCTCGATCATGGATTCGGACGACTGCTTCGGGATGATCCAGGAGC
AGTTGGCGACCACCGACAAGCAGTTGATCCGCCGCGTGCAGAGCACGATCTCGCTGTGGAAAAACGGTCTCGTCGATCCG
GACACCGCGATTGCCGAGGCGCTCGCCAACAACAATGTTGATGATCACCAGGCGGCGCTCGTCTACCGCAACTACGTGGC
GACGCTGCATGCGTATCAGGCGGTGGATTTTGACGACCTGATCCGGATTCCCGCCGAGCTGTTCGCGCGCAATGAAGAGG
TGCGCCTGAAGTGGCAGAACCGTCTGCGCTACTTCCTCGTCGATGAATACCAGGACACCAACGCTTGCCAGTATCAACTG
TTGAAGCTGCTGGCGGGGGGCTCGCACTTGCGTGCGCCGGCCTTCACGGCGGTGGGCGACGACGATCAGGCCATCTATGG
CTGGCGGGGCGCCACGCTCGACAACCTCAAGCTGCTGCAGACGGATTTCCCGAACCTGAAGGTGATCAAGCTGGAGCAGA
ACTACCGCTCCACGGTGCGTATTCTGAATGCCGCCAACGCGGTCATCGCACAGAACCCGAAGCTGTTCGAGAAGACGCTG
TGGAGCGAGCACGGCATGGGCGACGCGATCAACGTGACGAGCGCCAATGACGAGGAGCACGAGGCCGAGAGCGTCGTCTT
CAAGCTGTCCGCCCACAAGTTCGAGCGCCGCGCGCAGTTTCGGGATTACGCGATTCTGTATCGCGGCAACTTCCAGGCGC
GTCTGTTCGAGCAGATCCTGCGGCGCGAGCGCATTCCGTACGTGCTCTCGGGTGGGCAGAGCTTTTTCGACAAGGCCGAG
ATCAAGGATCTGTGCGCGTATCTGCGCCTGATTGCCAACGCGGATGATGATCCTGCGTTCATCCGCGCCATCACCACACC
CAAGCGCGGCGTGGGGAATGCGACGCTCGAAGTGCTGGGTTCGTTTGCGGGGCAGGCCAAGGTGTCGCTGTTCGAGGCGG
CGATGATGGGTGGTATCGAAGCACAGCTTGCGCCGCGCCAGCTTGAGCCGTTGCGCGTGTTCTGCGAGTTCATCGTGCGG
CTGTCGGACCGCGCCGCCAAGGAGCCTGCTGCCACGCTGCTCGACGAGATGATGGAAGGCATCCATTACGAGGCCTACCT
GTACGACACCTATGACGAGCGCCAGGCCCAGAACAAGTGGACCAACGTGCTGGAGTTTCTCGACTGGCTCAAGCGCAAGG
GCACCAAGCCGGAGAAGGAAAGCGAAGAAGACGCCACCGGTTTCGACAATGCCGATGGCCTGATGGACGCCGGCAAGAAC
CTGCTGGAACTCACGCAGACCATCGCGCTGATCAGCATGCTCGAAGGCCGGGAAGAGGACCCGGATGCGGTGCGCCTGTC
CACGCTGCATGCGAGCAAGGGGCTGGAGTATCCGCACGTGTTCCTGGTCGGCGTGGAAGAGGGCATCCTGCCGCACTGCC
GCGAAGACGATGAGTTGACCGACGAGAAGATCGAAGAAGAACGGCGGCTGATGTATGTGGGTATCACGCGGGCACAGCGC
AGCCTGCATATCAGCTGGTGCAAGAAGCGCAAGCGCGCGAGAGAGACGGTGGTGTGTGAGCCGTCGCGCTATATCGCCGA
GATGAAGCTTGGGGAAGATGCCGGCCCGACGCCGGAGGACACCATGCCGGCGATGTCGCCGAAGGATCGGTTGGGGGCGT
TGAAGGGGTTGTTGGCGGCTAAGAAACCTGTGGCTTCGTGA

Upstream 100 bases:

>100_bases
CCCGGCAAACGGCTTTTCATCGGCTCAATCGATGCAAACGCGCCGTGCTACCATCGCCCTCCGCTGTCGTTCCCGCCTGC
TTTTTCTTCCGCCTGTTCCC

Downstream 100 bases:

>100_bases
GGGGGTTTTGGTTTTTGGTTTTGGTGCATCCCTTGTTTCATCCCCTGCCGGGGCTGACTCACTTTCTTTGTCTTGCCAAA
GAAAGTAAGCAAAGAAAGGC

Product: UvrD/REP helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 706; Mature: 705

Protein sequence:

>706_residues
MSSGLAHGLNAAQSEGVHYLDGPCLVLAGAGSGKTRVITQKIAHLILDKGFEPRHIAAVTFTNKAAKEMQERVAKLMDGK
TREDGKRIPIKQLTVCTFHSLGVQILRTEAEHVGLKPRFSIMDSDDCFGMIQEQLATTDKQLIRRVQSTISLWKNGLVDP
DTAIAEALANNNVDDHQAALVYRNYVATLHAYQAVDFDDLIRIPAELFARNEEVRLKWQNRLRYFLVDEYQDTNACQYQL
LKLLAGGSHLRAPAFTAVGDDDQAIYGWRGATLDNLKLLQTDFPNLKVIKLEQNYRSTVRILNAANAVIAQNPKLFEKTL
WSEHGMGDAINVTSANDEEHEAESVVFKLSAHKFERRAQFRDYAILYRGNFQARLFEQILRRERIPYVLSGGQSFFDKAE
IKDLCAYLRLIANADDDPAFIRAITTPKRGVGNATLEVLGSFAGQAKVSLFEAAMMGGIEAQLAPRQLEPLRVFCEFIVR
LSDRAAKEPAATLLDEMMEGIHYEAYLYDTYDERQAQNKWTNVLEFLDWLKRKGTKPEKESEEDATGFDNADGLMDAGKN
LLELTQTIALISMLEGREEDPDAVRLSTLHASKGLEYPHVFLVGVEEGILPHCREDDELTDEKIEEERRLMYVGITRAQR
SLHISWCKKRKRARETVVCEPSRYIAEMKLGEDAGPTPEDTMPAMSPKDRLGALKGLLAAKKPVAS

Sequences:

>Translated_706_residues
MSSGLAHGLNAAQSEGVHYLDGPCLVLAGAGSGKTRVITQKIAHLILDKGFEPRHIAAVTFTNKAAKEMQERVAKLMDGK
TREDGKRIPIKQLTVCTFHSLGVQILRTEAEHVGLKPRFSIMDSDDCFGMIQEQLATTDKQLIRRVQSTISLWKNGLVDP
DTAIAEALANNNVDDHQAALVYRNYVATLHAYQAVDFDDLIRIPAELFARNEEVRLKWQNRLRYFLVDEYQDTNACQYQL
LKLLAGGSHLRAPAFTAVGDDDQAIYGWRGATLDNLKLLQTDFPNLKVIKLEQNYRSTVRILNAANAVIAQNPKLFEKTL
WSEHGMGDAINVTSANDEEHEAESVVFKLSAHKFERRAQFRDYAILYRGNFQARLFEQILRRERIPYVLSGGQSFFDKAE
IKDLCAYLRLIANADDDPAFIRAITTPKRGVGNATLEVLGSFAGQAKVSLFEAAMMGGIEAQLAPRQLEPLRVFCEFIVR
LSDRAAKEPAATLLDEMMEGIHYEAYLYDTYDERQAQNKWTNVLEFLDWLKRKGTKPEKESEEDATGFDNADGLMDAGKN
LLELTQTIALISMLEGREEDPDAVRLSTLHASKGLEYPHVFLVGVEEGILPHCREDDELTDEKIEEERRLMYVGITRAQR
SLHISWCKKRKRARETVVCEPSRYIAEMKLGEDAGPTPEDTMPAMSPKDRLGALKGLLAAKKPVAS
>Mature_705_residues
SSGLAHGLNAAQSEGVHYLDGPCLVLAGAGSGKTRVITQKIAHLILDKGFEPRHIAAVTFTNKAAKEMQERVAKLMDGKT
REDGKRIPIKQLTVCTFHSLGVQILRTEAEHVGLKPRFSIMDSDDCFGMIQEQLATTDKQLIRRVQSTISLWKNGLVDPD
TAIAEALANNNVDDHQAALVYRNYVATLHAYQAVDFDDLIRIPAELFARNEEVRLKWQNRLRYFLVDEYQDTNACQYQLL
KLLAGGSHLRAPAFTAVGDDDQAIYGWRGATLDNLKLLQTDFPNLKVIKLEQNYRSTVRILNAANAVIAQNPKLFEKTLW
SEHGMGDAINVTSANDEEHEAESVVFKLSAHKFERRAQFRDYAILYRGNFQARLFEQILRRERIPYVLSGGQSFFDKAEI
KDLCAYLRLIANADDDPAFIRAITTPKRGVGNATLEVLGSFAGQAKVSLFEAAMMGGIEAQLAPRQLEPLRVFCEFIVRL
SDRAAKEPAATLLDEMMEGIHYEAYLYDTYDERQAQNKWTNVLEFLDWLKRKGTKPEKESEEDATGFDNADGLMDAGKNL
LELTQTIALISMLEGREEDPDAVRLSTLHASKGLEYPHVFLVGVEEGILPHCREDDELTDEKIEEERRLMYVGITRAQRS
LHISWCKKRKRARETVVCEPSRYIAEMKLGEDAGPTPEDTMPAMSPKDRLGALKGLLAAKKPVAS

Specific function: Rep helicase is a single-stranded DNA-dependent ATPase involved in DNA replication; it can initiate unwinding at a nick in the DNA. It binds to the single-stranded DNA and acts in a progressive fashion along the DNA in the 3' to 5' direction [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI48994965, Length=713, Percent_Identity=46.984572230014, Blast_Score=574, Evalue=1e-165,
Organism=Escherichia coli, GI2367296, Length=675, Percent_Identity=35.4074074074074, Blast_Score=347, Evalue=1e-96,
Organism=Saccharomyces cerevisiae, GI6322369, Length=721, Percent_Identity=27.4618585298197, Blast_Score=201, Evalue=3e-52,
Organism=Saccharomyces cerevisiae, GI6324477, Length=673, Percent_Identity=23.1797919762259, Blast_Score=100, Evalue=7e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005752
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 79289; Mature: 79158

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSGLAHGLNAAQSEGVHYLDGPCLVLAGAGSGKTRVITQKIAHLILDKGFEPRHIAAVT
CCCCCHHCCCHHHHCCCEEECCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCEEEEEE
FTNKAAKEMQERVAKLMDGKTREDGKRIPIKQLTVCTFHSLGVQILRTEAEHVGLKPRFS
ECCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEE
IMDSDDCFGMIQEQLATTDKQLIRRVQSTISLWKNGLVDPDTAIAEALANNNVDDHQAAL
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCHHHHHH
VYRNYVATLHAYQAVDFDDLIRIPAELFARNEEVRLKWQNRLRYFLVDEYQDTNACQYQL
HHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHEEEHHCCCEEEEEECCCCCCHHHHHH
LKLLAGGSHLRAPAFTAVGDDDQAIYGWRGATLDNLKLLQTDFPNLKVIKLEQNYRSTVR
HHHHHCCCCCCCCEEEEECCCCCEEEEECCCCCCCCEEEECCCCCEEEEEECCCHHHHHH
ILNAANAVIAQNPKLFEKTLWSEHGMGDAINVTSANDEEHEAESVVFKLSAHKFERRAQF
HHHHCCHHHCCCCHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHH
RDYAILYRGNFQARLFEQILRRERIPYVLSGGQSFFDKAEIKDLCAYLRLIANADDDPAF
CCEEEEEECCCHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCE
IRAITTPKRGVGNATLEVLGSFAGQAKVSLFEAAMMGGIEAQLAPRQLEPLRVFCEFIVR
EEEECCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCHHCCCCCHHHHHHHHHHHHHH
LSDRAAKEPAATLLDEMMEGIHYEAYLYDTYDERQAQNKWTNVLEFLDWLKRKGTKPEKE
HHHHHCCCHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
SEEDATGFDNADGLMDAGKNLLELTQTIALISMLEGREEDPDAVRLSTLHASKGLEYPHV
CCCCCCCCCCCCHHHHHCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEHHHCCCCCCEE
FLVGVEEGILPHCREDDELTDEKIEEERRLMYVGITRAQRSLHISWCKKRKRARETVVCE
EEEECCCCCCCCCCCCCCCCHHHHHHHHCEEEEEEHHHHHHHHHHHHHHHHHHCCCEEEC
PSRYIAEMKLGEDAGPTPEDTMPAMSPKDRLGALKGLLAAKKPVAS
CHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SSGLAHGLNAAQSEGVHYLDGPCLVLAGAGSGKTRVITQKIAHLILDKGFEPRHIAAVT
CCCCHHCCCHHHHCCCEEECCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCEEEEEE
FTNKAAKEMQERVAKLMDGKTREDGKRIPIKQLTVCTFHSLGVQILRTEAEHVGLKPRFS
ECCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEE
IMDSDDCFGMIQEQLATTDKQLIRRVQSTISLWKNGLVDPDTAIAEALANNNVDDHQAAL
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCHHHHHH
VYRNYVATLHAYQAVDFDDLIRIPAELFARNEEVRLKWQNRLRYFLVDEYQDTNACQYQL
HHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHEEEHHCCCEEEEEECCCCCCHHHHHH
LKLLAGGSHLRAPAFTAVGDDDQAIYGWRGATLDNLKLLQTDFPNLKVIKLEQNYRSTVR
HHHHHCCCCCCCCEEEEECCCCCEEEEECCCCCCCCEEEECCCCCEEEEEECCCHHHHHH
ILNAANAVIAQNPKLFEKTLWSEHGMGDAINVTSANDEEHEAESVVFKLSAHKFERRAQF
HHHHCCHHHCCCCHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHH
RDYAILYRGNFQARLFEQILRRERIPYVLSGGQSFFDKAEIKDLCAYLRLIANADDDPAF
CCEEEEEECCCHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCE
IRAITTPKRGVGNATLEVLGSFAGQAKVSLFEAAMMGGIEAQLAPRQLEPLRVFCEFIVR
EEEECCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCHHCCCCCHHHHHHHHHHHHHH
LSDRAAKEPAATLLDEMMEGIHYEAYLYDTYDERQAQNKWTNVLEFLDWLKRKGTKPEKE
HHHHHCCCHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
SEEDATGFDNADGLMDAGKNLLELTQTIALISMLEGREEDPDAVRLSTLHASKGLEYPHV
CCCCCCCCCCCCHHHHHCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEHHHCCCCCCEE
FLVGVEEGILPHCREDDELTDEKIEEERRLMYVGITRAQRSLHISWCKKRKRARETVVCE
EEEECCCCCCCCCCCCCCCCHHHHHHHHCEEEEEEHHHHHHHHHHHHHHHHHHCCCEEEC
PSRYIAEMKLGEDAGPTPEDTMPAMSPKDRLGALKGLLAAKKPVAS
CHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]