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The map label for this gene is yfkN [H]

Identifier: 187930516

GI number: 187930516

Start: 3612485

End: 3614206

Strand: Reverse

Name: yfkN [H]

Synonym: Rpic_3451

Alternate gene names: 187930516

Gene position: 3614206-3612485 (Counterclockwise)

Preceding gene: 187930517

Following gene: 187930514

Centisome position: 91.67

GC content: 67.71

Gene sequence:

>1722_bases
ATGAACCGCCGCGAATTCGTTCAAGCCCTGGCGATTGCCGCAGCCGCCGGCCTGCGCCTGACCGACGCCCACGCAGATAC
GTTCAGCTACGACGTACCCCGTTTCGGCAACGTCCACCTGCTGCACTTCACCGACTGCCACGCGCAGTTGCTGCCCATCG
AATACCGCGAGCCCAACGTCAACCTCGGCGTGGCGCAATGGGCCGGGCAGCCGCCGCATCTGGTCGGGCACGCGCTGCTC
AAGCGTTACGGCATTGCGCCGGGCTCGCGCGCCGCGCACGCGCTCACGTCGCTCGACTTTACCGAGGCCGCGCGCCGCTA
CGGCCGCATGGGCGGCTTTGCCCACCTGGCCACGCTCATCAAGCGGCTGCGCGCCACGCGCCCGAATGCGCTGCTGCTGG
ACGGCGGCGACACCTGGCAGGGCTCCGCCACCGCGCTGTGGACCAACGGCCAGGACATGGTCGACGCCGCCCTCGCGCTG
GGCGTGGACGTCATGACCCCGCACTGGGAAATGACCTACGGCGCCGACCGAGTGCGCCACGTGGTCGATCACGATTTCAA
GAACAAGGTCGCCTTTGTCGCGCAGAACATCCAGACCGCCGACTTTGGTGATCCGGTGTTCGACCCGTATGTGCTGCGCG
AGCTGAACGGCGTGCCGGTCGCCATCATCGGGCAGGCGTTTCCGTACACGCCGATCGCGCACCCGGCCGATTTCACGCCC
GACTGGACCTTCGGCATTCAGGAAGAACGGCTGCAGAAGATGATTGACGAGGCGCGCGGCAAGGGCGCCAAGGTGGTTGT
GCTGCTGTCGCACAACGGCATGGATGTCGACCTGAAGCTCGCCTCGCGCGTGCGCGGGTTGGATGCCATCCTGGGCGGGC
ATACGCACGATGCCGTGCCCGCGCCCGTCAAGGTCAGCAACCCGGGCGGTGTGACGCTCGTCACCAACGCGGGCGCCAAC
GGCAAGTTTGTCAGCGTGCTCGATCTGGATGTGCGCGGCGGCACCGTGCGCGACATCCGCTATACGTTGCTGCCCGTGTT
TGCCGACCTCCTGCCGCCCGACCCCGCCATGACGGCGCTGATTGACCGCGTGCGCGCGCCGTACAAAGACAAGCTGGGCG
AAACGCTGGCCACCAACCGCGGCCTGCTGTACCGGCGCGGCAACTTCAACGGCACGTTCGACCAGCTCATCGTTGATGGG
CTGATGCACGCGCAGGGCGCCGAGATTGCCTTCTCGCCCGGCTTCCGCTGGGGCACCACGCTGCTGCCCGGCGAGGCCCT
GACCATGGAAGCGCTGATGGCCCAGACTGCCATCACGTACCCGGCCACCACGCTGACTGAGATGACCGGCGCCACCATCA
AGACCGTGCTGGAAGACGTGGCCGACAACCTGTTCAACCCAGACCCGTACTACCAGCAGGGCGGCGACATGGTGCGCACC
GGCGGCCTGCGCTACACCATCGACCCGATGAAGCCCATGGGCCAGCGCATTACCGACATGCGCTTAGGCCCGGGCGACGG
CAAGCCCATCGACGCCGACCGCCGCTACAAGGTGGCCGGCTGGGCCGCCGTGTCACCCGAAGCCCGCAAGGCCGGCGGCC
GCCCCGTATGGGACGTGCTGGCCGACTGGCTGCGCGACCAGCGCGAAGTGACCGCAAGGCCGCTGAACACGCCGCGCATT
GTCGGCATGGAGGGCAATCCGGGCATGGAAAACGCGGGTTAA

Upstream 100 bases:

>100_bases
TCGTGCTCGACGCCAAGGGCCGCGTGGTGCGCAAGGAAGTCGGCGAGATGCTGGAAGACGACGTGGCCGACCTCGCACGC
TACGCACACAACCCCAAGCA

Downstream 100 bases:

>100_bases
ACCCCCGATTGAAAGGGCCTGGGGAGTTCGGGTAGACTCATTTCCCACGGGCTGATAGCTCAGCTGGGAGAGCGCCGCGT
TCGCAATGCGGAGGTCGGGA

Product: 5'-Nucleotidase domain-containing protein

Products: NA

Alternate protein names: 2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase; 5'-nucleotidase [H]

Number of amino acids: Translated: 573; Mature: 573

Protein sequence:

>573_residues
MNRREFVQALAIAAAAGLRLTDAHADTFSYDVPRFGNVHLLHFTDCHAQLLPIEYREPNVNLGVAQWAGQPPHLVGHALL
KRYGIAPGSRAAHALTSLDFTEAARRYGRMGGFAHLATLIKRLRATRPNALLLDGGDTWQGSATALWTNGQDMVDAALAL
GVDVMTPHWEMTYGADRVRHVVDHDFKNKVAFVAQNIQTADFGDPVFDPYVLRELNGVPVAIIGQAFPYTPIAHPADFTP
DWTFGIQEERLQKMIDEARGKGAKVVVLLSHNGMDVDLKLASRVRGLDAILGGHTHDAVPAPVKVSNPGGVTLVTNAGAN
GKFVSVLDLDVRGGTVRDIRYTLLPVFADLLPPDPAMTALIDRVRAPYKDKLGETLATNRGLLYRRGNFNGTFDQLIVDG
LMHAQGAEIAFSPGFRWGTTLLPGEALTMEALMAQTAITYPATTLTEMTGATIKTVLEDVADNLFNPDPYYQQGGDMVRT
GGLRYTIDPMKPMGQRITDMRLGPGDGKPIDADRRYKVAGWAAVSPEARKAGGRPVWDVLADWLRDQREVTARPLNTPRI
VGMEGNPGMENAG

Sequences:

>Translated_573_residues
MNRREFVQALAIAAAAGLRLTDAHADTFSYDVPRFGNVHLLHFTDCHAQLLPIEYREPNVNLGVAQWAGQPPHLVGHALL
KRYGIAPGSRAAHALTSLDFTEAARRYGRMGGFAHLATLIKRLRATRPNALLLDGGDTWQGSATALWTNGQDMVDAALAL
GVDVMTPHWEMTYGADRVRHVVDHDFKNKVAFVAQNIQTADFGDPVFDPYVLRELNGVPVAIIGQAFPYTPIAHPADFTP
DWTFGIQEERLQKMIDEARGKGAKVVVLLSHNGMDVDLKLASRVRGLDAILGGHTHDAVPAPVKVSNPGGVTLVTNAGAN
GKFVSVLDLDVRGGTVRDIRYTLLPVFADLLPPDPAMTALIDRVRAPYKDKLGETLATNRGLLYRRGNFNGTFDQLIVDG
LMHAQGAEIAFSPGFRWGTTLLPGEALTMEALMAQTAITYPATTLTEMTGATIKTVLEDVADNLFNPDPYYQQGGDMVRT
GGLRYTIDPMKPMGQRITDMRLGPGDGKPIDADRRYKVAGWAAVSPEARKAGGRPVWDVLADWLRDQREVTARPLNTPRI
VGMEGNPGMENAG
>Mature_573_residues
MNRREFVQALAIAAAAGLRLTDAHADTFSYDVPRFGNVHLLHFTDCHAQLLPIEYREPNVNLGVAQWAGQPPHLVGHALL
KRYGIAPGSRAAHALTSLDFTEAARRYGRMGGFAHLATLIKRLRATRPNALLLDGGDTWQGSATALWTNGQDMVDAALAL
GVDVMTPHWEMTYGADRVRHVVDHDFKNKVAFVAQNIQTADFGDPVFDPYVLRELNGVPVAIIGQAFPYTPIAHPADFTP
DWTFGIQEERLQKMIDEARGKGAKVVVLLSHNGMDVDLKLASRVRGLDAILGGHTHDAVPAPVKVSNPGGVTLVTNAGAN
GKFVSVLDLDVRGGTVRDIRYTLLPVFADLLPPDPAMTALIDRVRAPYKDKLGETLATNRGLLYRRGNFNGTFDQLIVDG
LMHAQGAEIAFSPGFRWGTTLLPGEALTMEALMAQTAITYPATTLTEMTGATIKTVLEDVADNLFNPDPYYQQGGDMVRT
GGLRYTIDPMKPMGQRITDMRLGPGDGKPIDADRRYKVAGWAAVSPEARKAGGRPVWDVLADWLRDQREVTARPLNTPRI
VGMEGNPGMENAG

Specific function: Catalyzes the release of inorganic phosphate from 2',3'- cyclic nucleotides through consecutive 2',3'-phosphodiesterase and 3'- (or 2') nucleotidase activities. Also possesses a 5'- nucleotidase activity. Does not catalyze the release of inorganic phospha

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Secreted, cell wall; Peptidoglycan-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family [H]

Homologues:

Organism=Homo sapiens, GI4505467, Length=470, Percent_Identity=25.1063829787234, Blast_Score=72, Evalue=2e-12,
Organism=Escherichia coli, GI1790658, Length=217, Percent_Identity=26.2672811059908, Blast_Score=62, Evalue=7e-11,
Organism=Drosophila melanogaster, GI19921980, Length=435, Percent_Identity=22.5287356321839, Blast_Score=65, Evalue=9e-11,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR019931
- InterPro:   IPR004843
- InterPro:   IPR001899 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos [H]

EC number: =3.1.3.6; =3.1.4.16; =3.1.3.5 [H]

Molecular weight: Translated: 62359; Mature: 62359

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRREFVQALAIAAAAGLRLTDAHADTFSYDVPRFGNVHLLHFTDCHAQLLPIEYREPNV
CCHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCEEEEEEECCCCEEEEEEECCCCC
NLGVAQWAGQPPHLVGHALLKRYGIAPGSRAAHALTSLDFTEAARRYGRMGGFAHLATLI
CEEHHHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHCHHHHHHHHHHHCCCHHHHHHHH
KRLRATRPNALLLDGGDTWQGSATALWTNGQDMVDAALALGVDVMTPHWEMTYGADRVRH
HHHHCCCCCEEEEECCCCCCCCEEEEEECCHHHHHHHHHHCCCEECCCEEECCCHHHHHH
VVDHDFKNKVAFVAQNIQTADFGDPVFDPYVLRELNGVPVAIIGQAFPYTPIAHPADFTP
HHCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCC
DWTFGIQEERLQKMIDEARGKGAKVVVLLSHNGMDVDLKLASRVRGLDAILGGHTHDAVP
CCCCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCEEEEEHHHHHHHHHHHHCCCCCCCCC
APVKVSNPGGVTLVTNAGANGKFVSVLDLDVRGGTVRDIRYTLLPVFADLLPPDPAMTAL
CCEEECCCCCEEEEEECCCCCCEEEEEEEECCCCEEHHHHHHHHHHHHHHCCCCHHHHHH
IDRVRAPYKDKLGETLATNRGLLYRRGNFNGTFDQLIVDGLMHAQGAEIAFSPGFRWGTT
HHHHCCCHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCEEECCCCCCCCE
LLPGEALTMEALMAQTAITYPATTLTEMTGATIKTVLEDVADNLFNPDPYYQQGGDMVRT
ECCCCHHHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHCCCCEEEE
GGLRYTIDPMKPMGQRITDMRLGPGDGKPIDADRRYKVAGWAAVSPEARKAGGRPVWDVL
CCEEEEECCCCHHHHHHHHCEECCCCCCCCCCCCCEEEEEEEECCCHHHHCCCCCHHHHH
ADWLRDQREVTARPLNTPRIVGMEGNPGMENAG
HHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCC
>Mature Secondary Structure
MNRREFVQALAIAAAAGLRLTDAHADTFSYDVPRFGNVHLLHFTDCHAQLLPIEYREPNV
CCHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCEEEEEEECCCCEEEEEEECCCCC
NLGVAQWAGQPPHLVGHALLKRYGIAPGSRAAHALTSLDFTEAARRYGRMGGFAHLATLI
CEEHHHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHCHHHHHHHHHHHCCCHHHHHHHH
KRLRATRPNALLLDGGDTWQGSATALWTNGQDMVDAALALGVDVMTPHWEMTYGADRVRH
HHHHCCCCCEEEEECCCCCCCCEEEEEECCHHHHHHHHHHCCCEECCCEEECCCHHHHHH
VVDHDFKNKVAFVAQNIQTADFGDPVFDPYVLRELNGVPVAIIGQAFPYTPIAHPADFTP
HHCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCC
DWTFGIQEERLQKMIDEARGKGAKVVVLLSHNGMDVDLKLASRVRGLDAILGGHTHDAVP
CCCCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCEEEEEHHHHHHHHHHHHCCCCCCCCC
APVKVSNPGGVTLVTNAGANGKFVSVLDLDVRGGTVRDIRYTLLPVFADLLPPDPAMTAL
CCEEECCCCCEEEEEECCCCCCEEEEEEEECCCCEEHHHHHHHHHHHHHHCCCCHHHHHH
IDRVRAPYKDKLGETLATNRGLLYRRGNFNGTFDQLIVDGLMHAQGAEIAFSPGFRWGTT
HHHHCCCHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCEEECCCCCCCCE
LLPGEALTMEALMAQTAITYPATTLTEMTGATIKTVLEDVADNLFNPDPYYQQGGDMVRT
ECCCCHHHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHCCCCEEEE
GGLRYTIDPMKPMGQRITDMRLGPGDGKPIDADRRYKVAGWAAVSPEARKAGGRPVWDVL
CCEEEEECCCCHHHHHHHHCEECCCCCCCCCCCCCEEEEEEEECCCHHHHCCCCCHHHHH
ADWLRDQREVTARPLNTPRIVGMEGNPGMENAG
HHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]