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The map label for this gene is puuP [H]

Identifier: 187930491

GI number: 187930491

Start: 3591455

End: 3592825

Strand: Reverse

Name: puuP [H]

Synonym: Rpic_3425

Alternate gene names: 187930491

Gene position: 3592825-3591455 (Counterclockwise)

Preceding gene: 187930492

Following gene: 187930488

Centisome position: 91.13

GC content: 60.39

Gene sequence:

>1371_bases
ATGGACCAATCCGCCAAGCTCAATGCCGATTCCCTGGGTATCGTTGAATCGGTGATCATGGGCATCGCCGGTACTGCCCC
GGCCTACAGCATCGAGATCACGACTTCCACCATCATCGGGACGGCCGGCACGCAGTCGCCGGCCAGTATCCTGGTGTGCG
GGCTCATCATGTTTGGCATCGCGTACGCGTTCATCAACATGAACCGTGCCCTGGCGAGTGCCGGCACCTCGTATTCGTGG
GTGAGCATGGTATTCGGGCGCGCACTCGGCTTCTTTGCCGGCTGGGCGCTGCTGGTGCTGTGCTGCGTGTTCATGGTGTC
TGCCATGATTCCGGCGGCAAATGCCACGCTGCTCATCTTTGATCGGCCCATGATGAACAACGTGCACTACGTGACGTTGA
TCGCGGCGGCATGGCTCACGTTTGTCAGCGTGGTGGTCGTCAAGGGCATCAAGCTCACCAGCTACGTGCAGGTGCTGATG
ACGATTGTCGAGGGTGTCATTCTGCTGGGGATCATCGTCGCGAGTTTCATGGTGTTTCCGCGTACGCCGCAGCACCCGTT
CTCGTGGGTCTGGTTCTGGCCTTTCGCGTTCACACCCAAGCTGTTCGCCAACGGCGCGTTGGTGGCGATCTTCTTCTTTT
ACGGCTGGGACGTGACGATGAACCTCAGCGAAGAGACGCGCGACGCGAACCGCACGCCCGGCCGCGCCGCGTTCTGGTCG
ATGGTGTTCTTGATGGTGTTCTTCCTTGTCTTCATCGTCATCACGCAGCTCGGGCTGTCCGATGCCGAGATCCAGCACTA
CAACACGAACATCATCTTCGCCATTGCAGAGAAGCTGTTTGGGCCGACCTGGGGCTACGTCGCGATCGTCGCGGTCTTGC
TGAGCACGGTGGGAACCGTCGAAACCCAGATGCTGCAGTTCACGCGCACGCTCTTTGCCAAAAGCCGCGACGGCGCACTG
CACGCGCGCTATGCGCGCCTGCATCCGGTTTGGCGCACGCCGCACATCGCCATCTTCTTCATCTGGCTGACGGGGCTCGC
ACTGCTGTTCGTGTCGTCTTATCTGCCGACGATCAACGTCATCCTGCAGGATTCGATTACCGCCATCGGCTTCCAGATCT
GCTTCTACCTCGGGCTCACCGGGCTGGCTTGCGGCTGGTACTACCGCAAGGTGCTGACGCAGGGGCCGTGGGTCGCCATC
ACCCATGTCATCTGGCCGGTGGCAAGTGGGCTCTTCCTCTTCTTCATTGCGCTCTACAGCATCCCGACCTTCGACTGGGT
CACCAATGTGGTGGGCATGGGCGGCATCGCCATCGGTGTCATTCCGCTGCTGCTGAACCGTAAAAAGATCTGGTCGGCTC
GCTCCGCCTGA

Upstream 100 bases:

>100_bases
GACACCGCTCCAGCCCAGAGACGCCCCTATTGCCCGCCTATCCGGCACAGGGTAAAAATCGGTCTATTGCCCCCCAATCT
CGACTGGGAGTCGCGCATCC

Downstream 100 bases:

>100_bases
GCCAGTCCGATGCACCCGTTGCCATTCACCCGGCGCCAACCTGGGGCGCCGAATGCTGTCAGGCTGCGCTGACAGCGCCT
GCCGTATTCACCTACAGAAA

Product: amino acid permease-associated protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 456; Mature: 456

Protein sequence:

>456_residues
MDQSAKLNADSLGIVESVIMGIAGTAPAYSIEITTSTIIGTAGTQSPASILVCGLIMFGIAYAFINMNRALASAGTSYSW
VSMVFGRALGFFAGWALLVLCCVFMVSAMIPAANATLLIFDRPMMNNVHYVTLIAAAWLTFVSVVVVKGIKLTSYVQVLM
TIVEGVILLGIIVASFMVFPRTPQHPFSWVWFWPFAFTPKLFANGALVAIFFFYGWDVTMNLSEETRDANRTPGRAAFWS
MVFLMVFFLVFIVITQLGLSDAEIQHYNTNIIFAIAEKLFGPTWGYVAIVAVLLSTVGTVETQMLQFTRTLFAKSRDGAL
HARYARLHPVWRTPHIAIFFIWLTGLALLFVSSYLPTINVILQDSITAIGFQICFYLGLTGLACGWYYRKVLTQGPWVAI
THVIWPVASGLFLFFIALYSIPTFDWVTNVVGMGGIAIGVIPLLLNRKKIWSARSA

Sequences:

>Translated_456_residues
MDQSAKLNADSLGIVESVIMGIAGTAPAYSIEITTSTIIGTAGTQSPASILVCGLIMFGIAYAFINMNRALASAGTSYSW
VSMVFGRALGFFAGWALLVLCCVFMVSAMIPAANATLLIFDRPMMNNVHYVTLIAAAWLTFVSVVVVKGIKLTSYVQVLM
TIVEGVILLGIIVASFMVFPRTPQHPFSWVWFWPFAFTPKLFANGALVAIFFFYGWDVTMNLSEETRDANRTPGRAAFWS
MVFLMVFFLVFIVITQLGLSDAEIQHYNTNIIFAIAEKLFGPTWGYVAIVAVLLSTVGTVETQMLQFTRTLFAKSRDGAL
HARYARLHPVWRTPHIAIFFIWLTGLALLFVSSYLPTINVILQDSITAIGFQICFYLGLTGLACGWYYRKVLTQGPWVAI
THVIWPVASGLFLFFIALYSIPTFDWVTNVVGMGGIAIGVIPLLLNRKKIWSARSA
>Mature_456_residues
MDQSAKLNADSLGIVESVIMGIAGTAPAYSIEITTSTIIGTAGTQSPASILVCGLIMFGIAYAFINMNRALASAGTSYSW
VSMVFGRALGFFAGWALLVLCCVFMVSAMIPAANATLLIFDRPMMNNVHYVTLIAAAWLTFVSVVVVKGIKLTSYVQVLM
TIVEGVILLGIIVASFMVFPRTPQHPFSWVWFWPFAFTPKLFANGALVAIFFFYGWDVTMNLSEETRDANRTPGRAAFWS
MVFLMVFFLVFIVITQLGLSDAEIQHYNTNIIFAIAEKLFGPTWGYVAIVAVLLSTVGTVETQMLQFTRTLFAKSRDGAL
HARYARLHPVWRTPHIAIFFIWLTGLALLFVSSYLPTINVILQDSITAIGFQICFYLGLTGLACGWYYRKVLTQGPWVAI
THVIWPVASGLFLFFIALYSIPTFDWVTNVVGMGGIAIGVIPLLLNRKKIWSARSA

Specific function: Imports putrescine [H]

COG id: COG0531

COG function: function code E; Amino acid transporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amino acid-polyamine-organocation (APC) superfamily [H]

Homologues:

Organism=Escherichia coli, GI87081869, Length=363, Percent_Identity=24.2424242424242, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004841
- InterPro:   IPR002293 [H]

Pfam domain/function: PF00324 AA_permease [H]

EC number: NA

Molecular weight: Translated: 50263; Mature: 50263

Theoretical pI: Translated: 9.24; Mature: 9.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDQSAKLNADSLGIVESVIMGIAGTAPAYSIEITTSTIIGTAGTQSPASILVCGLIMFGI
CCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEEHEEEECCCCCCHHHHHHHHHHHHHH
AYAFINMNRALASAGTSYSWVSMVFGRALGFFAGWALLVLCCVFMVSAMIPAANATLLIF
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEE
DRPMMNNVHYVTLIAAAWLTFVSVVVVKGIKLTSYVQVLMTIVEGVILLGIIVASFMVFP
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
RTPQHPFSWVWFWPFAFTPKLFANGALVAIFFFYGWDVTMNLSEETRDANRTPGRAAFWS
CCCCCCCCEEEEECHHCCHHHHHCCHHHHHHHHHCCCEEEECCHHHCCCCCCCCHHHHHH
MVFLMVFFLVFIVITQLGLSDAEIQHYNTNIIFAIAEKLFGPTWGYVAIVAVLLSTVGTV
HHHHHHHHHHHHHHHHCCCCCCHHHHCCCCHHHEEHHHHCCCCHHHHHHHHHHHHHHHHH
ETQMLQFTRTLFAKSRDGALHARYARLHPVWRTPHIAIFFIWLTGLALLFVSSYLPTINV
HHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHH
ILQDSITAIGFQICFYLGLTGLACGWYYRKVLTQGPWVAITHVIWPVASGLFLFFIALYS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
IPTFDWVTNVVGMGGIAIGVIPLLLNRKKIWSARSA
CCCHHHHHHHHHCCHHHHHHHHHHHCHHHHHCCCCC
>Mature Secondary Structure
MDQSAKLNADSLGIVESVIMGIAGTAPAYSIEITTSTIIGTAGTQSPASILVCGLIMFGI
CCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEEHEEEECCCCCCHHHHHHHHHHHHHH
AYAFINMNRALASAGTSYSWVSMVFGRALGFFAGWALLVLCCVFMVSAMIPAANATLLIF
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEE
DRPMMNNVHYVTLIAAAWLTFVSVVVVKGIKLTSYVQVLMTIVEGVILLGIIVASFMVFP
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
RTPQHPFSWVWFWPFAFTPKLFANGALVAIFFFYGWDVTMNLSEETRDANRTPGRAAFWS
CCCCCCCCEEEEECHHCCHHHHHCCHHHHHHHHHCCCEEEECCHHHCCCCCCCCHHHHHH
MVFLMVFFLVFIVITQLGLSDAEIQHYNTNIIFAIAEKLFGPTWGYVAIVAVLLSTVGTV
HHHHHHHHHHHHHHHHCCCCCCHHHHCCCCHHHEEHHHHCCCCHHHHHHHHHHHHHHHHH
ETQMLQFTRTLFAKSRDGALHARYARLHPVWRTPHIAIFFIWLTGLALLFVSSYLPTINV
HHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHH
ILQDSITAIGFQICFYLGLTGLACGWYYRKVLTQGPWVAITHVIWPVASGLFLFFIALYS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
IPTFDWVTNVVGMGGIAIGVIPLLLNRKKIWSARSA
CCCHHHHHHHHHCCHHHHHHHHHHHCHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]