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The map label for this gene is bkdR [H]

Identifier: 187930453

GI number: 187930453

Start: 3547301

End: 3547789

Strand: Reverse

Name: bkdR [H]

Synonym: Rpic_3387

Alternate gene names: 187930453

Gene position: 3547789-3547301 (Counterclockwise)

Preceding gene: 187930455

Following gene: 187930452

Centisome position: 89.99

GC content: 62.17

Gene sequence:

>489_bases
ATGAATAAAGTGGAACTCGACAAGATCGACCGGAAGATCCTGGAAGTCCTGCAGAACAACGGCCGCCTCACCAACCTGGA
AGTCGCCGAGCGGGTGAATCTGTCGCCCAGCCCGTGTTTGCGACGCATCCGGCGCCTGGAAGAGTCCGGCGTCATCCGCC
AATACGCCGCGCTGCTCGACCCGTCCAAGATCGGGCTGGGGCTGTCGGCGTACATCAACGTGCGCCTGGAAAAGCAGGGC
GGCGCGCCCAAAGGGAAAGGCCCGTCCGACATCTTCCGCGCCGCCGTGGCGACATGGCCCGAGGTGGTCACCTGCTACGC
CATGACCGGCGAGATGGATTACCTGCTCAAGGTGTTCGTGGAAGACATGGAGCACTTCGCGCGCTTCATCCGCGATCAGT
TGCTCGCCCACCCGGCGGTGATCGATGTGAAGAGCAGTTTTGCGCTGGAGCGGATCAAGGATACGACGGCGTTGCCGGTG
GTGGTTTAA

Upstream 100 bases:

>100_bases
CAGTGTAGGTGTGTCCATCCAAGCAAAAATTGCGAAGTTGTGCCGACCTTGCTAAATTTGCGCAAGAAAATTGCCGAATT
AATTGGTGCGGAGCAACAAA

Downstream 100 bases:

>100_bases
GGGATTGGCTAGTTGCGCCATAAAGGCCTAAACCGCCGATGACGCACCTCAGCGGTCCGATCGTGGGCCTTCCTTGTTCG
AGCCTGAATCGCTGCGGGCG

Product: AsnC family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 162; Mature: 162

Protein sequence:

>162_residues
MNKVELDKIDRKILEVLQNNGRLTNLEVAERVNLSPSPCLRRIRRLEESGVIRQYAALLDPSKIGLGLSAYINVRLEKQG
GAPKGKGPSDIFRAAVATWPEVVTCYAMTGEMDYLLKVFVEDMEHFARFIRDQLLAHPAVIDVKSSFALERIKDTTALPV
VV

Sequences:

>Translated_162_residues
MNKVELDKIDRKILEVLQNNGRLTNLEVAERVNLSPSPCLRRIRRLEESGVIRQYAALLDPSKIGLGLSAYINVRLEKQG
GAPKGKGPSDIFRAAVATWPEVVTCYAMTGEMDYLLKVFVEDMEHFARFIRDQLLAHPAVIDVKSSFALERIKDTTALPV
VV
>Mature_162_residues
MNKVELDKIDRKILEVLQNNGRLTNLEVAERVNLSPSPCLRRIRRLEESGVIRQYAALLDPSKIGLGLSAYINVRLEKQG
GAPKGKGPSDIFRAAVATWPEVVTCYAMTGEMDYLLKVFVEDMEHFARFIRDQLLAHPAVIDVKSSFALERIKDTTALPV
VV

Specific function: Positive regulator of the bkd operon for branched-chain keto acid dehydrogenase complex [H]

COG id: COG1522

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH asnC-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081742, Length=155, Percent_Identity=41.9354838709677, Blast_Score=127, Evalue=4e-31,
Organism=Escherichia coli, GI1787116, Length=154, Percent_Identity=38.961038961039, Blast_Score=118, Evalue=2e-28,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011008
- InterPro:   IPR000485
- InterPro:   IPR019885
- InterPro:   IPR019888
- InterPro:   IPR019887
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01037 AsnC_trans_reg [H]

EC number: NA

Molecular weight: Translated: 18149; Mature: 18149

Theoretical pI: Translated: 8.70; Mature: 8.70

Prosite motif: PS00519 HTH_ASNC_1 ; PS50956 HTH_ASNC_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKVELDKIDRKILEVLQNNGRLTNLEVAERVNLSPSPCLRRIRRLEESGVIRQYAALLD
CCCCHHHHHHHHHHHHHHCCCCEEEHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHCC
PSKIGLGLSAYINVRLEKQGGAPKGKGPSDIFRAAVATWPEVVTCYAMTGEMDYLLKVFV
HHHHCCCEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
EDMEHFARFIRDQLLAHPAVIDVKSSFALERIKDTTALPVVV
HHHHHHHHHHHHHHHHCCCCEECHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MNKVELDKIDRKILEVLQNNGRLTNLEVAERVNLSPSPCLRRIRRLEESGVIRQYAALLD
CCCCHHHHHHHHHHHHHHCCCCEEEHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHCC
PSKIGLGLSAYINVRLEKQGGAPKGKGPSDIFRAAVATWPEVVTCYAMTGEMDYLLKVFV
HHHHCCCEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
EDMEHFARFIRDQLLAHPAVIDVKSSFALERIKDTTALPVVV
HHHHHHHHHHHHHHHHCCCCEECHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8320210 [H]