Click here to switch to the map view.

The map label for this gene is tuf1 [H]

Identifier: 187930366

GI number: 187930366

Start: 3435626

End: 3436834

Strand: Reverse

Name: tuf1 [H]

Synonym: Rpic_3299

Alternate gene names: 187930366

Gene position: 3436834-3435626 (Counterclockwise)

Preceding gene: 187930367

Following gene: 187930365

Centisome position: 87.17

GC content: 60.88

Gene sequence:

>1209_bases
ATGGCAAAGGAAAAGTTTGTACGGACCAAACCGCACGTGAACGTTGGTACGATCGGTCACGTTGACCACGGTAAGACCAC
GCTGACCGCAGCGATCGCAACCGTGCTGTCCGCCAAGTTTGGTGGCGAAGCGAAGAAGTACGACGAAATCGACGCAGCGC
CGGAAGAAAAGGCACGCGGCATCACGATCAACACCGCGCACGTCGAGTACGAGACGGCCAACCGTCACTATGCGCACGTT
GACTGCCCGGGCCACGCCGACTACGTCAAGAACATGATCACCGGTGCCGCCCAGATGGACGGTGCCATCCTGGTGTGCTC
GGCCGCTGACGGCCCGATGCCGCAAACGCGTGAGCACATCCTGCTGGCCCGTCAGGTCGGCGTGCCGTACATCATCGTCT
TCCTGAACAAGTGCGACATGGTGGACGACGCTGAATTGCTGGAACTGGTCGAGATGGAAGTGCGTGAACTTCTGTCGAAG
TATGAGTTCCCGGGCGACGACACCCCGATCATCAAGGGTTCGGCCAAGCTGGCGCTGGAAGGCGACAAGGGCGAACTGGG
CGAAGTGGCCATCATGAACCTGGCCGACGCACTGGACACCTACATCCCGACGCCGGAACGCGCAGTTGATGGTGCATTCC
TGATGCCGGTGGAAGACGTGTTCTCGATCTCGGGTCGCGGCACCGTGGTGACCGGCCGTATCGAGCGCGGCATCATCAAG
GTCGGCGAAGAAATCGAAATCGTCGGTATCGCCGTGGACGGCGACAAGCCGAAGATCGACAAGACCACCTGCACGGGCGT
GGAAATGTTCCGCAAGCTGCTGGACCAAGGTCAGGCAGGCGACAACGTCGGCATTCTGCTGCGCGGCACGAAGCGCGAAG
ATGTTCAGCGCGGTCAAGTGCTGGCCAAGCCGGGCTCGATCAAGCCGCACACGGAATTCACGGGCGAGGTCTACATCCTG
TCGAAGGACGAAGGCGGCCGTCACACGCCGTTCTTCAACAACTACCGTCCGCAGTTCTACTTCCGTACGACGGACGTGAC
CGGCTCGATCGCCTTGCCGGAAGGCAAGGAAATGGTCATGCCGGGCGACAACGTGTCGATCACCGTCAAGCTGATCGCCC
CGATCGCCATGGAAGAAGGTCTGCGCTTCGCCATCCGTGAAGGCGGCCGTACCGTGGGCGCTGGCGTCGTTGCCAAGATC
CTGAAGTAA

Upstream 100 bases:

>100_bases
TCCAGCGAAGGTAAGTAATCAATGTGTTTTGGGTGATCGCGTGTGCGATCGCCCTCAATCAATATCTAGCAATTAGCCCA
TTCCGAATTGAGGAGCTGAC

Downstream 100 bases:

>100_bases
AAGGCGGATCGATGCGCTGTTCCTCGTAAGGGGTGCGGCGCATCGCCTGTTGCATCGCTCTTTCAATCTACCGGCGGCCC
GACCGCCATGCTCTTTAAGG

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu 1 [H]

Number of amino acids: Translated: 402; Mature: 401

Protein sequence:

>402_residues
MAKEKFVRTKPHVNVGTIGHVDHGKTTLTAAIATVLSAKFGGEAKKYDEIDAAPEEKARGITINTAHVEYETANRHYAHV
DCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHILLARQVGVPYIIVFLNKCDMVDDAELLELVEMEVRELLSK
YEFPGDDTPIIKGSAKLALEGDKGELGEVAIMNLADALDTYIPTPERAVDGAFLMPVEDVFSISGRGTVVTGRIERGIIK
VGEEIEIVGIAVDGDKPKIDKTTCTGVEMFRKLLDQGQAGDNVGILLRGTKREDVQRGQVLAKPGSIKPHTEFTGEVYIL
SKDEGGRHTPFFNNYRPQFYFRTTDVTGSIALPEGKEMVMPGDNVSITVKLIAPIAMEEGLRFAIREGGRTVGAGVVAKI
LK

Sequences:

>Translated_402_residues
MAKEKFVRTKPHVNVGTIGHVDHGKTTLTAAIATVLSAKFGGEAKKYDEIDAAPEEKARGITINTAHVEYETANRHYAHV
DCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHILLARQVGVPYIIVFLNKCDMVDDAELLELVEMEVRELLSK
YEFPGDDTPIIKGSAKLALEGDKGELGEVAIMNLADALDTYIPTPERAVDGAFLMPVEDVFSISGRGTVVTGRIERGIIK
VGEEIEIVGIAVDGDKPKIDKTTCTGVEMFRKLLDQGQAGDNVGILLRGTKREDVQRGQVLAKPGSIKPHTEFTGEVYIL
SKDEGGRHTPFFNNYRPQFYFRTTDVTGSIALPEGKEMVMPGDNVSITVKLIAPIAMEEGLRFAIREGGRTVGAGVVAKI
LK
>Mature_401_residues
AKEKFVRTKPHVNVGTIGHVDHGKTTLTAAIATVLSAKFGGEAKKYDEIDAAPEEKARGITINTAHVEYETANRHYAHVD
CPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHILLARQVGVPYIIVFLNKCDMVDDAELLELVEMEVRELLSKY
EFPGDDTPIIKGSAKLALEGDKGELGEVAIMNLADALDTYIPTPERAVDGAFLMPVEDVFSISGRGTVVTGRIERGIIKV
GEEIEIVGIAVDGDKPKIDKTTCTGVEMFRKLLDQGQAGDNVGILLRGTKREDVQRGQVLAKPGSIKPHTEFTGEVYILS
KDEGGRHTPFFNNYRPQFYFRTTDVTGSIALPEGKEMVMPGDNVSITVKLIAPIAMEEGLRFAIREGGRTVGAGVVAKIL
K

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=400, Percent_Identity=57.75, Blast_Score=484, Evalue=1e-137,
Organism=Homo sapiens, GI4503475, Length=456, Percent_Identity=28.0701754385965, Blast_Score=155, Evalue=8e-38,
Organism=Homo sapiens, GI223555963, Length=435, Percent_Identity=29.8850574712644, Blast_Score=150, Evalue=1e-36,
Organism=Homo sapiens, GI5729864, Length=435, Percent_Identity=29.8850574712644, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI4503471, Length=456, Percent_Identity=27.8508771929825, Blast_Score=148, Evalue=7e-36,
Organism=Homo sapiens, GI194018522, Length=440, Percent_Identity=27.9545454545455, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI46094014, Length=445, Percent_Identity=27.1910112359551, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI194097354, Length=440, Percent_Identity=27.9545454545455, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI194018520, Length=440, Percent_Identity=27.9545454545455, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI54607086, Length=319, Percent_Identity=28.2131661442006, Blast_Score=108, Evalue=6e-24,
Organism=Homo sapiens, GI4503507, Length=363, Percent_Identity=28.099173553719, Blast_Score=92, Evalue=9e-19,
Organism=Homo sapiens, GI94966754, Length=135, Percent_Identity=32.5925925925926, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI1789737, Length=401, Percent_Identity=78.8029925187032, Blast_Score=623, Evalue=1e-180,
Organism=Escherichia coli, GI1790412, Length=401, Percent_Identity=78.8029925187032, Blast_Score=623, Evalue=1e-180,
Organism=Escherichia coli, GI2367247, Length=394, Percent_Identity=28.6802030456853, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI48994988, Length=277, Percent_Identity=31.4079422382672, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1789108, Length=314, Percent_Identity=28.9808917197452, Blast_Score=83, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17556456, Length=402, Percent_Identity=52.9850746268657, Blast_Score=413, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI25141371, Length=393, Percent_Identity=44.2748091603053, Blast_Score=325, Evalue=3e-89,
Organism=Caenorhabditis elegans, GI17552884, Length=464, Percent_Identity=30.3879310344828, Blast_Score=155, Evalue=4e-38,
Organism=Caenorhabditis elegans, GI17569207, Length=464, Percent_Identity=30.3879310344828, Blast_Score=155, Evalue=4e-38,
Organism=Caenorhabditis elegans, GI32566303, Length=445, Percent_Identity=28.7640449438202, Blast_Score=131, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI17506081, Length=279, Percent_Identity=30.1075268817204, Blast_Score=125, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI115532067, Length=440, Percent_Identity=26.8181818181818, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI115532065, Length=434, Percent_Identity=26.7281105990783, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI32566629, Length=357, Percent_Identity=28.2913165266106, Blast_Score=112, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI17509919, Length=306, Percent_Identity=25.4901960784314, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI32566301, Length=154, Percent_Identity=38.3116883116883, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17557151, Length=237, Percent_Identity=27.4261603375527, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6324761, Length=401, Percent_Identity=64.8379052369077, Blast_Score=525, Evalue=1e-150,
Organism=Saccharomyces cerevisiae, GI6325337, Length=452, Percent_Identity=29.646017699115, Blast_Score=147, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6319594, Length=452, Percent_Identity=29.646017699115, Blast_Score=147, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6322937, Length=341, Percent_Identity=27.2727272727273, Blast_Score=92, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6320377, Length=342, Percent_Identity=30.1169590643275, Blast_Score=90, Evalue=8e-19,
Organism=Saccharomyces cerevisiae, GI6320863, Length=286, Percent_Identity=26.9230769230769, Blast_Score=89, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6323098, Length=192, Percent_Identity=29.6875, Blast_Score=64, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6323320, Length=238, Percent_Identity=28.1512605042017, Blast_Score=63, Evalue=7e-11,
Organism=Drosophila melanogaster, GI281363316, Length=399, Percent_Identity=57.6441102756892, Blast_Score=479, Evalue=1e-135,
Organism=Drosophila melanogaster, GI17864358, Length=399, Percent_Identity=57.6441102756892, Blast_Score=479, Evalue=1e-135,
Organism=Drosophila melanogaster, GI19921738, Length=395, Percent_Identity=50.6329113924051, Blast_Score=397, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24652838, Length=461, Percent_Identity=29.0672451193059, Blast_Score=154, Evalue=1e-37,
Organism=Drosophila melanogaster, GI17137572, Length=461, Percent_Identity=29.0672451193059, Blast_Score=154, Evalue=1e-37,
Organism=Drosophila melanogaster, GI45553807, Length=461, Percent_Identity=29.0672451193059, Blast_Score=153, Evalue=3e-37,
Organism=Drosophila melanogaster, GI45553816, Length=461, Percent_Identity=29.0672451193059, Blast_Score=153, Evalue=3e-37,
Organism=Drosophila melanogaster, GI24651721, Length=461, Percent_Identity=29.0672451193059, Blast_Score=153, Evalue=3e-37,
Organism=Drosophila melanogaster, GI17864154, Length=461, Percent_Identity=29.0672451193059, Blast_Score=153, Evalue=3e-37,
Organism=Drosophila melanogaster, GI17137380, Length=346, Percent_Identity=30.635838150289, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI45550900, Length=441, Percent_Identity=27.891156462585, Blast_Score=118, Evalue=7e-27,
Organism=Drosophila melanogaster, GI19922690, Length=311, Percent_Identity=28.9389067524116, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI24647046, Length=269, Percent_Identity=27.8810408921933, Blast_Score=92, Evalue=5e-19,
Organism=Drosophila melanogaster, GI24647048, Length=269, Percent_Identity=27.8810408921933, Blast_Score=92, Evalue=6e-19,
Organism=Drosophila melanogaster, GI28572034, Length=243, Percent_Identity=31.6872427983539, Blast_Score=65, Evalue=8e-11,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 43560; Mature: 43428

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKEKFVRTKPHVNVGTIGHVDHGKTTLTAAIATVLSAKFGGEAKKYDEIDAAPEEKARG
CCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHCCC
ITINTAHVEYETANRHYAHVDCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHI
EEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHE
LLARQVGVPYIIVFLNKCDMVDDAELLELVEMEVRELLSKYEFPGDDTPIIKGSAKLALE
EEEHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCEEEEEE
GDKGELGEVAIMNLADALDTYIPTPERAVDGAFLMPVEDVFSISGRGTVVTGRIERGIIK
CCCCCCCCCHHHHHHHHHHHHCCCCCHHCCCEEEEEHHHHHCCCCCCEEEEECCCCCCEE
VGEEIEIVGIAVDGDKPKIDKTTCTGVEMFRKLLDQGQAGDNVGILLRGTKREDVQRGQV
CCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHCCEE
LAKPGSIKPHTEFTGEVYILSKDEGGRHTPFFNNYRPQFYFRTTDVTGSIALPEGKEMVM
EECCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEEECCCEEEECCCCCEEEE
PGDNVSITVKLIAPIAMEEGLRFAIREGGRTVGAGVVAKILK
CCCCCEEEEEEEECHHHHCCCEEEECCCCCEEHHHHHHHHCC
>Mature Secondary Structure 
AKEKFVRTKPHVNVGTIGHVDHGKTTLTAAIATVLSAKFGGEAKKYDEIDAAPEEKARG
CCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHCCC
ITINTAHVEYETANRHYAHVDCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHI
EEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHE
LLARQVGVPYIIVFLNKCDMVDDAELLELVEMEVRELLSKYEFPGDDTPIIKGSAKLALE
EEEHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCEEEEEE
GDKGELGEVAIMNLADALDTYIPTPERAVDGAFLMPVEDVFSISGRGTVVTGRIERGIIK
CCCCCCCCCHHHHHHHHHHHHCCCCCHHCCCEEEEEHHHHHCCCCCCEEEEECCCCCCEE
VGEEIEIVGIAVDGDKPKIDKTTCTGVEMFRKLLDQGQAGDNVGILLRGTKREDVQRGQV
CCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHCCEE
LAKPGSIKPHTEFTGEVYILSKDEGGRHTPFFNNYRPQFYFRTTDVTGSIALPEGKEMVM
EECCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEEECCCEEEECCCCCEEEE
PGDNVSITVKLIAPIAMEEGLRFAIREGGRTVGAGVVAKILK
CCCCCEEEEEEEECHHHHCCCEEEECCCCCEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA