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The map label for this gene is mlaA [H]

Identifier: 187930304

GI number: 187930304

Start: 3382208

End: 3383011

Strand: Reverse

Name: mlaA [H]

Synonym: Rpic_3237

Alternate gene names: 187930304

Gene position: 3383011-3382208 (Counterclockwise)

Preceding gene: 187930305

Following gene: 187930303

Centisome position: 85.81

GC content: 62.81

Gene sequence:

>804_bases
ATGAAATTAACAACATCCATCCGTATGCTGCGCGGCATTGCGCTGGCTCTGGCTGCAGGTACCACCCTGCTGGCCGGCTG
TGCGACGGGACCGAATGCTAATCCGGCTGACCCGATCGAACCGTTCAACCGCGAAGTCTTCCGCATCAACGAAGACCTCG
ACAAGGGCGTGCTGAAGCCAGTTGCGCAAACGTATGTCGATGTGGTGCCGTCGCCAGCGCGCACGGCGGTGTCGAATTTC
TTCTCGAATGCGGGCGATGTCTATTCGGCCGTCAACAACCTGCTGCAATTGAAGGGCACCGCCGCGCTGGAAGACACCAT
GCGCGTGGCGATCAATACCGTGCTGGGTCTGGGTGGCCTGATCGACATTGCGTCGGACGCCGGCCTGCCCAAGCACAAGG
AAGACTTCGGCCAGACGCTGGGCGTATGGGGTGTGCCGTCGGGTCCGTACGTCGTGTGGCCGCTGCTCGGCCCGAGCACT
GCACGTGACACCGTGGGGTTCCTGGTCGACTGGCAGATGGACCCGCTCACGTACTGGCACGACAGCGCGATCACCTACTC
GCTAGCCGCGTTGCGCGTGGTTGACGTGCGCGCGAACCTGCTCGGGGCCAGCAATGTGTTCGAACAGGCTGCGGTCGACA
AGTACACCTTCCTGCGCGATGCCTATCTGCAGCGCCGCCGCTACCTGATCTATGACGGCAACCTGCCGGAAGACCAGGAC
AACACGAAGTCGCCGGATACCGGTGACGCGACGGTCTCGCCGTACCATCGCTTCACCCCCAACTGGCCGGTGTTCCGCCG
CTGA

Upstream 100 bases:

>100_bases
GCCAGTTCCTCTATAGCAAGGCCGCGGATGCCGGTGCCGGCAGCGCGAATGGCAGTGCCGGCGGAGCCAAGCCGGCCGAG
GGCAGCGGGAGCAATAAGCC

Downstream 100 bases:

>100_bases
TCAGTACACGTAACAAGGTCGCCTCAAAGGTGACAAGGCGTAAAAGTGGCCGGCCCGCACGAACTTGGCTGCGGGCGGTC
TGTTCTAATCGCCAACAAGA

Product: VacJ family lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 267; Mature: 267

Protein sequence:

>267_residues
MKLTTSIRMLRGIALALAAGTTLLAGCATGPNANPADPIEPFNREVFRINEDLDKGVLKPVAQTYVDVVPSPARTAVSNF
FSNAGDVYSAVNNLLQLKGTAALEDTMRVAINTVLGLGGLIDIASDAGLPKHKEDFGQTLGVWGVPSGPYVVWPLLGPST
ARDTVGFLVDWQMDPLTYWHDSAITYSLAALRVVDVRANLLGASNVFEQAAVDKYTFLRDAYLQRRRYLIYDGNLPEDQD
NTKSPDTGDATVSPYHRFTPNWPVFRR

Sequences:

>Translated_267_residues
MKLTTSIRMLRGIALALAAGTTLLAGCATGPNANPADPIEPFNREVFRINEDLDKGVLKPVAQTYVDVVPSPARTAVSNF
FSNAGDVYSAVNNLLQLKGTAALEDTMRVAINTVLGLGGLIDIASDAGLPKHKEDFGQTLGVWGVPSGPYVVWPLLGPST
ARDTVGFLVDWQMDPLTYWHDSAITYSLAALRVVDVRANLLGASNVFEQAAVDKYTFLRDAYLQRRRYLIYDGNLPEDQD
NTKSPDTGDATVSPYHRFTPNWPVFRR
>Mature_267_residues
MKLTTSIRMLRGIALALAAGTTLLAGCATGPNANPADPIEPFNREVFRINEDLDKGVLKPVAQTYVDVVPSPARTAVSNF
FSNAGDVYSAVNNLLQLKGTAALEDTMRVAINTVLGLGGLIDIASDAGLPKHKEDFGQTLGVWGVPSGPYVVWPLLGPST
ARDTVGFLVDWQMDPLTYWHDSAITYSLAALRVVDVRANLLGASNVFEQAAVDKYTFLRDAYLQRRRYLIYDGNLPEDQD
NTKSPDTGDATVSPYHRFTPNWPVFRR

Specific function: Actively prevents phospholipid accumulation at the cell surface. Probably maintains lipid asymmetry in the outer membrane by retrograde trafficking of phospholipids from the outer membrane to the inner membrane [H]

COG id: COG2853

COG function: function code M; Surface lipoprotein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mlaA family [H]

Homologues:

Organism=Escherichia coli, GI1788688, Length=236, Percent_Identity=36.0169491525424, Blast_Score=130, Evalue=7e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007428 [H]

Pfam domain/function: PF04333 VacJ [H]

EC number: NA

Molecular weight: Translated: 29144; Mature: 29144

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLTTSIRMLRGIALALAAGTTLLAGCATGPNANPADPIEPFNREVFRINEDLDKGVLKP
CCCHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCCEEEECCHHHHHHHHH
VAQTYVDVVPSPARTAVSNFFSNAGDVYSAVNNLLQLKGTAALEDTMRVAINTVLGLGGL
HHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHH
IDIASDAGLPKHKEDFGQTLGVWGVPSGPYVVWPLLGPSTARDTVGFLVDWQMDPLTYWH
HHHHCCCCCCCCHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCCEEEEEEECCCCCEEEC
DSAITYSLAALRVVDVRANLLGASNVFEQAAVDKYTFLRDAYLQRRRYLIYDGNLPEDQD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCC
NTKSPDTGDATVSPYHRFTPNWPVFRR
CCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKLTTSIRMLRGIALALAAGTTLLAGCATGPNANPADPIEPFNREVFRINEDLDKGVLKP
CCCHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCCEEEECCHHHHHHHHH
VAQTYVDVVPSPARTAVSNFFSNAGDVYSAVNNLLQLKGTAALEDTMRVAINTVLGLGGL
HHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHH
IDIASDAGLPKHKEDFGQTLGVWGVPSGPYVVWPLLGPSTARDTVGFLVDWQMDPLTYWH
HHHHCCCCCCCCHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCCEEEEEEECCCCCEEEC
DSAITYSLAALRVVDVRANLLGASNVFEQAAVDKYTFLRDAYLQRRRYLIYDGNLPEDQD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCC
NTKSPDTGDATVSPYHRFTPNWPVFRR
CCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]