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The map label for this gene is hsdM [C]

Identifier: 187930243

GI number: 187930243

Start: 3330252

End: 3331763

Strand: Reverse

Name: hsdM [C]

Synonym: Rpic_3176

Alternate gene names: 187930243

Gene position: 3331763-3330252 (Counterclockwise)

Preceding gene: 187930244

Following gene: 187930242

Centisome position: 84.51

GC content: 59.13

Gene sequence:

>1512_bases
ATGAGTCGTATCAGTCAACAAGAACTCGAAAGCTACCTCTGGCGTGCTGCCGTTTTGCTGCGAGGGCTGATCGACGCTGG
CGACTACAAGCAGTTCATCTTTCCGCTGCTGTTCTTCAAGCGCGTGTCGGACGTGTGGGACGAGGAGTATCAGTCCGCTT
TGGTGGAGTCGGACGGCGACCTGTCCTACGCGCAGTTCGCCGAGAACCACCGCTTTCAGATCCCTGAGGGGGTGCACTGG
AACGATGTGCGGCAAACGCCGAAGAACGTTGGCAGCGCCATCCAGAAGGCCATGCGGGCCATCGAGTTGGCTAACCCTGA
CATGCTCGACGGCATTTTTGGAGATGCGTCGTGGACCAACCGCGAGCGTCTTCCGGATGAAACGCTGAAGGACCTGATCG
AGCACTTCTCAACTCAGACGCTGTCGGTGGCCAACGTCCCCGAGGATGAACTTGGTAACGCCTACGAGTACCTGATCAAG
AAGTTTGCGGATGACTCTGGTCACACGGCAGCGGAGTTCTACACCAACCGCACCGTCGTCCACTTAATGACGCAACTCTT
GGCACCGCTAGCGGGCGAGTCGATCTACGACCCCACGTGTGGCACCGGCGGCATGTTGATCTCGGCGTTGGACGAGGTGA
AGCGTTCGGGCGGCGAGTACCGCACCCTCACGCTCTACGGCCAAGAGCGCAACCTTATCACCTCCTCCATCGCGAGAATG
AACCTGTTCCTCCACGGCGTAGTGGACTTCGAGATCATCCGCGGCGACACCTTGGCCGAACCCAAGCACATCGAAGGCGA
CCGCCTGCGCCAGTTCGATGTGATCCTGGCCAACCCACCGTACTCGATTAAGCAATGGGATCGCGAGGCGTGGAGCAGTG
ACAAGTGGGGGCGCAACACCTTAGGCACGCCGCCGCAGGGTCGCGCCGATTACGCCTTTCATCAACACATCCTGACCAGC
CTCACTACCAAGGGCCGCTGTGCAGTGCTGTGGCCACACGGCGTTTTGTTCCGTAACGAGGAACAAGCCATGCGCGCAAA
GATGGTCGAGCAGGATTGGGTGGAGGCTGTGATTGGCTTGGGACCCAACCTGTTCTACAACTCCCCGATGGAGTCATGCG
TCGTCATCTGCAACCGCCGGAAGACGTCTGAACGCAAGGGCAAGGTGATCTTCATTGACGCGGTTAACGAAGTGACCCGC
GAGCGCGCTCAAAGCTTCCTCAAGCCTGAGCACCAACGGCGCATCTTGGCTGCCTACGAAGTGTTTGCGGACGAGCCTGG
CTTCGCCAAGGTCGCCACGCTGGCCGAGATTGGCGGCAACGGGGGCAACCTATCGATCCCGCTTTACGTGAAGCGCATTG
CCGCAGTGGTCGCCACTGACAGCAATGGCGATGCGGTATCACTCCGCTCTGCCTGGGATAAATGGCAAACGGATGGGCGC
ACCTTCTGGCAACAGATGGACACGTTGGTGGAAACGCTGGATGGGCTTTCCAAGGCTGAGGATATGCAATGA

Upstream 100 bases:

>100_bases
TGTCTGAAGCTGCAGCAATAGTGCTTCCTGCTGCCCGCAAGTTTCTCGCGAAACGGGTTTCGGATCTGGGCCTTCAGTTG
TGTTGGGAGAAAGGCAAAAA

Downstream 100 bases:

>100_bases
AGCTCCAAACGATTCGGCTATCCAACTTCCAGTCTTTCGGTGAGGCACCAACCGAAGTCGTGCTTGAAGACATCACCTAC
CTCATCGGGCCCAATGGATC

Product: N-6 DNA methylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 503; Mature: 502

Protein sequence:

>503_residues
MSRISQQELESYLWRAAVLLRGLIDAGDYKQFIFPLLFFKRVSDVWDEEYQSALVESDGDLSYAQFAENHRFQIPEGVHW
NDVRQTPKNVGSAIQKAMRAIELANPDMLDGIFGDASWTNRERLPDETLKDLIEHFSTQTLSVANVPEDELGNAYEYLIK
KFADDSGHTAAEFYTNRTVVHLMTQLLAPLAGESIYDPTCGTGGMLISALDEVKRSGGEYRTLTLYGQERNLITSSIARM
NLFLHGVVDFEIIRGDTLAEPKHIEGDRLRQFDVILANPPYSIKQWDREAWSSDKWGRNTLGTPPQGRADYAFHQHILTS
LTTKGRCAVLWPHGVLFRNEEQAMRAKMVEQDWVEAVIGLGPNLFYNSPMESCVVICNRRKTSERKGKVIFIDAVNEVTR
ERAQSFLKPEHQRRILAAYEVFADEPGFAKVATLAEIGGNGGNLSIPLYVKRIAAVVATDSNGDAVSLRSAWDKWQTDGR
TFWQQMDTLVETLDGLSKAEDMQ

Sequences:

>Translated_503_residues
MSRISQQELESYLWRAAVLLRGLIDAGDYKQFIFPLLFFKRVSDVWDEEYQSALVESDGDLSYAQFAENHRFQIPEGVHW
NDVRQTPKNVGSAIQKAMRAIELANPDMLDGIFGDASWTNRERLPDETLKDLIEHFSTQTLSVANVPEDELGNAYEYLIK
KFADDSGHTAAEFYTNRTVVHLMTQLLAPLAGESIYDPTCGTGGMLISALDEVKRSGGEYRTLTLYGQERNLITSSIARM
NLFLHGVVDFEIIRGDTLAEPKHIEGDRLRQFDVILANPPYSIKQWDREAWSSDKWGRNTLGTPPQGRADYAFHQHILTS
LTTKGRCAVLWPHGVLFRNEEQAMRAKMVEQDWVEAVIGLGPNLFYNSPMESCVVICNRRKTSERKGKVIFIDAVNEVTR
ERAQSFLKPEHQRRILAAYEVFADEPGFAKVATLAEIGGNGGNLSIPLYVKRIAAVVATDSNGDAVSLRSAWDKWQTDGR
TFWQQMDTLVETLDGLSKAEDMQ
>Mature_502_residues
SRISQQELESYLWRAAVLLRGLIDAGDYKQFIFPLLFFKRVSDVWDEEYQSALVESDGDLSYAQFAENHRFQIPEGVHWN
DVRQTPKNVGSAIQKAMRAIELANPDMLDGIFGDASWTNRERLPDETLKDLIEHFSTQTLSVANVPEDELGNAYEYLIKK
FADDSGHTAAEFYTNRTVVHLMTQLLAPLAGESIYDPTCGTGGMLISALDEVKRSGGEYRTLTLYGQERNLITSSIARMN
LFLHGVVDFEIIRGDTLAEPKHIEGDRLRQFDVILANPPYSIKQWDREAWSSDKWGRNTLGTPPQGRADYAFHQHILTSL
TTKGRCAVLWPHGVLFRNEEQAMRAKMVEQDWVEAVIGLGPNLFYNSPMESCVVICNRRKTSERKGKVIFIDAVNEVTRE
RAQSFLKPEHQRRILAAYEVFADEPGFAKVATLAEIGGNGGNLSIPLYVKRIAAVVATDSNGDAVSLRSAWDKWQTDGRT
FWQQMDTLVETLDGLSKAEDMQ

Specific function: The M And S Subunits Together Form A Methyltransferase (Mtase) That Methylates Two Adenine Residues In Complementary Strands Of A Bipartite DNA Recognition Sequence. In The Presence Of The R Subunit The Complex Can Also Act As An Endonuclease, Binding To

COG id: COG0286

COG function: function code V; Type I restriction-modification system methyltransferase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1790808, Length=401, Percent_Identity=23.1920199501247, Blast_Score=80, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022749
- InterPro:   IPR003356
- InterPro:   IPR002052
- InterPro:   IPR002296
- InterPro:   IPR011991 [H]

Pfam domain/function: PF12161 HsdM_N; PF02384 N6_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 56809; Mature: 56677

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRISQQELESYLWRAAVLLRGLIDAGDYKQFIFPLLFFKRVSDVWDEEYQSALVESDGD
CCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
LSYAQFAENHRFQIPEGVHWNDVRQTPKNVGSAIQKAMRAIELANPDMLDGIFGDASWTN
CHHHHHHCCCCCCCCCCCCHHHHHHCHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCC
RERLPDETLKDLIEHFSTQTLSVANVPEDELGNAYEYLIKKFADDSGHTAAEFYTNRTVV
CCCCCHHHHHHHHHHHCCCEEEECCCCHHHHCHHHHHHHHHHCCCCCCCHHHHHCCCHHH
HLMTQLLAPLAGESIYDPTCGTGGMLISALDEVKRSGGEYRTLTLYGQERNLITSSIARM
HHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHH
NLFLHGVVDFEIIRGDTLAEPKHIEGDRLRQFDVILANPPYSIKQWDREAWSSDKWGRNT
HHHHHHHEEEEEECCCCCCCCCCCCCCHHEEEEEEEECCCCHHHHHHHHHCCCCCCCCCC
LGTPPQGRADYAFHQHILTSLTTKGRCAVLWPHGVLFRNEEQAMRAKMVEQDWVEAVIGL
CCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCEEEECHHHHHHHHHHHHHHHHHHHHC
GPNLFYNSPMESCVVICNRRKTSERKGKVIFIDAVNEVTRERAQSFLKPEHQRRILAAYE
CCCCCCCCCHHHHHHHHCCCCCCCCCCCEEEEECHHHHHHHHHHHHHCCHHHHHHHHHHH
VFADEPGFAKVATLAEIGGNGGNLSIPLYVKRIAAVVATDSNGDAVSLRSAWDKWQTDGR
HHCCCCCHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHEECCCCCEEEHHHHHHHHCCCHH
TFWQQMDTLVETLDGLSKAEDMQ
HHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SRISQQELESYLWRAAVLLRGLIDAGDYKQFIFPLLFFKRVSDVWDEEYQSALVESDGD
CCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
LSYAQFAENHRFQIPEGVHWNDVRQTPKNVGSAIQKAMRAIELANPDMLDGIFGDASWTN
CHHHHHHCCCCCCCCCCCCHHHHHHCHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCC
RERLPDETLKDLIEHFSTQTLSVANVPEDELGNAYEYLIKKFADDSGHTAAEFYTNRTVV
CCCCCHHHHHHHHHHHCCCEEEECCCCHHHHCHHHHHHHHHHCCCCCCCHHHHHCCCHHH
HLMTQLLAPLAGESIYDPTCGTGGMLISALDEVKRSGGEYRTLTLYGQERNLITSSIARM
HHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHH
NLFLHGVVDFEIIRGDTLAEPKHIEGDRLRQFDVILANPPYSIKQWDREAWSSDKWGRNT
HHHHHHHEEEEEECCCCCCCCCCCCCCHHEEEEEEEECCCCHHHHHHHHHCCCCCCCCCC
LGTPPQGRADYAFHQHILTSLTTKGRCAVLWPHGVLFRNEEQAMRAKMVEQDWVEAVIGL
CCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCEEEECHHHHHHHHHHHHHHHHHHHHC
GPNLFYNSPMESCVVICNRRKTSERKGKVIFIDAVNEVTRERAQSFLKPEHQRRILAAYE
CCCCCCCCCHHHHHHHHCCCCCCCCCCCEEEEECHHHHHHHHHHHHHCCHHHHHHHHHHH
VFADEPGFAKVATLAEIGGNGGNLSIPLYVKRIAAVVATDSNGDAVSLRSAWDKWQTDGR
HHCCCCCHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHEECCCCCEEEHHHHHHHHCCCHH
TFWQQMDTLVETLDGLSKAEDMQ
HHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]